Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,344,935	G→A	T208T (ACC→ACT)	yjdF ←	DUF2238 domain‑containing inner membrane protein YjdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,344,935	0	G	A	100.0%	203.7 / NA	63	T208T (ACC→ACT)	yjdF	DUF2238 domain‑containing inner membrane protein YjdF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/29);  total (34/29)

AGGATCCAGATCCATCTGCTGGATAAATATCGCAAAGTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCGGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCG  >  NC_000913/4344692‑4345180                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       aGGATCCAGATCCATCTGCTGGATAAATATCGCAAAGTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATc                                                                                                                                                                                                                                                  >  1:132550/1‑250 (MQ=255) aGGATCCAGATCCATCTGCTGGATAAATATCGCAAAGTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATc                                                                                                                                                                                                                                                  >  1:650402/1‑250 (MQ=255) aGGATCCAGATCCATCTGCTGGATAAATATCGCAAAGTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAAAGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATc                                                                                                                                                                                                                                                  >  2:79216/1‑250 (MQ=255)   gatccagacccaTCTGCTGGATAAATATCGCAAAGTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAg                                                                                                                                                                                                                                               <  1:154042/251‑1 (MQ=255)         gatccaTCTGCTGGATAAATATCGCAAAGTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCAGGCATCCACAAAGCGCAAGTTGTTATCCAGTTATCAAACCaa                                                                                                                                                                                                                                           >  2:367450/1‑249 (MQ=255)             caTCTGCGGGATAAATATCGCAAAGTACCGCCCGAGCCTGGTCATGCTGATATTCCGGTCGGGGCAGTGAGTGAGGTTAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGcc                                                                                                                                                                                                                                     <  1:358474/251‑1 (MQ=255)                   ctgGATAAATATCGCAAAGTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCt                                                                                                                                                                                                                               >  1:531176/1‑251 (MQ=255)                        aaaaTACCGAAAAGTTCCGCCCGAGCCGTTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTTACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTggcgg                                                                                                                                                                                                                          <  2:58135/250‑1 (MQ=255)                           atatCGCAAAGTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGACTTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCa                                                                                                                                                                                                                        >  1:122879/1‑250 (MQ=255)                             atCGCAAAGTACCGCCTGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAAt                                                                                                                                                                                                                      <  1:674658/250‑1 (MQ=255)                                 aaaaGTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAag                                                                                                                                                                                                                 <  1:534195/250‑1 (MQ=255)                                     gTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAagcg                                                                                                                                                                                                               >  1:355429/1‑249 (MQ=255)                                     gTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAagcg                                                                                                                                                                                                               >  1:473456/1‑249 (MQ=255)                                              gatccttttccttcttatcttCCGGTCGGTGCAGTGCGTGATGTTAAAGCCTGACGTGGACAATAACTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAat                                                                                                                                                                                                     >  1:352819/20‑250 (MQ=255)                                              gAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATTGGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGa                                                                                                                                                                                                                    <  2:519583/235‑1 (MQ=255)                                              gAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGa                                                                                                                                                                                                                    >  1:519583/1‑235 (MQ=255)                                                             gATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTTACGAAAATGGCCTTATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTaa                                                                                                                                                                                       <  1:607426/249‑1 (MQ=255)                                                                atTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATgcg                                                                                                                                                                                   <  1:137804/250‑1 (MQ=255)                                                                 ttCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATgcccc                                                                                                                                                                                 >  2:339606/1‑248 (MQ=255)                                                                         ggtGCAGTGAGTGATTTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAgcg                                                                                                                                                                         <  1:283574/251‑1 (MQ=255)                                                                         ggtGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAgcgagcga                                                                                                                                                                                                          <  1:425964/218‑1 (MQ=255)                                                                            gcaggggttgaTGTGAAAGCCTTACTTGGACAATAGTTAACGAAAAGGGCCTGTTGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAgcgcg                                                                                                                                                                       <  2:354355/242‑1 (MQ=255)                                                                                          gAAAGCCGTACGTGGACAATAGCTAACGAAAAGGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGa                                                                                                                                                         <  2:650402/250‑1 (MQ=255)                                                                                           aaagaatGACGTCGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTATTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGAATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGGTATCAAGCCAAAGCGCCGTAGCTGACAGCAATGAAAGCGAGAGAGGACTATAAACGTCTGTAATGCGCAAGGCAGCGCGACGAACAAATCggctt                                                                                                                                                       >  2:577087/7‑248 (MQ=255)                                                                                             aGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGTTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGc                                                                                                                                                     <  1:450553/251‑1 (MQ=255)                                                                                                         aCAATAGCTAACTAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCTCCGTAGCTGGCGGCAAt                                                                                                                                                                                                                      <  2:674310/174‑1 (MQ=255)                                                                                                         aCAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAAt                                                                                                                                                                                                                      >  1:674310/1‑174 (MQ=255)                                                                                                           aaTAGCTAACGAAAAGTGCCTGATGCGATATGTTTATCAGGCCACGTTTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGGTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTc                                                                                                                                       <  2:262291/251‑1 (MQ=255)                                                                                                           aaTAGCTAACGAAAAGGGCTTGATTCTATATGTTTATCAGGCCACGTCTTAATGTAATGCTGTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCTCCGTCGGTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCTTGTCCCACTGGTc                                                                                                                                       <  2:355429/251‑1 (MQ=255)                                                                                                                cTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCACGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGcccc                                                                                                                                  >  2:98417/1‑250 (MQ=255)                                                                                                                cTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCt                                                                                                                                  >  1:661733/1‑251 (MQ=255)                                                                                                                 tAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTg                                                                                                                                 >  1:77324/1‑251 (MQ=255)                                                                                                                  aaCGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTgg                                                                                                                                <  1:170057/251‑1 (MQ=255)                                                                                                                    cGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGt                                                                                                                              <  1:534143/251‑1 (MQ=255)                                                                                                                                     gATATGTTTAGCAGGCCACGTATTAATGTAATGCTTTGGATTTGCAAATTTTGAAGACCGGATAAGGCATTCACGCCGCATCCGTCGTCAACAAAGCGCAAGTTGTTATACAGATATCAAGCCCACGCGCCGTCGCTGGCGGCAATGAAAGCGAGCGAGGCATATCACCATAGTTAATGCGCCAAGCAGCGCGCAGAACATATCAGGTTGAGTGTCCCACTGGCCGCCCTGGGTGCCGGAAAAATAATCCg                                                                                                             >  2:400954/1‑251 (MQ=255)                                                                                                                                                     cACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACAGGATAAGGAATTAACTCCGCATCCGTCATCACAAAAGAGCAAAGTGTTATCAAGTTATCAAGACAAAGCACCGTAGCTAGCGACACTGAAAGCGAGCGAAGAATATCACCGCCGTTAATGCGCCGAGCCGCGCGCAGAACGTACCGGCTTGCGTATCCCAATCGGCGCCACGGGTGCCGAATCAAGCATCCGCTcccagcacccttgc                                                                                             >  2:114814/1‑240 (MQ=255)                                                                                                                                                          cTTAATGTAATGCTTTGGATTTGCAAATTTTGCAGACCGGATACGGTATTCACGCCGCATCCGGAATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCAAGGCATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGAATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCATGACCACAT‑GCCAgcg                                                                                       >  2:68285/1‑251 (MQ=255)                                                                                                                                                             aatgtattgCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTg                                                                                    <  1:440870/251‑1 (MQ=255)                                                                                                                                                                     gCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTGCccacca                                                                             <  1:79216/250‑1 (MQ=255)                                                                                                                                                                                    aTTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGTAAGTTGTTATCCATTTATCAAGCCAAATCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCACGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTTGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGTCC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATAc                                                             <  1:222448/251‑1 (MQ=255)                                                                                                                                                                                    aTTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATCTCGGATTGCGTGTCACACTAGTCGCCCTGGGTGCCGAGCAAATCATCCGCTCCCTGACA‑CATTGCCAGCGCTGCCCACCCCTCGATTAATTCATAc                                                             >  2:110293/1‑251 (MQ=255)                                                                                                                                                                                           aGACCGGATGAGGAATTAACGCCGCATCCGGAATCCCCAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAACGCGCCATAGCTAGCGGCAATGAAAACGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACCTCTCGCATTGCGTGTCCCACTGGCCCCCCTAGGTGCCGCACAACTCTTCCGCTCCCTCACC‑CACTGCCACCACTGCCCACCCCTCGATTCATTCATACATCccgc                                                      >  2:672578/1‑251 (MQ=255)                                                                                                                                                                                           aGACCGGATAAGGAATTAACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCCAAGCGCCGTAGCTGGCGGCAATGAAAGCGCGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGAGCGCAGAACATCTCGGATTGCGTGTCCACCTGGTCGCCCTGGGTGCCGAGAAACTCATCCGCTCCCTGCCC‑CATTGCCAGCGCTGCCCCCCACTCGATTAATTCATACATCgcgc                                                      >  2:660694/1‑251 (MQ=255)                                                                                                                                                                                             aCCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCtt                                                    <  1:168250/251‑1 (MQ=255)                                                                                                                                                                                                ggATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATc                                                 >  1:491596/1‑251 (MQ=255)                                                                                                                                                                                                 gaaaaGGAATTCACGCCGCAACCGGCAACAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCg                                                <  2:46419/248‑1 (MQ=255)                                                                                                                                                                                                  aTAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCACTCCCTGACC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGc                                               >  1:503802/1‑251 (MQ=255)                                                                                                                                                                                                         aTTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGGTATCCAGTTAGCAAGCCGAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGATGAATATCACCGTCGTTAATGCGCCAAGCAGCGCTCAGAACATATCTGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTGCCCACCACTCGAGTAATTCATACATCGCGCTTATCGCCAGCGcc                                        <  2:99015/251‑1 (MQ=255)                                                                                                                                                                                                            cACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCAcc                                     >  2:314019/1‑250 (MQ=255)                                                                                                                                                                                                            cACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACg                                     >  1:526309/1‑251 (MQ=255)                                                                                                                                                                                                               gccgcATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCCCTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACgcag                                  <  1:718/251‑1 (MQ=255)                                                                                                                                                                                                                ccgcATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACgcagc                                 >  1:619756/1‑251 (MQ=255)                                                                                                                                                                                                                   caacctgcacaaacaaAGCGCACGTTGTTATCAAGTTATCAAGCCAAAGCGCCGTAGCGGTCGGCAATGAAAGTGAGCGAGGAATAGCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCTTGTCCCACTGGGCGCCCTGGGTGCCGAGAAAATCATCCGCGTCCTGACC‑CACTGCCAGCGCTGCCCACCTCTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACgcagca                                <  2:448584/238‑1 (MQ=255)                                                                                                                                                                                                                      ccGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGAcc                            >  1:373207/1‑250 (MQ=255)                                                                                                                                                                                                                               acaaAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGccc                  >  1:428465/1‑250 (MQ=255)                                                                                                                                                                                                                                   aGCGCAAGTTGTTATCCAGTTTTCAAGCCAAAGCGCCGTACCTGGCGGCAATGAAACCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCTGCGCGCTGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTTCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTGATCGCCAGCGCCACTCAGCAGACCAGGAACGCCACCAt              <  2:428465/251‑1 (MQ=255)                                                                                                                                                                                                                                   aGCGCAAGTTGTTATCCAGTTATCAAGCCAACGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTg                                                                                             >  1:507356/1‑172 (MQ=255)                                                                                                                                                                                                                                      gcAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGTCCTGGGTGCCGAGAAACTCATCCGCTCCCTGCCC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCAtttt           >  1:321401/1‑251 (MQ=255)                                                                                                                                                                                                                                        aaGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTAc         >  1:371303/1‑251 (MQ=255)                                                                                                                                                                                                                                        aaGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTAc         >  1:185694/1‑251 (MQ=255)                                                                                                                                                                                                                                             gttATCCAGTTAGCAAGCCAAAGCGCCGTAGATGGAGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCTCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTcc     <  2:122879/250‑1 (MQ=255)                                                                                                                                                                                                                                              ttATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCcgc   >  1:61233/1‑251 (MQ=255)                                                                                                                                                                                                                                                aTCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCcgcg  <  1:182638/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       AGGATCCAGATCCATCTGCTGGATAAATATCGCAAAGTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCGGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACC‑CATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCG  >  NC_000913/4344692‑4345180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑