Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,637	C→T	T345T (ACC→ACT)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,637	0	C	T	97.1%	205.9 / ‑2.6	70	T345T (ACC→ACT)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (25/43);  total (25/45)
Fisher's exact test for biased strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

TCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAAA  >  NC_000913/4620390‑4620879                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   tCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCt                                                                                                                                                                                                                                                 >  1:548329/1‑251 (MQ=255) tCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGGTTGACGCCACCATAAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTAGTTGACTTCGACTCTTCCTGGGGCCACTGTCGCGACGTCGCCGGTTATGCGGCGTGTCTGGAGCTGTTCGACCGCCTTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGTTCATCACTGCt                                                                                                                                                                                                                                                 >  2:535314/1‑251 (MQ=255)    aCGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCt                                                                                                                                                                                                                                                 <  1:398506/248‑1 (MQ=255)    aCGCCAACTGCGGTAGCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGAc                                                                                                                                                                                                                                              <  1:635946/251‑1 (MQ=255)            cgCGGTATCACCAAGAAAGTGAAAGCCACTGGCCTGGACGCGCTGTTTGACTCCACCAGAAAAGAGATGAAAGAAGCG‑GTTGATACCACCATCGTCTTCACCATCGTCGTTGACTTCGACTCTTCCTGGGGCCCCCGTCGCGACGTCCCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTg                                                                                                                                                                                                                                      <  2:557871/250‑1 (MQ=255)              cGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATAGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCAGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTgtg                                                                                                                                                                                                                                    >  1:402279/1‑249 (MQ=255)                     aCCAAGAAATTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGAc                                                                                                                                                                                                                             <  1:160310/251‑1 (MQ=255)                           taagagAAAGCAACTGGCCTGGACGCGCTGTTTGACGCAACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACAGTCGCGACGTCGACGGGTTTGCTGCGGGTCCGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGATGCGCGATGACGACCTCCAGATCCACACTGCTAACCACGGTTgtgttcggccttgtgc                                                                                                                                                                                                                       >  2:157125/6‑236 (MQ=255)                                     caaCTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGAc                                                                                                                                                                                                             <  2:345111/249‑1 (MQ=255)                                      aaCTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGTTACTGAcc                                                                                                                                                                                                            >  1:540559/2‑251 (MQ=255)                                        cggtcctggaagttcTGTTCGACGCCACCATCAAAGAGATGAAAAAAGCG‑GGTGATAACATCAACGTCTTCACCAACTTCGTTGCCTTCGCCTCTTCCTGGGGCCACCTTCGCGACGTCGCCGTTTAGGTTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATTACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGAcctc                                                                                                                                                                                                          <  2:537280/237‑3 (MQ=255)                                        cTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGCGATGAACGAAGCG‑GGTGATAACACCATCGGCTTCACCAACTTCGTTGACTTCGACTCTTCATGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGATCTGGAGCGGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGGGATcagaacagcacagggactgacccc                                                                                                                                                                                                          >  2:310731/1‑228 (MQ=255)                                           gCCTGGACGCGCTGTTGGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACAcg                                                                                                                                                                                                       <  1:611779/251‑1 (MQ=255)                                            ccTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGGCCtagatcggacgagcgtcgggtt                                                                                                                                                                                                      >  2:209451/1‑230 (MQ=255)                                            ccTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCt                                                                                                                                                                                                                           <  1:209451/230‑1 (MQ=255)                                                  cgcgCTGTTTGACACCACAATCAAAGAGATGAAAGAAGAG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGCGCTGTTCGACCGCCGACTGCAGGAGCTGATGTCTCTGATGCGCGATGACGACATCATGCTCCTCAATGCAGACAACGGTTGTGATCCGACCTGGACCGGCAATGCCCACACGCGTAAac                                                                                                                                                                                                >  2:619381/1‑251 (MQ=255)                                                     gcggattGACGCCCCCAGCAAAGAGATGAACGCAGCG‑GGTGATAACACCATCGTATTAACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCCACGTCCCCGGTTCGGCGGCAGGTCTGGAGCTGTTCGCCCGCCATCTACCGGAGCTGCTGTCTCTGCTGCCCGATTACGACATACTGATCCTCTCTGCTGCCCACGGTTGTGATCCGACCTCGACAGGTAATAACCACACACGttcacac                                                                                                                                                                                              >  2:315180/6‑244 (MQ=255)                                                          ttGACGCCCCCATCAAAGAGATGAAGGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCTGCTCTTCCTGGGGCCACCGTCGCGACGGCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGGCCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTcc                                                                                                                                                                                         <  2:591204/250‑1 (MQ=255)                                                          ttGACGCCACCAACAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACGAACTTCGTTGACTTCGACCCTGCCTGGGGCCACCGTCGCGATGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTGCTACCGCCGTCTGCCGGAGCTGATGTCTCTGCGGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATTCGACCTGGACCGGTACTGACCACACGCGTGAACTCATTcc                                                                                                                                                                                         <  2:465348/250‑1 (MQ=255)                                                            gaagCCACCATCAAAGAGATGAAAGAAGTGTGGT‑ATAACCACATCGTCTTCACCAACTTCGTTGACTCCGCCGCTTCCTTGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGAGGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGt                                                                                                                                                                                      <  2:539849/248‑1 (MQ=255)                                                              ccccaccaTCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCATCACCAACTTCGTTGACTTCGTCTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTTTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCGCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGGTCCGACCTGGCACGGTACTGACCACGCGCGTGAACACATTCCTGTAc                                                                                                                                                                                    <  2:478383/249‑1 (MQ=255)                                                              cGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTAc                                                                                                                                                                                    >  1:159945/1‑251 (MQ=255)                                                                 caccaTCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTgg                                                                                                                                                                                 <  1:261468/251‑1 (MQ=255)                                                                  accaTCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGt                                                                                                                                                                                <  2:519052/251‑1 (MQ=255)                                                                       cAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGtttacg                                                                                                                                                                           <  1:558897/251‑6 (MQ=255)                                                                         aaGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGtttacggc                                                                                                                                                                         <  1:457009/251‑8 (MQ=255)                                                                           gagaTGAAAGAAGCG‑GGAGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGCCGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGccc                                                                                                                                                                       >  2:180261/1‑251 (MQ=255)                                                                                gAAAGAAGCG‑GGTGATAACACCAGCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACTGTCGCGACGCCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACAGACGTCTGCCGGAGCTGATGGCTCTGCTGCGCGATGACGACATCCTGATCCTAACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAg                                                                                                                                                                  <  1:657202/251‑1 (MQ=255)                                                                                 aaaGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGCAAGt                                                                                                                                                                 >  1:158440/1‑251 (MQ=255)                                                                                      agCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAAc                                                                                                                                                            <  1:293571/251‑1 (MQ=255)                                                                                                tAACACCATCGTCTTCACCCACTTCGTTTACTTCGGCTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGCCCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCATGATCCTCACTGCTGACCACGGTTGTGATCTGACCTGGACCGGTACTGAACACACGCGTGAACACATTCAGGTACTGGTATACGGCCCGGAAGTAACCCCGGGCTCAc                                                                                                                                                   >  2:392731/1‑251 (MQ=255)                                                                                                 aaCACCATCGTCTTCACCAACTTCGTTGACTTCGACTCGTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACt                                                                                                                                                  <  2:315824/251‑1 (MQ=255)                                                                                                          gTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCaccg                                                                                                                                         <  1:167342/251‑4 (MQ=255)                                                                                                               cACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCCCGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGACCTGGTTTACGGCCCGAAAGTaaaccc                                                                                                                                                           >  2:146055/1‑225 (MQ=255)                                                                                                               cACCAACTTCGGTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAAcc                                                                                                                                                           <  1:146055/228‑1 (MQ=255)                                                                                                                aCCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAAc                                                                                                                                   >  1:511092/1‑251 (MQ=255)                                                                                                                    actgcGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTc                                                                                                                               <  1:292990/247‑1 (MQ=255)                                                                                                                       tatttGACTTGGACACGTCCTGGGTCCACCGTCGCGACGTCGCCGGTTAGGCCGCGGGTCTGGGGCTGGTCGTCCGCCGTCTGCCTGAGCTGATGGCTCTGCTGCGCGATGACGACATCCTGATCCTGACTGCTGACCACTGTTGTGATCCGACCGGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTcg                                                                                                                              <  2:648744/246‑1 (MQ=255)                                                                                                                       tCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTcgc                                                                                                                             <  1:371858/250‑1 (MQ=255)                                                                                                                       tCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTcg                                                                                                                              <  1:240837/249‑1 (MQ=255)                                                                                                                        cGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTcgcg                                                                                                                            <  1:574922/250‑1 (MQ=255)                                                                                                                         gTTGACTTCGCCTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGa                                                                                                                          <  1:245407/251‑1 (MQ=255)                                                                                                                           tGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGat                                                                                                                         >  1:291151/1‑250 (MQ=255)                                                                                                                              cTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGCTCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAATACCGGGCTCACTGGGTCCCCGGGAAACCTTCCCGGattttt                                                                                                                     >  2:575972/1‑247 (MQ=255)                                                                                                                                 cGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGtt                                                                                                                                                                                                                                       <  1:564375/134‑1 (MQ=255)                                                                                                                                 cGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGtt                                                                                                                                                                                                                                       >  2:564375/1‑134 (MQ=255)                                                                                                                                 cGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGatattggt                                                                                                                   <  1:507597/250‑5 (MQ=255)                                                                                                                                 cGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGcc                                                                                                                  <  1:290173/251‑1 (MQ=255)                                                                                                                                 cGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATACTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGcc                                                                                                                  <  1:332042/251‑1 (MQ=255)                                                                                                                                      ctTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGAct                                                                                                             >  1:652716/1‑251 (MQ=255)                                                                                                                                         ccgggggCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACTCATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTg                                                                                                          <  2:342506/248‑1 (MQ=255)                                                                                                                                                 cACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGATCTGGTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAata                                                                                                  <  2:386476/251‑1 (MQ=255)                                                                                                                                                  aCCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGCCCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCCGCCTCTGGCAACatat                                                                                                 >  2:86529/1‑251 (MQ=255)                                                                                                                                                                  gggtgattctgctgggcgggagcTGTTCCACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCTATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAATCCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGata                                                                                 <  2:548329/229‑1 (MQ=255)                                                                                                                                                                            gcgGGTCTGGAGCTTTTCGACCGCCGTCTGCCGTATCTGATGTCTCTGCTGCGCGATGACGATGTCCTGATCCTCACTGCTTACCACGGTTGTGATCCGTCCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGGGAAACCTTCGCGGATATTGGTCAGACGCGGGCAAAATATTGTGGTACCGCTGATATGGAATATgg                                                                       <  2:329055/251‑1 (MQ=255)                                                                                                                                                                                gTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGACAAGGAATATGGCaa                                                                    >  2:170604/1‑250 (MQ=255)                                                                                                                                                                                    ggagcTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATACTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGATTACGGCCCGAAAGAAAAACCGGGATCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAACTATTTTGGTACTTCTGATATGGAATATGGCCAAGcc                                                                >  1:654227/5‑250 (MQ=255)                                                                                                                                                                                      gggctgtCGACCGCCGTCTGCCGCATCTGATGCCTCTGCTGCGCGATGACGACATCTTGATCCTCACTGCTGACCACGGGTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTTATATGGAATATGGCAAAGCCATg                                                             <  2:540559/245‑1 (MQ=255)                                                                                                                                                                                       gcTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGt                                                            <  1:555932/250‑1 (MQ=255)                                                                                                                                                                                          gTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTc                                                          <  1:170604/250‑1 (MQ=255)                                                                                                                                                                                                         gCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcg                                            <  1:60062‑M1/249‑11 (MQ=255)                                                                                                                                                                                                                  gATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccc                                 >  1:144726‑M1/1‑230 (MQ=255)                                                                                                                                                                                                                             tgcGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctccccccggcc                      >  1:233616‑M1/1‑219 (MQ=255)                                                                                                                                                                                                                                             ccttatcctCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTCACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggaga      <  2:159945‑M1/247‑49 (MQ=255)                                                                                                                                                                                                                                             ccTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggag       <  1:404521‑M1/250‑48 (MQ=255)                                                                                                                                                                                                                                                gATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTTAACACATTCCGGTACTGGTGTACGGCCCGAATGTAAAACCGGGCTCACTGGGGCACCGTGAAATCTTcgc                                                                                                                             <  2:307060/129‑1 (MQ=255)                                                                                                                                                                                                                                                gATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAACGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTcgc                                                                                                                             >  1:307060/1‑129 (MQ=255)                                                                                                                                                                                                                                                gATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagg    <  1:86529‑M1/250‑51 (MQ=255)                                                                                                                                                                                                                                                gATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATGCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACGGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagag     <  2:562635‑M1/249‑50 (MQ=255)                                                                                                                                                                                                                                                 aTCCTCACTGCTGACCACTGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggt  <  2:652716‑M1/251‑53 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   TCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAAA  >  NC_000913/4620390‑4620879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑