Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,532,063	C→T	intergenic (‑412/+374)	sgcX ← / ← yjhP	putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,532,063	0	C	T	100.0%	196.9 / NA	63	intergenic (‑412/+374)	sgcX/yjhP	putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/29);  total (34/29)

TGGAGAGACCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCACTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTG  >  NC_000913/4531819‑4532304                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  tGGAGAGACCAGTGATTATGGTTGAAATAATGAGGCTGACTAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCg                                                                                                                                                                                                                                             >  1:563618/1‑251 (MQ=255) tGGAGAGACCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCACGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAACCATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCg                                                                                                                                                                                                                                             >  2:5348/1‑251 (MQ=255)  ggAGAGACCAGTGATGATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACGTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTcc                                                                                                                                                                                                                                              <  1:368113/249‑1 (MQ=255)            gTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCTCCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGcc                                                                                                                                                                                                                                  <  1:221547/251‑1 (MQ=255)              gATTATTGTTTAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGctca                                                                                                                                                                                                                                <  2:563618/251‑4 (MQ=255)                ttATGGTTGAAATAATGAGGCTGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAgg                                                                                                                                                                                                                              >  2:604717/1‑251 (MQ=255)                        gAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTcc                                                                                                                                                                                                                                              <  1:457082/227‑1 (MQ=255)                         aaaTAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCCCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTg                                                                                                                                                                                                                     <  2:49470/251‑1 (MQ=255)                              atGAGGATGAATAAACCGCGGTAACTGTTATAGCACCACTTTGTTGTAGGCCGCTCCCTCTTCATTACGTCCTGAACGTGGATACCCCAGCATCTCCATCAGCGGCTCTTAGACTGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTCAGAAAACATCGGTTCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCAGCTTTATGAGCCCAGGCTGGCATTGCCgcc                                                                                                                                                                                                                >  2:291769/1‑249 (MQ=255)                              atGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTc                                                                                                                                                                                                                >  1:341461/1‑251 (MQ=255)                                gAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCCCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCCTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTTTGAGCCCAGGCTGGCATTGCCGTCa                                                                                                                                                                                                               <  1:402993/250‑1 (MQ=255)                                   gATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGAt                                                                                                                                                                                                            >  1:505943/1‑250 (MQ=255)                                      aaaTAAAACGCGGTAACTGTTATAGCCCCACTTTGTTGCAGGACGCTCCCTCTGCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTGCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGAGTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCTTCAGATagcg                                                                                                                                                                                                        <  2:218133/250‑4 (MQ=255)                                            aaCGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGc                                                                                                                                                                                                  <  1:115438/251‑1 (MQ=255)                                                  gTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCtt                                                                                                                                                                                            >  2:598236/1‑251 (MQ=255)                                                        gTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGc                                                                                                                                                                                      >  1:16094/1‑251 (MQ=255)                                                           ataGCACCACTTTGTTGCAGGACGCTCCATCTTCATTACGTCCTGAACGTGGATAACCCAGCATCTCCATCAGCGGCTCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGCTCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCCAATCATTCTCCGCTTTATGAGCCAAGGCTGGCATTGCCGTCAGATAACGATTTTCACGCtaatagacttt                                                                                                                                                                                   >  2:414135/1‑241 (MQ=255)                                                             aGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTACATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTtcc                                                                                                                                                                                  <  2:259061/250‑1 (MQ=255)                                                                    cTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGcc                                                                                                                                                                          >  1:371909/1‑251 (MQ=255)                                                                      ttgttgCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCg                                                                                                                                                                         <  1:598236/250‑1 (MQ=255)                                                                        gttgCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACt                                                                                                                                                                      >  1:214049/1‑251 (MQ=255)                                                                              ggACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGAGCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGc                                                                                                                                                                <  2:341461/251‑1 (MQ=255)                                                                               gACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGcc                                                                                                                                                               <  1:596812/251‑1 (MQ=255)                                                                                 cGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAg                                                                                                                                                             <  2:505943/251‑1 (MQ=255)                                                                                    tCCCTCTTCATTCCGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCGGGCTGGCATTGCCGGCAGATAACGTTTTGCCCGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGa                                                                                                                                                           >  2:671641/1‑250 (MQ=255)                                                                                    tCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGa                                                                                                                                                           >  1:646911/1‑250 (MQ=255)                                                                                            cATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGGTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGaa                                                                                                                                                  <  2:117697/251‑1 (MQ=255)                                                                                                     cTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAACATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGATCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTTTGTGCTTCCTCCGGGCCGACTTTTTGCACGGACTCAAGGAACCTGGCTa                                                                                                                                          >  2:573586/1‑250 (MQ=255)                                                                                                             ggATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGAt                                                                                                                                  >  2:560947/1‑250 (MQ=255)                                                                                                               aTACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCATCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATAcc                                                                                                                               >  1:332082/1‑251 (MQ=255)                                                                                                                tACCCCAGCATATCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACATGATACCg                                                                                                                              >  2:96703/1‑251 (MQ=255)                                                                                                                  ccccAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGct                                                                                                                            >  1:199287/1‑251 (MQ=255)                                                                                                                  ccccAGCATCGCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGct                                                                                                                            <  1:671641/251‑1 (MQ=255)                                                                                                                                tcagaggctCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAGGCTGGCTCAACCTGATACCGCTTTTTCCCAgggatt                                                                                                              >  2:293505/9‑248 (MQ=255)                                                                                                                                 cAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTg                                                                                                             >  1:161781/1‑251 (MQ=255)                                                                                                                                   gCGGATCTTAGCATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGAATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTTTGAGGCCAGGCTGGCATTGCCGACAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCATGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTgag                                                                                                           <  1:105669/251‑1 (MQ=255)                                                                                                                                     ggATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGCTAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAACCCTGATACCGCTTCTTCCCAGGGTTTg                                                                                                             >  1:444472/1‑247 (MQ=255)                                                                                                                                           tAGAAGGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCg                                                                                                    <  2:161781/250‑1 (MQ=255)                                                                                                                                               aTGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCt                                                                                               >  1:580602/1‑251 (MQ=255)                                                                                                                                                           gTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCAtt                                                                                    >  1:289717/1‑250 (MQ=255)                                                                                                                                                            gggCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTAc                                                                                  <  2:646911/250‑1 (MQ=255)                                                                                                                                                             ggCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACg                                                                                 <  2:214049/251‑1 (MQ=255)                                                                                                                                                               cAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGca                                                                               <  1:49995/251‑1 (MQ=255)                                                                                                                                                                        ggTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTg                                                                       >  2:210155/1‑250 (MQ=255)                                                                                                                                                                        ggTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTg                                                                       <  1:35138/250‑1 (MQ=255)                                                                                                                                                                                  aTAATGTAAGAAAACATCGGATATCCTGAATATATTCAGAGGGAGTATAGATAATCAGCTCAAAGCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAATCCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTTATCACTGGCAc                                                            <  2:371909/251‑1 (MQ=255)                                                                                                                                                                                      tGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTACGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGACGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGCGTTTGCCGTTGCTGTCGCTGCATTACGCCCAATATTGATCAATGGCACtgtt                                                        >  2:352094/1‑250 (MQ=255)                                                                                                                                                                                              aaCATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTg                                                >  1:258507/1‑251 (MQ=255)                                                                                                                                                                                                      aTCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCTCAATATTGATCACTGGCACTGTGTGCATCTGGGGCCGAc                                        >  2:264716/1‑251 (MQ=255)                                                                                                                                                                                                         tcCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATc                                     >  1:241650/1‑251 (MQ=255)                                                                                                                                                                                                          ccTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATTATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTAGGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCTCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACAt                                      <  2:673029/249‑1 (MQ=255)                                                                                                                                                                                                          ccTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGACGACATCg                                    >  2:80969/1‑251 (MQ=255)                                                                                                                                                                                                              aaTAGATTCAGAGAGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGTAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGAACTGTGTGCATCTGGTGCCGACATCGGCAt                                >  2:160927/1‑251 (MQ=255)                                                                                                                                                                                                                        gcgggAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGCTTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGcc                      >  2:409087/3‑251 (MQ=255)                                                                                                                                                                                                                           ggAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGa                   <  1:560947/251‑1 (MQ=255)                                                                                                                                                                                                                              gTATAGATAATCAGCTCAAATAATTCTCCGCTTTATGCGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCATCCGGGCCGACTTTTTGCCAGGACTCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCTTCTGTTGCCTGCATCGACCTTCCGCAAGACTGAc                  >  2:50898/1‑249 (MQ=255)                                                                                                                                                                                                                                  aGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCTAGGCTGGCAGTGCCGTCAGATAACGTTTGGCACGCTTAGGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACt            <  2:182220/251‑1 (MQ=255)                                                                                                                                                                                                                                   gATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTg           <  1:89500/251‑1 (MQ=255)                                                                                                                                                                                                                                     aaaTCAGCCCAAATCATTATCCGCTTTCTGAGCCCAGGCTGGCATTGACGTCCGATAACCCTTTGCACGCTTATGTGCTGCCTCCGGGCCGACTTTTTGCCAGGTATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTTCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATAATGATCACTGTCACTGTGTGCATCTGGTGCCGACATCGTCATTCCGCAAGCCTTACAGAACTGtt         <  2:544398/250‑1 (MQ=255)                                                                                                                                                                                                                                      aaTCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGACGACATCGGCATTCCGCAAGCCTGCCAGAACTGTTc        >  2:321776/1‑251 (MQ=255)                                                                                                                                                                                                                                       agcAGCTCAAATCATGCCCCGCTTTATGAGCCCAGGCTTGCATTGCCTTCAGATAACGTTTTGCACGCTGATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAAGCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACCGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGATAGAACTGTTCa       <  2:16094/249‑1 (MQ=255)                                                                                                                                                                                                                                           gCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCTGACATCGGCATGCCGCAAGCCTGACAGAACTGTTCAtgtt   <  1:210155/251‑1 (MQ=255)                                                                                                                                                                                                                                            cTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCAtgttg  >  1:159592/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  TGGAGAGACCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCACTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTG  >  NC_000913/4531819‑4532304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑