Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,707,883	G→A	intergenic (‑178/+733)	dppA ← / ← proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,707,883	0	G	A	100.0%	156.3 / NA	49	intergenic (‑178/+733)	dppA/proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/26);  total (23/26)

CGACGGTCATAGCCACCAGGCTGAGACCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACG  >  NC_000913/3707639‑3708120                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              cGACGGTCATAGCCACCAGGCTGAGACCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAAc                                                                                                                                                                                                                                          >  1:316887/1‑250 (MQ=255)        cATAGCCACCAGGCTGAGACCAATCTTCAGCTTCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTTTAGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCGCTCTTGACCTACAATCTATCAACAGAAtgt                                                                                                                                                                                                                                   <  1:532530/250‑1 (MQ=255)             ccaccaGGCTGAGACCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAAc                                                                                                                                                                                                                             >  1:443012/1‑251 (MQ=255)                                             ggcTTTTTCTAGGAAATACGCATTATTCTGCTCCAATTGGGATGTTTGTTGTTTTAACCCGTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAAc                                                                                                                                                                                              <  2:147310/248‑1 (MQ=255)                                                               cGCATTATTCTGCTCCAATTGTGATGTGTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTGTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCacaaacaa                                                                                                                                                                           <  1:106300/251‑1 (MQ=255)                                                                gCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCca                                                                                                                                                                                  >  1:216950/1‑243 (MQ=255)                                                                gCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTGTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGAAGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTGACACCAACGGTGAGAATCca                                                                                                                                                                                  <  2:216950/243‑1 (MQ=255)                                                                  attattCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACTAACGGTGAGAATCCacaaacaa                                                                                                                                                                           >  1:111524/1‑248 (MQ=255)                                                                    tattCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGAt                                                                                                                                                                       <  2:316887/250‑1 (MQ=255)                                                                     attCTGCTCCAATTGTGCTGTTTTTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTAGGGTCAACGTTATGAGGTGGGGATGCCGACCTAATTAACATCAGTGTAACAGTGCGGAACCTACATAAAAGGCCCCTCGGGAACTACAATCTATAAACAGACTGTGAAAACGACAATACAGATACCCGGGATTTAAACCGAAGGGCAGAATCcaccaacagagatt                                                                                                                                                                      >  2:382928/1‑239 (MQ=255)                                                                      ttCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTa                                                                                                                                                                     >  1:186049/1‑250 (MQ=255)                                                                                 ttGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGCAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCa                                                                                                                                                          >  2:231398/1‑250 (MQ=255)                                                                                  tgtgATGTTTGTTGTTGTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCaa                                                                                                                                                         <  1:423411/250‑1 (MQ=255)                                                                                    tgATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAAc                                                                                                                                                       >  1:249188/1‑250 (MQ=255)                                                                                    tgATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAAc                                                                                                                                                       >  1:336433/1‑250 (MQ=255)                                                                                      aTGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGc                                                                                                                                                    >  2:208875/1‑251 (MQ=255)                                                                                      aTGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGc                                                                                                                                                    >  1:108471/1‑251 (MQ=255)                                                                                        ttttgttGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCTTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCt                                                                                                                                                   <  1:604913/249‑1 (MQ=255)                                                                                        gtttgttGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCt                                                                                                                                                   >  2:194615/1‑250 (MQ=255)                                                                                            gttgttTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTAttt                                                                                                                                               <  2:108471/250‑1 (MQ=255)                                                                                                   tAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCTGTCGGGTCAACGTTATGAGGTGGGTATTCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGTATTTACACCAACGGTGAGAATCCACCAACAAAGATTAAAAAAACTTCAAACAGCTATTTgcagc                                                                                                                                          >  2:72781/1‑248 (MQ=255)                                                                                                       ccTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACAACAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGaaa                                                                                                                                   <  2:278906/251‑1 (MQ=255)                                                                                                          ttGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACAGCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAAGCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAAt                                                                                                                                  <  2:443012/249‑1 (MQ=255)                                                                                                                 gggTTGTCGCGGCCTGTCCTGTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGAGCCTCCATAAAATGCCCCTCGTGACCTACAATCTAACAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGATAATCCACAAACGAAGATTAAAAAAACTTCAGACAGCTATTTGCTGCAGACAAATTGGTGCTAc                                                                                                                         <  2:330355/251‑1 (MQ=255)                                                                                                                     tGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGAAGCAGAGAAATTTGTGCTACTcc                                                                                                                      >  1:567758/1‑250 (MQ=255)                                                                                                                         gCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACAt                                                                                                                 <  2:140023/251‑1 (MQ=255)                                                                                                                            gCCTGACCTTTTTTGTTTTGTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGAc                                                                                                              <  1:85137/251‑1 (MQ=255)                                                                                                                                gatcgttggttttttttgCCCGGGCGGTTAAACGTTAGGCGGTGGGGATGCCTTACTACTTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTTCAATCGATCGACAGTATGTTAAAACTTCAATACAGCCAACAGGGAGTTACTCCAACGGTGAGAATCTCCAAACAAAGAGTACAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCGACTCCAACATTACCAg                                                                                                           <  2:401429/239‑1 (MQ=255)                                                                                                                                     tttttgttttttgCCCGTTTGTGGCAACGTTATGAGGTGTGGATGCCGTACTAATTGACATCAGGGTACCAGTACGGATCCTCCATAAAACGCCCCTCGTGACCTACAATCTATCAACAGAATTTGAAAACGTCAATACAGCCAACCGGGATTTACAACAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACACCAACATGCCCAGAACa                                                                                                       <  2:310714/249‑1 (MQ=255)                                                                                                                                       tttgttttttgCCCCGTCGGGTCAACGTTATGAGGTGGGGATGACCTACTAATTAACATCAATGTGACAGTACGGATCTTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGGCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTTCCACATGACCATATCAATc                                                                                                    <  2:45536/250‑1 (MQ=255)                                                                                                                                          gttttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGc                                                                                                 <  1:206515/250‑1 (MQ=255)                                                                                                                                              tttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTaa                                                                                             <  1:307630/250‑1 (MQ=255)                                                                                                                                                             cAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTg                                                                             <  1:72781/251‑1 (MQ=255)                                                                                                                                                                   tATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGAtt                                                                       >  1:151652/1‑251 (MQ=255)                                                                                                                                                                      gAGGTGGGGATTCCGTCCTAATTAACATCAGTGGAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCCTCAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATAATTAGCAACATTTGGTGATTAAg                                                                    <  2:487337/251‑1 (MQ=255)                                                                                                                                                                      gAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAg                                                                    <  1:511106/251‑1 (MQ=255)                                                                                                                                                                           ggggATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGtttt                                                                >  1:103426/1‑250 (MQ=255)                                                                                                                                                                            gggATGCCGTACTAATTAACATCAGAGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCTAACCGGGATTTACACCAACGGTGAGAATCCCCAAACAAAGATTAAAAAAACTTCAAACAGCTAGTTGCAGCAGAGAAATTTGTGCTACTTCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTAt                                                              <  2:336433/251‑1 (MQ=255)                                                                                                                                                                                      aCTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATg                                                    >  1:219562/1‑251 (MQ=255)                                                                                                                                                                                                 tCAGTGTAACAGTGCGGATCCTACATAAAATGCCCCTCGTGACCTACAATCTATAAACAGAATGTGAAAAAGTCAATACAGCCAAACGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAACAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACAAGAACAATCAGCTTAATATTTAGCAAAATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTg                                         >  2:306847/1‑251 (MQ=255)                                                                                                                                                                                                       tAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACAAGAACAATCAGCTTACTATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAACAAATCTGAGGaa                                    >  1:667688/1‑250 (MQ=255)                                                                                                                                                                                                                ggATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGata                          <  2:186049/251‑1 (MQ=255)                                                                                                                                                                                                                  aTCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTAATCAAACATGACCAGAACAATCAGCTTAAAATTTAGCAACATTTGGTGATTAAGTTTTATGAATTTAATGAAAAAAATCTAAGGAGAAgggtgatatt                        >  2:164534/1‑243 (MQ=255)                                                                                                                                                                                                                            aaaTGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACAtt                                                                               <  2:254817/186‑1 (MQ=255)                                                                                                                                                                                                                            aaaTGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACAtt                                                                               >  1:254817/1‑186 (MQ=255)                                                                                                                                                                                                                             aaTGCCCCTCGTGACCTAAAATCTATCAACAGAATGTGAAAACGTCAATACCGCCAACCGGGATTTACACCAACGGTGAGAATCCACAACCAAAGATTAAAAAAACTTCAAACAGCTATTTGCCGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAAAATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGTGCGATTa              >  2:566187/1‑250 (MQ=255)                                                                                                                                                                                                                                gCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATAc          <  1:359111/251‑1 (MQ=255)                                                                                                                                                                                                                                       gTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAAc   >  2:365451/1‑251 (MQ=255)                                                                                                                                                                                                                                         gACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACg  <  1:55440/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              CGACGGTCATAGCCACCAGGCTGAGACCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACG  >  NC_000913/3707639‑3708120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑