Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,306,960	G→A	A280A (GCC→GCT)	alsK ←	D‑allose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,306,960	0	G	A	100.0%	203.8 / NA	64	A280A (GCC→GCT)	alsK	D‑allose kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/23);  total (41/23)

ATTTTATTAGGCATTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCT  >  NC_000913/4306723‑4307201                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    aTTTTATTAGGCATTTGTGCTGCAGAGGGAAAATTACAGTAAACCTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCACAAAACGTTGATGCGCTACTATTGATGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATaccgcga                                                                                                                                                                                                                                         >  2:666551/1‑244 (MQ=255)         aGGCATTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTg                                                                                                                                                                                                                                 <  1:48355/250‑1 (MQ=255)               ttGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAGAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGc                                                                                                                                                                                                                          >  1:623150/1‑251 (MQ=255)                        agagGGAAAATTACAGTAAAACTATCATTGCAGAAACTAATATGAGTAGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCAACCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGCTGCGGCAGTGGACgg                                                                                                                                                                                                                 >  2:517175/1‑251 (MQ=255)                         gagGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGgc                                                                                                                                                                                                                >  2:625868/1‑251 (MQ=255)                                    aCAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCTCAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑ttt                                                                                                                                                                                                   <  2:603120/251‑1 (MQ=255)                                          aaaCTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCATTGGCACCGCGGGAAATTGCGCTACTGTGCCAACGATTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGACAATCTTGCTGCACCCTGAGGGCCATTAAAGGCAGAAGATGCGGCAGAACTATAGCGCACGACCTGCTGCGGCCGTGGACGGTG‑CAGGTACATTTTgatc                                                                                                                                                                                              >  2:144062/1‑248 (MQ=255)                                                       agagaataatATGAGTCGTTTTATGTGGTTATTATATATATTGTACCTGGCGATGGCACAGCGGGACATTGCGCTACTGTGACAACGATTTAATCATGGGGCTTTAGCACAGAAATGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCAACCTGAGAGCCATTAAAGTGAGAAGATGAGGAAGCAGTAACGCGCACGACATTATGAGGCAGTGGAAGGCG‑CAGGCAC‑tgatgtgtaatggacacggg                                                                                                                                                                                  >  2:53000/5‑230 (MQ=255)                                                              aataTGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTAGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTcgcg                                                                                                                                                                         >  1:391758/1‑251 (MQ=255)                                                                 atGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTACCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCAAGACCTGATGCGGCAGTGGACGGCA‑CAGGTAC‑TTTTGGGCCATgccaccgcgagatctccgtgg                                                                                                                                                                      >  2:604633/1‑231 (MQ=255)                                                                    aGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGagag                                                                                                                                                                                <  1:183509/238‑1 (MQ=255)                                                                    aGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGagag                                                                                                                                                                                >  2:183509/1‑238 (MQ=255)                                                                        gTTTTATATGTTGCTTATATATATTGTACCTGTCGTTGGCACAGCGGAACATTGCGCTACAGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGCGGCAAAAAACGTTGATCCGCCAATATTGCTGCACCCTGACCGCCCTTAAAGTCAGAGGATGAGGCAGCAATAAAGCGCACGACCTGATGCGTCAGTGGACGGCG‑CAGGGAC‑TTTTGGGTCATGGCACATAGAGTCTCGCGTGGGAAgg                                                                                                                                                                 >  2:30795/1‑249 (MQ=255)                                                                           ttaggtgtTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTACTGGGGCTTTAGCACAGAACTGTGGCAAACAACGTTGATGCGCCAATATTGCTGCACCCTGATCGCCATTAAAATCAGAAGATGACGCAGCAATAAAGCGCACGAACTGATTCGGCAGTGGACAGCG‑CAGGGAC‑TTTTGTGTCATGGAAACGAGAGTCTCGCGTGAGGAGGCGGGc                                                                                                                                                            >  2:484228/5‑251 (MQ=255)                                                                               gtgtTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGATACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGAGCAAGACCTGATGCGGCAGTGGACGGCG‑CAGGGAC‑TTTTGTGGCATGGCAACGAGAGTATCGCGTGGGAAGGCAGGCattt                                                                                                                                                        >  1:385096/1‑249 (MQ=255)                                                                                 gtTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGACAGAAGATGAGGCAGCCATAAAGCGCACGCCCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGACTCGCGTTGTGAGGCGTGCATATcc                                                                                                                                                      >  1:522692/1‑251 (MQ=255)                                                                                   ttattaTATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCa                                                                                                                                                     <  1:74859/250‑1 (MQ=255)                                                                                    tattatATATATTGTACCTGTAGTTGGCACAGCGGGACATTGCGCTACTGTTCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGACAGTGGACGGCGACAG‑TAC‑TTTTGGGTCCTGGCAACGAGAGTCTCGCGTGGGGAGGCGGGCCTATCCAt                                                                                                                                                    >  2:95218/1‑250 (MQ=255)                                                                                              atTGGACCTGTCGTTGGCGCAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCACCGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCg                                                                                                                                          <  2:400715/250‑1 (MQ=255)                                                                                                 gTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGGAC‑TTTTGGGTCATGGCCACGGGAGTCTCGAGGGGGAAGGCGGGCATATCCATCACGCCACCGcccc                                                                                                                                      >  2:183027/1‑250 (MQ=255)                                                                                                   aCCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAgg                                                                                                                                    <  1:625868/251‑1 (MQ=255)                                                                                                         cGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCAcc                                                                                                                              >  1:175691/1‑251 (MQ=255)                                                                                                         cGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCAcc                                                                                                                              >  1:86084/1‑251 (MQ=255)                                                                                                          gTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACgg                                                                                                                                                                                                                 <  1:193978/169‑1 (MQ=255)                                                                                                          gTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGAGGCAGTGGACgg                                                                                                                                                                                                                 >  2:193978/1‑169 (MQ=255)                                                                                                                acaGCGGGACATTGCGCTGCTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGGCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGACACCGCCCAGGATCACCGCATCgg                                                                                                                       >  2:612088/1‑251 (MQ=255)                                                                                                                    cGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATc                                                                                                                   >  1:559526/1‑251 (MQ=255)                                                                                                                       gACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGaa                                                                                                                <  1:194660/251‑1 (MQ=255)                                                                                                                                   cTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGCGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCt                                                                                                    >  1:650205/1‑251 (MQ=255)                                                                                                                                   cTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCt                                                                                                    >  1:538039/1‑251 (MQ=255)                                                                                                                                       gCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCtggt                                                                                                 <  1:671713/250‑1 (MQ=255)                                                                                                                                        ccAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTTGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCtggtgg                                                                                               <  2:421830/251‑1 (MQ=255)                                                                                                                                                  aCTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGGGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGCGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAAAGGCCCg                                                                                     >  1:12616/1‑251 (MQ=255)                                                                                                                                                      aTGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGGGGCAATGGCCCGTGcc                                                                                 >  1:261153/1‑251 (MQ=255)                                                                                                                                                         gggCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCa                                                                                                                                >  1:187062/1‑201 (MQ=255)                                                                                                                                                         gggCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCa                                                                                                                                <  2:187062/201‑1 (MQ=255)                                                                                                                                                         gggCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCcgcg                                                                              <  2:344755/251‑1 (MQ=255)                                                                                                                                                             tttagaacagaaagggggCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCAt                                                                                                                                                    >  1:675919/17‑177 (MQ=255)                                                                                                                                                             tttAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTTAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTTATGCGGCATTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGTGAGTCTCGCGTGGTAAGGCGGGCATATCCAt                                                                                                                                                    <  2:675919/177‑1 (MQ=255)                                                                                                                                                                 gCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCa                                                                        >  1:334132/1‑249 (MQ=255)                                                                                                                                                                  cacaGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGAG‑CAGGAAC‑TTTTGGGTGATGGCAACGCAAGTCTCGCGTGGGAAGGCAGGCATATCCATCACGCCACCGCCCAGGCTCACCGAATCGGGACCGAACAGCTTAATGCTGGGGGAAATGGCCCGTGcacggttttatagc                                                                     >  2:379490/1‑238 (MQ=255)                                                                                                                                                                   acaGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTAATGCGGCAGCGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCACGAGGGAAGGCGGGCGTATCCATCACGCCACCGCCCCGGCTAACCGCATCGGGATCGACCAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTGTTCagac                                                                     >  2:350224/1‑247 (MQ=255)                                                                                                                                                                      gAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGAc                                                                 <  2:228663/251‑1 (MQ=255)                                                                                                                                                                            tgGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCCTTAAAATCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCCGGATCGCAGCCTAGGGATCGAACAGATTAATCATGGCGGCAATGGCCCGTGCCGCGttttacagacaaccctgg                                                            >  2:331353/1‑236 (MQ=255)                                                                                                                                                                                      cGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGc                                                 >  1:264410/1‑251 (MQ=255)                                                                                                                                                                                         tGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGaa                                                                                                                                                                   <  1:587231/134‑1 (MQ=255)                                                                                                                                                                                          gATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGttt                                             >  1:294462/1‑251 (MQ=255)                                                                                                                                                                                           aTGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGCGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGtttt                                            >  1:458413/1‑251 (MQ=255)                                                                                                                                                                                                   atatTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACTGGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGTAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTGTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATgg                                    <  2:538039/251‑1 (MQ=255)                                                                                                                                                                                                    tatTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGCTCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATgg                                    >  1:231926/1‑250 (MQ=255)                                                                                                                                                                                                     atTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGa                                   <  1:612088/250‑1 (MQ=255)                                                                                                                                                                                                        gctgcACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGa                                >  1:540441/1‑250 (MQ=255)                                                                                                                                                                                                              cccTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGaaa                              >  1:384067/1‑246 (MQ=255)                                                                                                                                                                                                              cccTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATc                         <  2:175691/251‑1 (MQ=255)                                                                                                                                                                                                                cTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCCGGATCCCCGCCTCGGGATCGGACAGATTAATGCTGGGGGCAATGGCCCGTGCCCGGTTTTCAAGCCGACTCTGGAAGAAAGAGGCGGTTTCAGCCTGGCCGaaaaaaacgc                       >  2:586932/1‑245 (MQ=255)                                                                                                                                                                                                                 tGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGCGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGAGTTTTCAAGCCGAATCTGGACGAAAGAGGAGTTTTCCGCATGGACGAAAAGATcgcg                      >  1:611747/1‑251 (MQ=255)                                                                                                                                                                                                                  gAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCCAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGCCGAAAAggactcgc                     >  2:505445/1‑244 (MQ=255)                                                                                                                                                                                                                  gAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATCgcgc                     >  1:565467/1‑251 (MQ=255)                                                                                                                                                                                                                        cATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATggg               >  1:450372/1‑251 (MQ=255)                                                                                                                                                                                                                        cATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATggg               <  1:599845/251‑1 (MQ=255)                                                                                                                                                                                                                             aaGTCAGAAGAGGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAAtt          <  1:527521/251‑1 (MQ=255)                                                                                                                                                                                                                                 cAGAAGATGAGGGAGCAATAAAGCGCACGACCGGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTGGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTGCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCg       <  2:334132/250‑1 (MQ=255)                                                                                                                                                                                                                                    aagaTGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGaaa                              <  1:403884/224‑1 (MQ=255)                                                                                                                                                                                                                                     agaTGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCt  <  1:534287/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    ATTTTATTAGGCATTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCG‑CAGGTAC‑TTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCT  >  NC_000913/4306723‑4307201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑