Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,510,238	T→G	intergenic (+445/+452)	insO → / ← fecE	IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,510,238	0	T	G	100.0%	141.9 / NA	44	intergenic (+445/+452)	insO/fecE	IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (23/21);  total (23/21)

GCGCTTTCCCTCTGGCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTG  >  NC_000913/4509997‑4510475                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              gcgcTTTCCCTCTGGCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCAAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAAAGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTACGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCCATTCAGTTCCAGGGTTGCttt                                                                                                                                                                                                                                      >  1:438044/1‑251 (MQ=255)          ctctGGCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGCTCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACg                                                                                                                                                                                                                             >  1:506307/1‑251 (MQ=255)              ggCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGtt                                                                                                                                                                                                                           <  1:347801/249‑1 (MQ=255)                 gacaaaGGGCGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGATTCGGTAAGCTGGGTGAGACCCCGACTCATGACGATCTCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGCACGCTGCACCTACCTAAGTAGTAGCCAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTAAGTTGGCGt                                                                                                                                                                                                                      >  2:95363/4‑251 (MQ=255)                      aGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTgtt                                                                                                                                                                                                                 >  2:41265/1‑249 (MQ=255)                             aGGACGGGCCCGTCTGCCTTCACTTTATGCAAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGCTCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAACGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAACCTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAt                                                                                                                                                                                                          >  2:90713/1‑251 (MQ=255)                                 cgtgcccgtaggCCTTCACTTTATGCCAGCTTCGGTAATCTGGGTGAGACACCGACTCATGAAGATATCAGTGATAACAATCGATCCATCATCTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTGTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTATGTTGGCGTCTGAGCATTGATTTAt                                                                                                                                                                                                      <  2:55340/240‑1 (MQ=255)                                            gCCTTCATTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATAGCAGTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGAGTCTGATCATTGATTTATCCTCAAAAGcc                                                                                                                                                                                           <  2:582709/251‑1 (MQ=255)                                                 cACTTTATGCCAGCTTCGTTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAAcc                                                                                                                                                                                      >  2:512415/1‑251 (MQ=255)                                                 cACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCGTTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGACTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCAACCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGTTTTACGTTGGGGTCTGATCATTGATTTATCCTCAAAAGCCCAAcc                                                                                                                                                                                      >  2:294752/1‑251 (MQ=255)                                                                     aaGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCACCTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGTTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTTGTAATGGACCAGCt                                                                                                                                                                  >  1:255078/1‑251 (MQ=255)                                                                      aGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTc                                                                                                                                                                 >  1:529786/1‑251 (MQ=255)                                                                       gCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTcc                                                                                                                                                                <  1:528693/251‑1 (MQ=255)                                                                          gggTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGt                                                                                                                                                              <  1:196469/250‑1 (MQ=255)                                                                                 acacCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCGAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTcc                                                                                                                                                      <  2:554475/251‑1 (MQ=255)                                                                                   acCGACCCATGACGATCTCTTTGCTAACAATCGATCCATCAACTGGCCTGTATAACGTAGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCACACGCACACTTTTTAGAAGAATCGATGGTCAGACACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCAACCCTCATTGGTAATGAACCAGCTCCGTGAATGTACGc                                                                                                                                                    >  2:57185/1‑251 (MQ=255)                                                                                                     cATTGATAACAATCGATCCAACAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAATTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAAt                                                                                                                                  <  1:404706/251‑1 (MQ=255)                                                                                                            aaCAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTg                                                                                                                            <  1:490457/250‑1 (MQ=255)                                                                                                            aaCAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGGGATTAAGAAAGCTGCACCTACCTAAGAAGTAGCAAACGCACACTTTTTAGAAAAATCAATGGTAAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGACGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACAAGCTCCGTGAGTGTCCGCTCTGGCACAAAGCGAAATTTTTTg                                                                                                                            >  1:248588/1‑250 (MQ=255)                                                                                                                    aTCCATCAACTGGACTGTATAACGTTGCTGCCGGTGATTAATAAAGCTTCTCCTACCTAAGTAGTAGCAAACGCACACTGTTTAGAAAAATCGATGGTCAGAAAATGGAGTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGAgctc                                                                                                                       <  2:588461/247‑1 (MQ=255)                                                                                                                           aaCTGGCCTGTATAACGGGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCCCAGAGCGAAATTTTTTGATCTCCCACCTGAAAt                                                                                                            >  2:543742/1‑251 (MQ=255)                                                                                                                            aCTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATc                                                                                                           >  1:677071/1‑251 (MQ=255)                                                                                                                               ggCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTa                                                                                                         >  1:88158/1‑250 (MQ=255)                                                                                                                                              gCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCAc                                                                                         <  1:28127/251‑1 (MQ=255)                                                                                                                                                            aGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCt                                                                           <  1:514502/251‑1 (MQ=255)                                                                                                                                                               aaGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTaa                                                                         >  2:200457/1‑250 (MQ=255)                                                                                                                                                                   tGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCCGAAACTGGATTAGCAATTCCGATCCAGGGTTGCTTTTGATTTACGATGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCATGAAATCTAAACTTCGTCATGTCACGTTTTTGGGTTTCTAAAAttt                                                                    >  2:126321/1‑251 (MQ=255)                                                                                                                                                                    gctcctacataAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGTTTTTGATTTACGTTGGCGTCTGATCATTGATTTTTCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCGGAAATCTAATCTTAGTCATGTCACGTTTTTGGTTTTCTAAAAtt                                                                     <  2:216053/246‑1 (MQ=255)                                                                                                                                                                            ctaAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTc                                                                                                                                                       >  1:530193/1‑159 (MQ=255)                                                                                                                                                                            ctaAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTc                                                                                                                                                       <  2:530193/159‑1 (MQ=255)                                                                                                                                                                                   gtagCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGAAATTCCGTTCCAGGGTTGATTTTGATTTACATTGGCGTCTGATCATTGATTTATCCTCAAAAACCCAACCTCATTGGTAATGAACCAACTCCGTGAATGTCCGCTCTGGCACAGAGCGACATTTTTTGATCTCCCCCCTGAAATCTAACCTTCGTCATGTCACGTTTTTGGGTTTCTAAAATTTTACCTTCGCGTTTTTc                                                    >  1:625002/1‑251 (MQ=255)                                                                                                                                                                                        aaaCACACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGAGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGc                                               >  2:562044/1‑251 (MQ=255)                                                                                                                                                                                          aCGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCg                                             <  1:41265/251‑1 (MQ=255)                                                                                                                                                                                            gCACACTTATTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATACCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCAAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCTCCCCTGAAAGCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGGTTTTCGTTGCCGTa                                           <  2:242875/251‑1 (MQ=255)                                                                                                                                                                                                 atttttAGAAAAAAGGATGGGCAGAAACGGGAGTAGCAATTACTGTCCAGGGTGGCATTTGATGTAAGTTGGCGTATGATCATAGAGTTATCCTCAAAAGCCAAACCTCATTGGTAATGAACCAGCTACGTGAATGTCCGATCAGGCACAGAGCGAAATGTTTTGATCTCCACACTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTATTCGTTGTCGTAAGGGt                                      <  2:352905/250‑1 (MQ=255)                                                                                                                                                                                                     ttAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCACAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTTATCTCCCACCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAAGTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTtat                                   <  2:506307/250‑1 (MQ=255)                                                                                                                                                                                                            aaTCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGCGCGAAATTTTTTGATCTCCCCCCTGAAATCTACACTTAGTCATGTCACGTTTTTGGGTTTCTACAATTTTAACTTAGCGTTTTTTGTTGCCGTAAGGGTTATACAGaaa                            >  2:228845/1‑250 (MQ=255)                                                                                                                                                                                                             aTCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTGCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATg                          <  1:512415/251‑1 (MQ=255)                                                                                                                                                                                                               cGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTGTCGTTGCCGTAAGGGTTATACAGAAATGTc                        <  1:603999/251‑1 (MQ=255)                                                                                                                                                                                                                         aaaCTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGACATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAACGGTTATACAGAAATGTCCGTTCAGc                >  2:455880/1‑249 (MQ=255)                                                                                                                                                                                                                          aaCTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGc                >  1:419230/1‑248 (MQ=255)                                                                                                                                                                                                                              ggATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTc         >  1:162158/1‑251 (MQ=255)                                                                                                                                                                                                                              ggATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTc         <  1:330728/251‑1 (MQ=255)                                                                                                                                                                                                                                     cAATTCCGTTCCAGGGTTGCGTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGACCCAGCTCCGTGAATGTCCGCTCTTGCACAGAGCGAAATTTTTTGATCTCCCACCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAattg  <  2:370756/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              GCGCTTTCCCTCTGGCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTG  >  NC_000913/4509997‑4510475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑