Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,567,272 C→A intergenic (‑26/+15) yjiN ← / ← mdtM DUF445 domain‑containing protein YjiN/multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,567,2720CA97.7% 124.5 / NA 43intergenic (‑26/+15)yjiN/mdtMDUF445 domain‑containing protein YjiN/multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (31/11);  minor base G (0/1);  total (31/12)
Fisher's exact test for biased strand distribution p-value = 2.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGTGAAAA‑CGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCGACCGAGACCGACATCACCATCAGGATCACCATATTTAGCGATGCGGAGACGGTCCCTTTCGGTAACTTAT  >  NC_000913/4567028‑4567507
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             
aTGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAA‑TTGGGCGTCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCCCGTCTGAGTTCGGTGAGTTTATTCATAACGTTATGAATAGAGCCTGACGGCAGGATcg                                                                                                                                                                                                                                         >  2:188520/1‑251 (MQ=255)
                ggAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCTATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGct                                                                                                                                                                                                                         >  2:14650/1‑251 (MQ=255)
                       gCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCAc                                                                                                                                                                                                                  <  1:590022/251‑1 (MQ=255)
                       gCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCAc                                                                                                                                                                                                                  >  1:254033/1‑251 (MQ=255)
                        cACGCGGCGAAACATCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACt                                                                                                                                                                                                                 <  1:585890/251‑1 (MQ=255)
                        cACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCAAACCTTTAGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAACCACTGCCCCTCCACt                                                                                                                                                                                                                 >  2:577101/1‑251 (MQ=255)
                              gcgAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCg                                                                                                                                                                                                           <  1:369740/251‑1 (MQ=255)
                               cgAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCgg                                                                                                                                                                                                          <  1:14650/251‑1 (MQ=255)
                                     aGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCt                                                                                                                                                                                                    >  1:604291/1‑251 (MQ=255)
                                              accGCAAACCAGTCCGCCAGCGCGCGGACCATCGCCGCTTCAGCAATCGCCTTAACGCCGCTCTCCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTTGGCTGCCTGATGCTGgc                                                                                                                                                                                           <  1:459123/251‑1 (MQ=255)
                                                           ccGCCAGCGCGCCGCCCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGCTGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTg                                                                                                                                                                               >  2:288742/1‑249 (MQ=255)
                                                             gCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGc                                                                                                                                                                            >  1:93754/1‑250 (MQ=255)
                                                              ccAGCGCGCCTACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAA‑TTGGGCGGAAAAAAAAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGTGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTAGTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCaa                                                                                                                                                                          <  2:254033/251‑1 (MQ=255)
                                                                  cgctcctaCCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAA‑TGGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAATccc                                                                                                                                                                      <  2:629570/244‑1 (MQ=255)
                                                                  cgcgcCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAATccc                                                                                                                                                                      >  1:667225/1‑251 (MQ=255)
                                                                     gcCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGCCGGCAGGACCGCGCCAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGCTGCTGGCGCAC‑GCGATTGAGCAATCCCGcc                                                                                                                                                                   >  2:256182/1‑251 (MQ=255)
                                                                        gACCATCGCCGCTTCAGCAATCGCCTTCACGCCGATCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATAACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGC‑CACCGCGATTGGGCAATACCGCCAgg                                                                                                                                                                >  2:601060/1‑251 (MQ=255)
                                                                           cATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGGAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGCTGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGAGCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGCTGCTGGCGCCC‑GCGATTGCGCAATCCCGCCAGGGTg                                                                                                                                                             >  2:163322/1‑251 (MQ=255)
                                                                                    ttCAGCAATCGCCTTCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGTGAAAa‑cgacg                                                                                                                                                   >  1:8545/1‑251 (MQ=255)
                                                                                       aGCAATCGCCTTCACGCCGCTCACCCAAAAA‑GTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCGGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGTGAAAA‑CGACGATa                                                                                                                                                <  1:569736/251‑1 (MQ=255)
                                                                                         cAATCGCCTTCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGTGAAAA‑CGACGATAAc                                                                                                                                              <  1:583206/251‑1 (MQ=255)
                                                                                             cGCCTTCCCGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGGGAAAA‑CGACGATAAcgccc                                                                                                                                          >  2:671241/1‑251 (MQ=255)
                                                                                                 ttCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGGTTGAGCAAt                                                                                                                                                                         >  2:481175/1‑217 (MQ=255)
                                                                                                 ttCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAAt                                                                                                                                                                         <  1:481175/217‑1 (MQ=255)
                                                                                                  tCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGACGATAAGCAGTAAAGAGAGCGCCAACCTTTTGACGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTTACGGCAGGATCGCACAAAAATCCCTGCTACTCCACTAGCTCGGCTGCCTGAAGCTGGCGCAC‑GCGATTCAGCAATCCCGCCAGGGTGACAA‑CGACGGTaaaccccgaccc                                                                                                                                     >  1:471561/1‑241 (MQ=255)
                                                                                                  ccACGCCGC‑CACCCAAAAAATTGGGCGGCAAAAACAGCGAACCGAAAAAGGTAGCAGCGGCGATAAGCAGAAAAGAGGGCGCCAACCTTTTGGCGCGTCTGATTTCGATGAGTTTATTCAAAACGTTAAAAATAGAGCCGGACGGCGGGATCGCGAAAAAATCACTGCCCCCCCCCTAGCGGGGTTGCCTGATGCTGGCGCAC‑GCGATTGAGAAACCCCGCCAGGGTGAAAA‑CGACGATAACGCCCGCcac                                                                                                                                     <  1:360507/250‑1 (MQ=255)
                                                                                                                   aaa‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAATAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAATAATCACTGCTCCTCCACTAGCTCGGCTGCCTGCTGCTGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGCGAAAA‑CGACGCTAACGCCCGCCACAACCGCTTACAGCTGa                                                                                                                     >  1:312830/1‑250 (MQ=255)
                                                                                                                       ttGGGCGGCAAAAACAGCGTAACGACGAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGTGAAAA‑CGACGATAACGCCCGCCACAACGGCTAACAGATGAAACg                                                                                                                 >  1:89081/1‑251 (MQ=255)
                                                                                                                        tGGGCGGAAAAAACAGCGAAACGAAAAAGGGAGCAGCGGGTAAAAAAAAAAAAGGGACCCCCAACTTTTGGGCGCGTCTGATTTCGATGTTTTTATTCAAAACGTAAAAAATAGAGCCTGCCGGCAGGACCGCGAAAAAATCCCGCCCCCCCCACTAGCCCGCCTGCCTGAGGCGGGCGCAC‑GCGTTGGACCAACCCCGCCAGG‑TGAAAAACGACAATAACGCCCGCCACAACGGCTAACAGATGAAACg                                                                                                                 <  1:559179/250‑1 (MQ=255)
                                                                                                                                          tAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTAGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGTGAAAA‑CGACGATAACGCCCGCCACAACGGCTAACAGCTGAAACGGCAAGCGACCGCCGTTaac                                                                                              >  2:253415/1‑250 (MQ=255)
                                                                                                                                                cAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGTGAAAC‑CGCCGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAAccctac                                                                                        >  2:164798/1‑247 (MQ=255)
                                                                                                                                                  aaGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGTGAAAA‑CGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGcc                                                                                      >  1:328392/1‑251 (MQ=255)
                                                                                                                                                          agcGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCCCTAGCTCGGCTGCCTGATGCTGGCGAAC‑GCGATTGAGCAATCCCGCCAGGGTGAAAA‑CGACGCTAACGCCCGCCACATCGGCTAACAGATGAAACGGCAAGCGACCGCCGTTACACCAAAGCCAGCGGCCg                                                                              >  2:291719/1‑251 (MQ=255)
                                                                                                                                                             ggcgATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGTGAAAA‑CGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGAtt                                                                           >  1:333841/1‑251 (MQ=255)
                                                                                                                                                               cgATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGATGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGTGAAAC‑CGCCGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGAAGTTAAACCATAGCCCGCGGCCGATTTc                                                                         >  1:23139/1‑251 (MQ=255)
                                                                                                                                                                 aTAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGTGAAAA‑CGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCg                                                                        >  1:286966/1‑250 (MQ=255)
                                                                                                                                                                      cAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGTGAAAA‑CGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCgaccg                                                                    >  1:679412/1‑249 (MQ=255)
                                                                                                                                                                                  cgcCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGCTTGAGCAATCCCGCCAGGGTGAAAA‑CGACGATAACGCCCGCcac                                                                                                                                     >  1:368772/1‑172 (MQ=255)
                                                                                                                                                                                     cAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGTGAAAA‑CGACGATAACGCCCGCCACAAAGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCGACCGAGACCGACATCACCATc                                                   >  2:487167/1‑251 (MQ=255)
                                                                                                                                                                                                  cgTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGTGAAAA‑CGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCGACCGAGACCGACATCACCATCAGGATCACCatat                                      >  1:160163/1‑251 (MQ=255)
                                                                                                                                                                                                             taTGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGGGAAAA‑CGACGATAACGCCCGCCACAACGGATAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGAGGCCGATTTCGACCGAGACCGACATCACCATCCGGATCCCCATAtttcaccatgcg                           >  2:387640/2‑242 (MQ=255)
                                                                                                                                                                                                                  gTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGTGAAAA‑CGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCGACCGAGACCGACATCACCATCAGGATCACCATATTTAGCGATGCGGAGAc                      >  1:235297/1‑251 (MQ=255)
                                                                                                                                                                                                                                      aTAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGTGAAAA‑CGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCGACCGAGACCGACATCACCATCAGGATCACCATATTTAGCGATGCGGAGACGGTCCCTTTCGGTAACTTAt  >  1:438990/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             
ATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAA‑TTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCAC‑GCGATTGAGCAATCCCGCCAGGGTGAAAA‑CGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCGACCGAGACCGACATCACCATCAGGATCACCATATTTAGCGATGCGGAGACGGTCCCTTTCGGTAACTTAT  >  NC_000913/4567028‑4567507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑