Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	939	G→A	V201V (GTG→GTA)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	939	0	G	A	100.0%	131.2 / NA	45	V201V (GTG→GTA)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/14);  total (31/14)

CGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTA  >  NC_000913/698‑1183                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     cgctgATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGATATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCCTTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGGGATGGTGGCAGGTTTCACCCCCGGTAATGAAAAAGGCGAACTGGGGGAACTTGGACGc                                                                                                                                                                                                                                             >  2:128778/1‑251 (MQ=255)   ctgATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGGGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCa                                                                                                                                                                                                                                            >  1:411671/1‑250 (MQ=255)     gATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGGACTTGGACGCaccg                                                                                                                                                                                                                                         >  1:524860/1‑248 (MQ=255)                 cGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCCACGGTTCCGacacct                                                                                                                                                                                                                             >  2:669351/1‑247 (MQ=255)                        aTGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACAGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGCCGCAACGGTTCCGACTActccgcggc                                                                                                                                                                                                                      >  1:43059/1‑245 (MQ=255)                                      tATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGGGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGGAATGAAAAAGGCGAACTGGCGGGACTTGGGCGCAACGGTTCCGACTActccgaggcggggggtggtgacc                                                                                                                                                                                                        >  2:581259/1‑231 (MQ=255)                                          gCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGAGGTGGCTGCCTGttt                                                                                                                                                                                                    >  2:441901/1‑251 (MQ=255)                                             ggCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTa                                                                                                                                                                                                   >  1:428024/1‑249 (MQ=255)                                                  aTTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGGCGCCTGTTTTCGCGccc                                                                                                                                                                                            >  2:94603/1‑250 (MQ=255)                                                        aGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCGTTTTACGCCCCGgttgtt                                                                                                                                                                                      >  2:88358/1‑251 (MQ=255)                                                                              gTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGCTATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTTCGAGATTTGGGAGGACGTTgg                                                                                                                                                                >  1:583021/1‑250 (MQ=255)                                                                                 aTCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGCTTGTTGAGAGATTTGGACGGACGTTGACgg                                                                                                                                                             >  1:55842/1‑251 (MQ=255)                                                                                   cGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGAGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAACAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGGTGGCTGCCTGTTTACGCGCCGATTGTTGCGGGATTTGGAGCGACGTTGACgggg                                                                                                                                                           >  1:669483/1‑251 (MQ=255)                                                                                                     gctgGCAGTGGGGCATTACCTCGAATATACCGTCGATATTGCTGAGTCCACCCGCAGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGGATTGGACGGACGGTGACGGGGGCGATACCTGCGACCcgc                                                                                                                                         >  1:250280/1‑251 (MQ=255)                                                                                                       tgGCAGTGGGGAATTACCTCGAATCTACCGTCGATATTGCTGAGACCACCCGCCGTAATGAGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACAACTCAGCGACGGGGGCGGCGGCCTGTTTACGAGCCGATTGGTGAGAGGATTGGACGGGCGTTGAGgggggggatacaggcgagccgcgg                                                                                                                                       >  2:642952/1‑231 (MQ=255)                                                                                                        ggCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGACGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGAAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGGGCTGGCTGCCTGTTTACGGGACGATTTTTGCGAGATTTGGGCGGACGTTGACGGGGTCTTTACCTGCGACCCGCGTc                                                                                                                                      >  1:2111/1‑251 (MQ=255)                                                                                                                               cTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCCGGTTTCACCGCCGGTAATGAAAAAGGCGACCTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCCGTTTCCGCGCCGGTTGTTGCGAGTGTTGGGCGGAAGTTTAAGGGGGCTATGCCTGCGACCCGCGTCAGGTTACCGATGCGAGGgtgttg                                                                                                               >  2:368464/1‑251 (MQ=255)                                                                                                                               cTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGGTTTGGACGGACGTTGACGGGGTCTAAACCTGCGACCCGCGGCAGGGGCCCGATGCGAGGttgttg                                                                                                               >  1:450362/1‑251 (MQ=255)                                                                                                                                ccggtgtttcttggttgcccccccccGCCTTATGGCGGCAAGTGGCATTCCGGTTTATCACAGGGTGCTGAGGGCGGGTTTCCCCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGa                                                                                                              <  1:581259/228‑1 (MQ=255)                                                                                                                                  cggttaattggcatctcccccccggccgTTTTGGGGAAAGGCGCATTCCGGTTGATCCAATGGGGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAg                                                                                                            <  1:142722/227‑1 (MQ=255)                                                                                                                                                gAGTCCACCCGCCGTATTGAGGCAAGTCGCATTCCGGATGATCACCTGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGGGCTGGCTGCCTGTTTACGGCCCGGATGTTGCGCGGTTTGGACGGACGTTGGCGGGGTTTATACCTTCAACCCCCGTCAGGGGCCCGGTGGGAGGGTGTTGAAggcggtggctccacc                                                                                              >  2:290775/1‑237 (MQ=255)                                                                                                                                                     cACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGGGAGGTTTGGACGGAGGTTGCCGGGGTCTATACCTGCGCCCCGCGGCAGGTGCCCGCAGCGAGGGTGTTGAAGTCGATGTCCtccccgga                                                                                           >  2:292341/1‑242 (MQ=255)                                                                                                                                                           ccgTTTTGCGGCAAGTCGCATTCCGGCGGATCACATGTGGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATgg                                                                                   <  1:590559/251‑1 (MQ=255)                                                                                                                                                                     gcagtgcgcttcccggcgaacaacaTGGTGCTGGTGGCAGGTTCCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGGCGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTa                                                                         <  1:290775/229‑1 (MQ=255)                                                                                                                                                                     gCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTAACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGGGGTTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGGGCCCGGTGCGGGGTTGTTGAAGTCGATGTCCTACCAggcagtagagtgggagggcgt                                                                         >  2:596651/1‑232 (MQ=255)                                                                                                                                                                     gCAAGTCGCATCCGGGCTGATCACAGGGTGCGGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGc                                                                                        <  1:596651/236‑1 (MQ=255)                                                                                                                                                                       aagtcGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCCGGTGCCCGATGCGGGGTTGTTTGAGTCGCTGTCCTACCAGGAAGCGATGGCGCTTTactact                                                                       >  1:679959/5‑251 (MQ=255)                                                                                                                                                                        agtcGCATTCCGGCTGATCACATGGTGCTGATGGAAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGGTTACCCGCCGAATGTTGCGGGAGTTGGACGGACGTTGACGGGGTCTATTCCTGCGACCCGCGTCAGGTGCCCGGTGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGAGATGGAGCTTTCCTCCtt                                                                      >  1:184035/4‑251 (MQ=255)                                                                                                                                                                              aTTCCGTCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTCATGAAAAAAGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCAGGGCTGGCTGCCTGTTTACGCGCCGCTTGTTGCGGGATTTTGACGGACGATTAAGGGGTCTATAACTGCGCCCCGAGTCAGGGGTCCGGTGGGAGGTTGGTGGAGTCCACATCCCACCAGGAAGCGATAGAGGTTTctccgtttggggc                                                                >  2:123992/1‑239 (MQ=255)                                                                                                                                                                                 ccGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGGTTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTa                                                              >  1:514732/1‑250 (MQ=255)                                                                                                                                                                                           acaTGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCAc                                                   >  1:129114/1‑251 (MQ=255)                                                                                                                                                                                            gatgggtctgttGGCATGTTTAACCGCGCGTAATGAACAAGGCGAACTGGTGGGACTTGGACGCAACGGTTCCGATTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTTGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCAcc                                                  <  2:452408/240‑1 (MQ=255)                                                                                                                                                                                              gtgtgcggtgggcggttttCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCAccc                                                 <  1:15485/234‑1 (MQ=255)                                                                                                                                                                                              gggTGCTGATGGCAGGTTACACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCAcccc                                                <  1:21047/250‑1 (MQ=255)                                                                                                                                                                                                  gCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCa                                            <  1:94603/251‑1 (MQ=255)                                                                                                                                                                                                   gtgagggcgggTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCAc                                           <  1:669351/242‑1 (MQ=255)                                                                                                                                                                                                                tacacgccgGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCTGCGGTGCTTGCTGCCTGTTTACGGGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCTCCGCACCATTACCCCCAt                               <  2:428024/246‑1 (MQ=255)                                                                                                                                                                                                                      ccgGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGGAGTCGATGTCCTACCAGGAAGCGGTGGGGCTTTCCTACTTCGGCGCTAAAATTCTTCACCCCCGCACCCTTACCCCCaaccccc                          >  1:594500/1‑243 (MQ=255)                                                                                                                                                                                                                        ggTAATGAAAAAGGCGAACTGGTGGTACTGGGTCGCAACGGGGCCGACTACTCCGCGGCGGGGCTGGCTGCCTGGTTACGCGCCGATGGTTGCGAGATGTGGACGGACGATGACGGGGTCTATACCTGCGACCCGCGTCAGGTGACCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACAATTACCCCCATCGCCAAGtt                      <  2:170748/251‑1 (MQ=255)                                                                                                                                                                                                                               aaaaaGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTcc                    >  1:239904/1‑246 (MQ=255)                                                                                                                                                                                                                                   atgCGAACTGGTGGTACTTGGACGCATCGGTTCCGACTACTCCGCGGCGGTGCTGGCTGACTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCtt           <  1:494519/249‑1 (MQ=255)                                                                                                                                                                                                                                     gCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGGTGTTGAAGTCGATGTCCTAACAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATACCttcc         >  1:403860/1‑249 (MQ=255)                                                                                                                                                                                                                                      cGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGcc        >  1:63041/1‑251 (MQ=255)                                                                                                                                                                                                                                       gAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCCCCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCt       >  1:284096/1‑251 (MQ=255)                                                                                                                                                                                                                                            ggtggtACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTa  <  1:75027/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     CGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTA  >  NC_000913/698‑1183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑