Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,617,532	C→T	I70I (ATC→ATT)	deoC →	deoxyribose‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,617,532	0	C	T	100.0%	161.1 / NA	50	I70I (ATC→ATT)	deoC	deoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/18);  total (32/18)

CAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGA‑TCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTG  >  NC_000913/4617311‑4617772                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  cAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTa                                                                                                                                                                                                                       >  2:281345/1‑250 (MQ=255)         tgaaatgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACgatgac                                                                                                                                                                                                               >  1:341916/1‑250 (MQ=255)            aatgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCACCTTCCCACACGGTAACGAtgacatcg                                                                                                                                                                                                           >  2:255707/1‑251 (MQ=255)             atgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGGATTGCTACGGAAACCAACTTCCCACACGGTAACGAtgccatcg                                                                                                                                                                                                           >  2:395715/1‑250 (MQ=255)                        gAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTgg                                                                                                                                                                                               >  1:144480/1‑251 (MQ=255)                           agcaagcaGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGCTTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTgcc                                                                                                                                                                                              >  1:400883/1‑247 (MQ=255)                            gcaagcaGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGc                                                                                                                                                                                              >  2:142028/1‑248 (MQ=255)                                 cagcCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCtgc                                                                                                                                                                                                                                              >  1:164162/1‑193 (MQ=255)                                  agcCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTACCCCGATTGCTCGCAAAACACTGAAAGAGCAGTGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCg                                                                                                                                                                                     >  1:43902/1‑251 (MQ=255)                                             aCTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGAAGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATcc                                                                                                                                                                          >  1:11926/1‑250 (MQ=255)                                                  aaTTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAC‑TTGCTCTGAGTCATCAGGCCAAAACCCCGGTAGGCAATACCGCCGCTATCTGTATCTATCATCGCTTTATCCCGATTGCTAGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTCCGCTAACAAACTTCCAACACGGTAACGATGACATCGAAATAACGCTGGCAGAAACCCGTGCGGCCATCGcatcc                                                                                                                                                                     >  2:174079/1‑247 (MQ=255)                                                      gATGGACCCGCCCACCCTAAATGACGACGACACCGACGAAAAAGTAA‑TTGCTTTGTGTCACCAGACCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGt                                                                                                                                                                  <  1:299227/250‑1 (MQ=255)                                                         ggACCTGACCACCCTGAATGACGACGACCCCGACGAGAAAGCAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATATGTATCTATCCTCGCTTTATCCCGATTGCACGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCAGCATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAACTCGAGCTGCCAGGAACACTTGCGGCAATCGCCTACGGTGccg                                                                                                                                                              >  1:672464/1‑249 (MQ=255)                                                             cTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCcgatg                                                                                                                                                           >  1:593478/1‑250 (MQ=255)                                                              catctccccTGAATGACGACGACACCGACGA‑AAAATAAGTTGCTCTTGGTCATCAGCCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGaa                                                                                                                                                         <  1:39305/245‑1 (MQ=255)                                                                   accCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGaa                                                                                                                                                         >  2:39305/1‑246 (MQ=255)                                                                         aaTGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGtggg                                                                                                                                              >  1:219543/1‑248 (MQ=255)                                                                                           tagaAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGTAAATCCGTATTGCTACGGTAACCAACTTCCAACACGGTAACGATGACCTCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGAAGTGGAGTTCCCGTAACGCGCGCt                                                                                                                            >  2:509213/2‑251 (MQ=255)                                                                                             gaAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTg                                                                                                                           >  1:244064/1‑250 (MQ=255)                                                                                                 agtaattgctctgagtCATCAGGCCAAAACCCCGGTCGGCAATACCACCGCTATCTGTCTCTATCCTCGCTTTATCCCGATAGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGCAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGGTGACATCGAAATCGCGCGGGCAGCAACCCGTGCGGCAATCTCCTACGGTGACGATGAAGTATACGTGGTGGTCCCGAACCGCGCGCTGacgg                                                                                                                       >  2:219610/15‑248 (MQ=255)                                                                                                       tgctcggtgtCATCAGCCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGt                                                                                                                  <  1:281345/244‑1 (MQ=255)                                                                                                        gctcTGTGTCACCAGGCCAAAACCCCGGTCGTCAATTCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTa                                                                                                                 <  1:255707/247‑1 (MQ=255)                                                                                                           cTGTGACATCAGCCGAAACCCCCGGTAGACAATACCGCCGCTAGCTGTATCTATCCTCGCCTTCTCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCGACATACCACACGGTTACGATGCAATCGAAATCGCGCTGACAGAAACCCGTACGGCAATCGCATTCGGGGCCGATGACGTTGATGTGGTGTtaccgtaccgaccgctaccggcggggtacg                                                                                                              >  2:124547/1‑230 (MQ=255)                                                                                                               gtCATCAGGCCAAAACCCCGGTCGGCAAATCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAg                                                                                                         <  1:630271/251‑1 (MQ=255)                                                                                                               gtCAACAGGCAAAACCCCCGTTCGGCATAACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAg                                                                                                         <  1:224296/251‑1 (MQ=255)                                                                                                                  atcaGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGa                                                                                                                          >  1:380394/1‑231 (MQ=255)                                                                                                                    cggtccacacccccGGTCGGAAATCCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAggttgg                                                                                                    <  2:146221/239‑1 (MQ=255)                                                                                                                            acccccgcggggaaacaCCGCCGCGAGCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GAcc                                                                                           <  2:341916/235‑1 (MQ=255)                                                                                                                                      ggCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGc                                                                                 >  1:103645/1‑251 (MQ=255)                                                                                                                                       gaaaTACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGcc                                                                                <  1:211407/249‑1 (MQ=255)                                                                                                                                             ccgccgCTAACTGTATCTAACCTCGCCTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCGCCCCGTAAATCCGTACTGCTACGTGAACCAACTTCCCACGCGGTAACCATGACATCGAAATCGCGCTGGCAGAAACCCGCGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGAGGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTaaa                                                                          <  2:275221/251‑1 (MQ=255)                                                                                                                                              cgccgcTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGtca                                                                           >  1:557682/1‑247 (MQ=255)                                                                                                                                                ccgcTATCGGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAagag                                                                       <  2:219543/251‑1 (MQ=255)                                                                                                                                                         gTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGTAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGAGCCGATGCAGTTGACGTGGTGTTCACGTCCAGCGCGCTGATGTCGGGAAACGCGCAGGGGGGGTTT‑TACCTGGTGAAAGACTGTCAAGAGGCTTGcgcg                                                              >  2:483050/1‑251 (MQ=255)                                                                                                                                                         gTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGcgcg                                                              <  1:395715/251‑1 (MQ=255)                                                                                                                                                           atctatcCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGGGCAGGTTGGTTTTTGCCCGGGT‑AAAGCCTGAAAAGAgggtttgcgggg                                                            >  2:232742/1‑241 (MQ=255)                                                                                                                                                             ctatcCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGGGAAAGCCTGTAAAGAGGCTTTCGCGGc                                                            >  1:483978/1‑249 (MQ=255)                                                                                                                                                                     gCTTTTTCCCGATTGCTCGCAAACCACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTAAGGTCACCAAATTCCCACACGGTCACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGAAATCGCCTACGGAGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACCAGCCGGCTGGTTTT‑GACCTGGTGCAAGCCAGTACAGAGGCTTgcgggggcgcggatgt                                                   >  2:612311/1‑237 (MQ=255)                                                                                                                                                                          aTCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAACGAGCCGGTTGGGTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTActgct                                             >  1:427582/1‑251 (MQ=255)                                                                                                                                                                                      cGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGatca                                  <  1:36444/250‑1 (MQ=255)                                                                                                                                                                                        cAAAACACTGCAAGCGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAAATTCCCACACGGTAACGATGACATCGTAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGCTGGCGGGAAACGAGCAGGTAGGGTTT‑GACCTGGTGAAAGCCTGTGAAGAGGATTGGGCGGCAGGGGATGGACTGCTGCAGATGATCATCg                               >  1:370434/1‑251 (MQ=255)                                                                                                                                                                                        cAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGGTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTg                                                                  >  1:196722/1‑216 (MQ=255)                                                                                                                                                                                           aacactGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGaaa                            <  2:548546/247‑1 (MQ=255)                                                                                                                                                                                           aacactGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGGTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGAGAGCATCGaa                             >  1:622815/5‑250 (MQ=255)                                                                                                                                                                                                 gAAAGAGCAGTGCACCCCGGAAATCCGTAATGCTACGGTAACCAACTCCCCACACGGTAACGATTACATCGAAATCTGGCTGGCGGAACCCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAAGGTACTGCTTAAAGTGATCATCGAAACCGGCg                      <  2:597761/251‑1 (MQ=255)                                                                                                                                                                                                 gAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGGTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCgg                        <  1:612311/249‑1 (MQ=255)                                                                                                                                                                                                   aaGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGGATGTACTGCTGAAAGGGATCATCGAAAACGGCGa                     >  1:416419/1‑250 (MQ=255)                                                                                                                                                                                                   aaGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTGGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGaa                    <  2:483978/251‑1 (MQ=255)                                                                                                                                                                                                    agcgCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAAAGAGCAGGGTGGTTTT‑TACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGGATGGACTGCTGAAAGTGATCATCAAAACCGGCGAAc                   >  2:294121/4‑251 (MQ=255)                                                                                                                                                                                                                     aaaTCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTg  <  2:244064/251‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  CAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGA‑TCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTG  >  NC_000913/4617311‑4617772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑