Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,080	G→T	L284L (CTG→CTT)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,080	0	G	T	95.2%	247.6 / 5.2	83	L284L (CTG→CTT)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (39/40);  total (41/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.34e-02

AAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCG  >  NC_000913/4618839‑4619298                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                              aaGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGGGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGGCGGGGGAGTAACGTAACCCGCGTCTGTTTGATTTCACGATTGCGCTGTGCGTGGAGAAGCTTATCTCCGGc                                                                                                                                                                                                                      >  2:366763/1‑251 (MQ=255)   gTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCCCCGACATAAATCAGGGACTGGCCTCCAGTGCCGGGAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGGCGGGTGGGGATCTTAACCCGCGTCTGTTTGATGTCACGGTGGCCCTGTGCGTGGAGATTCTTATCTCCGGCa                                                                                                                                                                                                                     >  2:411152/1‑250 (MQ=255)             gcgcGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGaaa                                                                                                                                                                                                          <  1:542502/251‑1 (MQ=255)                 gTTTATGCCGACCTGCGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGGGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATg                                                                                                                                                                                                      >  1:504690/1‑251 (MQ=255)                 gTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATg                                                                                                                                                                                                      >  1:652778/1‑251 (MQ=255)                       gCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGcc                                                                                                                                                                                                 <  1:26380/250‑1 (MQ=255)                       gCCGACCTACGAACTCGCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCTACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGGCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCg                                                                                                                                                                                                <  1:611497/251‑1 (MQ=255)                            ccTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGTCAAACTGGCGAAAGATGACGCCGAAgcg                                                                                                                                                                                           >  1:498393/1‑251 (MQ=255)                            ccTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAgcg                                                                                                                                                                                           <  1:469526/251‑1 (MQ=255)                                  aaCTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGAAATGGCCTCCAGTGCAGGTAACGCGGCTGAAGTTCGTGAAGCGGTGCAGTTCATGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTAACTCCGTCAAACTGGCGAAAGCTGACGCAGAATcgcgcgcg                                                                                                                                                                                     >  1:57357/1‑251 (MQ=255)                                            aGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑Caag                                                                                                                                                                          <  1:45394/251‑3 (MQ=255)                                             gCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑Caagc                                                                                                                                                                         <  1:37382/251‑4 (MQ=255)                                               ccTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑Caagcag                                                                                                                                                                       <  1:376692/251‑6 (MQ=255)                                                  ggCCGAAGCGATTGTGGGCGTTGCTAACGGCGCTGGCGTGCGCTCCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑Caagcagtg                                                                                                                                                                     <  2:676389/249‑8 (MQ=255)                                                    ccGAAGCGATTGTTGCGGTGGCTACCGGCGCTGGCGTGCGCACCACCGCGCTGCCCACCGACATGAATCAGGTACTGGCCTCCAGGGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCTTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAAGTG‑CAAGCAGTGCTg                                                                                                                                                                  <  2:362594/251‑1 (MQ=255)                                                      gAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAAACCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGGGGAGATGCTTATCGCCGGCAAACTGGCGCAAGATGACGCCGAAGCGCGCGCGACATTG‑Caagcaaggctgg                                                                                                                                                                 >  2:109937/1‑239 (MQ=255)                                                       aaGCGATTGTGGGCGTGGCTAACGGCGCTGGCGTGCGCTCCACCGCGCTGCTCACCGAAATGAATCAGGTACTGGCCTCCAGGGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTgg                                                                                                                                                                 <  2:652778/249‑1 (MQ=255)                                                         gCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACaa                                                                                                                                                             <  1:366763/251‑1 (MQ=255)                                                          cGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAAc                                                                                                                                                            >  1:559928/1‑251 (MQ=255)                                                                            aCGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGATCCTGACGGGTGAGGATCGAAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGCCGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGAAAACGGTaagacggccgaagac                                                                                                                                          >  2:196144/1‑238 (MQ=255)                                                                               gcgcTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCttt                                                                                                                                       >  1:330476/1‑251 (MQ=255)                                                                                  cTGGCGTGCGCACCACCGCGCTGCTCACCGACATGACTCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGTCCAACTGGCGAAAGATGACGCCGCAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCCGAAGGCTTTGGt                                                                                                                                    >  2:112782/1‑251 (MQ=255)                                                                                        tGCGCACCACCGCGCTGCTCACCGACATGAATCCGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATg                                                                                                                              >  1:473201/1‑251 (MQ=255)                                                                                        tGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATg                                                                                                                              >  1:622657/1‑251 (MQ=255)                                                                                            cccccccgagctgtgcACCGACATTGATCGGTTACTGGCCTCCAGTGCAGGTATCGCGGTTGAAGTTCGTGAAGCGGTGCAGTTGCTGACGGGTGAGTGTCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTTTGCGTGGTGATGCTTATCTCCGGCAAACTGGCGAAAGAGGACGCCGAAGCGCGCTCGTAATTG‑CAAGCAGTGCTGGACAACGGTATACCGGCAGAAGTCTTGGGTCGTATGGTAg                                                                                                                          <  2:336807/236‑1 (MQ=255)                                                                                            caccacCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAg                                                                                                                          <  1:251017/251‑1 (MQ=255)                                                                                            caccacCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAg                                                                                                                          <  1:549798/251‑1 (MQ=255)                                                                                             cccaccGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑c                                                                                                                        <  1:566719/250‑1 (MQ=255)                                                                                                     gctgctCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGACGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑aa                                                                                                                >  2:546532/1‑250 (MQ=255)                                                                                                     gctgctCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑aaa                                                                                                               <  1:625342/251‑1 (MQ=255)                                                                                                     gctgctCACCGAAATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGCCAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGAAC‑aa                                                                                                                >  1:382488/1‑250 (MQ=255)                                                                                                         ctCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAgg                                                                                                           >  1:171774/1‑251 (MQ=255)                                                                                                           cACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGcc                                                                                                         <  1:588932/251‑1 (MQ=255)                                                                                                             ccGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGccc                                                                                                        >  1:274604/1‑250 (MQ=255)                                                                                                               gACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGGCTTTGGGCGTATGGTAG‑CGGCACCAAAAAGGccg                                                                                                        >  1:275355/1‑248 (MQ=255)                                                                                                               gACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAGGCGGTGCTGGACAACGGGAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCccggg                                                                                                     >  1:47426/1‑249 (MQ=255)                                                                                                               gACATGAATCAGGTACTGGCCGCCAGGGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCccg                                                                                                       <  2:275355/249‑1 (MQ=255)                                                                                                               acaaTGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCccg                                                                                                       <  2:47426/246‑1 (MQ=255)                                                                                                                    gAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACt                                                                                                >  1:480116/1‑251 (MQ=255)                                                                                                                    gAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACt                                                                                                <  1:109937/251‑1 (MQ=255)                                                                                                                         aGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCATCCGACTTCGtt                                                                                           <  1:577704/251‑1 (MQ=255)                                                                                                                             aCTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTgaga                                                                                       <  1:196144/251‑1 (MQ=255)                                                                                                                             aCTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGAAGTTCCTGCCGGGTGAGTATCGTAACCCGCGTCTGTTTGCTGTCACGATGGCGCTGTGCGGGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGAAGCCGAAGCGCGAGAGAAATTGACAG‑CAATGCTGGACACCGGGAAAGCGGCAGAAGTCTTTGGGCGTATGG‑AGACGGCAC‑AAAAAGACCCAACCGAATTCGTTgtg                                                                                        >  2:406726/1‑248 (MQ=255)                                                                                                                              cTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTg                                                                                                                                                                  <  2:303906/177‑1 (MQ=255)                                                                                                                              cTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTg                                                                                                                                                                  >  1:303906/1‑177 (MQ=255)                                                                                                                                      cAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTAcgcg                                                                               >  1:48413/1‑250 (MQ=255)                                                                                                                                       aGTGCAGGTAACGCGGTTGACGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCAGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTACGGCAAACTGGCGAAAGATGACGCCGAAGCGAGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGACAAAAGACTTTGATCGTATGGTAA‑CGGCAC‑AAAAAGGCCCAACAGACTTCGtgtaggactagtcga                                                                              >  2:327934/1‑236 (MQ=255)                                                                                                                                       aGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGa                                                                              >  1:135213/1‑250 (MQ=255)                                                                                                                                         tGCAGGTACCGCGGTTGACGTTAGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGATGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTATTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGAATTAGTTGAGAACTACGCGAAgg                                                                           >  2:320613/1‑250 (MQ=255)                                                                                                                                         tGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGt                                                                           >  1:176690/1‑251 (MQ=255)                                                                                                                                            ccgattgtcgGTTGAAGTTCTGGAAGCGGTGCAGTTCCGGACGGGGGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATc                                                                        <  2:601410/243‑1 (MQ=255)                                                                                                                                                aaCGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCg                                                                                                                                                                                                >  1:676633/1‑130 (MQ=255)                                                                                                                                                aaCGCGGTTGAAGTTCGAGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGcc                                                                    <  1:493174/251‑1 (MQ=255)                                                                                                                                                 aCGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCg                                                                   <  1:608535/251‑1 (MQ=255)                                                                                                                                                   gcgGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATc                                                                        >  1:601410/1‑244 (MQ=255)                                                                                                                                                    cgGTTGAAGTTCGTGAAGCTGGGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCGGTTTGATGTCACGATGGCTCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGTGAAATTG‑CAAGCAGTGCTGGACAACGGTAAATCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACa                                                                <  2:675936/251‑1 (MQ=255)                                                                                                                                                    cgGTTGAAGTTCGTGAAGCGGAGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGACTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGAAGAAGTATTTGGTCGTAAGATAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAATGATCTGCCGAcc                                                                >  2:630675/1‑250 (MQ=255)                                                                                                                                                      gTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGaa                                                                                                                                                                                              <  1:458664/126‑1 (MQ=255)                                                                                                                                                      gTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGaa                                                                                                                                                                                              >  2:458664/1‑126 (MQ=255)                                                                                                                                                               cGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGAc                                                     >  1:665587/1‑251 (MQ=255)                                                                                                                                                               cGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGAc                                                     <  1:204290/251‑1 (MQ=255)                                                                                                                                                                        gTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGt                                            <  1:660192/251‑1 (MQ=255)                                                                                                                                                                        gTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGATTATCGCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCa                                              <  2:204937/249‑1 (MQ=255)                                                                                                                                                                            agttccgtaCGGTTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGGTGGGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGGCTAt                                        <  2:504690/243‑1 (MQ=255)                                                                                                                                                                            aGTTCCTGACGGGAGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGCAGTATCTGCCGACCGCGATGCTGACGAAAGCAGTCTAt                                        >  1:354986/1‑251 (MQ=255)                                                                                                                                                                                   gACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGAt                                  <  1:623405/250‑1 (MQ=255)                                                                                                                                                                                    aCGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATAc                                <  1:546532/251‑1 (MQ=255)                                                                                                                                                                                    aCGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCACCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATAc                                >  2:596344/1‑251 (MQ=255)                                                                                                                                                                                              atcgataCCCGCGTCCGTTTGATGTCCCGATGGCGTTGTGCGTGGAGATGCTTATCTTCGGCAAACTGGCGAAAGATGACGCCGAAGCTCTCTCTAAAGTG‑CAAGCAGTGCTGGCCAACGGTAAAGCGGCATTAGTCTTTGGTCGTATGGTTT‑CGGCAC‑AACAAGGCCCAACCGACTTCGTTGAGATCTACGCGAAGTATCTGCCGACAGCGATGCTGACGAATGCAGCCTATGCTGATACCGAAGTtttt                      <  2:508597/245‑1 (MQ=255)                                                                                                                                                                                                   accccGCGTCTGCTTGAAGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACGGGCGAAAGATGCCGCCGAAGCGCACGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGCACCAACCGACTTCTATGAGAAATAAGCGAAGTATCTGCCGACGGCACTGCTGACGACAGCAGTCTATGCTGCTACCGACGGTTTTGTc                   >  2:122481/3‑249 (MQ=255)                                                                                                                                                                                                   aacccttgTCTGTTTGATGTCACGAGGGCGCTGTGCGTGGAGATGCTTATCTCCTGCAATCTGGCGAAAGATGACGCCGAAGCGCGCGCGCAATTG‑CAAGCAGTGCTGGCCAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGTCTTCGTTTAGAACTACGCGAAGAAACTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCa                  <  2:181289/243‑1 (MQ=255)                                                                                                                                                                                                     cccGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTg               <  1:262608/251‑1 (MQ=255)                                                                                                                                                                                                      ccGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGa              <  1:257248/251‑1 (MQ=255)                                                                                                                                                                                                          gTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTAc                                                                                  >  2:232524/1‑179 (MQ=255)                                                                                                                                                                                                          gTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTAc                                                                                  <  1:232524/179‑1 (MQ=255)                                                                                                                                                                                                                ttGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAGGGTTTTGTCAGTGAAATGGATAcc    >  2:300743/1‑251 (MQ=255)                                                                                                                                                                                                                ttGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATAcc    >  1:252251/1‑251 (MQ=255)                                                                                                                                                                                                                ttGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATAcc    >  1:592256/1‑251 (MQ=255)                                                                                                                                                                                                                  gATGTCACGATGGTGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGATGTATCTGCCGACAGCGATGCTGACGAAAGCAGGCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGAGACCcg  <  2:373146/251‑1 (MQ=255)                                                                                                                                                                                                                     gTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGCCGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGaa                                                                             >  2:650538/1‑173 (MQ=255)                                                                                                                                                                                                                     gTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGaa                                                                             <  1:650538/173‑1 (MQ=255)                                                                                                                                                                                                                                          gagaTGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑c                                                                                                                        <  2:194312/110‑1 (MQ=255)                                                                                                                                                                                                                                          gagaTGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑c                                                                                                                        >  1:194312/1‑110 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                              AAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTG‑CAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAG‑CGGCAC‑AAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCG  >  NC_000913/4618839‑4619298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑