Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,134	G→A	A302A (GCG→GCA)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,134	0	G	A	95.2%	241.1 / 2.4	84	A302A (GCG→GCA)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (37/43);  total (39/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.93e-01

CCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGC  >  NC_000913/4618890‑4619374                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   ccGAAGCGATTGTTGCGGTGGCTACCGGCGCTGGCGTGCGCACCACCGCGCTGCCCACCGACATGAATCAGGTACTGGCCTCCAGGGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCTTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAAGTG‑CAAGCAGTGCTg                                                                                                                                                                                                                                              <  2:362594/251‑1 (MQ=255)    aaGCGATTGTGGGCGTGGCTAACGGCGCTGGCGTGCGCTCCACCGCGCTGCTCACCGAAATGAATCAGGTACTGGCCTCCAGGGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTgg                                                                                                                                                                                                                                             <  2:652778/249‑1 (MQ=255)      gCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACaa                                                                                                                                                                                                                                         <  1:366763/251‑1 (MQ=255)       cGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAAc                                                                                                                                                                                                                                        >  1:559928/1‑251 (MQ=255)                         aCGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGATCCTGACGGGTGAGGATCGAAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGCCGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGAAAACGGTaagacggccgaagac                                                                                                                                                                                                                      >  2:196144/1‑238 (MQ=255)                            gcgcTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCttt                                                                                                                                                                                                                   >  1:330476/1‑251 (MQ=255)                               cTGGCGTGCGCACCACCGCGCTGCTCACCGACATGACTCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGTCCAACTGGCGAAAGATGACGCCGCAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCCGAAGGCTTTGGt                                                                                                                                                                                                                >  2:112782/1‑251 (MQ=255)                                     tGCGCACCACCGCGCTGCTCACCGACATGAATCCGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATg                                                                                                                                                                                                          >  1:473201/1‑251 (MQ=255)                                     tGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATg                                                                                                                                                                                                          >  1:622657/1‑251 (MQ=255)                                         cccccccgagctgtgcACCGACATTGATCGGTTACTGGCCTCCAGTGCAGGTATCGCGGTTGAAGTTCGTGAAGCGGTGCAGTTGCTGACGGGTGAGTGTCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTTTGCGTGGTGATGCTTATCTCCGGCAAACTGGCGAAAGAGGACGCCGAAGCGCGCTCGTAATTG‑CAAGCAGTGCTGGACAACGGTATACCGGCAGAAGTCTTGGGTCGTATGGTAg                                                                                                                                                                                                      <  2:336807/236‑1 (MQ=255)                                         caccacCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAg                                                                                                                                                                                                      <  1:251017/251‑1 (MQ=255)                                         caccacCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAg                                                                                                                                                                                                      <  1:549798/251‑1 (MQ=255)                                          cccaccGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑c                                                                                                                                                                                                    <  1:566719/250‑1 (MQ=255)                                                  gctgctCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGACGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑aa                                                                                                                                                                                            >  2:546532/1‑250 (MQ=255)                                                  gctgctCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑aaa                                                                                                                                                                                           <  1:625342/251‑1 (MQ=255)                                                  gctgctCACCGAAATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGCCAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGAAC‑aa                                                                                                                                                                                            >  1:382488/1‑250 (MQ=255)                                                      ctCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAgg                                                                                                                                                                                       >  1:171774/1‑251 (MQ=255)                                                        cACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGcc                                                                                                                                                                                     <  1:588932/251‑1 (MQ=255)                                                          ccGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGccc                                                                                                                                                                                    >  1:274604/1‑250 (MQ=255)                                                            gACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGGCTTTGGGCGTATGGTAG‑CGGCACCAAAAAGGccg                                                                                                                                                                                    >  1:275355/1‑248 (MQ=255)                                                            gACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAGGCGGTGCTGGACAACGGGAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCccggg                                                                                                                                                                                 >  1:47426/1‑249 (MQ=255)                                                            gACATGAATCAGGTACTGGCCGCCAGGGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCccg                                                                                                                                                                                   <  2:275355/249‑1 (MQ=255)                                                            acaaTGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCccg                                                                                                                                                                                   <  2:47426/246‑1 (MQ=255)                                                                 gAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACt                                                                                                                                                                            >  1:480116/1‑251 (MQ=255)                                                                 gAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACt                                                                                                                                                                            <  1:109937/251‑1 (MQ=255)                                                                      aGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCATCCGACTTCGtt                                                                                                                                                                       <  1:577704/251‑1 (MQ=255)                                                                          aCTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTgaga                                                                                                                                                                   <  1:196144/251‑1 (MQ=255)                                                                          aCTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGAAGTTCCTGCCGGGTGAGTATCGTAACCCGCGTCTGTTTGCTGTCACGATGGCGCTGTGCGGGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGAAGCCGAAGCGCGAGAGAAATTGACAG‑CAATGCTGGACACCGGGAAAGCGGCAGAAGTCTTTGGGCGTATGG‑AGACGGCAC‑AAAAAGACCCAACCGAATTCGTTgtg                                                                                                                                                                    >  2:406726/1‑248 (MQ=255)                                                                           cTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTg                                                                                                                                                                                                                                              >  1:303906/1‑177 (MQ=255)                                                                           cTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTg                                                                                                                                                                                                                                              <  2:303906/177‑1 (MQ=255)                                                                                   cAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTAcgcg                                                                                                                                                           >  1:48413/1‑250 (MQ=255)                                                                                    aGTGCAGGTAACGCGGTTGACGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCAGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTACGGCAAACTGGCGAAAGATGACGCCGAAGCGAGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGACAAAAGACTTTGATCGTATGGTAA‑CGGCAC‑AAAAAGGCCCAACAGACTTCGtgtaggactagtcga                                                                                                                                                          >  2:327934/1‑236 (MQ=255)                                                                                    aGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGa                                                                                                                                                          >  1:135213/1‑250 (MQ=255)                                                                                      tGCAGGTACCGCGGTTGACGTTAGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGATGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTATTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGAATTAGTTGAGAACTACGCGAAgg                                                                                                                                                       >  2:320613/1‑250 (MQ=255)                                                                                      tGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGt                                                                                                                                                       >  1:176690/1‑251 (MQ=255)                                                                                         ccgattgtcgGTTGAAGTTCTGGAAGCGGTGCAGTTCCGGACGGGGGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATc                                                                                                                                                    <  2:601410/243‑1 (MQ=255)                                                                                             aaCGCGGTTGAAGTTCGAGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGcc                                                                                                                                                <  1:493174/251‑1 (MQ=255)                                                                                              aCGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCg                                                                                                                                               <  1:608535/251‑1 (MQ=255)                                                                                                gcgGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATc                                                                                                                                                    >  1:601410/1‑244 (MQ=255)                                                                                                 cgGTTGAAGTTCGTGAAGCTGGGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCGGTTTGATGTCACGATGGCTCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGTGAAATTG‑CAAGCAGTGCTGGACAACGGTAAATCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACa                                                                                                                                            <  2:675936/251‑1 (MQ=255)                                                                                                 cgGTTGAAGTTCGTGAAGCGGAGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGACTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGAAGAAGTATTTGGTCGTAAGATAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAATGATCTGCCGAcc                                                                                                                                            >  2:630675/1‑250 (MQ=255)                                                                                                            cGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGAc                                                                                                                                 <  1:204290/251‑1 (MQ=255)                                                                                                            cGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGAc                                                                                                                                 >  1:665587/1‑251 (MQ=255)                                                                                                                     gTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGt                                                                                                                        <  1:660192/251‑1 (MQ=255)                                                                                                                     gTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGATTATCGCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCa                                                                                                                          <  2:204937/249‑1 (MQ=255)                                                                                                                         agttccgtaCGGTTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGGTGGGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGGCTAt                                                                                                                    <  2:504690/243‑1 (MQ=255)                                                                                                                         aGTTCCTGACGGGAGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGCAGTATCTGCCGACCGCGATGCTGACGAAAGCAGTCTAt                                                                                                                    >  1:354986/1‑251 (MQ=255)                                                                                                                                gACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGAt                                                                                                              <  1:623405/250‑1 (MQ=255)                                                                                                                                 aCGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATAc                                                                                                            <  1:546532/251‑1 (MQ=255)                                                                                                                                 aCGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCACCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATAc                                                                                                            >  2:596344/1‑251 (MQ=255)                                                                                                                                           atcgataCCCGCGTCCGTTTGATGTCCCGATGGCGTTGTGCGTGGAGATGCTTATCTTCGGCAAACTGGCGAAAGATGACGCCGAAGCTCTCTCTAAAGTG‑CAAGCAGTGCTGGCCAACGGTAAAGCGGCATTAGTCTTTGGTCGTATGGTTT‑CGGCAC‑AACAAGGCCCAACCGACTTCGTTGAGATCTACGCGAAGTATCTGCCGACAGCGATGCTGACGAATGCAGCCTATGCTGATACCGAAGTtttt                                                                                                  <  2:508597/245‑1 (MQ=255)                                                                                                                                                accccGCGTCTGCTTGAAGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACGGGCGAAAGATGCCGCCGAAGCGCACGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGCACCAACCGACTTCTATGAGAAATAAGCGAAGTATCTGCCGACGGCACTGCTGACGACAGCAGTCTATGCTGCTACCGACGGTTTTGTc                                                                                               >  2:122481/3‑249 (MQ=255)                                                                                                                                                aacccttgTCTGTTTGATGTCACGAGGGCGCTGTGCGTGGAGATGCTTATCTCCTGCAATCTGGCGAAAGATGACGCCGAAGCGCGCGCGCAATTG‑CAAGCAGTGCTGGCCAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGTCTTCGTTTAGAACTACGCGAAGAAACTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCa                                                                                              <  2:181289/243‑1 (MQ=255)                                                                                                                                                  cccGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTg                                                                                           <  1:262608/251‑1 (MQ=255)                                                                                                                                                   ccGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGa                                                                                          <  1:257248/251‑1 (MQ=255)                                                                                                                                                       gTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTAc                                                                                                                                                              <  1:232524/179‑1 (MQ=255)                                                                                                                                                       gTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTAc                                                                                                                                                              >  2:232524/1‑179 (MQ=255)                                                                                                                                                             ttGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAGGGTTTTGTCAGTGAAATGGATAcc                                                                                >  2:300743/1‑251 (MQ=255)                                                                                                                                                             ttGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATAcc                                                                                >  1:592256/1‑251 (MQ=255)                                                                                                                                                             ttGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATAcc                                                                                >  1:252251/1‑251 (MQ=255)                                                                                                                                                               gATGTCACGATGGTGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGATGTATCTGCCGACAGCGATGCTGACGAAAGCAGGCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGAGACCcg                                                                              <  2:373146/251‑1 (MQ=255)                                                                                                                                                                  gTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGCCGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGaa                                                                                                                                                         >  2:650538/1‑173 (MQ=255)                                                                                                                                                                  gTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGaa                                                                                                                                                         <  1:650538/173‑1 (MQ=255)                                                                                                                                                                                       gagaTGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑c                                                                                                                                                                                                    <  2:194312/110‑1 (MQ=255)                                                                                                                                                                                       gagaTGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑c                                                                                                                                                                                                    >  1:194312/1‑110 (MQ=255)                                                                                                                                                                                             gccgaactcGGCAAACGTGCGAAAGATGCCGCCGAAGCGCGCGCGAAATTG‑CAAGCAGGGCTGGACAACGGTAAAGCGGCAGAAGTCGTTGGTCGGATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCaaggg                                                 <  2:302157/242‑4 (MQ=255)                                                                                                                                                                                              ttaTCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCTATATTG‑CAAGCAGTGCTGGCCAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGACCTACGCGCAGTATCGGCAGACAGCGCTGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGTAGGCAGTGGTTGCAATgggg                                               >  2:101410/3‑250 (MQ=255)                                                                                                                                                                                                aTCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGCTGGCAGTGGTGGCAAGGggcgg                                             >  2:259137/1‑251 (MQ=255)                                                                                                                                                                                                 tctcCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGt                                                                                                                        >  1:98937/1‑175 (MQ=255)                                                                                                                                                                                                 tctcCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCGTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGt                                                                                                                        <  2:98937/175‑1 (MQ=255)                                                                                                                                                                                                    ccGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGggcgga                                            <  2:559928/248‑2 (MQ=255)                                                                                                                                                                                                    ccGGCAAACGGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGcggcggt                                         <  2:274604/251‑2 (MQ=255)                                                                                                                                                                                                     ctgcaaacaagCGAAGGATGACGCCGAAGCGCGCGCGAAATTA‑CAAGCAGTGCTGGACCACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGGAG‑CGGCAC‑AAAAAGGCCCCACCGACTTCGTTGAGACCTACGCGCAGTATCTGACGACATCGATGCTGACGAAAGCAGTCTATGCTAATACCGAAAGGTTTGTCCGTGTAATGTATACCCGCGCGCTGGGGATGACAGTGGTTGCAATGGTcggcgggt                                        >  2:467374/11‑249 (MQ=255)                                                                                                                                                                                                     cGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATgg                                                 >  1:302157/1‑242 (MQ=255)                                                                                                                                                                                                        cAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGcggcggtcgtc                                     <  1:112782/251‑6 (MQ=255)                                                                                                                                                                                                              ggCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCTCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAgg                               <  1:521550/251‑1 (MQ=255)                                                                                                                                                                                                                 gAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCAt                            <  1:300743/251‑1 (MQ=255)                                                                                                                                                                                                                       ggACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGaca                      <  2:176690/250‑1 (MQ=255)                                                                                                                                                                                                                            ccGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCAtt                 <  2:171774/251‑2 (MQ=255)                                                                                                                                                                                                                            ccGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGACCATGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGCGAACTACACGGCGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTAAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGCCGTCGTCAGGCATCTGACACCAtt                 >  1:313234/1‑250 (MQ=255)                                                                                                                                                                                                                                      cgcgAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCg       <  1:6286/251‑1 (MQ=255)                                                                                                                                                                                                                                         gAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCg    >  1:84922/1‑251 (MQ=255)                                                                                                                                                                                                                                          aaaTTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCgg   <  1:74842/251‑1 (MQ=255)                                                                                                                                                                                                                                           aaTTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGc  <  1:96923/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   CCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGC  >  NC_000913/4618890‑4619374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑