Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,244	C→T	L148L (CTC→CTT)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,244	0	C	T	100.0%	219.8 / NA	70	L148L (CTC→CTT)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/27);  total (43/27)

GCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAA  >  NC_000913/3003‑3481                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               gcggggAGCGTTTGTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATg                                                                                                                                                                                                                                       <  2:287581/248‑1 (MQ=255) gCTGGGATCGTTTTTGCCCGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTTGAAAAGAATATGCCAATCGGTTCGGGCGTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGTGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATg                                                                                                                                                                                                                                       <  2:21446/251‑1 (MQ=255)     ggaggttttgtgcaaggagcttggTAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGATTTAGGCTCCAGCTCCTGTTCGGTGGTCGCGGCTCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGt                                                                                                                                                                                                                                   <  2:486787/229‑1 (MQ=255)          gTTTTTGACAGGAACTTGGCAAGCAAATTCCCGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGGTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGAAGAATCTCCGGCAGCATTCATTACGACAGCGTGACACCTGGTTTTCTTGGTGGAATGCAGTtgatg                                                                                                                                                                                                                              >  2:615022/1‑251 (MQ=255)            ttttGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTtgatga                                                                                                                                                                                                                             <  1:591318/250‑1 (MQ=255)                 ccAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGATGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCga                                                                                                                                                                                                                         >  1:434613/1‑249 (MQ=255)                 ccAGGAACTTGGAAAGCAACTTCCAGTGGCGATGACTCTGGTAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGCATGAACAATGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTTGCAGGACGAATCTCCGGCAGCATTCATTACGCCAACGTGGCACCTTGTTTTCTTGGAGGTATGCAGTGTATGATCga                                                                                                                                                                                                                         >  2:248952/1‑249 (MQ=255)                             caaGCAAATGCCAGTGGCGATGACTCTGGAAAATAATATGCCAAGCGGTTCGGGCTTAGGCTCCAGCGCCTGTGCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCGTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGAcatca                                                                                                                                                                                                           <  2:453374/250‑1 (MQ=255)                              aaGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTGGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGCTGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGAcatcat                                                                                                                                                                                                          >  1:566781/1‑251 (MQ=255)                                gCAAATTCCAGTGGCGATGACTCTGGAGAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGCACACTGTGGCCAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGACTCTCCGGCAGCAGTCATTAGGACTACGTGGCACCGTGTTTTCTTGGAGGAATGCAGTTGATGATCGAAGAAAACGAcgacatc                                                                                                                                                                                                         >  2:387716/1‑244 (MQ=255)                                         aGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCa                                                                                                                                                                                                 >  1:209199/1‑249 (MQ=255)                                           tGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGCCGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGGGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCaaagg                                                                                                                                                                                             >  2:137276/1‑248 (MQ=255)                                           tGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCaa                                                                                                                                                                                                <  1:137276/248‑1 (MQ=255)                                            ggCGATGACTCTGGAAAAGACTATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGc                                                                                                                                                                                            >  1:487224/1‑251 (MQ=255)                                            ggCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGc                                                                                                                                                                                            <  1:449166/251‑1 (MQ=255)                                               gATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGAGCTGATGGCGATGAATGAAAACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGCGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAAAGTGGCACCGAGTTTTATTGGTGGTATGCAGTTGATGATCGAAGAAAAAGACATCATCAGCCAGAAAGTGcc                                                                                                                                                                                           >  2:496142/1‑249 (MQ=255)                                                        ggAAAAGAATATGCCAATCGGGTCGGGCTTAGGCTCCAGCGGCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTtga                                                                                                                                                                                  <  2:321868/249‑1 (MQ=255)                                                         gAAAAGAATGTGCCAATCGGTTCGGGCTTAGGCCCCAGCGCTTTGTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACGCTGTGGCAAGCCTCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGTGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCTAGTGCCAGGGTTtgatga                                                                                                                                                                               <  2:434613/251‑1 (MQ=255)                                                                   tGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTgg                                                                                                                                                                     >  1:611675/1‑251 (MQ=255)                                                                     ccactCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTgggg                                                                                                                                                                   >  2:199385/5‑250 (MQ=255)                                                                               cGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTAt                                                                                                                                                         >  1:292297/1‑251 (MQ=255)                                                                               cGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGACGAGCTGGAAGGACGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGAGGCTGTGGGTGCTGGCGTAt                                                                                                                                                         >  1:117678/1‑251 (MQ=255)                                                                                 ggCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATc                                                                                                                                                                                                                           >  2:121763/1‑183 (MQ=255)                                                                                 ggCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATc                                                                                                                                                                                                                           <  1:121763/183‑1 (MQ=255)                                                                                   cTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCgg                                                                                                                                                     >  1:536996/1‑251 (MQ=255)                                                                                      aGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCgggg                                                                                                                                                   <  1:381404/250‑1 (MQ=255)                                                                                          tCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGGGTGGCTGTGGGTGCTGGCGTATCCGGGGATTa                                                                                                                                               >  1:281530/1‑250 (MQ=255)                                                                                                gCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGtctc                                                                                                                                        <  1:70200/251‑1 (MQ=255)                                                                                                    gTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGAGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACg                                                                                                                                    >  1:100356/1‑251 (MQ=255)                                                                                                         gtggtCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGGTTAAAGTCTCGACGGc                                                                                                                                  >  2:139006/1‑248 (MQ=255)                                                                                                         gggggcgcgGCGCTGAGGGCGAATAAAGAACTCTGTGGCAAGCCGCTGAAGGACACGCGTTTGCTGGCTTTGATGGGCGTGCTGGAAGGACTAATCTCCGGCAGCAGTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGa                                                                                                                               <  2:566781/246‑1 (MQ=255)                                                                                                           ggtCGCGGCGCTGAGGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGTAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGaa                                                                                                                              <  1:396453/250‑1 (MQ=255)                                                                                                            acccgtcgctcgtccgcccttaatgaaCACTGGGGCAAGCCGCTGAAGGACACTCGTTTGCTGGCTTGGATTGGCGAGCTGGAAGGACGAATCTCCGGCAGCATGCATTACGACAACGGGGCTCCGGGTTTTCGTGGTGGTATGCAGGTGAGGATCGAGGAAAACGACATCATCAGCCAGCAAGGGCCAGGGTTGGATGAGTGGATGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGc                                                                                                                            <  2:498031/230‑1 (MQ=255)                                                                                                              cgcgGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCa                                                                                                                          <  1:615022/251‑1 (MQ=255)                                                                                                                cggcgCTGATGGCGCTGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGGTGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAACGTCTCGACGGTAGacaccagg                                                                                                                        >  2:633784/1‑244 (MQ=255)                                                                                                                   cgcTGATGGCAACGAATGAACCCTGAGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGCTGGGCGAGCAGGAAGGACGAATCTCCGGCAGCATTCATTACGACAGCGTGGCACCGTGTGTTCTTGGCGGGATGAGGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGGGCAAGGGTTTGATGAGTGGCTGGGGGTGCTGGAGTATCCAGGGATTAACGTCGCAACGGCAGACGCCGGGGCt                                                                                                                     >  2:439399/1‑251 (MQ=255)                                                                                                                      tGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTAtt                                                                                                                  >  1:237184/1‑251 (MQ=255)                                                                                                                       gagggCGATGAATGAACACTGTTGCAAGCCGCTTAATGACACCTGTTTTCGGTCTTTGATGGGCGAGCGTGAAGGACGAATCTCCGGCAGCATTCATTCCGACAACGTTGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTAt                                                                                                                   <  2:46403/246‑1 (MQ=255)                                                                                                                        atgtcGATGAATGAACACTGTGGCAAGCCGCTTAAGGACACTCGTTTGCTGGCTTTTATGGGCGAGATGGAAGGACGAATCTCCGGCAGCATTCATTACTACAACGTGGCAACGTGTTTTCTTGGTGTTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTAgttt                                                                                                                <  2:100356/247‑1 (MQ=255)                                                                                                                                gaatgaaCACTGTGGCAAGCCGCTTAATGACCCTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGc                                                                                                                            >  1:498031/1‑231 (MQ=255)                                                                                                                                        acTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATcgc                                                                                                >  1:557738/1‑251 (MQ=255)                                                                                                                                                   ccGCTTAATGACCCTCGGTTGCTGGCTTTGATGGGCTAGCTGGAAGGACGATTCTCCGGCAGTAGTCATTACGACAACGTGGCACCGTGTTGTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGACAGGattg                                                                                     <  2:487224/251‑1 (MQ=255)                                                                                                                                                         aaTGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGattgcattgc                                                                               >  1:345240/1‑251 (MQ=255)                                                                                                                                                          aTGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTgcg                                                                              >  1:580161/1‑251 (MQ=255)                                                                                                                                                            gACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCAAGGCTTGCATTgcgc                                                                             >  2:259432/1‑250 (MQ=255)                                                                                                                                                               acTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTCTCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTTCGCACgg                                                                         >  2:382460/1‑251 (MQ=255)                                                                                                                                                                        cTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTAATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCt                                                               >  1:573873/1‑251 (MQ=255)                                                                                                                                                                        cTGGCTTTGAAGGTCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTAGGAGAACGAGGCACCGTGTTTTCTTGGTGGTATGCAGTTGGTGATCGCAGACAACGACATCATCAGCCAGCGAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGAGTCCGGGGAATAACGTCTCGACGGCAGAGGCCAGGGCTATTTTACCGGCGTAGTATCGACGCCAGGAATGCAGTGCGCACGGGCGGCA‑TCt                                                               >  2:330807/1‑251 (MQ=255)                                                                                                                                                                          ggCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCAGTACGACAACGTGGCACCGTGTGTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCATGGCTAGTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCt                                                               <  1:34332/249‑1 (MQ=255)                                                                                                                                                                            cattGATGGGCGAGCTGGAAGGACGAATATCCGGAAGCATTCATTACGACAACGTGGCACCGTGGTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGATTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGAATCGCCGCCAGGATTGCATTGCGAACGGGCGACA‑TCTGGct                                                           >  1:170117/3‑250 (MQ=255)                                                                                                                                                                                gATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACATCGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGct                                                           <  1:343255/247‑2 (MQ=255)                                                                                                                                                                                gATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCCCGACGGCAGCAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGACTGCATTGCCCACGGGCCATA‑TCTGGcttgat                                                       >  2:343255/1‑246 (MQ=255)                                                                                                                                                                                 aTGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCtt                                                      <  1:259432/251‑1 (MQ=255)                                                                                                                                                                                                          ccGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGGTTGCATTGCGCCCGGGCGACA‑TCTGGCTGGCTTCATTCCAGCCTGCTATTCCCGTCac                             >  2:218711/1‑250 (MQ=255)                                                                                                                                                                                                          ccGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTc                               >  2:141789/1‑249 (MQ=255)                                                                                                                                                                                                             gcagcaTGCATTCAGACAACTTGGCACCGTGTTTTCTTGGTGGTATGCGGTGTATGATCGAAGAAAACGACATCAGCATCCAGCAAGTGCCAGGTTTTGGTGAGTGGCTGTGTGTGCTGTCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGTCTATTTTACCTGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGGTTCATTCACGCCTGCTATTCCCGTCAGCCt                          <  2:183510/251‑1 (MQ=255)                                                                                                                                                                                                                 cattcattACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGc                      >  1:416242/1‑251 (MQ=255)                                                                                                                                                                                                                 cattcattACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGc                      >  1:582811/1‑251 (MQ=255)                                                                                                                                                                                                                    tcattACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCtt                    <  1:496142/250‑1 (MQ=255)                                                                                                                                                                                                                    tcattACGACAACGTGGCACCGTGTTTTCTTGGGGGTATGCAGTTGATGATCGACGGAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGACGACA‑TCTGGCTGGCTTCATTAACGCCTGCTATTCCCGTCAGCCTGAGCTTg                   >  2:547494/1‑251 (MQ=255)                                                                                                                                                                                                                     cattACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACACCATCAGCCAGCAATTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGTCTTCATTCACGCCTGCTATGCCCGTCAGCCTGAGCTTg                   <  1:257350/250‑1 (MQ=255)                                                                                                                                                                                                                      attACGACAACGTGGCCCCGTGTTTTCTTGGCGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTCAAGTCTCGTCGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTGTCGCCGCCAGGCTTGCAATGCGCACGGGCGTCA‑TCGGGCTGGCTTCCTTCACGCCTGATATTCCCGTCAGCCTGATCTTGcc                 >  2:232839/1‑251 (MQ=255)                                                                                                                                                                                                                      attACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGCCATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGCCA‑TCTGGCTGGCTTCCTTCACGCCTGCTATTCCCATCCGCCTGGGCttccc                 >  2:303490/1‑248 (MQ=255)                                                                                                                                                                                                                      attACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGcc                 >  1:239528/1‑251 (MQ=255)                                                                                                                                                                                                                             cAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGc          >  1:447401/1‑251 (MQ=255)                                                                                                                                                                                                                             cAACGTGGCACCGTGTTTTCTTGGGGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGAGCCAGGGTTTGATGAGTGGCTGTTGGTGCTGTCGTATCCGGGGATTAAAGACTCGACGGCAGAAGACAGGGCTATTTTACCGGCGCAGTATGGCCGCCTGGATTGCATTGATCACGGGC‑ACAATCTGGCTGGCTTCATTCACGCCTTCTATTCCCGTCAGCCTAGGCTTGcagcaaggg          >  2:134337/1‑243 (MQ=255)                                                                                                                                                                                                                                     cACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACg                                                                                                                                    >  1:146745/1‑122 (MQ=255)                                                                                                                                                                                                                                     cACCGTGTTTTCTTGGTGGTATGCAGGTGATGATCGAAGAAAACGACATCAGCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGaaa  <  1:29444/251‑1 (MQ=255)                                                                                                                                                                                                                                       ccGTGTTTTCTTGGTGGTATGCAGTTGATGATCGCAGAAAACGACATCATCAGCACGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGAATCCGGGGATTAAAGTCTCGACGGCAGAAGACAGGGCTCTTTTACCGGCGCAGTATCGCCGCCAGGATAGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCCGCCTGAGATTGCCGCGac            >  2:407372/1‑238 (MQ=255)                                                                                                                                                                                                                                       ccGTGTTTGCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGTCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGaa            <  1:407372/239‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               GCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACA‑TCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAA  >  NC_000913/3003‑3481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑