| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,193,996 | T→G | D70A (GAT→GCT) | thiE ← | thiamine phosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,193,996 | 0 | T | G | 94.2% | 268.9 / 0.6 | 87 | D70A (GAT→GCT) | thiE | thiamine phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base T (2/3); new base G (43/39); total (45/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
ACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTC > NC_000913/4193747‑4194241 | aCATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCTCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATc < 1:341424/251‑1 (MQ=255) aTGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGt < 1:374304/251‑1 (MQ=255) gCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCCGTAAGCGTTg > 1:468130/1‑251 (MQ=255) cGTGCCAGCTGTTCCAGCCCCTGCGGAGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCATCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGAt > 1:509006/1‑251 (MQ=255) gCCAGCTGTTCCAGCCCTTGCGGTGCAGAAGGCTGCTGTTTGGTTTGCGTCGGGAACACATGGCCCAGCGCTATATAAGAGGGGCGTGCTTCAAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCTAGCCCCAGGCCTGCCGCGCGGAGGGCATGGAGTTCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGCCGCCAGTGGCCTGATGCTTGAGCGCCAGCCGCCAGTAAGCGTTGATaaa < 1:257798/251‑1 (MQ=255) ccAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATcgcgcg > 1:260754/1‑251 (MQ=255) gCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCTATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTta < 1:30074/251‑1 (MQ=255) tGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGCTTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCACCAAATGGACGCCATACGCCTGATGCTTTATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAgcg > 2:374755/1‑251 (MQ=255) gTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGcggcgg < 1:292065/251‑1 (MQ=255) gaaagCATCTGTTTGGTTTGCGTAGGGAACACAGGTCCCAGCGCGATATAAAAGAGGCGTGCTACCAGCGCGACGTCGCTTTCCATATCGTCATGTGTCGAAACGCCCAACCGCAGGCCTGCCGCAAGGAGGGCAGTGAGATCGGTGGCTTTAAAATCTTACTACCCCAGAAGGACACCATACGCCTGATACTGGAACGCCAGCCACCAGTAAGCGTTGATAAAAAATCGCGCGATAAAGcggcggacc > 2:534326/5‑246 (MQ=255) gtttggtttgCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTgcc > 1:390840/1‑251 (MQ=255) tttggtttgCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTgccg > 2:240156/1‑251 (MQ=255) tttggtttgCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGGCGATTTCCATATCGTCATGTGTCGCAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGAACGCCATACGCCTGATGCTTGATCGACAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAAGCGAAATTgcg > 2:607041/1‑250 (MQ=255) ggAACACAGGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCAGATCGTCATGTGTAGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCt < 1:202993/251‑1 (MQ=255) aaCACATGTCCCAGCGCGATCTAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATAGTCATGCATCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAGTGGACGCCATACGCCTGATGCTTTATCGCCAGCCGCCAGTCAGCGTTGATAAACAATCGCGCGTTATAACGGCGGCCTA‑GCCCAATTgccagcacgccctacgatcc > 2:315145/1‑234 (MQ=255) cacaTGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTccc > 1:148468/1‑250 (MQ=255) aGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATc > 1:319101/1‑251 (MQ=255) ataAGAGGGGCGTGCGGCCAGCGCGACGGCGATTTCCATTGCGTAATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTCCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCAACCTCTTCATCGCGCCGAt < 1:632932/251‑1 (MQ=255) aaGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATc > 2:546379/1‑180 (MQ=255) aaGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGAGCGCCAGCCGCCAGTAAGCGTTGATAAACAATc < 1:546379/180‑1 (MQ=255) gagGGGCGTGCTGCCAGCGGGACGTCTATTTCCATATCGTTATGTGTCGAAACGCCTAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCttt < 1:606014/251‑1 (MQ=255) gagGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCttt < 1:261062/251‑1 (MQ=255) agGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCtttt > 1:170306/1‑250 (MQ=255) ggTGCTGCAAGCGCGCCTTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCg < 1:234559/250‑1 (MQ=255) cGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCg > 1:9867/1‑251 (MQ=255) ctgcCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCACTTGCCGCCACGACCTCGGCTTCCCCCCCTTCATCGCGCCGATCtttttttcgtgac > 2:589266/1‑241 (MQ=255) ggcCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCt < 1:661552/250‑1 (MQ=255) gcCAGCCCGCCGTCGATTTCCATATCGTCTTGTGTCGAAACGCCCCGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGTTCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTg < 1:514685/251‑1 (MQ=255) cAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTc < 1:374772/213‑1 (MQ=255) cgACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAgta < 1:131267/251‑1 (MQ=255) gACGTTGATTCCCATATCGTCATGTTTCAAACCCCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGTTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATCCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACAGCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAgtac < 1:447498/251‑1 (MQ=255) acttCGATTTCCATATCGTCTTGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAgtacg < 1:67223/248‑1 (MQ=255) cGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAgtacgt < 1:240156/251‑1 (MQ=255) gTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTGAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCC‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCtt > 1:671385/1‑227 (MQ=255) tCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGCGAgtacgtac > 1:139507/1‑251 (MQ=255) gATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGc > 1:458524/1‑251 (MQ=255) tttCCATATAGTAATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAACTCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGAGTTGATAAACAATCGCGCGTTATCGCGGCGGCCCA‑GCGCAATTTCCGCCACGACATCGGCTTCCCACTCGTCTCCGCGCCGATATTTGATGAGCAGCAGGAGAggacgtcagccc > 2:661290/1‑245 (MQ=255) ccATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGc > 1:655659/1‑250 (MQ=255) ttgttgAAACCCCCAGCCGAAGGCCTGCCGCGCGGATGGCATGGGGATCGGTGGCTTGATAATCTTCCTGCCCCAAAGGGCCGCCTTACGCCTGATGCTTGATCGCCAGTCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACg < 1:661875/245‑1 (MQ=255) tgtCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGtt > 1:408072/1‑251 (MQ=255) cGAACCGCCCCGCCTCAGGCCTGCCGCGCGGATGGCATGGAGACCGGTGGCTTGCAATTCTTCCGGCCCAAAATGGACGCCATACGCTTGATGCTTGCTCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGa < 1:420054/251‑1 (MQ=255) gAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGAt > 1:349332/1‑251 (MQ=255) aCGCCCAGCCGCGGCCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATCCGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCa < 1:48810/251‑1 (MQ=255) cAGCCGAAGGCCTGCCGCGCGGATGGCATGGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATCGCGGCGGCCCA‑GCGCAATTGCCGCCACGCCATCGGCTTCAACCTCTTCATCGCGCCGATCTTTGATGCGTAGCAGGAGAGTAGGTACGCCTACATCCAACAGACGTTCGTACCACTGt > 2:604159/1‑250 (MQ=255) cGCAGGCCTTCCGCGCGGAGGGCAGTGAGATCGGTGGTGTGAAAACCTTCCTCCCCCAATGGGACGCCTTCCGCCTTATGCTTTATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCTTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCt < 2:260754/251‑1 (MQ=255) cAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGt > 1:15254/1‑251 (MQ=255) cAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCCATCTTTGGTGCGTAGCTGGAGAGAACGTACGCCCGCATCCTACAGACGTTCGATCCACTGtctcctgg > 2:454099/1‑244 (MQ=255) gccgcccGCGCGGATGGCATTGAGATCTGTGGCTTGCAAATCTGCCTGCCCCAAAGGGACGCCATACGCCGGATGCTGGATCGCCAGCCGCCATTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTcca < 1:403480/246‑1 (MQ=255) gCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGCTAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATAGGCTTCCCCCTCTTAATCCCGCCGATCTTTGATGCGGAGCTGGAGAGTACGTAAGCCTGCATCCCAAAGAACTTCCATCCACTTTACGCTGTcaacccc > 2:375200/1‑249 (MQ=255) cgGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGAGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGCTCTTTGATGCGTAGCTGGAGTGGACGTACGCCGGCATCCAACAGACGATCGATCCACTGTACGCTGCCCCCCCCCggga > 2:486096/1‑249 (MQ=255) ggATGGCCTTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTTCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACACCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCCCCACCGGGTAc > 2:671209/1‑251 (MQ=255) cGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGaaa < 1:374755/250‑1 (MQ=255) ggTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCCCGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGga > 2:180399/1‑173 (MQ=255) ggTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGga < 1:180399/173‑1 (MQ=255) gCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTAc > 1:385777/1‑251 (MQ=255) cTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACCTCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCCCCGGGTACAGTCCTGAACGAAAAGGTAc > 2:193141/1‑250 (MQ=255) ttGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGGACGTACGCCTGCATCCAACAGACGTTCGCTCCCCTGTACGCTGTCCACCACCGGGGACAGTCCGGGACGAAAAgggtc > 2:236108/1‑246 (MQ=255) tGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACag < 1:453018/250‑1 (MQ=255) gCAAATCTTCCTGCCCCAAAGGGACGCCATACGCCTGTTGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACagga < 1:167657/251‑1 (MQ=255) cAAATCTTCCTGCCCCAACTGGACGAACTACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGATGATAAACAATCGCGCGTTAAAGCGGCGGCCCA‑ACGCAATTGCCGCCACGACATAGGCTTCCACCCCTTCATCGCGCCGATCTTTGATGCGTAGATGGAGAATACGTACGCCTGCATCCAACAGGCGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGaccgcaaacgaacagg > 2:631818/1‑234 (MQ=255) aaTCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACaggagg > 1:219739/1‑250 (MQ=255) tccgcccccAAATGGACGCCTTACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAAt < 1:104033/245‑1 (MQ=255) ccggCCCCAAACGGACCTCATACGGCGGATGCTTGTCCGCCGGCCGCCAGTAAGCTGTGATAAACAATCGCGCTTTATAGCGGCGGCCCA‑GCCCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGGACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCa < 2:342550/248‑1 (MQ=255) cTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATc > 1:257253/1‑249 (MQ=255) cTGCCCCAAATGGACGCCATACGACGGATGCTTGATCGCAAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATAGGCTTCCAACTCTACATCGCGCCGCTCTTTGCTGCGCAGCTGGAGCGTACGTCCGCCTGCATCCAACAGACGGTCGATCCACTGCACGCTGTACCCCCCCGGGTACAGTCCTGCAAGACAAGGGCCAGGAGGGAAATc > 2:42918/1‑249 (MQ=255) gCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAg < 1:223950/249‑1 (MQ=255) cAAATGGCCGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGAt < 1:16391/251‑1 (MQ=255) cAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGCTAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGCGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGCAAAGGTACAGGAGGAAAATCACGCTGa > 1:646907/1‑250 (MQ=255) aaTGGACGCCATACGCCTGATGCTGGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCCTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATAc < 1:236108/251‑1 (MQ=255) ggACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACAt < 1:302902/250‑1 (MQ=255) aCGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCAACGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCa > 1:410792/1‑250 (MQ=255) ccgccggattcttgacgcccacccgccAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTtcct < 1:370634/229‑1 (MQ=255) acgctggATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAACCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTtcct < 1:607041/245‑1 (MQ=255) accctggttGCTTGACCGCCAGCCCCCAGTAAGCGTTGATAAACAACCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCGTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTGCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTtcct < 2:139507/243‑1 (MQ=255) gCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTtcctcc > 1:429736/1‑251 (MQ=255) gtagtcttgacccccAGCCGCCGGTACGCGTTGATAACCAATCTCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGAACTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTa < 2:349332/238‑1 (MQ=255) aTGCTTGATCGCCAGCCGCCAGGAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑ACGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGAACTTTGATGCGTAGCTGGCGAGTACGTACGCCTGCATCCAACAGACGTTCGATACACTGGACGCTGTCCACCACCGGAGACAGTCCTCAACGACAAGGGACAGGCGGAACATACGGCTGATACATCTCGCTTCCCCCTTAcc > 2:193871/1‑250 (MQ=255) tGCTTGATCGCAAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCCCAATTGCCGCCACGACATCGGCTTCCACCTCCTCATCGCGCCGCTCCTTGCAGCGTAGCTGGAGAGTACGTACGCCCGCATCCAACTGACGTTCGATCCACTGAACGCCGTCACCCACCGGGGACACTCCTGCACGGAAACATACAGGAGGGCAAACAAGCTGATACAGCACGCATCCCCCTTCCGc > 2:17030/1‑251 (MQ=255) tGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGt > 1:643434/1‑251 (MQ=255) gATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACACCGGCTTCCACCTCTTCATCGCGCCGAGCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTa < 1:584912/251‑1 (MQ=255) atgacaggctgacAGTAAGCGTTGAAAAATAATCGCGCGTTATAGCGGCGGCCAA‑GCGCAATTGCCGCCCCGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTCGATGAGTAGCTGGAGAGTACCTGCGCCTGCATCCAACAGACGTTCTATCCACGGTACGCTGTCCACCACCGGGTACAGTCCTGATCGAAAAGGTACAGGAGGAAAATCATGATGATACATCACGCTTCCTCCTTACGCAGGTAg < 2:438908/239‑1 (MQ=255) cAGCCGCCAGTAATCTTTTATAAACAATCGCGCGTTATAGCGGCGGCCCT‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACTCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTtc < 2:577865/251‑1 (MQ=255) cAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑TCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTtc < 1:387082/251‑1 (MQ=255) gccAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGc > 1:405076/1‑251 (MQ=255) ccAGTAAGCGTTGATAACCACTCGAGCGTTATCGCGGCGGCCCA‑ACGCACTTGACGCCACGACCTCGGCTTACACCTCTTCATCGCGCCGAACTTTGCTGCGTAACTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCCCTGTACGCTGTCCACCCCCCGATAAAGTCCTGAACGAAAAGGTAAAGGCGTACAATCAGGCCAATACATCACGCTTCCTCCTTCCGCCGGGAGATTTCTCCGcc > 2:394280/1‑251 (MQ=255) cAGTAATCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCATCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCct < 1:193141/251‑1 (MQ=255) aGTAAGCGTTGATAAACAATCGAGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCAAACAGACGTTCGATCCACTGTACGCTGTCCCACACCGGGTACAGTCATGCACGAAAAGGGACAGGGGGAAAATCAGGCTGATACATCAAGCTTCCTCCTTACGCAGGTAGATTTCTCCGCctc > 1:220628/1‑251 (MQ=255) | ACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCA‑GCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTC > NC_000913/4193747‑4194241 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |