Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,540,431	T→C	intergenic (‑930/‑526)	nanC ← / → fimB	N‑acetylneuraminate outer membrane channel/Type 1 fimbriae regulatory protein FimB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,540,431	0	T	C	100.0%	90.2 / NA	27	intergenic (‑930/‑526)	nanC/fimB	N‑acetylneuraminate outer membrane channel/Type 1 fimbriae regulatory protein FimB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/13);  total (14/13)

TCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGGTGTTTTCAGGATGATGTTTCACTTAGTTTGTTTGCCGTATCGCCCGGCGAATGGCTGTGATTGAGGAAGGTTTAAGTCGTAGTGACCAAAGCTATATTTACCAACGAATGTAGATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCTAGGATAAAAAGGAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATGATAATAGCCACTAAGAAATTACTGCGCTCCATGAAATAGCCATTTTGTGGCAAATGGAGTTGACTAATAATGTCATATGTGAGACGGCTAGTTGAACGAATATTAAATTTTGCTGAATTTTTTATGTTGATTTTACTTGTTACAGAACATATCACATGATA  >  NC_000913/4540202‑4540675                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     tcatcaTGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGAGGTTGGTGTTATCAGGATGATGTTTCACTTAGTTTGTTTGCCGTATCGCCCGGCGAATGGCTGTGATTGAGGAAGGTTTAAGTCGTAGTGACCAAAGCTATATTTACCAACGAATGTAGATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCCAGGATAAAAAGGAATGTAAc                                                                                                                                                                                                                                  <  2:140823/250‑1 (MQ=255)  catcatTAAATATGTTTCCTGGTTTGTGGCTTGTTACTGGTCACTTCTGTAGTCGATCTGGAGAGGCTTGTTGATGTTGGTGGTTTCAGGATGATGTTTCACTTAGTTTGATTGCCGTATCGCCCGGCGAATGGCTGTGATTGAGGAAGGTTTAAGTCGTAGTGACCAAAGCTATATTTACCAACGAATGTAGATGAAAAAATCATCACCTGCGTTCCCCCATATCTCCAGGATAATAAGGAATGTAAc                                                                                                                                                                                                                                  <  2:576788/249‑1 (MQ=255)             atGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTTGTGTTTTCAGGATGATGGTTCACTTAGTTTGTTTGCCGTATCGCCCGGCGAATGGCTGTGATTGAGGAAGGTTTAAAGCGGAGTGACCAAAGCTATATTTACCAACGAATGGAGATGAAAAAATCATCTCCTGCGTTCCCCCCTATCTTCAGGATAAAAAGGAATGGAACAATCTCATGGCGt                                                                                                                                                                                                                     >  1:408983/1‑251 (MQ=255)                               cTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGGTGTTTTCCGGATGATGTTTCACTTAGTTTGTTTGCAGTATCGCCCGGCGAATGGCTGTGTTTGAGGAAGGTTTAAGTCGTAGTGACCAAAGCTATATTTACCAAAGAATGTAGATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCCAGGATAAAAAGGAATGTCACAATCTCATGGCGTAAGCTGCCGAATcagc                                                                                                                                                                                                     >  1:631540/1‑249 (MQ=255)                                                             ggAGAGGCTTGTTGATGTTGGTGTTTTCAGGATGATGTTTCACTTAGTTTGTTTGCCGTATCGCCCGGCGAATGGCTGTGATTGAGGAAGGTGTAAGTCGTAGTGACCAAAGCTATATTTACCAACGAATGTAGATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCCAGGATAAAAAGGAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAAt                                                                                                                                                                          <  2:315130/246‑1 (MQ=255)                                                               agagGCTTGTTGATGTTGGTGTTTTCAGGATGATGTTTCACTTCGTTTGTTTGCCGTATCGCCCGGCGAATGGCTGTGATTGAGGAAGGTTTAAGTCGTAGTGACCAAAGCTATATTTACCAACGAATGTAGATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCCAGGATAAAAAGGAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATg                                                                                                                                                                   >  1:237757/1‑251 (MQ=255)                                                                     ttgttgATGTTGGTGTTTTCAGGATGATGTTTCACTTAGTTTGTTTGCCGTATCGCCCGGCGAATGGCTGTGATTGAGGAAGGTTTAAGTCGTAGTGACCAAAGCTATATTTACCAACGAATGTAGATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCCAGGATAAAAAGGAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATGaaaat                                                                                                                                                              >  2:576173/1‑250 (MQ=255)                                                                                       tCAGGATGATGTTTCACTTAGTTTGTTTGCCGTATCGCCCGGCGAATGGCTGTGATTGAGGAAGGTTTAAGTCGTAGTGACCAAAGCTATATTTACCAACGAATGTAGATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCCAGGATaaa                                                                                                                                                                                                                                              >  1:444236/1‑152 (MQ=255)                                                                                                    tcccttatgttgttgtccGTATAGCCCGGCGAATGGCTTTGATTTATGAAGGTTTAAGTCGTAGTGACCAAAGCTATATTTACCAACGAATGTAGATGAAAAAATCATCGCCTGCGTTCCCCCATATCTCCAGGATAAAAAGTAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATGATAATAGCCACTAAGAAATTACTGCGCTCCATGaaa                                                                                                                               <  2:640229/234‑1 (MQ=255)                                                                                                         tAGTGTGTTTGCCGTATCGCCCGGCGAATGGCTGTGATTGAGGAAGGTTTAAGTCGTAGTGACCAAAGCTATATTTACCAACGAATGTAGATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCCAGGATAAAAAGGAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATGATAATAGCCACTAAGAAATTACTGCGCTCCATGAAATAGcc                                                                                                                          <  2:170373/250‑1 (MQ=255)                                                                                                            tttgtttgCCGTATCGCCCGGCGAATGGCTGTGATTGAGGAAGGTTTAAGTCGTAGTGACCAAAGCTATATTTACCAACGAATGTAGATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCCAGGATAAAAAGGAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATGATAATAGCCACTAAGAAATTACTGCGCTCCATGAAATAGCCAttt                                                                                                                      >  1:130299/1‑251 (MQ=255)                                                                                                                        aTCGCCCGGCGAATGGCTGTGATTGAGGAAGGTTTAAGTCGTAGTGACCAAAGCTATATTTACCAACGAATGTAGATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCCAGGATAAAAAGGAATGTAACAATCTCATgg                                                                                                                                                                                                                        <  1:388627/141‑1 (MQ=255)                                                                                                                        aTCGCCCGGCGAATGGCTGTGATTGAGGAAGGTTTAAGTCGTAGTGACCAAAGCTATATTTACCAACGAATGTAGATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCCAGGATAAAAAGGAATGTAACAATCTCATgg                                                                                                                                                                                                                        >  2:388627/1‑141 (MQ=255)                                                                                                                        aTCGCCCGGCGAATGGCTGTGATTGAGGAAGGTTTAAGTCGTAGTGACCAAAGCTATATTTACCAACGAATGTAGATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCCAGGATAAAA‑GGAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATGATAATAGCCACTAAGAAATTACTGCGCTCCATGAAATAGCCATTTTGTGGCAAATGGa                                                                                                         <  1:658018/251‑1 (MQ=255)                                                                                                                             ccGGCGAATGGCTGTGATTGAGGAAGGTTTAAGTCGTAGTGACCAAAGCTATATTTACCAACGAATGTAGATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCCAGGATAAAAAGGAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATGATAATAg                                                                                                                                                            >  2:236090/1‑196 (MQ=255)                                                                                                                             ccGGCGAATGGCTGTGATTGAGGAAGGTTTAAGTCGTAGTGACCAAAGCTATATTTACCAACGAATGTAGATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCCAGGATAAAAAGGAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATGATAATAg                                                                                                                                                            <  1:236090/196‑1 (MQ=255)                                                                                                                               ggCGAATGGCTGTGATTGAGGAAGGTTTAAGTCGTAGTGACCAAAGCTATATTTACCAACGAATGTAGATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCCAGGATAAAAAGGAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATGATAATAGCCACTAAGAAATTACTg                                                                                                                                           <  2:517794/211‑1 (MQ=255)                                                                                                                               ggCGAATGGCTGTGATTGAGGAAGGTTTAAGTCGTAGTGACAAAAGCTATATTTACCAACGAATGTAGATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCCAGGATAAAAAGGAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATGATAATAGCCACTAAGAAATTACTg                                                                                                                                           >  1:517794/1‑211 (MQ=255)                                                                                                                                                                       ccAAAGCTATATTTACCAACGAATGTAGATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCCAGGATAAAAAGGAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATGATAATAGCCACTAAGAAATTACTGCGCTCCATGAAATAGCCATTTTGTGGCAAATGGAGTTGACTAATAATGTCATATGTGAGACGGCTAGTTGAACGAATAtt                                                           >  1:307697/1‑251 (MQ=255)                                                                                                                                                                          aaGCTATATTTACCAACGAATGTAGATGAAAAAATCATCTCCTGCGGTCCCCCATATCTCCAGGATAAAAAGGAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATGATAATAGCCACTAAGAAATTACTGCGCTCCATGAAATAGCCATTTTGTGGCAAATGGAGTTGACTAATAATGTCATATGTGAGACGGCTAGTTGAACGAATATTaaa                                                        <  2:270125/251‑1 (MQ=255)                                                                                                                                                                                           gAATGTAGATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCCAGGATAAAAAGGAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATGATAATAGCAACTAAGAAATTACTGCGCTCCATGAAATAGCCATTTTGTGGCAAATGGAGTTGACTAATAATGTCATATGTGAGACGGTTAGTTGAACGAATATTCAATTTTGCTGAAttttttt                                       >  2:338502/1‑250 (MQ=255)                                                                                                                                                                                               gttgATGAAAAAATCATCCCCTTCGTTCCCCCAGATCGCCAGGATAAAAAGGAATGTAACAATCCCATGGCATAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCCTTATAATAGCCACTAAGAAATTACTGCGCTCCATGAAATAGCCATTTTGTGGCAAATGGAGTTGACTAATAATGTCATTTGTGAGAAGGCTATGTGAACGAATATTAAATTCTGCTGAAGTTTTTATGta                                   <  2:188424/248‑2 (MQ=255)                                                                                                                                                                                                  gATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCCAGGATAAAAAGGAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATGATAATAGCCACTAAGAAATTACTGCGCTCCATGAAATAGCCATTTTGTGGCAAATGGAGTTGACTAATAATGTCATATGTGAGACGGCTAGTTGAACGAATATTAAATTTTGCTGAATTTTTTATGTTGAt                                <  1:576173/251‑1 (MQ=255)                                                                                                                                                                                                                     gCGTTCCCCCATATCTCCAGGATAAAAAGGAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATGATAATAGCCACTAAGAAATTACTGCGCTCCATGAAATAGCCATTTTGTGGCAAATGGAGTTGACTAATAATGTCATATGTGAGACGGCTAGTTGAACGAATATTAAATTTTGCTGAATTTTTTATGTTGATTTTACTTGTTACAGAACat             >  1:10722/1‑251 (MQ=255)                                                                                                                                                                                                                       gTTCCCCCATATCTCCAGGATAAAAAGGAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATGATAATAGCCACTAAGAAATTACTGCGCTCCATGAAATAGCCATTTTGTGGCAAATGGAGTTGACTAATAATGTCATATGTGAGACGGCTAGTTGAACGAATATTAAATTTTGCTGAATTTTTTATGTTGATTTTACTTGTTACAGAACatat           >  1:323628/1‑251 (MQ=255)                                                                                                                                                                                                                         tCCCCCATATCTCCAGGATAAAAAGGAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATGATAATAGCCACTAAGAAATTACTGCGCTCCATGAAATAGCCATTTTGTGGCAAATGGAGTTGACTAATAATGTCATATGTGAGACGGCTAGTTGAACGAATATTAAATTTTGCTGAATTTTTTATGTTGATTTTACTTGTTACAGAACATATc          >  1:603414/1‑250 (MQ=255)                                                                                                                                                                                                                                aatCTCCAGGATAAAAAGGAATGTAACAAACTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATGATAATAGCCACTAAGAAATTACTGCGCTCCATGAAATAGCCATTTTGTGGCAAATGGAGTTTACTAATAATGTCATATGTGAGACGGCTAGTTGAACGAATATTAAATTTTGCTGAATTGTTTATGTTGATTTTACTTGTTACAGAACATATCACATGata  <  2:130299/250‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     TCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGGTGTTTTCAGGATGATGTTTCACTTAGTTTGTTTGCCGTATCGCCCGGCGAATGGCTGTGATTGAGGAAGGTTTAAGTCGTAGTGACCAAAGCTATATTTACCAACGAATGTAGATGAAAAAATCATCTCCTGCGTTCCCCCATATCTCTAGGATAAAAAGGAATGTAACAATCTCATGGCGTAAGCTGACGAATCAGCAGGAATAATCGCTAGGGACCTAAGAATTAGCATGATAATAGCCACTAAGAAATTACTGCGCTCCATGAAATAGCCATTTTGTGGCAAATGGAGTTGACTAATAATGTCATATGTGAGACGGCTAGTTGAACGAATATTAAATTTTGCTGAATTTTTTATGTTGATTTTACTTGTTACAGAACATATCACATGATA  >  NC_000913/4540202‑4540675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑