Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,520	A→T	P240P (CCA→CCT)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,520	0	A	T	100.0%	163.7 / NA	54	P240P (CCA→CCT)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (19/35);  total (19/35)

GACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCT  >  NC_000913/3275‑3750                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       gACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCCCGCCTGCTCTTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTCCTGCCTGGCtt                                                                                                                                                                                                                                   >  2:31102/1‑251 (MQ=255) gACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCtt                                                                                                                                                                                                                                   <  1:547494/251‑1 (MQ=255)  aCATCATCAGCCAGCAAGCGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTc                                                                                                                                                                                                                                  >  1:190210/1‑251 (MQ=255)   catcatCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTCCTGCCTGGCTTcc                                                                                                                                                                                                                                 >  1:608267/1‑251 (MQ=255)              agcAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCcggcaggcgcgg                                                                                                                                                                                                                      <  1:382460/251‑1 (MQ=255)                       cAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCggct                                                                                                                                                                                                             >  1:278036/1‑249 (MQ=255)                             ttGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGa                                                                                                                                                                                                        <  1:452553/250‑1 (MQ=255)                                atggggtgCTGTGGGTGCGTGCGTACACGGGGATTAAAGGTTCGACGGCAGAAGCAAGGTGTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCAGTGAGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TAGCGCTGAACCCTTCCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATc                                                                                                                                                                                                    <  2:536996/244‑1 (MQ=255)                                atgaGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAGCCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATc                                                                                                                                                                                                    <  1:141789/251‑1 (MQ=255)                                  gaGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCg                                                                                                                                                                                                   <  1:633784/250‑1 (MQ=255)                                     tGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGGAATCGgcgg                                                                                                                                                                                               >  1:17161/1‑250 (MQ=255)                                     tGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGGCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCcggcggctgaggacaacggcgc                                                                                                                                                                                               >  1:352715/1‑235 (MQ=255)                                             ggTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGtttgggcaatcgggacgggcgacc                                                                                                                                                                                       >  2:612735/1‑228 (MQ=255)                                              ggacggaggaaTCCGGGGATTAAAGTGTCGACGGCTTAAGCCAGGTCTCTGTGCCCGGCTAATTATCGCCGCCAGGATTGCATTGCGCACGTGCGACATCGGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTCAGCTTGCCGCGAAGCTGATGAAAGCTTT‑TATCGCGGAACCCTACCGTGAACGGTTCCTGCCTGGCTTCCGGCAGGCGCTGCAGGCGTTTGCGAAAATCGGCGAGGTagcgag                                                                                                                                                                                      <  2:28355/241‑1 (MQ=255)                                                  tGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGAATGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGACGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTGCTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGTGGaaaatgacgcggggagagaaggg                                                                                                                                                                                  >  2:387441/1‑231 (MQ=255)                                                      gTATCCGGGGATTAAAGTCCCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTAtct                                                                                                                                                                              <  2:17161/251‑1 (MQ=255)                                                        aTCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGGAGCGGGCGGTATctcc                                                                                                                                                                            >  1:400180/1‑251 (MQ=255)                                                            ggggATTAAAGTCTCGACGGCAGAAGTCAGGGCTATTTTACCGGGGCAGTATCGCCGCCAGGATGGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTAGTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGGAGCGAGCGGTATctccggct                                                                                                                                                                        <  2:209199/251‑1 (MQ=255)                                                              ggatgaacggttcGACGGCAGAAGCCAGGGCTATTTTTCCGGCGCAGTAGCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATctccggctcc                                                                                                                                                                      <  2:400180/240‑1 (MQ=255)                                                              ggATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCCCGGTAGCGAGCGGGATctccggctcc                                                                                                                                                                      >  1:259656/1‑251 (MQ=255)                                                                          cGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGGAATCGGCGCGGGAGCGAGCGGTGTCTCCGGCTCCGGCCCGAccctt                                                                                                                                                          >  2:143563/1‑248 (MQ=255)                                                                               gCAGAAGCCCGGGCTATTTGACCGGCGCTGGATCGCCGCCAGGAGTGCTTTGCGCATGGGCGACATCTGGCTGGCTTCATTCACGCCGGCTATGCCCGTCAGCCTGAGCTTGCTGCGAAGTTGATGAAAGATGT‑GAGCGCTGAACCCGACCGTGAACGGTTACTGCCTGGCTTCCGGCATGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCGCGACCGTGTTCGc                                                                                                                                                     <  2:611675/251‑1 (MQ=255)                                                                                    aGCCAGGGCTATTTTCCCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACAGCTGGCTGGCTTCATTCTCGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCtgt                                                                                                                                                <  2:580161/251‑1 (MQ=255)                                                                                                        cgcAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGcc                                                                                                                            <  1:612735/251‑1 (MQ=255)                                                                                                               ccgccgcCAGGATTTCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGcgcg                                                                                                                     <  2:239528/250‑1 (MQ=255)                                                                                                                      cAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGa                                                                                                              <  1:199385/251‑1 (MQ=255)                                                                                                                      cAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATAGGCGCGGTATCGAGCGGTATCTCCGGCTCCGGCCCGACCTTTTTCGCTCTTTGAGACAAACCGGATACCGCCCAGCGCGTTGCCgc                                                                                                              >  1:24179/1‑250 (MQ=255)                                                                                                                            tgcattgcGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCcgg                                                                                                                                                                                                                               <  2:488172/132‑1 (MQ=255)                                                                                                                            tgcattgcGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCcgg                                                                                                                                                                                                                               >  1:488172/1‑132 (MQ=255)                                                                                                                               attgcGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGGTACCGCCCAGCGCGTTGCCGACTGGTTggg                                                                                                     >  1:222923/1‑251 (MQ=255)                                                                                                                                ttgcGCACGGGCGACAGTTGGATGGCTTCAGTCAAGCCTGCTATTCCCGACAGCCTGAGTTTGCCGCGAAGCTGATGAAAGATGG‑TATCGCTGCACCCTGCCGTGAACGGTTACTGCCTGGCTGCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTggg                                                                                                     <  2:65022/250‑1 (MQ=255)                                                                                                                                  gcgcCCGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTACTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACATTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTaa                                                                                                  <  1:502385/251‑1 (MQ=255)                                                                                                                                      aCGGGCGAGATCTGGTTGGGTTCATTCACGCCTGCTATCCCCGTCAGCCTGTGCTGTCCGCGAAGCTGATGAAAGATGT‑TAACGCGGAACCCGAGCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGGAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTTACAAGCCGGATACCGCCCAGCGCGTTGCCTACTGGTTGGGTAAGAAc                                                                                              <  2:292297/251‑1 (MQ=255)                                                                                                                                                   ggctggCTTCATTCACGCCTGCTATTCCCGTTAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATc                                                                                 <  1:236460/247‑1 (MQ=255)                                                                                                                                                                   gCCTGCTATTCCCGTCAGCCTGAGCTGGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCa                                                                 <  1:139006/251‑1 (MQ=255)                                                                                                                                                                      tGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGGAATCGGCGCGGTAGCGAGCGGTATCTCCCGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTTGGTAAGAACTACCTGCAAAATCAGGAAGGGTTTGTTCatat                                                              >  1:365009/1‑251 (MQ=255)                                                                                                                                                                         ttttCCCGGCAGCCTTGGCTTGCCGCGAATCTGATGAAAGA‑GTGTATCGCTGAACCCTTCCGTGAACGGTTACTGCCTTGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGTAATCGGCGCTGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTGCCTGCAAAATCAGGAAGGTTTTGTTCATAttt                                                            <  2:409996/248‑1 (MQ=255)                                                                                                                                                                             cccGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCgg                                                       <  2:416242/251‑1 (MQ=255)                                                                                                                                                                              ccGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGc                                                      <  1:568262/251‑1 (MQ=255)                                                                                                                                                                                        gAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTACGGCTCCGGCCCGCCCTTGTTCGCTCTGTGTGGAAAGCAGGATACCTCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTAACTGCCAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATAcggc                                            >  1:45209/1‑251 (MQ=255)                                                                                                                                                                                             tGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACggcgggc                                        <  1:218711/250‑1 (MQ=255)                                                                                                                                                                                             tGCCGCGAAGCTGATGAAAGATGG‑TGGCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCTGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCGCCGGCTCCGGCCCGACCTTGTTCGCTCTGGGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACggcgggc                                        <  2:573873/250‑1 (MQ=255)                                                                                                                                                                                                   gAAGCTGATGAAAGATGT‑TATCGCTTAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAg                                 <  1:439399/251‑1 (MQ=255)                                                                                                                                                                                                   gAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAACCAGCGGGAACTCCGGCTCCGGCCCGGCCTTGTTCGCTCTGTTGGACAAACCGGGTACCGCCCCGCGCGTTTGCGACCGGTTGGGTAAGAAATACCCGCCAAATCAGGGAGGGTTTGGTCATATTTGCCgggcgggtacgggggggggaagg                                  >  2:637205/1‑229 (MQ=255)                                                                                                                                                                                                    aaGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCGCGAGt                                <  1:93018/251‑1 (MQ=255)                                                                                                                                                                                                     aGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGGAATTGGCGCGGTAGCGAGAGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACCAGACGGAAACCGCCCCGCGCGGTGCCGCCTGGATGGGGAAGAACATCCTGCCAACACAGGAAGGGTTTGTTCATATTTTCCGGCTGGGAACggcggggagagcg                                  >  2:315958/1‑241 (MQ=255)                                                                                                                                                                                                         gatgaAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACt                             <  1:303490/249‑1 (MQ=255)                                                                                                                                                                                                            gaAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGaaa                        <  1:330807/251‑1 (MQ=255)                                                                                                                                                                                                               aGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACt                     <  1:186893/251‑1 (MQ=255)                                                                                                                                                                                                                    t‑tATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAACCAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGGCTGGGTGGGGAAGAACTACCTGCAAAAACAGGAAGGGTTTTTTCATATTTGCCGGCTGGATACGGGGGGGGCAcagggacatgaaaaatgaaa                 >  1:300655/1‑231 (MQ=255)                                                                                                                                                                                                                                cccTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACaa     <  1:192179/251‑1 (MQ=255)                                                                                                                                                                                                                                 ccgaCCGTGTAAGGTTACTGCCTGGCGTCCGGCAGGCGCGGCCGTCGGTTGCGTAAATCGGCGCGGTAGCTAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTTGTTGTGTAAGACCTACCTGCAAAATCAGGAAGGTTTTGATCATATTTGCCGGCTGGATACGGCGGGCTCTCGAGTACTGGAAAACTAAATGAAACTCTACAAt    <  2:345240/248‑1 (MQ=255)                                                                                                                                                                                                                                 ccTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAAt    <  1:248952/251‑1 (MQ=255)                                                                                                                                                                                                                                    aCCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACTGCGGGCGCACGAGTACTGGAAAACTAAATGAAACGCTACAATCt  <  1:553685/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       GACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGT‑TATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCT  >  NC_000913/3275‑3750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑