Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,475,493	T→C	F19F (TTT→TTC)	yjgL →	protein YjgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,475,493	0	T	C	100.0%	106.8 / NA	34	F19F (TTT→TTC)	yjgL	protein YjgL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/19);  total (15/19)

CAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGAT  >  NC_000913/4475271‑4475715                                                                                                                                                                                                                               |                                                                                                                                                                                                                               cAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGATTACGGCGAGTTTGTGGAAAGAGCGATTTTTGATATTTTTTTGTGAGTAAAATTTGAAATGCTTAGACGTTCTTATTCACTAAAGGCGTTCGTAATGAGCAAAATATCAGATTTAAATTAATTTCAACACATTACCTTAGCCGACAATTTCAAACAAAAAAGGGAAGTTTTAAATACCt                                                                                                                                                                                                    >  2:359043/1‑251 (MQ=255)    cccAGGTTCGCAAAGCAGTAGTGCAGATTCTTATGGCTTGATGTCTTTTCGCTGTAAATATCACAAGGTTTACGGCGAGTTTGTGAAAATAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCTCTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATGAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGc                                                                                                                                                                                                 <  2:501770/251‑1 (MQ=255)       aGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAACGTTTTTTGATATTTTTTTGTGAGGAAAATTTGTATAGCTTAGCCGTTCTTATTTAATCAAGGTGTTCCTAATGAGCAAAATATCAGATTTAATTTATTTTCAAAAAATTACATTAGCAGCAAATTTCAAACAAAACAGTGAACTTTTAAATACCTGGCgtg                                                                                                                                                                                              >  2:204169/1‑251 (MQ=255)        ggTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCgtgt                                                                                                                                                                                             <  1:474787/251‑1 (MQ=255)               aaaGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGgaatg                                                                                                                                                                                      <  1:186669/251‑1 (MQ=255)                        gtGAAGATGCTGATGGCGTAATGACTTTTCGACGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGAGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTTATGCTTAGACGTTCTTATTCACTCAAGTAGTTCGTCATGATCAAAATATTAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGAGACGTTTTAAATACATGGCGTGTTGAAATAAATGAtttt                                                                                                                                                                             >  2:621353/1‑251 (MQ=255)                                  tGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTAttt                                                                                                                                                                   >  1:583148/1‑250 (MQ=255)                                       gCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCggg                                                                                                                                                              >  1:599175/1‑251 (MQ=255)                                                      gCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGa                                                                                                                                               <  1:336453/251‑1 (MQ=255)                                                           aaaTAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGa                                                                                                                                           <  1:5493/250‑1 (MQ=255)                                                                     aGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATAttcttgct                                                                                                                                <  2:231666/251‑4 (MQ=255)                                                                       gTTTACGGCGAATTGGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCTAGGAGGTCGTCACGAGCAAAATATCAGATTTAAATTATTCTCAACTCATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCGGGCGTGTTGTAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATGCTTTCTcc                                                                                                                              <  2:2938/251‑1 (MQ=255)                                                                                    ttGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCAttttt                                                                                                                  >  2:115599/1‑250 (MQ=255)                                                                                            agagCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGttttt                                                                                                         <  1:376608/251‑1 (MQ=255)                                                                                                    ttttGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAg                                                                                                  <  2:19146/250‑1 (MQ=255)                                                                                                                      ggTAAAATTTGGAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGAGTTAAATTATGCGCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGGGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAAGAATAGTCTCTCTCCTTTAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTTGGTTAtg                                                                               <  2:652391/250‑1 (MQ=255)                                                                                                                         aaaaTTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTgg                                                                            >  1:305096/1‑251 (MQ=255)                                                                                                                              ttGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGAttt                                                                        <  1:512205/250‑1 (MQ=255)                                                                                                                              ttGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTATAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCATGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTTGCAAAGATATTTACCCTGGGTTATGTGGAtgt                                                                        <  2:485425/250‑3 (MQ=255)                                                                                                                                             gTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAAc                                                        >  1:577201/1‑251 (MQ=255)                                                                                                                                             gTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGACGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAAc                                                        >  1:68017/1‑251 (MQ=255)                                                                                                                                              ttCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACg                                                       >  1:258689/1‑251 (MQ=255)                                                                                                                                                              gAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTAAACACATTACATTAGCCGACCATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCACGTATTGCACGGGGGCAGGATAACGGAAGGAATATTCTTTATCCTGGAACATTTTTAGAGTTTTTGGCATAGATATTTACCCTGGTTTATGTTGATTTTAGTAAACGCTCCAACACAGAGGGTAGAAattt                                       >  2:107792/1‑249 (MQ=255)                                                                                                                                                               tgTTCGTCATGAGAAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGATGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGTGCATTTTTGGCGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGTTTTTAGCAAACGCTCAACCGAAGAGGGTAGAGatat                                       >  2:290345/2‑250 (MQ=255)                                                                                                                                                                  tCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATatgat                                    <  1:359043/250‑1 (MQ=255)                                                                                                                                                                    gTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTAt                                                                                >  1:52882/1‑204 (MQ=255)                                                                                                                                                                    gTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTAt                                                                                <  2:52882/204‑1 (MQ=255)                                                                                                                                                                      cATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTc                               <  2:577201/251‑1 (MQ=255)                                                                                                                                                                         gagaaaaaTATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATTATTTTGCCCGTATTGCCGTGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCata                            <  2:509280/247‑1 (MQ=255)                                                                                                                                                                         gAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCat                             >  1:556152/1‑250 (MQ=255)                                                                                                                                                                                    cAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCa                 <  1:290345/251‑1 (MQ=255)                                                                                                                                                                                     aGATTTAAATTATTCTCAACACATTACATTAGCCGACAAGTTCAATCAAAAAAGTGAAGTTTTAAATCCCTGGCGTGTTGGAATGAATGATGTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTTGAGCATTTTTATAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCAt                <  1:658433/251‑1 (MQ=255)                                                                                                                                                                                            aaTTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTc         <  1:567313/251‑1 (MQ=255)                                                                                                                                                                                                    tcAACACATTACATTAGCCGACAATTTCAAACAACAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGCTGGCTCATATTAAGTCCTCATCTTATTCTAAAGAt  >  2:596131/1‑250 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                               CAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGAT  >  NC_000913/4475271‑4475715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑