Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,617,436	T→C	T38T (ACT→ACC)	deoC →	deoxyribose‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,617,436	0	T	C	100.0%	132.1 / NA	44	T38T (ACT→ACC)	deoC	deoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/12);  total (32/12)

ACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTGTATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGA‑TCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAG  >  NC_000913/4617195‑4617670                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           aCAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGc                                                                                                                                                                                                                                   >  1:548546/1‑251 (MQ=255)                 gTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCt                                                                                                                                                                                                                   >  2:36444/1‑251 (MQ=255)                             ccTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGCCGAGAAAGTAA‑TTGCTCTGGGTCATCAGGCCAAAACCCCGGTCGCCAATACCGCAGCTATATGTATCTATCCTc                                                                                                                                                                                                       >  2:604532/1‑251 (MQ=255)                                 aaTTGTTATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGAAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCGGCGTGCACTGAAATTGATGGACCTGACCCCCCTGAATGACGACGACACCGACGAGAAATTAA‑TTGCCCTGTGTCATCAGGCCAAAACCCCGGTCGTCAATACCGCCGCTATCTGTATCTATCCTCGCt                                                                                                                                                                                                    <  1:330293/250‑1 (MQ=255)                                     gtgATGTAAATCGAAGTGTGTGGCGGAGTAGATGTTAGAATACTAACAACCTCGAAAGGTAAATTTTATTGGCGACAAGCCAGGAGAATGAAAGGACTGTTCTAAAAGCAAGCAGCCGGCTTGCACTGAAATGGAGGGCCCTGACCCCCCGGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCGGGCAAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATc                                                                                                                                                                                               <  1:621642/251‑1 (MQ=255)                                      tgATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGCAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATcc                                                                                                                                                                                              >  1:53973/1‑251 (MQ=255)                                      tgATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATcc                                                                                                                                                                                              >  1:504358/1‑251 (MQ=255)                                            atATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTg                                                                                                                                                                                        >  1:367427/2‑251 (MQ=255)                                            atATCAAAGTGTGGTGGGGATGAGATGTTAGAATCCTAACAAACGCGCAAGGGTATTTTTATTGGCGACAAGCCAGGAGAATGAAATGATTACTCTGAAACCAAGCACCCTGCGTGCACTGAAATTGTTGGACCTGACCACCCTGAATGACAACGACCCCGACAAGAAAGTAT‑TTGCTCGGTTTCATCAGGCCAAACCCCCGGTCGGCAATTCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGAtt                                                                                                                                                                                         <  1:378102/249‑1 (MQ=255)                                              aTCGAAATGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAATCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGCAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTCTCTGTATCTATCCTCGCTTTATCCCGATTGCt                                                                                                                                                                                      >  2:211407/1‑251 (MQ=255)                                                 gAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGc                                                                                                                                                                                   >  1:146221/1‑251 (MQ=255)                                                      gtgtTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGCCGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTCACTATcc                                                                                                                                                                                                         >  2:215847/1‑224 (MQ=255)                                                      gtgtTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAAc                                                                                                                                                                              >  1:284801/1‑251 (MQ=255)                                                      gtgtTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATGGGCGAAAAGCCGGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGAGGGACCTGACCCCCCTGAATGACGACGACCCCGACGAGAAAGTAT‑TTGCTCTGTGTCATCAGGCCAAACCCCCGGGCGGCAATACCGCCGCTATCTGTATCTATcc                                                                                                                                                                                                         <  1:215847/224‑1 (MQ=255)                                                      gtgtTGCGGAGAAGATGTTAGAATACTAACAAACTCGCAAGGGGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACAGAAATTGATGGACCTGAACACCCTGAATGACGACGACACCGACGAGGAAGTAA‑TTGCTCTGTGTCATCCGGCCAACACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTACCCCGATTGCTCGCaaaa                                                                                                                                                                               >  1:333255/1‑250 (MQ=255)                                                          tggggagaggATGTTAAAATACTAAAAACCCCGAAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAAGGACTGATCTAAAAGCAAGAAGCCTGCGTGCACTAAAATTGATGGACCTGACCCCCCTAAATGACGACGACACCGAGGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGCCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAAcact                                                                                                                                                                           <  1:602927/241‑4 (MQ=255)                                                                           aatcaaaacaaccgctaaaGGTAAATTTTATTGGCGAAACGCAAGGAAAATGAAAGGACTGATTTGAAAGTAAGCAGCCTGCTTACATTGAAATTGAGGGCCGTGACCACCCTGAATGACACCGACACCGACGAAAAATTAA‑TTGCTTTGTGTCACCATCCCAAACCCCCGGTCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCAcccc                                                                                                                                                         <  1:386647/234‑1 (MQ=255)                                                                                    acaCTCGCACGGTGACTTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGCTCTGAAAGAAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTAGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCg                                                                                                                                                >  2:635825/3‑251 (MQ=255)                                                                                                                     cAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTa                                                                                                                >  2:281345/1‑250 (MQ=255)                                                                                                                             tgaaatgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACgatgac                                                                                                        >  1:341916/1‑250 (MQ=255)                                                                                                                                aatgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCACCTTCCCACACGGTAACGAtgacatcg                                                                                                    >  2:255707/1‑251 (MQ=255)                                                                                                                                 atgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGGATTGCTACGGAAACCAACTTCCCACACGGTAACGAtgccatcg                                                                                                    >  2:395715/1‑250 (MQ=255)                                                                                                                                            gAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTgg                                                                                        >  1:144480/1‑251 (MQ=255)                                                                                                                                               agcaagcaGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGCTTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTgcc                                                                                       >  1:400883/1‑247 (MQ=255)                                                                                                                                                gcaagcaGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGc                                                                                       >  2:142028/1‑248 (MQ=255)                                                                                                                                                     cagcCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCtgc                                                                                                                                       >  1:164162/1‑193 (MQ=255)                                                                                                                                                      agcCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTACCCCGATTGCTCGCAAAACACTGAAAGAGCAGTGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCg                                                                              >  1:43902/1‑251 (MQ=255)                                                                                                                                                                 aCTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGAAGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATcc                                                                   >  1:11926/1‑250 (MQ=255)                                                                                                                                                                      aaTTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAC‑TTGCTCTGAGTCATCAGGCCAAAACCCCGGTAGGCAATACCGCCGCTATCTGTATCTATCATCGCTTTATCCCGATTGCTAGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTCCGCTAACAAACTTCCAACACGGTAACGATGACATCGAAATAACGCTGGCAGAAACCCGTGCGGCCATCGcatcc                                                              >  2:174079/1‑247 (MQ=255)                                                                                                                                                                          gATGGACCCGCCCACCCTAAATGACGACGACACCGACGAAAAAGTAA‑TTGCTTTGTGTCACCAGACCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGt                                                           <  1:299227/250‑1 (MQ=255)                                                                                                                                                                             ggACCTGACCACCCTGAATGACGACGACCCCGACGAGAAAGCAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATATGTATCTATCCTCGCTTTATCCCGATTGCACGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCAGCATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAACTCGAGCTGCCAGGAACACTTGCGGCAATCGCCTACGGTGccg                                                       >  1:672464/1‑249 (MQ=255)                                                                                                                                                                                 cTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCcgatg                                                    >  1:593478/1‑250 (MQ=255)                                                                                                                                                                                  catctccccTGAATGACGACGACACCGACGA‑AAAATAAGTTGCTCTTGGTCATCAGCCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGaa                                                  <  1:39305/245‑1 (MQ=255)                                                                                                                                                                                       accCTGAATGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGaa                                                  >  2:39305/1‑246 (MQ=255)                                                                                                                                                                                             aaTGACGACGACACCGACGAGAAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGtggg                                       >  1:219543/1‑248 (MQ=255)                                                                                                                                                                                                               tagaAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGTAAATCCGTATTGCTACGGTAACCAACTTCCAACACGGTAACGATGACCTCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGAAGTGGAGTTCCCGTAACGCGCGCt                     >  2:509213/2‑251 (MQ=255)                                                                                                                                                                                                                 gaAAGTAA‑TTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTg                    >  1:244064/1‑250 (MQ=255)                                                                                                                                                                                                                     agtaattgctctgagtCATCAGGCCAAAACCCCGGTCGGCAATACCACCGCTATCTGTCTCTATCCTCGCTTTATCCCGATAGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGCAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGGTGACATCGAAATCGCGCGGGCAGCAACCCGTGCGGCAATCTCCTACGGTGACGATGAAGTATACGTGGTGGTCCCGAACCGCGCGCTGacgg                >  2:219610/15‑248 (MQ=255)                                                                                                                                                                                                                           tgctcggtgtCATCAGCCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGt           <  1:281345/244‑1 (MQ=255)                                                                                                                                                                                                                            gctcTGTGTCACCAGGCCAAAACCCCGGTCGTCAATTCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTa          <  1:255707/247‑1 (MQ=255)                                                                                                                                                                                                                               cTGTGACATCAGCCGAAACCCCCGGTAGACAATACCGCCGCTAGCTGTATCTATCCTCGCCTTCTCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCGACATACCACACGGTTACGATGCAATCGAAATCGCGCTGACAGAAACCCGTACGGCAATCGCATTCGGGGCCGATGACGTTGATGTGGTGTtaccgtaccgaccgctaccggcggggtacg       >  2:124547/1‑230 (MQ=255)                                                                                                                                                                                                                                   gtCATCAGGCCAAAACCCCGGTCGGCAAATCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAg  <  1:630271/251‑1 (MQ=255)                                                                                                                                                                                                                                   gtCAACAGGCAAAACCCCCGTTCGGCATAACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAg  <  1:224296/251‑1 (MQ=255)                                                                                                                                                                                                                                      atcaGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGa                   >  1:380394/1‑231 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           ACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTGTATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGA‑TCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAG  >  NC_000913/4617195‑4617670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑