Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,320,416 G→A T83I (ACC→ATC)  phnI ← carbon‑phosphorus lyase core complex subunit PhnI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,320,4160GA95.0% 283.5 / ‑4.4 103T83I (ACC→ATC) phnIcarbon‑phosphorus lyase core complex subunit PhnI
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base A (48/47);  total (52/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

CGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGT‑CAGCGTCGG‑CGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGG‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAGCTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAAC  >  NC_000913/4320169‑4320657
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  
   cAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGG‑CAGCGTCGG‑CGCTTCGCCGTTTGCCAGCAGGGGAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGGAGGTTGGGCCAAGAAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGAAGCATCCCGGTGGGGGCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCCACGGGG‑TGCggg                                                                                                                                                                                                                                             >  1:253891/1‑250 (MQ=255)
            gcgcgCCAGCAGCCTGAAAACGGCCGGCGCCGGCTGCGTTTCGCTGGCGGCGGTGGT‑CAGCGTCGG‑CGCTTCGCCGTTTGCCAGCAGGGTAAAAGCGAGCAGGCGATGGGTG‑TAGC‑CGTAGGTGGGGCCAAGCAGCTGGCCGCTGGGAATGTCTTTATAAACGACGGATATACGGCGTTCGAGACGCATCCCGGTGGTGTCGATCGGCGC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCgca                                                                                                                                                                                                                                    <  2:668567/250‑1 (MQ=255)
                 ccggcagGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGT‑CAGCGTCGG‑CGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAgg                                                                                                                                                                                                                               <  1:237713/248‑1 (MQ=255)
                  cagcagGCTGAAAAGGTGGGGGGGCGGCTGTGGGTCGCTGTCGGCGG‑GGTGCGGCGTCGG‑CGCTTGGCCGTTGTCCAGCAGGGTAAAATCGAGCAGGCGTTGGGGG‑TAGT‑CGTAGGTTGGGCCAATCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGGTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGa                                                                                                                                                                                                                              <  2:629452/251‑1 (MQ=255)
                  cagcagGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGT‑CAGCGTCGG‑CGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGGGATACGGCGTTCGAGACGCATCCCGGGGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAgg                                                                                                                                                                                                                               >  1:645526/1‑250 (MQ=255)
                     aaggttGAAAGCGGGCGGCGACGGCTGCTTTCCGCTGTCGGCGGTGGT‑CAGCGTCGG‑CGCTTCGCCGTTGGCCTGCATGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CTTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGGGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGGGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGaaaa                                                                                                                                                                                                                           <  1:398131/246‑1 (MQ=255)
                         cTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGT‑CAGCGTCGG‑CGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGAGC‑TGCGGTAGGCGCGCAGCAGGAAAATCGc                                                                                                                                                                                                                       >  1:655676/1‑251 (MQ=255)
                               ccGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGG‑CAGCGTCGG‑CGCTTCGCCGGTTGCCAGCAGGGTAAAAGCGAGCAGGCGAGGGGGG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCTGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGGTGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCGTCGCCAACGTGA‑GGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAAc                                                                                                                                                                                                                 <  1:626789/250‑1 (MQ=255)
                                           ggCTGCTGTTCGCTGTCGGCGGTGGT‑CAGCGTCGG‑CGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCCGGAAAATCGCTTCAACGTTATCGCCGCt                                                                                                                                                                                                     >  1:186009/1‑251 (MQ=255)
                                           ggCTGCGGTTCGCTGTGGGCGGGGGTGTGGCG‑CGG‑CGCTCCGCCGTTTGCTAGCAGTGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CTTAGGTTGTGCCAAGCAGCTGGCCGCCGGGAATGTCTGTATAACCGCCGGAGATACGGCGTTCGAGCCGCATCCCGGTGGTGGCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCt                                                                                                                                                                                                     <  2:124333/251‑1 (MQ=255)
                                             ctgctgTTCGCTGTCGGCGGTGGT‑CAGCGTCGG‑CGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCCACGTTATCCCCGCTgg                                                                                                                                                                                                   >  1:356934/1‑251 (MQ=255)
                                                       gggTCGGGGGGGGT‑CAGCGTGGG‑CGCTTCGCGGGTTGCGAGAAGGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTGGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGGGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcag                                                                                                                                                                                         <  2:285569/249‑1 (MQ=255)
                                                       cTGTCGGCGGTGGT‑CAGCGTCGG‑CGCTTCGCCGTTTGCCAGCAGGGTAAAATCGCGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCCGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTc                                                                                                                                                                                           >  1:277501/1‑249 (MQ=255)
                                                         tgcggggggggt‑CTGCGTCGG‑CGCTTCGCCGTTGGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTGGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagc                                                                                                                                                                                        <  2:216720/241‑1 (MQ=255)
                                                          ttcgtcggttggcAGCGGCGG‑CGCTTCGCGGGTGGCCAGCAGGGAAAAATCGAGCAGCGGATGGGTG‑TGGT‑GGTAGGTGGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTAAAACCGACGGAGATACGGCGTGCGAGACGCACCCCGGTGGGGTCGAGCGGCTC‑GCGTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcag                                                                                                                                                                                         <  2:647540/237‑1 (MQ=255)
                                                             ggggtggt‑CCGCGCCGG‑CGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGTTGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GTTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgccag                                                                                                                                                                                   <  2:485641/249‑1 (MQ=255)
                                                             gcggTGGT‑CAG‑GTGGGGCGCTTCGCCGTTGGCCATCAGGGTAAAATCGAGCAGGCGAGGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAACCGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgccag                                                                                                                                                                                   <  2:169939/251‑1 (MQ=255)
                                                               ggcggt‑CAGCGTCGG‑CGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGACGCATCCCGTGGGTGTCGCGCGGCTC‑GCCTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGGTATCGCCGCTTGCCTGTTTcagcccccgcc                                                                                                                                                                                 >  2:183642/4‑244 (MQ=255)
                                                                      cAGCGTCGG‑CGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGCTGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCgccgcc                                                                                                                                                                            >  1:249286/1‑250 (MQ=255)
                                                                          gTCGG‑CGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GTTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTc                                                                                                                                                                       <  1:349540/251‑1 (MQ=255)
                                                                                 gcTTCGCCGGTTGCCAGGAGGGTAAAATCGAGCATGCGATGGGTG‑AAGT‑CGTAGGTTGGGCCCAGCGGCTGGCCGCCGGGCATGGCTTTATAAACGACGGAGCTACGGTGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCGC‑GCTTACC‑ACCAGCTTCGCCTACGTGA‑AGCGGGCGGCGCGCAGCAGGGAAATCGCTTATACGGTATAGCCGATGACCTGGTTcagagacagcccccaccggacgatatc                                                                                                                                                                 >  2:104269/1‑227 (MQ=255)
                                                                                   tgcGCCGTTTGCCAGCAGGGTAAAAGCGAGAAGGCGATGGGTG‑TAGT‑CGTAGGTGGGGCCAGGCAGCTGTCCGCGGGGAATGTCTTTATAAACGACGGAGCTACGGCGTTCGAGCCGCATCCCGGTGTTGTCGAGCGGCTC‑GTTTACC‑GCCAGCTTCGCCAACGGGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCg                                                                                                                                                                <  1:420719/248‑1 (MQ=255)
                                                                                        ggttggccggcgggcaaacaTCGGGCAGGCGATGGGTG‑‑AGTGCGTGGGTGGGGCAAAGCAGCGGGCGGCCGGGAATGTCTTTAAAAACGCCGGAGAGCCGGCGTTCGGGCCGCACCCGGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATg                                                                                                                                                          <  2:135409/232‑1 (MQ=255)
                                                                                        cGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATg                                                                                                                                                          <  1:202168/251‑1 (MQ=255)
                                                                                        cGTTGGCCAGCAGGGTAAAATCGAGCAGGCGAGGGGTGGTGGT‑‑GTAGGTTGGGCCAAGGAGCTGGCCGCCGGAAATTTCTTTAGAAACGACGGTGATACGGCGTTCGAGACGCTCCCCGGTGGGGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATg                                                                                                                                                          <  2:356934/251‑1 (MQ=255)
                                                                                           ttGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATgcc                                                                                                                                                        <  1:601599/250‑1 (MQ=255)
                                                                                            tgccggcggggTAAAATCGACCGGGGGATGGGTT‑GGGT‑CGTGGGTTGGGCCAGGCGGTGGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCTTCCCGGTGGTGTCGGGCGGCTC‑GCTTCCC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATgccg                                                                                                                                                       <  2:479945/242‑1 (MQ=255)
                                                                                              ccAGCAGGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATcgccg                                                                                                                                                                                                       <  2:541980/200‑1 (MQ=255)
                                                                                              ccAGCAGGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATcgccg                                                                                                                                                                                                       >  1:541980/1‑200 (MQ=255)
                                                                                                agcagGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGGTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCACAGCCGCCTTc                                                                                                                                                  >  2:118463/1‑251 (MQ=255)
                                                                                                 gcagGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCg                                                                                                                                                 >  1:56936/1‑251 (MQ=255)
                                                                                                 gcagGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCCTCAACGTTATcgc                                                                                                                                                                                                         >  1:547452/1‑195 (MQ=255)
                                                                                                 gcagGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCCTCAACGTTATcgc                                                                                                                                                                                                         <  2:547452/195‑1 (MQ=255)
                                                                                                  cagGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCCGCGCCGCCAGTTCGCGGTCGGCAAAGCCGCCTTCgg                                                                                                                                                >  2:136748/1‑251 (MQ=255)
                                                                                                    gggTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGtc                                                                                                                                              >  1:240590/1‑251 (MQ=255)
                                                                                                               aGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATc                                                                                                                                   >  1:419413/1‑251 (MQ=255)
                                                                                                               aGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGATGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGC‑TGCGGTAGGCGCGCAGCAGGAACATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCTGCCTTCggacctccagcggtc                                                                                                                                   >  2:199647/1‑238 (MQ=255)
                                                                                                                  aGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGCTACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTAc                                                                                                                                >  1:273557/1‑251 (MQ=255)
                                                                                                                   ggCGATGGGTG‑TAGT‑CGTAGTTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATCCGGCGTTCGAGCCGCTTCCCGGTGTTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTAcc                                                                                                                               <  1:516808/251‑1 (MQ=255)
                                                                                                                   cgtgtgtggggtggagtcggggggtGGGCCACGCAGCGCGCCCCCGGGAATGTCGTTATACACTGCGGGAATACGGCGTTCTAGACGCATCCCGGTGGTGTTGAGCGGCGC‑GCTTCCC‑GCCAGCTTCGCCAACGTGA‑GGCGGTAGGCGCGCAGCAGGAAAATCGCGTCAACGTTATCTCCGCTGGCCTGTTGCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTa                                                                                                                                 <  2:555641/227‑1 (MQ=255)
                                                                                                                    gCGAGGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACcg                                                                                                                              <  1:49646/251‑1 (MQ=255)
                                                                                                                         tggTG‑TAGT‑CGTAGGGTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCCTCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCCGCGGGAAAAACGCTTCAACGTTATCGCCGCTGGCCTGTTTCAACACCAGCACCGCCAGATCGCGGTCTACAATGCCGCCTTCGGACATCAAGCGAtatcacgcgagg                                                                                                                         >  2:507595/2‑240 (MQ=255)
                                                                                                                           ggttgtattggtgggtggggCCAAGCAGCTGTCCGCGGGTAATGTTTTTATAAACGACGGAGACTCGGCTTTGTATACTCATCCGGGGGGGGTCGTGCGGCCG‑GCTTCCC‑GCCAGCTGCGCAAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAACTCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAg                                                                                                                          <  2:638783/233‑1 (MQ=255)
                                                                                                                            tg‑tAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCCCGCGATCTACcgcgcgggtt                                                                                                                      >  2:140918/1‑245 (MQ=255)
                                                                                                                            tg‑tAGT‑CGTAGGTTGGGCCAAGAAGCTGGCCGCCGGGAATGTCTTTATAAAAGACGGAGATACGGCGGTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGCAAATCGCTTCCACGTTATCGACGCTGGCCTGTTTCAGCGCCAGCCCCGCCAGTACGCGGTCGGCAATGCCGCCTTCGGTCCTCACGCGGTCTACCGCGAggat                                                                                                                       >  2:315405/1‑248 (MQ=255)
                                                                                                                                 ggccggcgtgtGGGCCACGCATCTGCTCGCCGGAATTGTCTTTAAAACCGGCGGTGATGCGGCGTTCGAGCCGCACCCCGGTGGTGTCGA‑CGGCCCCGCTTCCC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCGTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTaaa                                                                                                                    <  2:269202/240‑2 (MQ=255)
                                                                                                                                     gTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTGCGAGCCGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAactgct                                                                                                               <  1:119596/251‑1 (MQ=255)
                                                                                                                                       gggTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGt                                                                                                             <  1:184920/250‑1 (MQ=255)
                                                                                                                                       aGGTGGGGCCAAGCAGCTGGCGGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTGCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAActgctg                                                                                                              <  1:415059/250‑1 (MQ=255)
                                                                                                                                              gCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCt                                                                                                      <  1:118463/251‑1 (MQ=255)
                                                                                                                                               ccAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGGGGTTAAACTGCTGGTCAATCTg                                                                                                     >  2:529789/1‑251 (MQ=255)
                                                                                                                                                cAAGCAGCTGGCTGCCGGGAAGGTTTTTATAAACGACGGAGATACGGCGTGCGAGACGCATTCCGGTGGTGTCGAGCGGCTG‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTgg                                                                                                    <  1:412102/251‑1 (MQ=255)
                                                                                                                                                cAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTGCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTgg                                                                                                    <  1:388147/251‑1 (MQ=255)
                                                                                                                                                     agcTGGCCGACGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGCCGCATCCCGGTGGGGTCGCGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCACAGTTATCGACGCTGGCCTGTTTCAGCGCCCGCTCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGGTAAACTGATGTTCAATCTGTGCGAc                                                                                               >  2:667454/1‑251 (MQ=255)
                                                                                                                                                       cTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGc                                                                                                  >  1:494351/1‑246 (MQ=255)
                                                                                                                                                        tGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCCACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCACTATGGTCGACGcc                                                                                            >  2:624654/1‑250 (MQ=255)
                                                                                                                                                              ccgGGAATGTTTTTATAAACGACGGAGATACGGCGTTCGAGACGCAGCCCGGGGGGTTCGAGCGGCTC‑GCTTACC‑GCCATCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCATCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTc                                                                                      <  1:395919/251‑1 (MQ=255)
                                                                                                                                                               cgGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCCTCCCGGTGGTGTCGAGCGGCAC‑GCTTACC‑GCCAGCTTCGCCACCGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAACGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGAACCACCGCGAGGTTAACCTGCTGTTCGATCTGGGCGACGCCCAGTTCg                                                                                     >  2:672979/1‑251 (MQ=255)
                                                                                                                                                                gggAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCgg                                                                                    >  1:292723/1‑251 (MQ=255)
                                                                                                                                                                      gTCTTTATAAACGACGGCGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGCGCGGCTC‑GCTTACC‑GACAGCTTCGCCAACGTGA‑TGCGGTCGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGACGCTGGCCTGTTTCAGCGCCAGCGCCGCCCGTTAGCGGTCGGCAAAGCCGCCTTCGGGCATCACGCGATCTCCCGCAGGGTTAAACTGCTGTTCCATCTGGGCGACGCACAATTAGGGCAAAt                                                                              >  2:674183/1‑251 (MQ=255)
                                                                                                                                                                            ataAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GATTACC‑GCCAGCTTCGCCAAAGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGGTTCAGCGCCAGCGCCGCCAGTTCGCGGTGGGCAATGCCGCCTTCGGTCAGAACGCGATCTACCGCGAGGTTAAACGGCTGTACAAACAGGGGGACGCTCAGTTCGGGCAAAtaggga                                                                         >  2:497467/1‑245 (MQ=255)
                                                                                                                                                                               aaCGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGGGTCGAGCGGCTC‑GCTTAAC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGGAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACGGCTGTTCAATCTGGGCGAAGCACAGTTCGGGCAAATCGGTATCGc                                                                     >  2:648164/1‑251 (MQ=255)
                                                                                                                                                                                 cgacggAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGccc                                                                   >  1:364145/4‑250 (MQ=255)
                                                                                                                                                                                 cgacggAGATACGGCGTTCGAGACGCACCCCGGGGGTGGCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCt                                                                   <  1:272377/248‑1 (MQ=255)
                                                                                                                                                                                   acggAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCg                                                                 >  1:369139/2‑251 (MQ=255)
                                                                                                                                                                                      gagatccgggggtCGAGACCCATCCCGGTGTGGTCGAGCGGCTC‑GCTAACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCg                                                              <  1:482219/239‑1 (MQ=255)
                                                                                                                                                                                        aatacgggggtctggaCGCTTCCGGGGGGTGGCGTGCGGTTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGGGCAGCAGGAAAAGCGCTTCAACGTTAGCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGta                                                            <  2:149057/237‑2 (MQ=255)
                                                                                                                                                                                         gctggggctcggggcccgttccgggtggtGCCGGGCGGCTC‑‑CTTGCCCGCCAGCTTCGCCACCGTGA‑TGCGGTAGGCGCGCAGCAGGAAAACCGCTTCAACTTTATCGCCGCTGTCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCg                                                           <  2:511566/228‑1 (MQ=255)
                                                                                                                                                                                         aTACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCg                                                           >  1:88949/1‑251 (MQ=255)
                                                                                                                                                                                          tACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCgg                                                          >  1:654231/1‑251 (MQ=255)
                                                                                                                                                                                           aCGGCGTCCGAGCCGCATCCCGGTGGTGTCGAGCGGCCC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCGGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGc                                                         <  1:191496/251‑1 (MQ=255)
                                                                                                                                                                                             ggCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGctc                                                       >  1:310193/1‑251 (MQ=255)
                                                                                                                                                                                                ggtCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAACTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATTTGGGCGACGCTCAGTTCGTGACAACAGGTATCGGCTCGGCGTCGGcacagt                                                    >  2:385872/3‑246 (MQ=255)
                                                                                                                                                                                                gTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGctctct                                                    >  1:82135/1‑251 (MQ=255)
                                                                                                                                                                                                    gaaactcATCCCGGGGGTGTCGGGCGGCTC‑GCTTCAC‑GCCAGCTGCGCCAACGGGA‑TGCGGTAGGCGCGCAGCAGGAAAAACGCTTCAACGTTATCGCCGCTGGACTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCa                                                <  1:657187/245‑1 (MQ=255)
                                                                                                                                                                                                          cATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCCGTTCGGGCAACTCGGTATCCCCTCGGCGTCGGCTCCCTTGCAGGGCGt                                          >  2:573633/1‑251 (MQ=255)
                                                                                                                                                                                                             cccGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTggg                                       >  1:333444/1‑251 (MQ=255)
                                                                                                                                                                                                                    gtgtCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTc                                >  1:650726/1‑251 (MQ=255)
                                                                                                                                                                                                                     tgtCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAAAGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCCGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCCCGCGATCTACCGCGAGGt                                                                                                                        >  2:393835/1‑162 (MQ=255)
                                                                                                                                                                                                                     tgtCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAAAGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGt                                                                                                                        <  1:393835/162‑1 (MQ=255)
                                                                                                                                                                                                                         ggcgcgtcgtggtaccc‑GCCAGTTTCGTCAACTGGG‑TGCGGTGGGCGCGCAGCAGGATACTCGTCTCAACGGTACCGTCCCTGGCCTGTTTCAGCGTCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTGCGGTCATCACGCTATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTGTCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTTGGCGGCGTCGATc                            <  2:294873/236‑1 (MQ=255)
                                                                                                                                                                                                                         gAGCGGCTC‑GTTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCg                           <  1:162436/251‑1 (MQ=255)
                                                                                                                                                                                                                           gCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGAAGGCGCGCAGCATGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGcc                         <  1:234483/251‑1 (MQ=255)
                                                                                                                                                                                                                             ggCTC‑GCATACC‑GCCAGCTGCGCCACCGTGA‑TGCTGTAGGCGCGCAGCAGGAAAACCGCTTCAACGTTATCGCCGCGGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCGCTTGCAGGGCGTGGGCGGCGTCGATCGCCtt                       <  2:138887/251‑1 (MQ=255)
                                                                                                                                                                                                                               cTC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTtct                     >  1:338143/1‑251 (MQ=255)
                                                                                                                                                                                                                                tC‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGCGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGACGTGGGCGGCGTCGACCGCCGtctc                    >  2:189678/1‑251 (MQ=255)
                                                                                                                                                                                                                                 ccgttttcc‑gccgGCTTCGCCAATGGGA‑TGGGGTAGGTGCGCCGCAGGCAATTCGCTTCCACGTGAGCGCTGGTGGTCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCTTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCTTCGAGCGCCTtctc                    <  2:520118/244‑1 (MQ=255)
                                                                                                                                                                                                                                 c‑GCTTACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCg                   >  1:151059/1‑251 (MQ=255)
                                                                                                                                                                                                                                 c‑GCTTACC‑GCCAGCTTCGCCAACGGGG‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTTTCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCg                   <  1:328310/251‑1 (MQ=255)
                                                                                                                                                                                                                                     ttACC‑GCCAGCTTCGCCAACGTGA‑TACGGGAGGCGCGCAGCAGGAAAATCGCTTCAACGTTCTCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCATCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCACAGGTCGGGCAACTCGGTATCGCCTCGGCGTCGGCTCTCGTGCAGGGCGTGGGCGGCGTAGAGCGACTTCACGccc                >  2:417650/1‑251 (MQ=255)
                                                                                                                                                                                                                                     tcccc‑GCCAGCTTCGCCAACGTGA‑TGCGGTGGGGGCGCAGCAGGAAAACCGCTTCAACCTTATCGCCGCGGGCCTTTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCGTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGc                  <  2:186009/246‑1 (MQ=255)
                                                                                                                                                                                                                                      tACC‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCGGTTCGCTGTCGGCCATGCCGCCTTCGGTCATCACGCGATCTACCGCGGGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAAACGGGATCGCCTCGGCGTCGGCTATCTTGCAGGGCGAGGGCGGCGTCGATCGCCTTCTCGcccc               >  2:142919/1‑251 (MQ=255)
                                                                                                                                                                                                                                         c‑GCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCtt            >  2:482957/1‑251 (MQ=255)
                                                                                                                                                                                                                                           gCCAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCttt           >  1:80500/1‑251 (MQ=255)
                                                                                                                                                                                                                                             cAGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTc          >  1:67995/1‑250 (MQ=255)
                                                                                                                                                                                                                                             cAGCTTCGCCAAC‑TGGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTGTCAGCGCCTGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCa         <  1:50930/251‑1 (MQ=255)
                                                                                                                                                                                                                                              cgCTTCGCCACCGTGA‑AGCGGTGGGCGCGCGGAGGGAAAACCGCTTCACCCGTAGCGCCGCTGGCCGGCTTCAGCGCCAGCGCCGCCAGGTCGCGGTCGGCAATGCCGCCTTCGGTCATCACTCGATCTACCGCGAGGTTAAACTGCTGGTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCAc        <  2:258990/250‑1 (MQ=255)
                                                                                                                                                                                                                                              aGCTTCGCCAACGTGA‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCACTGCCGCCTTCGGTCATCACGCGATCTACCGCGAGCTTAAACTGCTGTTCAATCTGGGCGCCGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGACGTGGGCGGCGTCGATCACCTTCTCGCCCCCTTTCAc        >  1:109218/1‑251 (MQ=255)
                                                                                                                                                                                                                                                cgtCGCCACCGTGA‑TGGGGTAGGCGCGCAGCAGGAAAATCCCTCTACCGTTATCGCCGCTGGCCTGTTTCCGCGTCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACgg      <  2:365376/249‑1 (MQ=255)
                                                                                                                                                                                                                                                    gCCAACGGGA‑TGGGGTAGGCGCGCAGCAGGAAAAGCGCTTCAACGTTATCGCCGCGGGCCGGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTAACGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTGCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAAc  <  2:47611/251‑1 (MQ=255)
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  
CGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGT‑CAGCGTCGG‑CGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTG‑TAGT‑CGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTC‑GCTTACC‑GCCAGCTTCGCCAACGTGG‑TGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAGCTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAAC  >  NC_000913/4320169‑4320657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑