Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,424	A→T	A208A (GCA→GCT)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,424	0	A	T	100.0%	178.7 / NA	57	A208A (GCA→GCT)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (28/29);  total (28/29)

ATGGGCGAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAAC  >  NC_000913/3179‑3658                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           aTGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCtt                                                                                                                                                                                                                                       <  1:259432/251‑1 (MQ=255)                          ccGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGGTTGCATTGCGCCCGGGCGACA‑TCTGGCTGGCTTCATTCCAGCCTGCTATTCCCGTCac                                                                                                                                                                                                              >  2:218711/1‑250 (MQ=255)                          ccGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTc                                                                                                                                                                                                                >  2:141789/1‑249 (MQ=255)                             gcagcaTGCATTCAGACAACTTGGCACCGTGTTTTCTTGGTGGTATGCGGTGTATGATCGAAGAAAACGACATCAGCATCCAGCAAGTGCCAGGTTTTGGTGAGTGGCTGTGTGTGCTGTCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGTCTATTTTACCTGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGGTTCATTCACGCCTGCTATTCCCGTCAGCCt                                                                                                                                                                                                           <  2:183510/251‑1 (MQ=255)                                 cattcattACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGc                                                                                                                                                                                                       >  1:582811/1‑251 (MQ=255)                                 cattcattACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGc                                                                                                                                                                                                       >  1:416242/1‑251 (MQ=255)                                    tcattACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCtt                                                                                                                                                                                                     <  1:496142/250‑1 (MQ=255)                                    tcattACGACAACGTGGCACCGTGTTTTCTTGGGGGTATGCAGTTGATGATCGACGGAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGACGACA‑TCTGGCTGGCTTCATTAACGCCTGCTATTCCCGTCAGCCTGAGCTTg                                                                                                                                                                                                    >  2:547494/1‑251 (MQ=255)                                     cattACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACACCATCAGCCAGCAATTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGTCTTCATTCACGCCTGCTATGCCCGTCAGCCTGAGCTTg                                                                                                                                                                                                    <  1:257350/250‑1 (MQ=255)                                      attACGACAACGTGGCCCCGTGTTTTCTTGGCGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTCAAGTCTCGTCGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTGTCGCCGCCAGGCTTGCAATGCGCACGGGCGTCA‑TCGGGCTGGCTTCCTTCACGCCTGATATTCCCGTCAGCCTGATCTTGcc                                                                                                                                                                                                  >  2:232839/1‑251 (MQ=255)                                      attACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGCCATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGCCA‑TCTGGCTGGCTTCCTTCACGCCTGCTATTCCCATCCGCCTGGGCttccc                                                                                                                                                                                                  >  2:303490/1‑248 (MQ=255)                                      attACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGcc                                                                                                                                                                                                  >  1:239528/1‑251 (MQ=255)                                             cAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGc                                                                                                                                                                                           >  1:447401/1‑251 (MQ=255)                                             cAACGTGGCACCGTGTTTTCTTGGGGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGAGCCAGGGTTTGATGAGTGGCTGTTGGTGCTGTCGTATCCGGGGATTAAAGACTCGACGGCAGAAGACAGGGCTATTTTACCGGCGCAGTATGGCCGCCTGGATTGCATTGATCACGGGC‑ACAATCTGGCTGGCTTCATTCACGCCTTCTATTCCCGTCAGCCTAGGCTTGcagcaaggg                                                                                                                                                                                           >  2:134337/1‑243 (MQ=255)                                                     cACCGTGTTTTCTTGGTGGTATGCAGGTGATGATCGAAGAAAACGACATCAGCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGaaa                                                                                                                                                                                   <  1:29444/251‑1 (MQ=255)                                                       ccGTGTTTTCTTGGTGGTATGCAGTTGATGATCGCAGAAAACGACATCATCAGCACGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGAATCCGGGGATTAAAGTCTCGACGGCAGAAGACAGGGCTCTTTTACCGGCGCAGTATCGCCGCCAGGATAGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCCGCCTGAGATTGCCGCGac                                                                                                                                                                                             >  2:407372/1‑238 (MQ=255)                                                       ccGTGTTTGCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGTCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGaa                                                                                                                                                                                             <  1:407372/239‑1 (MQ=255)                                                             tgtcttggtggtATGCAGTTGAGGATCGAAGAAAACGACATCAGCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCCGAGCTTGCCGCGAAGCTGATGAAAGATGTTa                                                                                                                                                                            <  1:445836/244‑1 (MQ=255)                                                                  tggtggtATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCt                                                                                                                                                                       <  1:387716/249‑1 (MQ=255)                                                                         aTGCAGGTGATGATTGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTa                                                                                                                                                               <  1:299480/251‑1 (MQ=255)                                                                                     atCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGTACGGtt                                                                                                                                                   >  1:197617/1‑251 (MQ=255)                                                                                        gaagaaAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACt                                                                                                                                                >  1:409996/1‑251 (MQ=255)                                                                                                 gACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCCCGCCTGCTCTTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTCCTGCCTGGCtt                                                                                                                                       >  2:31102/1‑251 (MQ=255)                                                                                                 gACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCtt                                                                                                                                       <  1:547494/251‑1 (MQ=255)                                                                                                  aCATCATCAGCCAGCAAGCGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTc                                                                                                                                      >  1:190210/1‑251 (MQ=255)                                                                                                   catcatCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTCCTGCCTGGCTTcc                                                                                                                                     >  1:608267/1‑251 (MQ=255)                                                                                                              agcAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCcggcaggcgcgg                                                                                                                          <  1:382460/251‑1 (MQ=255)                                                                                                                       cAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCggct                                                                                                                 >  1:278036/1‑249 (MQ=255)                                                                                                                             ttGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGa                                                                                                            <  1:452553/250‑1 (MQ=255)                                                                                                                                atggggtgCTGTGGGTGCGTGCGTACACGGGGATTAAAGGTTCGACGGCAGAAGCAAGGTGTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCAGTGAGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTAGCGCTGAACCCTTCCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATc                                                                                                        <  2:536996/244‑1 (MQ=255)                                                                                                                                atgaGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAGCCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATc                                                                                                        <  1:141789/251‑1 (MQ=255)                                                                                                                                  gaGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCg                                                                                                       <  1:633784/250‑1 (MQ=255)                                                                                                                                     tGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGGAATCGgcgg                                                                                                   >  1:17161/1‑250 (MQ=255)                                                                                                                                     tGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGGCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCcggcggctgaggacaacggcgc                                                                                                   >  1:352715/1‑235 (MQ=255)                                                                                                                                             ggTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGtttgggcaatcgggacgggcgacc                                                                                           >  2:612735/1‑228 (MQ=255)                                                                                                                                              ggacggaggaaTCCGGGGATTAAAGTGTCGACGGCTTAAGCCAGGTCTCTGTGCCCGGCTAATTATCGCCGCCAGGATTGCATTGCGCACGTGCGACA‑TCGGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTCAGCTTGCCGCGAAGCTGATGAAAGCTTTTATCGCGGAACCCTACCGTGAACGGTTCCTGCCTGGCTTCCGGCAGGCGCTGCAGGCGTTTGCGAAAATCGGCGAGGTagcgag                                                                                          <  2:28355/241‑1 (MQ=255)                                                                                                                                                  tGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGAATGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGACGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTGCTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGTGGaaaatgacgcggggagagaaggg                                                                                      >  2:387441/1‑231 (MQ=255)                                                                                                                                                      gTATCCGGGGATTAAAGTCCCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTAtct                                                                                  <  2:17161/251‑1 (MQ=255)                                                                                                                                                        aTCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGGAGCGGGCGGTATctcc                                                                                >  1:400180/1‑251 (MQ=255)                                                                                                                                                            ggggATTAAAGTCTCGACGGCAGAAGTCAGGGCTATTTTACCGGGGCAGTATCGCCGCCAGGATGGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTAGTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGGAGCGAGCGGTATctccggct                                                                            <  2:209199/251‑1 (MQ=255)                                                                                                                                                              ggatgaacggttcGACGGCAGAAGCCAGGGCTATTTTTCCGGCGCAGTAGCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATctccggctcc                                                                          <  2:400180/240‑1 (MQ=255)                                                                                                                                                              ggATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCCCGGTAGCGAGCGGGATctccggctcc                                                                          >  1:259656/1‑251 (MQ=255)                                                                                                                                                                          cGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGGAATCGGCGCGGGAGCGAGCGGTGTCTCCGGCTCCGGCCCGAccctt                                                              >  2:143563/1‑248 (MQ=255)                                                                                                                                                                               gCAGAAGCCCGGGCTATTTGACCGGCGCTGGATCGCCGCCAGGAGTGCTTTGCGCATGGGCGACA‑TCTGGCTGGCTTCATTCACGCCGGCTATGCCCGTCAGCCTGAGCTTGCTGCGAAGTTGATGAAAGATGTGAGCGCTGAACCCGACCGTGAACGGTTACTGCCTGGCTTCCGGCATGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCGCGACCGTGTTCGc                                                         <  2:611675/251‑1 (MQ=255)                                                                                                                                                                                    aGCCAGGGCTATTTTCCCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑GCTGGCTGGCTTCATTCTCGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCtgt                                                    <  2:580161/251‑1 (MQ=255)                                                                                                                                                                                            cTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTg                                                                                                                                                          >  1:480831/1‑141 (MQ=255)                                                                                                                                                                                            cTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑GCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTg                                                                                                                                                          <  2:480831/141‑1 (MQ=255)                                                                                                                                                                                                        cgcAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGcc                                <  1:612735/251‑1 (MQ=255)                                                                                                                                                                                                               ccgccgcCAGGATTTCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGcgcg                         <  2:239528/250‑1 (MQ=255)                                                                                                                                                                                                                      cAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGa                  <  1:199385/251‑1 (MQ=255)                                                                                                                                                                                                                      cAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATAGGCGCGGTATCGAGCGGTATCTCCGGCTCCGGCCCGACCTTTTTCGCTCTTTGAGACAAACCGGATACCGCCCAGCGCGTTGCCgc                  >  1:24179/1‑250 (MQ=255)                                                                                                                                                                                                                            tgcattgcGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCcgg                                                                                                                                   >  1:488172/1‑132 (MQ=255)                                                                                                                                                                                                                            tgcattgcGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCcgg                                                                                                                                   <  2:488172/132‑1 (MQ=255)                                                                                                                                                                                                                               attgcGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGGTACCGCCCAGCGCGTTGCCGACTGGTTggg         >  1:222923/1‑251 (MQ=255)                                                                                                                                                                                                                                ttgcGCACGGGCGACA‑GTTGGATGGCTTCAGTCAAGCCTGCTATTCCCGACAGCCTGAGTTTGCCGCGAAGCTGATGAAAGATGGTATCGCTGCACCCTGCCGTGAACGGTTACTGCCTGGCTGCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTggg         <  2:65022/250‑1 (MQ=255)                                                                                                                                                                                                                                  gcgcCCGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTACTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACATTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTaa      <  1:502385/251‑1 (MQ=255)                                                                                                                                                                                                                                      aCGGGCGAGA‑TCTGGTTGGGTTCATTCACGCCTGCTATCCCCGTCAGCCTGTGCTGTCCGCGAAGCTGATGAAAGATGTTAACGCGGAACCCGAGCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGGAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTTACAAGCCGGATACCGCCCAGCGCGTTGCCTACTGGTTGGGTAAGAAc  <  2:292297/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           ATGGGCGAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAAC  >  NC_000913/3179‑3658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑