Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,617,499	T→A	T59T (ACT→ACA)	deoC →	deoxyribose‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,617,499	0	T	A	100.0%	137.1 / NA	44	T59T (ACT→ACA)	deoC	deoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (30/14);  total (30/14)

AATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGA‑TCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCG  >  NC_000913/4617269‑4617743                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      aatcaaaacaaccgctaaaGGTAAATTTTATTGGCGAAACGCAAGGAAAATGAAAGGACTGATTTGAAAGTAAGCAGCCTGCTTACATTGAAATTGAGGGCCGTGACCACCCTGAATGACACCGACACCGACGAAAAATTAA‑TTGCTTTGTGTCACCATCCCAAACCCCCGGTCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCAcccc                                                                                                                                                                                                                                   <  1:386647/234‑1 (MQ=255)          acaCTCGCACGGTGACTTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGCTCTGAAAGAAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTAGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCg                                                                                                                                                                                                                          >  2:635825/3‑251 (MQ=255)                                           cAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTa                                                                                                                                                                                          >  2:281345/1‑250 (MQ=255)                                                   tgaaatgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACgatgac                                                                                                                                                                                  >  1:341916/1‑250 (MQ=255)                                                      aatgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCACCTTCCCACACGGTAACGAtgacatcg                                                                                                                                                                              >  2:255707/1‑251 (MQ=255)                                                       atgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGGATTGCTACGGAAACCAACTTCCCACACGGTAACGAtgccatcg                                                                                                                                                                              >  2:395715/1‑250 (MQ=255)                                                                  gAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTgg                                                                                                                                                                  >  1:144480/1‑251 (MQ=255)                                                                     agcaagcaGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGCTTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTgcc                                                                                                                                                                 >  1:400883/1‑247 (MQ=255)                                                                      gcaagcaGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGc                                                                                                                                                                 >  2:142028/1‑248 (MQ=255)                                                                           cagcCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCtgc                                                                                                                                                                                                                 >  1:164162/1‑193 (MQ=255)                                                                            agcCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTACCCCGATTGCTCGCAAAACACTGAAAGAGCAGTGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCg                                                                                                                                                        >  1:43902/1‑251 (MQ=255)                                                                                       aCTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGAAGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATcc                                                                                                                                             >  1:11926/1‑250 (MQ=255)                                                                                            aaTTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAC‑TTGCTCTGAGTCATCAGGCCAAAACCCCGGTAGGCAATACCGCCGCTATCTGTATCTATCATCGCTTTATCCCGATTGCTAGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTCCGCTAACAAACTTCCAACACGGTAACGATGACATCGAAATAACGCTGGCAGAAACCCGTGCGGCCATCGcatcc                                                                                                                                        >  2:174079/1‑247 (MQ=255)                                                                                                gATGGACCCGCCCACCCTAAATGACGACGACACCGACGAAAAAGTAA‑TTGCTTTGTGTCACCAGACCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGt                                                                                                                                     <  1:299227/250‑1 (MQ=255)                                                                                                   ggACCTGACCACCCTGAATGACGACGACCCCGACGAGAAAGCAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATATGTATCTATCCTCGCTTTATCCCGATTGCACGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCAGCATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAACTCGAGCTGCCAGGAACACTTGCGGCAATCGCCTACGGTGccg                                                                                                                                 >  1:672464/1‑249 (MQ=255)                                                                                                       cTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCcgatg                                                                                                                              >  1:593478/1‑250 (MQ=255)                                                                                                        catctccccTGAATGACGACGACACCGACGA‑AAAATAAGTTGCTCTTGGTCATCAGCCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGaa                                                                                                                            <  1:39305/245‑1 (MQ=255)                                                                                                             accCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGaa                                                                                                                            >  2:39305/1‑246 (MQ=255)                                                                                                                   aaTGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGtggg                                                                                                                 >  1:219543/1‑248 (MQ=255)                                                                                                                                     tagaAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGTAAATCCGTATTGCTACGGTAACCAACTTCCAACACGGTAACGATGACCTCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGAAGTGGAGTTCCCGTAACGCGCGCt                                                                                               >  2:509213/2‑251 (MQ=255)                                                                                                                                       gaAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTg                                                                                              >  1:244064/1‑250 (MQ=255)                                                                                                                                           agtaattgctctgagtCATCAGGCCAAAACCCCGGTCGGCAATACCACCGCTATCTGTCTCTATCCTCGCTTTATCCCGATAGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGCAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGGTGACATCGAAATCGCGCGGGCAGCAACCCGTGCGGCAATCTCCTACGGTGACGATGAAGTATACGTGGTGGTCCCGAACCGCGCGCTGacgg                                                                                          >  2:219610/15‑248 (MQ=255)                                                                                                                                                 tgctcggtgtCATCAGCCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGt                                                                                     <  1:281345/244‑1 (MQ=255)                                                                                                                                                  gctcTGTGTCACCAGGCCAAAACCCCGGTCGTCAATTCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTa                                                                                    <  1:255707/247‑1 (MQ=255)                                                                                                                                                     cTGTGACATCAGCCGAAACCCCCGGTAGACAATACCGCCGCTAGCTGTATCTATCCTCGCCTTCTCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCGACATACCACACGGTTACGATGCAATCGAAATCGCGCTGACAGAAACCCGTACGGCAATCGCATTCGGGGCCGATGACGTTGATGTGGTGTtaccgtaccgaccgctaccggcggggtacg                                                                                 >  2:124547/1‑230 (MQ=255)                                                                                                                                                         gtCATCAGGCCAAAACCCCGGTCGGCAAATCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAg                                                                            <  1:630271/251‑1 (MQ=255)                                                                                                                                                         gtCAACAGGCAAAACCCCCGTTCGGCATAACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAg                                                                            <  1:224296/251‑1 (MQ=255)                                                                                                                                                            atcaGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGa                                                                                             >  1:380394/1‑231 (MQ=255)                                                                                                                                                              cggtccacacccccGGTCGGAAATCCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAggttgg                                                                       <  2:146221/239‑1 (MQ=255)                                                                                                                                                                      acccccgcggggaaacaCCGCCGCGAGCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GAcc                                                              <  2:341916/235‑1 (MQ=255)                                                                                                                                                                                ggCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGc                                                    >  1:103645/1‑251 (MQ=255)                                                                                                                                                                                 gaaaTACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGcc                                                   <  1:211407/249‑1 (MQ=255)                                                                                                                                                                                       ccgccgCTAACTGTATCTAACCTCGCCTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCGCCCCGTAAATCCGTACTGCTACGTGAACCAACTTCCCACGCGGTAACCATGACATCGAAATCGCGCTGGCAGAAACCCGCGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGAGGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTaaa                                             <  2:275221/251‑1 (MQ=255)                                                                                                                                                                                        cgccgcTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGtca                                              >  1:557682/1‑247 (MQ=255)                                                                                                                                                                                          ccgcTATCGGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAagag                                          <  2:219543/251‑1 (MQ=255)                                                                                                                                                                                                   gTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGTAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGAGCCGATGCAGTTGACGTGGTGTTCACGTCCAGCGCGCTGATGTCGGGAAACGCGCAGGGGGGGTTT‑TACCTGGTGAAAGACTGTCAAGAGGCTTGcgcg                                 >  2:483050/1‑251 (MQ=255)                                                                                                                                                                                                   gTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGcgcg                                 <  1:395715/251‑1 (MQ=255)                                                                                                                                                                                                     atctatcCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGGGCAGGTTGGTTTTTGCCCGGGT‑AAAGCCTGAAAAGAgggtttgcgggg                               >  2:232742/1‑241 (MQ=255)                                                                                                                                                                                                       ctatcCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGGGAAAGCCTGTAAAGAGGCTTTCGCGGc                               >  1:483978/1‑249 (MQ=255)                                                                                                                                                                                                               gCTTTTTCCCGATTGCTCGCAAACCACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTAAGGTCACCAAATTCCCACACGGTCACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGAAATCGCCTACGGAGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACCAGCCGGCTGGTTTT‑GACCTGGTGCAAGCCAGTACAGAGGCTTgcgggggcgcggatgt                      >  2:612311/1‑237 (MQ=255)                                                                                                                                                                                                                    aTCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAACGAGCCGGTTGGGTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTActgct                >  1:427582/1‑251 (MQ=255)                                                                                                                                                                                                                                cGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGatca     <  1:36444/250‑1 (MQ=255)                                                                                                                                                                                                                                  cAAAACACTGCAAGCGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAAATTCCCACACGGTAACGATGACATCGTAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGCTGGCGGGAAACGAGCAGGTAGGGTTT‑GACCTGGTGAAAGCCTGTGAAGAGGATTGGGCGGCAGGGGATGGACTGCTGCAGATGATCATCg  >  1:370434/1‑251 (MQ=255)                                                                                                                                                                                                                                  cAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGGTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTg                                     >  1:196722/1‑216 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      AATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGA‑TCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTT‑GACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCG  >  NC_000913/4617269‑4617743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑