Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,617,574 C→A D84E (GAC→GAA)  deoC → deoxyribose‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,617,5740CA98.0% 159.4 / ‑5.3 51D84E (GAC→GAA) deoCdeoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (29/21);  total (30/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01

GAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGA‑TCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGG‑CAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACT  >  NC_000913/4617334‑4617815
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   
gAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTgg                                                                                                                                                                                                                                           >  1:144480/1‑251 (MQ=255)
   agcaagcaGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGCTTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTgcc                                                                                                                                                                                                                                          >  1:400883/1‑247 (MQ=255)
    gcaagcaGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGc                                                                                                                                                                                                                                          >  2:142028/1‑248 (MQ=255)
          agcCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTACCCCGATTGCTCGCAAAACACTGAAAGAGCAGTGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCg                                                                                                                                                                                                                                 >  1:43902/1‑251 (MQ=255)
                     aCTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGAAGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATcc                                                                                                                                                                                                                      >  1:11926/1‑250 (MQ=255)
                          aaTTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAC‑TTGCTCTGAGTCATCAGGCCAAAACCCCGGTAGGCAATACCGCCGCTATCTGTATCTATCATCGCTTTATCCCGATTGCTAGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTCCGCTAACAAACTTCCAACACGGTAACGATGACATCGAAATAACGCTGGCAGAAACCCGTGCGGCCATCGcatcc                                                                                                                                                                                                                 >  2:174079/1‑247 (MQ=255)
                              gATGGACCCGCCCACCCTAAATGACGACGACACCGACGAAAAAGTAA‑TTGCTTTGTGTCACCAGACCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGt                                                                                                                                                                                                              <  1:299227/250‑1 (MQ=255)
                                 ggACCTGACCACCCTGAATGACGACGACCCCGACGAGAAAGCAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATATGTATCTATCCTCGCTTTATCCCGATTGCACGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCAGCATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAACTCGAGCTGCCAGGAACACTTGCGGCAATCGCCTACGGTGccg                                                                                                                                                                                                          >  1:672464/1‑249 (MQ=255)
                                     cTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCcgatg                                                                                                                                                                                                       >  1:593478/1‑250 (MQ=255)
                                      catctccccTGAATGACGACGACACCGACGA‑AAAATAAGTTGCTCTTGGTCATCAGCCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGaa                                                                                                                                                                                                     <  1:39305/245‑1 (MQ=255)
                                           accCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGaa                                                                                                                                                                                                     >  2:39305/1‑246 (MQ=255)
                                                 aaTGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGtggg                                                                                                                                                                                          >  1:219543/1‑248 (MQ=255)
                                                                   tagaAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGTAAATCCGTATTGCTACGGTAACCAACTTCCAACACGGTAACGATGACCTCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGAAGTGGAGTTCCCGTAACGCGCGCt                                                                                                                                                                        >  2:509213/2‑251 (MQ=255)
                                                                     gaAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTg                                                                                                                                                                       >  1:244064/1‑250 (MQ=255)
                                                                         agtaattgctctgagtCATCAGGCCAAAACCCCGGTCGGCAATACCACCGCTATCTGTCTCTATCCTCGCTTTATCCCGATAGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGCAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGGTGACATCGAAATCGCGCGGGCAGCAACCCGTGCGGCAATCTCCTACGGTGACGATGAAGTATACGTGGTGGTCCCGAACCGCGCGCTGacgg                                                                                                                                                                   >  2:219610/15‑248 (MQ=255)
                                                                               tgctcggtgtCATCAGCCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGt                                                                                                                                                              <  1:281345/244‑1 (MQ=255)
                                                                                gctcTGTGTCACCAGGCCAAAACCCCGGTCGTCAATTCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTa                                                                                                                                                             <  1:255707/247‑1 (MQ=255)
                                                                                   cTGTGACATCAGCCGAAACCCCCGGTAGACAATACCGCCGCTAGCTGTATCTATCCTCGCCTTCTCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCGACATACCACACGGTTACGATGCAATCGAAATCGCGCTGACAGAAACCCGTACGGCAATCGCATTCGGGGCCGATGACGTTGATGTGGTGTtaccgtaccgaccgctaccggcggggtacg                                                                                                                                                          >  2:124547/1‑230 (MQ=255)
                                                                                       gtCATCAGGCCAAAACCCCGGTCGGCAAATCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAg                                                                                                                                                     <  1:630271/251‑1 (MQ=255)
                                                                                       gtCAACAGGCAAAACCCCCGTTCGGCATAACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAg                                                                                                                                                     <  1:224296/251‑1 (MQ=255)
                                                                                          atcaGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGa                                                                                                                                                                      >  1:380394/1‑231 (MQ=255)
                                                                                            cggtccacacccccGGTCGGAAATCCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAggttgg                                                                                                                                                <  2:146221/239‑1 (MQ=255)
                                                                                                    acccccgcggggaaacaCCGCCGCGAGCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GAcc                                                                                                                                       <  2:341916/235‑1 (MQ=255)
                                                                                                              ggCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGc                                                                                                                             >  1:103645/1‑251 (MQ=255)
                                                                                                               gaaaTACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGcc                                                                                                                            <  1:211407/249‑1 (MQ=255)
                                                                                                                     ccgccgCTAACTGTATCTAACCTCGCCTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCGCCCCGTAAATCCGTACTGCTACGTGAACCAACTTCCCACGCGGTAACCATGACATCGAAATCGCGCTGGCAGAAACCCGCGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGAGGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTaaa                                                                                                                      <  2:275221/251‑1 (MQ=255)
                                                                                                                      cgccgcTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGtca                                                                                                                       >  1:557682/1‑247 (MQ=255)
                                                                                                                        ccgcTATCGGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAagag                                                                                                                   <  2:219543/251‑1 (MQ=255)
                                                                                                                                 gTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGTAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGAGCCGATGCAGTTGACGTGGTGTTCACGTCCAGCGCGCTGATGTCGGGAAACGCGCAGGGGGGGTTT‑TACCTGGTGAAAGACTGTCAAGAGGCTTGcgcg                                                                                                          >  2:483050/1‑251 (MQ=255)
                                                                                                                                 gTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGcgcg                                                                                                          <  1:395715/251‑1 (MQ=255)
                                                                                                                                   atctatcCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGGGCAGGTTGGTTTTTGCCCGGGT‑AAAGCCTGAAAAGAgggtttgcgggg                                                                                                        >  2:232742/1‑241 (MQ=255)
                                                                                                                                     ctatcCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGGGAAAGCCTGTAAAGAGGCTTTCGCGG‑c                                                                                                       >  1:483978/1‑249 (MQ=255)
                                                                                                                                             gCTTTTTCCCGATTGCTCGCAAACCACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTAAGGTCACCAAATTCCCACACGGTCACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGAAATCGCCTACGGAGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACCAGCCGGCTGGTTTT‑GACCTGGTGCAAGCCAGTACAGAGGCTTgcgggggcgcggatgt                                                                                               >  2:612311/1‑237 (MQ=255)
                                                                                                                                                  aTCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAACGAGCCGGTTGGGTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGG‑CAGCGAATGTActgct                                                                                        >  1:427582/1‑251 (MQ=255)
                                                                                                                                                              cGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGG‑CAGCGAATGTACTGCTGAAAGTGatca                                                                             <  1:36444/250‑1 (MQ=255)
                                                                                                                                                                cAAAACACTGCAAGCGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAAATTCCCACACGGTAACGATGACATCGTAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGCTGGCGGGAAACGAGCAGGTAGGGTTT‑GACCTGGTGAAAGCCTGTGAAGAGGATTGGGCGG‑CAGGGGATGGACTGCTGCAGATGATCATCg                                                                          >  1:370434/1‑251 (MQ=255)
                                                                                                                                                                cAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGGTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTg                                                                                                              >  1:196722/1‑216 (MQ=255)
                                                                                                                                                                   aacactGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGG‑CAGCGAATGTACTGCTGAAAGTGATCATCGaaa                                                                       <  2:548546/247‑1 (MQ=255)
                                                                                                                                                                   aacactGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGGTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGG‑CAGCGAATGTACTGCTGAAAGAGAGCATCGaa                                                                        >  1:622815/5‑250 (MQ=255)
                                                                                                                                                                         gAAAGAGCAGTGCACCCCGGAAATCCGTAATGCTACGGTAACCAACTCCCCACACGGTAACGATTACATCGAAATCTGGCTGGCGGAACCCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGG‑CAGCGAAGGTACTGCTTAAAGTGATCATCGAAACCGGCg                                                                 <  2:597761/251‑1 (MQ=255)
                                                                                                                                                                         gAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGGTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGG‑CAGCGAATGTACTGCTGAAAGTGATCATCGAAACCgg                                                                   <  1:612311/249‑1 (MQ=255)
                                                                                                                                                                           aaGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGG‑CAGCGGATGTACTGCTGAAAGGGATCATCGAAAACGGCGa                                                                >  1:416419/1‑250 (MQ=255)
                                                                                                                                                                           aaGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTGGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGG‑CAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGaa                                                               <  2:483978/251‑1 (MQ=255)
                                                                                                                                                                            agcgCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAAAGAGCAGGGTGGTTTT‑TACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGG‑CAGCGGATGGACTGCTGAAAGTGATCATCAAAACCGGCGAAc                                                              >  2:294121/4‑251 (MQ=255)
                                                                                                                                                                                             aaaTCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGG‑CAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTg                                             <  2:244064/251‑1 (MQ=255)
                                                                                                                                                                                                            cGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGGGAAAGCCTGTAAAGAGGCTTGCGCGG‑CAGGGGATGTAATGCTGAAAGTGATCATCGAAACCGGCGCACCGAAAAAGGAAGCGCTGagcccgaaagaggat                              >  2:312757/1‑237 (MQ=255)
                                                                                                                                                                                                             ggTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTAACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGCGCACGTTGTGTTT‑GACCTGGt                                                                                                                                   >  1:232235/1‑150 (MQ=255)
                                                                                                                                                                                                                    aaCTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGG‑CAGCGAATGTACTGCTGAAAGTGATCATCCAAACCGGCGAACTTGAAGACGAAGCGCGGATCCGTAAAGCGTCTGaaaattc                      >  1:296277/1‑247 (MQ=255)
                                                                                                                                                                                                                             cacGGTAATGATGACATCGAAATCGCGCTGGCAGAAACCAGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGG‑CAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGc             <  1:500037/251‑1 (MQ=255)
                                                                                                                                                                                                                              aaggTAACGATGACATCGAAATCGCGTTGGCAGAATCCCGTGCGGAAATCGCCTACGGTGCCGATGAAGGTGACGTGGTGTGCCCGTACCGCGCGCTGAGGGAGGGTAACGAGCAGGTAGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGG‑CAGCGAATGTACTGCTGAAAGTGAGCAACGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGGCTGAAATCTCCAGCAAAgag            <  2:43902/249‑3 (MQ=255)
                                                                                                                                                                                                                                   aaCGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGG‑CAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGc       <  1:116409/251‑1 (MQ=255)
                                                                                                                                                                                                                                      gatgacatcgaaatcGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGCTGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAAAGACTTGAGCGGACGGGGAAT‑TACTTCTGAAAGGGATGATCGAAACCGGCGAACTGACAGGCGCAGAGCCGAGCCGGAAAACGGCTGAAATCTCCATCCAAGACGGTGCggg    >  2:578196/4‑250 (MQ=255)
                                                                                                                                                                                                                                       atgacatcgaaatcGCGCTGGCAGAAGCCCGGGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGG‑CAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGAc   <  2:367427/249‑1 (MQ=255)
                                                                                                                                                                                                                                        tgacatcgaaatcGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGG‑CAGCGAATGTAATGCTGAAAGGGGTCATCGAAACCGGCGAACTGAAAGACGCAGCGCTGGTCCGTAAAGCGTCTGAAATCTCCATCCAAACGGGGGCgggct  >  1:597892/2‑248 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   
GAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGA‑TCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGG‑CAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACT  >  NC_000913/4617334‑4617815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑