Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,560,309	T→A	P124P (CCA→CCT)	yjiH ←	uncharacterized protein YjiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,560,309	0	T	A	97.5%	112.5 / NA	40	P124P (CCA→CCT)	yjiH	uncharacterized protein YjiH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (16/23);  minor base C (1/0);  total (17/23)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAAC  >  NC_000913/4560072‑4560548                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                aaaTTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCcaaca                                                                                                                                                                                                                                    <  1:106842/251‑1 (MQ=255)         acacCAGGATGACGGCTAAAGGTACGATTACGGACGTGACCAGGAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGGTGGTGATTATGGCGTTACCGCGGGTTTGATATGCGGCGAAAATCACTTGATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTGTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGTCTGCGAGCAGATCCCAGGAATGCCAAACAACGGtttt                                                                                                                                                                                                                            <  2:602888/251‑1 (MQ=255)             cAGGATGAAGGCTAAAGGTACGATTACGGACGTGCCCAGACAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGATTGCTATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGGTAGCGATCAACGCCCGCCAGCCGATCCCAGGAATGCCCAACAACGGttatggc                                                                                                                                                                                                                         >  2:654859/1‑245 (MQ=255)                 aTGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTAAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAgg                                                                                                                                                                                                                    >  1:425660/1‑251 (MQ=255)                    aCGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGa                                                                                                                                                                                                                                              >  1:626134/1‑222 (MQ=255)                    aCGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGa                                                                                                                                                                                                                                              <  2:626134/222‑1 (MQ=255)                    aCGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATAACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGgtgt                                                                                                                                                                                                                 >  1:238932/1‑251 (MQ=255)                          aaaGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTa                                                                                                                                                                                                            <  1:385622/250‑1 (MQ=255)                                       aggACGTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTGGGTTATGATGGCGCTACCTCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTGCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTgcgcggcg                                                                                                                                                                                              <  2:178332/250‑1 (MQ=255)                                                 cAGAAAGGCAAACCCCCCAACGCCGGAAGAGAAGTAGGTGGTGATGATGGCTCGATCGTTGGGTTGATATGCGGCTAAAATCCCTTTATCGCTGTCGGGTATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGGTTTGCAGGTTAGCGTTCAGCGCCAGCTAGCAGATACCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCTCGGCGCGTAATccac                                                                                                                                                                                    <  2:668722/251‑1 (MQ=255)                                                        gCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCCGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGAcc                                                                                                                                                                             >  1:633858/1‑251 (MQ=255)                                                            acacCGGGACTCCGGAAGTGAAGTAGTTGGTGAGGATGGCTCTACCGCTTGTTTGATATGCGGCGAAAATCACTTTATCGCGGTCGGTAATTTCACCTTCCTGCGCCAGCGCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGGTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCg                                                                                                                                                                         <  2:588286/251‑1 (MQ=255)                                                              acCGCGACGCCGGAAGAGAAGGAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGCTAGCGATCAGCGCCAGCGCGCAGATCCCAGGAATACCCAACAACGGTTTTAGCACAGGTGTCATTAAATACTGCGCTGCGCGTAATCCACCAAGACCATctgt                                                                                                                                                                       >  2:627361/1‑248 (MQ=255)                                                                cgcgACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTaa                                                                                                                                                                     <  1:197065/251‑1 (MQ=255)                                                                        cGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGATAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCGCTTTAGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCTCGGCGCGTAAGCCACCAAGACCATCGGTAATTGAaata                                                                                                                                                             <  1:534691/251‑1 (MQ=255)                                                                         ggAAGAGAAGTAGGTGGTTATGATGTCGCTACCGCTGTGTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCATGAACATCGGTAATTGAaataa                                                                                                                                                            <  2:105585/251‑1 (MQ=255)                                                                                                       aCCGCTGGTTGGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCCCCGTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGAGCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCa                                                                                                                              <  2:431080/251‑1 (MQ=255)                                                                                                        ccGCTGGTTTTATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCGCCAAGACCAGCGGTAATTGATATAATCCCCAGAGAGAGAATGACTGACGGTGCCAg                                                                                                                             <  1:512979/251‑1 (MQ=255)                                                                                                                           cgAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAg                                                                                                          <  1:357587/251‑1 (MQ=255)                                                                                                                             aaaaTCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCAGCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGcc                                                                                                        <  1:370618/251‑1 (MQ=255)                                                                                                                                 tcacgttctCGCGTTCGGTAAATTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGAGGCGGCATCAGAGTTTTGCAGGTTAGCGCTCAGCGCCAGCGAGCAGGTCCCCGGAATGCCCAACAACGGTTTTAGCCCAGAAGGCATTAACTGCTGCCCATCGCGTCATCCACCAAGACCATCGGTAATTGCAATAATCCCCATGCAGAGAATGACATCCGGTGCCTGCTCCAACGCCAAGCGAAAGCCacct                                                                                                    >  2:76749/9‑248 (MQ=255)                                                                                                                                    cgttagtgccgtCGGTAACGTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTGAGGGATCAGCGCCAGCGAGCAGATTCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGAGCATCGGTAATTGAAATAATCCCCAGAGAGAGGATGACTGTCGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTa                                                                                                 <  1:45013/240‑1 (MQ=255)                                                                                                                                     tttATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGCGAATGACTGACGGTGCAAGCTCCAGCGCAAAGAGAAAGCCATCttt                                                                                                  >  1:404188/1‑249 (MQ=255)                                                                                                                                           gcgTTCGGTAATTCCACCTTCCTGCTCCAGCTTTTTGGTCATGCCGGCGGCGGCATCGGTGGGTTGCAGGTTAGCGAGCAGCGCCAGCGAGCTGATCCCAGGAATGCCCAACAACGGTCTTAGCTCAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGATAGAGAATGTCTGACGGTGCCAGCTCCAGCGCCAAGATAAGGCCATCTTTAGCCCCcg                                                                                          <  2:652578/251‑1 (MQ=255)                                                                                                                                            cgTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCcgc                                                                                         >  1:550476/1‑251 (MQ=255)                                                                                                                                                                       aGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAggtggg                                                              >  1:80265/1‑250 (MQ=255)                                                                                                                                                                                     aCCGGCGGCGGCATCGTTGGTTTGCAGTTTTGCGCTCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAAGAACGGTTTTAGCTCAGGTGGCATTAACTGCTGCTCGGGGCGTAATCCACCAAGACCAACGGTAATTGAAATAATCCCCAGAGAGAGAATGATTTACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTCGCCCCCGCGCCTCCCGCGCCGCGAAATGAGgtggtgtcgccgtgcgctc                                                <  2:48920/251‑14 (MQ=255)                                                                                                                                                                                      ccGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCAcc                                               <  1:652872/251‑1 (MQ=255)                                                                                                                                                                                           ggcggcATCGGAGTTTTGCAGGGTAGCGATCAGCGCGAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAGTCCACCACGACCATCGGAAATTGAAATAATCCCCAGCGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATATTTAGCCCCCGCGCCACCCGCGCCGCGAAATGCGGGGGGGGCTCCGTGCGCACAAGGcc                                          >  2:541260/1‑250 (MQ=255)                                                                                                                                                                                                     gtgtTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAg                                                                                                <  2:380453/187‑1 (MQ=255)                                                                                                                                                                                                     gtgtTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAg                                                                                                >  1:380453/1‑187 (MQ=255)                                                                                                                                                                                                        ttttGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACg                              >  1:211704/1‑250 (MQ=255)                                                                                                                                                                                                          ttGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCACAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGAt                            <  1:27796/250‑1 (MQ=255)                                                                                                                                                                                                           tGCAGGTGAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGTTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCAGCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATc                           <  2:633858/250‑1 (MQ=255)                                                                                                                                                                                                            gCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATcc                          <  1:290973/250‑1 (MQ=255)                                                                                                                                                                                                                   aGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGa                   >  1:414701/1‑250 (MQ=255)                                                                                                                                                                                                                      gATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCTAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACgg               >  1:385450/1‑251 (MQ=255)                                                                                                                                                                                                                           gcgcCAGCCAGCAGATCCCAGGAATGCCTAACAACGTTTTTAGCATAGGTGTCATTAACTGCTGCGCGACGCGTAATCCTCCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAATGAGAAAGCCATCTTTAGCCCCCGCGCCTCCCGCGTCGCGAGATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACg                <  2:192936/245‑1 (MQ=255)                                                                                                                                                                                                                               cAGCGAGCCGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCCCCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAACGACTAACGGAGCCAGCTCCCACGCAAAGAGAAAGCCATCTATAGTCCCCGCGACACCCGCGCCGCGAAATGCGCTGGTGGCTCCGCCCGACCCAGACAGTTGCCCACCCGATCCGCTCAGTACGAaaaactaa       >  2:393110/1‑246 (MQ=255)                                                                                                                                                                                                                                   gAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCACACGATCCGTTCAGAACGGAAAAATCAAAAAc  >  2:558594/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                AAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAAC  >  NC_000913/4560072‑4560548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑