Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,283,043	G→A	F11F (TTC→TTT)	yjcF ←	pentapeptide repeat‑containing protein YjcF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,283,043	0	G	A	100.0%	54.6 / NA	19	F11F (TTC→TTT)	yjcF	pentapeptide repeat‑containing protein YjcF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/9);  total (10/9)

ATGTATTTTCCTGTCCGTTTCTTTCGTGATTTTTCCTGATGGTAAACTATCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCA  >  NC_000913/4282809‑4283274                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                        atggaTTTTCCTGTCCGTTTCTTTCGTGATTTTTCCTGATGGTAAACTATCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCGCTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTTATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTAttt                                                                                                                                                                                                                          <  1:308366/246‑1 (MQ=255)     aTTTTCCTGTCCGTTTCTTTCGTGATTTTTCCTGATGGTAAACTATCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAAcc                                                                                                                                                                                                                     >  1:440558/1‑251 (MQ=255)                                   ccTGATGGTAAACTATCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTa                                                                                                                                                                                       <  2:477572/251‑1 (MQ=255)                                       aTGGTAAACTATCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACaa                                                                                                                                                                                   <  2:534590/251‑1 (MQ=255)                                                               tCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGc                                                                                                                                                            >  2:263917/1‑250 (MQ=255)                                                                     tCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATc                                                                                                                                                      >  1:496136/1‑250 (MQ=255)                                                                         aaTCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCAtttt                                                                                                                                                 <  1:580386/251‑1 (MQ=255)                                                                                                   catcCATTTTGAAAAGAGAGAGGGCTTTCTTGTCAATTAACGTGTTTTTATGATGTTTGCAGTAATTCTCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCATAGCGGAAAAAACATATTATTTAAAACATTATAGCGCAACAAATATCCTTATAATTAACCAGAAATAAAAGACATATTCTCTGCATAATCATTTTCTACGACTGTCAAAACTCGCTCAttt                                                                                                                       >  2:334013/1‑251 (MQ=255)                                                                                                      ccATTTTTAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCAtttttt                                                                                                                    <  1:190957/251‑1 (MQ=255)                                                                                                                 agagagagGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTAt                                                                                                         >  1:140449/1‑251 (MQ=255)                                                                                                                             ttATTGTCTATTATCGTGTTTTTATGATGTTTGCTGTAATTATCTGTTTGTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACTATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTTTCAAAAATCGCTCATTTTTTAATGAGTTTATCTGTTTAatat                                                                                              <  2:182005/250‑1 (MQ=255)                                                                                                                                        tAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAgg                                                                                  >  1:245366/1‑251 (MQ=255)                                                                                                                                                        tgTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGAGATGGACTTGTGACAGGGTGGTTATCGTTAATCAGGCAAAGCGGAAAAAACATATTATTTAAACCCTTATATCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCACTGCATTATCATTTTCTACTACTGTCAAAAATCGCTAATTTTTTCATGAGTTTATTTTTTTCATATTATGGGAAAAGGAGATGTCTTTggg                                                                     >  2:536680/1‑248 (MQ=255)                                                                                                                                                                 tAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTGTCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGt                                                          >  1:501690/1‑250 (MQ=255)                                                                                                                                                                         tGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTCATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTACCCgg                                                 >  2:392596/1‑251 (MQ=255)                                                                                                                                                                                         gTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGACAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGtt                                                                                                            >  1:443736/1‑176 (MQ=255)                                                                                                                                                                                         gTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATAGTATTTAAACCAGTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTTCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGtt                                                                                                            <  2:443736/176‑1 (MQ=255)                                                                                                                                                                                                                  ggTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGcc         <  1:263917/250‑1 (MQ=255)                                                                                                                                                                                                                        cGTTAAACAGGCAAAGAGGAAAAAACATATTATTTAAATCATTATAGCTCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAACATTATGGGAAAAGGTGATGCAGTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCa  <  2:245366/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                        ATGTATTTTCCTGTCCGTTTCTTTCGTGATTTTTCCTGATGGTAAACTATCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCA  >  NC_000913/4282809‑4283274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑