Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,543,047 T→G intergenic (+414/‑68) fimE → / → fimA regulator for fimA/type 1 fimbriae major subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,543,0470TG96.0% 74.3 / ‑6.2 25intergenic (+414/‑68)fimE/fimAregulator for fimA/type 1 fimbriae major subunit
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (16/8);  total (17/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGAC  >  NC_000913/4542801‑4543289
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  
aattTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGCGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAAATCGTTTaca                                                                                                                                                                                                                                                 >  2:282221/1‑250 (MQ=255)
    tCACATCACCTCCCCTATATGTAAAGCTAACGTTTCTGTGGCTCGCCGCATCTTCCTCATTATTATCTCCCAAAACCACCTCCAGCAATCTAAACCTCTCTAACTAAAGATCACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATACTTTCCTCTATGAGTCAAAATGGCCCCAAATATTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTAGTTGaccaaac                                                                                                                                                                                                                                             >  2:285253/1‑250 (MQ=255)
                      atGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATAGTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATTCTTTCCTCTATGAGTCAAAATTGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTTTTGGCAGTCAAACTCTTTGACAAAACAAAGTGTACAGAACGACt                                                                                                                                                                                                                           <  2:420989/250‑1 (MQ=255)
                                         gtgGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCATCTATGACACAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGAAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGAATGCCCGTGTCGCTTTAgcagt                                                                                                                                                                                                       >  2:171745/1‑247 (MQ=255)
                                                       cTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGCTTTAGAAATAGTTTTTTgaaagg                                                                                                                                                                                         >  1:512217/1‑251 (MQ=255)
                                                                       tcCAAAAACCACCTCATGCAATATCAACATCTATAAATAAAGATCACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTAGTTGACACAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAAt                                                                                                                                                                         >  2:253405/1‑251 (MQ=255)
                                                                        ccAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAAtt                                                                                                                                                                        <  1:351175/251‑1 (MQ=255)
                                                                            aaaCCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTaaa                                                                                                                                                                     >  1:303107/1‑250 (MQ=255)
                                                                                  ccTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTgg                                                                                                                                                              >  2:559272/1‑251 (MQ=255)
                                                                                                  cATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTc                                                                                                                                              <  1:229075/251‑1 (MQ=255)
                                                                                                   aTCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTAAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCg                                                                                                                                             >  1:624095/1‑251 (MQ=255)
                                                                                                    tCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTTGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCgg                                                                                                                                            >  1:42701/1‑251 (MQ=255)
                                                                                                               aGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGAGAGGCTTTCCTCTATGAGACAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACACTGGCAATCGTTGTTCTGTCGGCTCTGTCCCt                                                                                                                                  >  2:209942/1‑250 (MQ=255)
                                                                                                                      aaTAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTACTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACAACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCt                                                                                                                           >  1:302708/1‑250 (MQ=255)
                                                                                                                             tatTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCgg                                                                                                                                            >  1:580074/1‑226 (MQ=255)
                                                                                                                             tatTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCgg                                                                                                                                            <  2:580074/226‑1 (MQ=255)
                                                                                                                                 aaGCCAACAAATACACTGAAAAAGTTTGTCCGCGTTGCTTTCCTCTATGAGTAAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAATCACAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGACAGCAGCATGCAAATTAATACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTAAGTTCTACAGCGGctc                                                                                                                 >  2:461917/1‑249 (MQ=255)
                                                                                                                                                        gTTTGTACGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCATTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTAAtgg                                                                                        <  2:245098/251‑1 (MQ=255)
                                                                                                                                                                   aTGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGGTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGGCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTAATGGTGGGACCGTTc                                                                             <  1:569078/251‑1 (MQ=255)
                                                                                                                                                                                                       ttCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAgttg                                                           <  1:590881/233‑1 (MQ=255)
                                                                                                                                                                                                       ttCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGATTTTTGAAAGGAAAGCAGAATGAAAATTAAAACTCTGGCAATCGTTGTTATGTCGGCTATGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGGCGGTTAATGGTGGGACCGTTCCCTTTAAAGGGGAAggtgaggtcggaaaggcgtggg                                         >  2:590881/1‑230 (MQ=255)
                                                                                                                                                                                                                             tgcagttttttcAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGGTGATc                    <  2:42701/239‑1 (MQ=255)
                                                                                                                                                                                                                               cAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGGACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAACGCAGAATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCACTCAGTTATACAGCGGCTCTGGCCGCTGCCACGACCGTTAATGGTGGGACCGTTAACTTTAAAGGGGTAGTTGTTAACGCTGCTTGCGCCGTTTATGTAGGCTCTGTTGATCaa                  >  2:568543/1‑250 (MQ=255)
                                                                                                                                                                                                                                   gtTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGt             >  1:38627/1‑251 (MQ=255)
                                                                                                                                                                                                                                                cTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGAc  >  1:91487/1‑249 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  
AATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGAC  >  NC_000913/4542801‑4543289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑