Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,547	C→T	V249V (GTC→GTT)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,547	0	C	T	100.0%	134.9 / NA	48	V249V (GTC→GTT)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/36);  total (12/36)

TTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGC  >  NC_000913/3303‑3780                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          ttGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGa                                                                                                                                                                                                                                      <  1:452553/250‑1 (MQ=255)    atggggtgCTGTGGGTGCGTGCGTACACGGGGATTAAAGGTTCGACGGCAGAAGCAAGGTGTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCAGTGAGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TAGCGCTGAACCCTTCCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATc                                                                                                                                                                                                                                  <  2:536996/244‑1 (MQ=255)    atgaGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAGCCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATc                                                                                                                                                                                                                                  <  1:141789/251‑1 (MQ=255)      gaGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCg                                                                                                                                                                                                                                 <  1:633784/250‑1 (MQ=255)         tGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGGAATCGgcgg                                                                                                                                                                                                                             >  1:17161/1‑250 (MQ=255)                  ggacggaggaaTCCGGGGATTAAAGTGTCGACGGCTTAAGCCAGGTCTCTGTGCCCGGCTAATTATCGCCGCCAGGATTGCATTGCGCACGTGCGACATCGGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTCAGCTTGCCGCGAAGCTGATGAAAGCTTT‑TATCGCGGAACCCTACCGTGAACGGTTCCTGCCTGGCTTCCGGCAGGCGCTGCAGGCGTTTGCGAAAATCGGCGAGGTagcgag                                                                                                                                                                                                                    <  2:28355/241‑1 (MQ=255)                      tGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGAATGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGACGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTGCTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGTGGaaaatgacgcggggagagaaggg                                                                                                                                                                                                                >  2:387441/1‑231 (MQ=255)                          gTATCCGGGGATTAAAGTCCCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTAtct                                                                                                                                                                                                            <  2:17161/251‑1 (MQ=255)                            aTCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGGAGCGGGCGGTATctcc                                                                                                                                                                                                          >  1:400180/1‑251 (MQ=255)                                ggggATTAAAGTCTCGACGGCAGAAGTCAGGGCTATTTTACCGGGGCAGTATCGCCGCCAGGATGGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTAGTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGGAGCGAGCGGTATctccggct                                                                                                                                                                                                      <  2:209199/251‑1 (MQ=255)                                  ggatgaacggttcGACGGCAGAAGCCAGGGCTATTTTTCCGGCGCAGTAGCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATctccggctcc                                                                                                                                                                                                    <  2:400180/240‑1 (MQ=255)                                  ggATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCCCGGTAGCGAGCGGGATctccggctcc                                                                                                                                                                                                    >  1:259656/1‑251 (MQ=255)                                              cGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGGAATCGGCGCGGGAGCGAGCGGTGTCTCCGGCTCCGGCCCGAccctt                                                                                                                                                                                        >  2:143563/1‑248 (MQ=255)                                                   gCAGAAGCCCGGGCTATTTGACCGGCGCTGGATCGCCGCCAGGAGTGCTTTGCGCATGGGCGACATCTGGCTGGCTTCATTCACGCCGGCTATGCCCGTCAGCCTGAGCTTGCTGCGAAGTTGATGAAAGATGT‑GAGCGCTGAACCCGACCGTGAACGGTTACTGCCTGGCTTCCGGCATGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCGCGACCGTGTTCGc                                                                                                                                                                                   <  2:611675/251‑1 (MQ=255)                                                        aGCCAGGGCTATTTTCCCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACAGCTGGCTGGCTTCATTCTCGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCtgt                                                                                                                                                                              <  2:580161/251‑1 (MQ=255)                                                                            cgcAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGcc                                                                                                                                                          <  1:612735/251‑1 (MQ=255)                                                                                   ccgccgcCAGGATTTCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGcgcg                                                                                                                                                   <  2:239528/250‑1 (MQ=255)                                                                                          cAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGa                                                                                                                                            <  1:199385/251‑1 (MQ=255)                                                                                          cAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATAGGCGCGGTATCGAGCGGTATCTCCGGCTCCGGCCCGACCTTTTTCGCTCTTTGAGACAAACCGGATACCGCCCAGCGCGTTGCCgc                                                                                                                                            >  1:24179/1‑250 (MQ=255)                                                                                                   attgcGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGGTACCGCCCAGCGCGTTGCCGACTGGTTggg                                                                                                                                   >  1:222923/1‑251 (MQ=255)                                                                                                    ttgcGCACGGGCGACAGTTGGATGGCTTCAGTCAAGCCTGCTATTCCCGACAGCCTGAGTTTGCCGCGAAGCTGATGAAAGATGG‑TATCGCTGCACCCTGCCGTGAACGGTTACTGCCTGGCTGCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTggg                                                                                                                                   <  2:65022/250‑1 (MQ=255)                                                                                                      gcgcCCGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTACTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACATTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTaa                                                                                                                                <  1:502385/251‑1 (MQ=255)                                                                                                          aCGGGCGAGATCTGGTTGGGTTCATTCACGCCTGCTATCCCCGTCAGCCTGTGCTGTCCGCGAAGCTGATGAAAGATGT‑TAACGCGGAACCCGAGCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGGAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTTACAAGCCGGATACCGCCCAGCGCGTTGCCTACTGGTTGGGTAAGAAc                                                                                                                            <  2:292297/251‑1 (MQ=255)                                                                                                                       ggctggCTTCATTCACGCCTGCTATTCCCGTTAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATc                                                                                                               <  1:236460/247‑1 (MQ=255)                                                                                                                                       gCCTGCTATTCCCGTCAGCCTGAGCTGGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCa                                                                                               <  1:139006/251‑1 (MQ=255)                                                                                                                                          tGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGGAATCGGCGCGGTAGCGAGCGGTATCTCCCGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTTGGTAAGAACTACCTGCAAAATCAGGAAGGGTTTGTTCatat                                                                                            >  1:365009/1‑251 (MQ=255)                                                                                                                                             ttttCCCGGCAGCCTTGGCTTGCCGCGAATCTGATGAAAGA‑GTGTATCGCTGAACCCTTCCGTGAACGGTTACTGCCTTGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGTAATCGGCGCTGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTGCCTGCAAAATCAGGAAGGTTTTGTTCATAttt                                                                                          <  2:409996/248‑1 (MQ=255)                                                                                                                                                 cccGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCgg                                                                                     <  2:416242/251‑1 (MQ=255)                                                                                                                                                  ccGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGc                                                                                    <  1:568262/251‑1 (MQ=255)                                                                                                                                                            gAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTACGGCTCCGGCCCGCCCTTGTTCGCTCTGTGTGGAAAGCAGGATACCTCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTAACTGCCAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATAcggc                                                                          >  1:45209/1‑251 (MQ=255)                                                                                                                                                                 tGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACggcgggc                                                                      <  1:218711/250‑1 (MQ=255)                                                                                                                                                                 tGCCGCGAAGCTGATGAAAGATGG‑TGGCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCTGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCGCCGGCTCCGGCCCGACCTTGTTCGCTCTGGGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACggcgggc                                                                      <  2:573873/250‑1 (MQ=255)                                                                                                                                                                       gAAGCTGATGAAAGATGT‑TATCGCTTAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAg                                                               <  1:439399/251‑1 (MQ=255)                                                                                                                                                                       gAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAACCAGCGGGAACTCCGGCTCCGGCCCGGCCTTGTTCGCTCTGTTGGACAAACCGGGTACCGCCCCGCGCGTTTGCGACCGGTTGGGTAAGAAATACCCGCCAAATCAGGGAGGGTTTGGTCATATTTGCCgggcgggtacgggggggggaagg                                                                >  2:637205/1‑229 (MQ=255)                                                                                                                                                                        aaGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCGCGAGt                                                              <  1:93018/251‑1 (MQ=255)                                                                                                                                                                         aGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGGAATTGGCGCGGTAGCGAGAGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACCAGACGGAAACCGCCCCGCGCGGTGCCGCCTGGATGGGGAAGAACATCCTGCCAACACAGGAAGGGTTTGTTCATATTTTCCGGCTGGGAACggcggggagagcg                                                                >  2:315958/1‑241 (MQ=255)                                                                                                                                                                             gatgaAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACt                                                           <  1:303490/249‑1 (MQ=255)                                                                                                                                                                                gaAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGaaa                                                      <  1:330807/251‑1 (MQ=255)                                                                                                                                                                                   aGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACt                                                   <  1:186893/251‑1 (MQ=255)                                                                                                                                                                                        t‑tATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAACCAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGGCTGGGTGGGGAAGAACTACCTGCAAAAACAGGAAGGGTTTTTTCATATTTGCCGGCTGGATACGGGGGGGGCAcagggacatgaaaaatgaaa                                               >  1:300655/1‑231 (MQ=255)                                                                                                                                                                                                    cccTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACaa                                   <  1:192179/251‑1 (MQ=255)                                                                                                                                                                                                     ccgaCCGTGTAAGGTTACTGCCTGGCGTCCGGCAGGCGCGGCCGTCGGTTGCGTAAATCGGCGCGGTAGCTAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTTGTTGTGTAAGACCTACCTGCAAAATCAGGAAGGTTTTGATCATATTTGCCGGCTGGATACGGCGGGCTCTCGAGTACTGGAAAACTAAATGAAACTCTACAAt                                  <  2:345240/248‑1 (MQ=255)                                                                                                                                                                                                     ccTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAAt                                  <  1:248952/251‑1 (MQ=255)                                                                                                                                                                                                        aCCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACTGCGGGCGCACGAGTACTGGAAAACTAAATGAAACGCTACAATCt                                <  1:553685/250‑1 (MQ=255)                                                                                                                                                                                                                      cggcctggCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACaatga                 <  2:222923/245‑4 (MQ=255)                                                                                                                                                                                                                        gcctggCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACaatga                 <  1:143563/245‑4 (MQ=255)                                                                                                                                                                                                                         cctggcatCCGGCTGGCGCGGCAGGCGGTTGCGTAAAGCTGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTGGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTTAGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGAGACGGCGGGCGCACGAGTACTGGAAAACGAAATGAAACTCGACAATCTGAAAGATcacgttgagca              <  2:278036/244‑9 (MQ=255)                                                                                                                                                                                                                                     caggcgcggcaggcgGTTGCGTAAATCGGCTCGTTAGCGGGCGGTATCTCCTGCTCCGGCCCGACCTTGTTCGCGCTGTGTTACAAGCCGGATACCGCCCAGCGCGTTGCCGTCTGGTTGGGTAAGATCTACCTGCAAAATCAGGAAGGTTTTGTGCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAACGAATCTTTCCAATCTGAAAGATCGCAATGAGCAgggaatctgtgc  <  2:281530/251‑11 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          TTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGC  >  NC_000913/3303‑3780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑