Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,489,016 C→T E350K (GAA→AAA)  yjgR ← DUF853 domain‑containing protein YjgR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,489,0160CT97.0% 205.1 / ‑2.6 67E350K (GAA→AAA) yjgRDUF853 domain‑containing protein YjgR
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (30/34);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

GCTGCTCGGTACTGGCCTGAAAGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAG‑AGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTCGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGC  >  NC_000913/4488770‑4489262
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      
gctgctCGGTACTGGCCTGAAAGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAG‑AGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGt                                                                                                                                                                                                                                                     <  1:3053/250‑1 (MQ=255)
          aCTGGCCTGAAAGCCTTTTTGCAGCATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATCCACCGGAG‑AGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGAACGCTCCCCCACAGAAGGGCTTCCTTTCGCCTCCAGCAACGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATA‑GCCTTTTTGGTATCAAACGCCg                                                                                                                                                                                                                                          >  1:27335/1‑251 (MQ=255)
           cTGGCCTGAAAGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAG‑AGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGAATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCgg                                                                                                                                                                                                                                         >  1:622017/1‑251 (MQ=255)
              cccggAAACCCTTTTTGCAACGTCTTCTAGGCGGATTCCCGGACCATCACACCCTCATTGTTGCCATACCCCGGAG‑AGTGATTAATCAAGCCAATACGCTCATCCTCCGTCACCGGCCCCATCCGCGAACAATGCGCGA‑TCACCATCGCACGCTCCACCACAGAACGGCTTCCTTTCGCACCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGAtt                                                                                                                                                                                                                                      <  2:565003/247‑1 (MQ=255)
                               cAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACAACGGAG‑AGAGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGAGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGGAAAGAGATCAACGACTCGCCGGTGCCCAGTTCCTGAATC‑GACTTTTTGGTATCAAACGCCGGAGTAGCCCGCATGGTTTgcg                                                                                                                                                                                                                     >  1:180808/1‑251 (MQ=255)
                                    tctcATAGGCGGATTCCCGGTACACCTCATCCTCATATTTGCCATACACCGGAG‑CGTGATTAACCAAGCCATTACGCTCATCTTACGTCACCGGCCCCATCCGCGCACCAAGCGCGA‑TCCCCATCGCACGCCCCACCACAGAAGGGCATCCTTTCGCCTCCTGAAACGAGCTCAACGCCTCGCCGGCGCCCAGTTCCTGAATC‑GACTTTTTGGTATCAAACGCCGGATTGGCCCGCacgggatgccccact                                                                                                                                                                                                                >  2:275769/1‑237 (MQ=255)
                                         gagccggctcccaggcccccctcatcctcatATTTCCCATACACCGGAT‑AGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCTATCAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCTCATCCAGAAAAGAGATCAACGCCTCGCCTGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCTCATGGTTTGCGCCGCTGCCtt                                                                                                                                                                                                           <  2:156755/232‑1 (MQ=255)
                                           ggCGGATTCCCTTCCCACCTCAGCCTCATATTTTCCATACGCCTGTGTACT‑ATTAATCAAGCCACTACGCTCATCTCCCGTCCCCGGCCCCATCCGCGAACAAGTCGCGA‑TCACCATCGCACGCTCCACCACAGAATGGCTTCCTTTCGCATCCAGAAAATAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCTCTGCCTTCa                                                                                                                                                                                                         <  2:1134/251‑1 (MQ=255)
                                           ggCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAG‑AGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTc                                                                                                                                                                                                          >  1:315775/1‑250 (MQ=255)
                                              ggATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAG‑AGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGccc                                                                                                                                                                                                                                 >  2:203200/1‑224 (MQ=255)
                                              ggATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAG‑AGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGccc                                                                                                                                                                                                                                 <  1:203200/224‑1 (MQ=255)
                                                    ccGGTCCACCTCATCCTCATATTTGCCATACACCGGAG‑AGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCACCGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCCCTGCTTTCt                                                                                                                                                                                                >  2:470108/1‑251 (MQ=255)
                                                     cgggccACCTTATCCTCACATTTGCCATACACCGGAG‑AGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTGCACTGCTTTCTg                                                                                                                                                                                               <  2:339211/247‑1 (MQ=255)
                                                         ccACCTCATCCTCATATTTGCCATACACCGGAG‑AGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCATTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCt                                                                                                                                                                                           >  1:384464/1‑251 (MQ=255)
                                                          cACCTCATCCTCATATTTGCCATACACCGGAG‑AGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCtt                                                                                                                                                                                          <  1:85185/251‑1 (MQ=255)
                                                             ctcatcctcatATTTGCCATACACCGGAG‑AGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTg                                                                                                                                                                                       >  1:566537/1‑251 (MQ=255)
                                                              tcatcctcagATTCGCCCTCCACCGTAA‑AGTGATTAATCATGCCATTGCGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCGCAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGTTGCCCCGTTCCTGAATC‑GCCGTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTTCGCCGCTGCCTTCACGGCTTTCTGATCttgt                                                                                                                                                                                        <  2:661872/249‑3 (MQ=255)
                                                               cctcctcatATTTGCCATACACCGGAG‑AGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTg                                                                                                                                                                                       <  1:194464/247‑1 (MQ=255)
                                                                           tGCCATACCCCGGAC‑ATTGATGACTCAAGCCCTTACGCTCATCTTCCGTCCCCGGCCCCATCCGCGAACAAGGCGCGACTC‑CCATCGCACGCGCCCCCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCTAACGCCGGATTGGGCCGCATGGTTTGCGCCGCGGCCTTCACTGCTTTCTGATCTTTTTGCTTAAAAGCCCg                                                                                                                                                                          <  2:119420/250‑1 (MQ=255)
                                                                           tGCCATACACCGGGG‑ATTTGTTAAACAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTTCGCCGCt                                                                                                                                                                                                                <  2:244470/212‑1 (MQ=255)
                                                                           tGCCATACACCGGAG‑AGTGATTAATCACGCCATTAAGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGCAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCt                                                                                                                                                                                                                >  1:244470/1‑212 (MQ=255)
                                                                            gCCATACACCGGAG‑AGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTCGGTATCAAAc                                                                                                                                                                                                                                              >  1:9104/1‑181 (MQ=255)
                                                                            cccATACACCGGAG‑AGCGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTCGGTATCAAAc                                                                                                                                                                                                                                              <  2:9104/180‑1 (MQ=255)
                                                                               aTACACCGGAG‑AGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAg                                                                                                                                                                     <  1:466600/251‑1 (MQ=255)
                                                                                 accccGGAG‑AGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCa                                                                                                                                                                   <  1:249927/248‑1 (MQ=255)
                                                                                   acCGGAG‑AGTGATTAATCAAGCCATTACGCTCATCTTCAGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATg                                                                                                                                                                 >  1:559040/1‑251 (MQ=255)
                                                                                     cGGAG‑AGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAACAGCCCGCAAAGCATGtt                                                                                                                                                               >  1:539971/1‑251 (MQ=255)
                                                                                            gTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAAcgc                                                                                                                                                         >  1:32650/1‑251 (MQ=255)
                                                                                              gATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAAcgcg                                                                                                                                                        >  2:224380/1‑250 (MQ=255)
                                                                                                  aaTCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTAc                                                                                                                                                   >  1:153221/1‑251 (MQ=255)
                                                                                                   aTCAAGCCATTACGCTCATCGTCCTTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCAGCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTAcc                                                                                                                                                  <  1:472301/251‑1 (MQ=255)
                                                                                                           tttccGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCACACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCAATGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGATCTGcc                                                                                                                                          >  2:362733/5‑251 (MQ=255)
                                                                                                           aTTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAAcg                                                                                                                                                          <  2:144833/235‑1 (MQ=255)
                                                                                                           aTTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAAcg                                                                                                                                                          >  1:144833/1‑235 (MQ=255)
                                                                                                               cGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTg                                                                                                                                                                                       >  1:450133/1‑202 (MQ=255)
                                                                                                                      cTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGCGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTCGCGCCGCTGCCTTCACTGCTTTCTGCTCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCTCGCACAtt                                                                                                                                >  2:135176/1‑250 (MQ=255)
                                                                                                                                 ggCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGCAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGGGCTGCCCGAGAACATTCTCCGGAATATc                                                                                                                    >  2:272773/1‑251 (MQ=255)
                                                                                                                                       agccGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACgg                                                                                                              <  2:32650/249‑1 (MQ=255)
                                                                                                                                       aTCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCt                                                                                                                                                                                                            <  2:524756/157‑1 (MQ=255)
                                                                                                                                       aTCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCt                                                                                                                                                                                                            >  1:524756/1‑157 (MQ=255)
                                                                                                                                         ccGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGt                                                                                                            <  1:272773/251‑1 (MQ=255)
                                                                                                                                           gcgAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGttt                                                                                                          <  2:229490/251‑1 (MQ=255)
                                                                                                                                             gAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATATTTTGGCGTAAAAGCCCGCAAAGCATGTTGACCGCGATTACCGAGCTGCCCGAGCACATTCTCCGGACTATCAGCCGGGTTTTg                                                                                                        >  2:206517/1‑251 (MQ=255)
                                                                                                                                                 aaGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGa                                                                                                     >  1:236919/1‑250 (MQ=255)
                                                                                                                                                             cccATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGAc                                                                                         <  1:224380/250‑1 (MQ=255)
                                                                                                                                                                      cGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTGTGCGCCGCTGCCTTCACTGCTGTCTGAGCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATGACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGcc                                                                                <  1:615705/251‑1 (MQ=255)
                                                                                                                                                                        cTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATcc                                                                                                                            <  1:486195/205‑1 (MQ=255)
                                                                                                                                                                          ccaccaCAGAAGGGTGTCCTTTCGCATCCATAAAAGAGATCAACGCCTCGCCGGTGCCCATTTCCTGAATC‑GCCTTTTTGGTATCAAACTCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTCCACTGCTTGCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTGTTGCGAAACTAACCAGACGCCCTCGccgt                                                                              <  2:908/249‑3 (MQ=255)
                                                                                                                                                                               acaGAAGGGCTTCCTGTCGCAGTCAGAAAAGAGATCAACGCCGCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATAACAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCg                                                                       <  2:286185/251‑1 (MQ=255)
                                                                                                                                                                                    aGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATaa                                                                  <  1:54453/251‑1 (MQ=255)
                                                                                                                                                                                     gggCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCAGGTGCCAAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTAGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATa                                                                   >  1:244082/1‑249 (MQ=255)
                                                                                                                                                                                      ggCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAACGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGCATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGAGAATa                                                                   >  2:28681/1‑248 (MQ=255)
                                                                                                                                                                                                 gCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTg                                                     <  1:679435/251‑1 (MQ=255)
                                                                                                                                                                                                       aGAAAAGAGATCAACGCCTCGCCGGAGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAAt                                               >  1:219413/1‑251 (MQ=255)
                                                                                                                                                                                                           aaGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCtt                                            >  1:480581/1‑250 (MQ=255)
                                                                                                                                                                                                           aaGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTa                                           <  1:28681/251‑1 (MQ=255)
                                                                                                                                                                                                               gaTCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATcc                                                                                                                            >  1:76808/1‑166 (MQ=255)
                                                                                                                                                                                                               gaTCAACGCCTCGCCGGTGCCCAGTTCCGTAATC‑GCCTTTGTGGTATCAAACGCCGGAGTGTCCCGCACGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCGTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCca                                       <  2:206983/251‑1 (MQ=255)
                                                                                                                                                                                                               gaTCAACGCCTCGCCGGTGCCCAGGTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTGTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGATCACATTATcc                                                                                                                            <  2:76808/166‑1 (MQ=255)
                                                                                                                                                                                                                  cAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATATTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCcagca                                    <  1:206517/251‑1 (MQ=255)
                                                                                                                                                                                                                     cGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTa                                 <  1:470108/251‑1 (MQ=255)
                                                                                                                                                                                                                        cTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTTGGTATCAAACGCAGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGATTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGGTTTGCGAAACGAACCAGACGCCCACGCATTTTGAGCGAATAAGCCTTATCACCTGCCCAATCTTATCCAGCAGCACCt                              >  2:333954/1‑251 (MQ=255)
                                                                                                                                                                                                                                   ccAGTTCCTGAATCTGCGTTTT‑GACATCAACCGCCGGATTGGCCCGCATGGTGTGCGCCGCTGCCTTCACTGCTTTCGTATCGTTGGGCGGAAAAGCCCGCAAATCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGATACGATCCAGACGCCCACGCCGTTTGCGCGAATAAGCCTTATCCCCTGCTCAATCTTATCCAGCAGTCACTGCggtgtagcg                   <  2:39181/251‑8 (MQ=255)
                                                                                                                                                                                                                                   ccAGTTCCTGAATC‑GCCGTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCGGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCg                   <  1:601789/251‑1 (MQ=255)
                                                                                                                                                                                                                                            gAATC‑GCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAAcag           <  1:625275/250‑1 (MQ=255)
                                                                                                                                                                                                                                                  gCCTTTTTGGTATCAAACGCCGGAGTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAAGCTTAGCCAGCAGTACCTGCGGGGGATCGTTAAACAGCAGAtg     <  1:179651/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     ttttTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAATACCTGCGGGGCATCGTTAAACAGCAGAtgggc  >  2:411788/1‑248 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      
GCTGCTCGGTACTGGCCTGAAAGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAG‑AGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGA‑TCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATC‑GCCTTTTCGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGC  >  NC_000913/4488770‑4489262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑