Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,500,129	G→A	G74S (GGC→AGC)	frlA →	fructoselysine/psicoselysine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,500,129	0	G	A	93.3%	75.3 / ‑1.4	30	G74S (GGC→AGC)	frlA	fructoselysine/psicoselysine transporter
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (9/19);  total (10/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01

GGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGGCGGCGCAGCGTTTCAGACGCT  >  NC_000913/3499901‑3500373                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     gggagcGCTATGGGATGCAATGAACTCTACCTCGAGCGCGGATTTTGGGCCGTTCTTGCAATCGCCGGCGGGACACCCGTCGGCTCCGGTATTGTTGTATCTGTGGGTGAATTGGCAAAAGAAGCGGGCACGTCGTGGCTTACGGTGCTCGCGTTTGTCATTTGCGGGTTAATTTTGCTCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTGTGTTTATCTGaa                                                                                                                                                                                                                                <  2:20469/246‑1 (MQ=255)             ggAAGCCAGCAACTCCATCGCATGCCCGGATTTTGGGCCTTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTCTTTTTGTATCTGTGGTTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCAGGAAg                                                                                                                                                                                                                    <  1:176738/251‑1 (MQ=255)                  ccAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGTTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGAc                                                                                                                                                                                                               <  1:98940/251‑1 (MQ=255)                  ccAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGAc                                                                                                                                                                                                               <  1:441472/251‑1 (MQ=255)                   cAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGGTAATTTGGATCCCCCAAATGTGCGGCTTTGCGGAACTAATCACCGCTTTTCCGGAAAATAGAGCAGATTATGTTTATTTGGAAAATGCCGGAAGCCGAcc                                                                                                                                                                                                              >  1:530589/1‑251 (MQ=255)                        aCTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTgg                                                                                                                                                                                                         <  1:277535/251‑1 (MQ=255)                        aCTCCAACGCAAGCTCGGATGTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTGATCTGAAAAATGCCGGAAGCCGACCGCTgg                                                                                                                                                                                                         <  1:498733/251‑1 (MQ=255)                            cAACGCAAGCTCGGTTTTTGGGCCGTTCTTGCATGCGCCGTCGGGAACTCCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGTCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAACTAGCGCAGATTATGTATATCTGACAAATGCCGGAAGCCGTCCGCTGGCttt                                                                                                                                                                                                     <  2:339669/251‑1 (MQ=255)                                                   cGTTGTTGCAATCGCGGTCGGGACAACAGTCTGCTCCGGTAGTTTTGTATCTGGGGGTTAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTGACGGTGCGCGCGTTTGTCAGTGGCGGGTTAATTGTGATCCCGCAAATGTGCGGCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGTCCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCt                                                                                                                                                                              <  2:636556/251‑1 (MQ=255)                                                               cGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCTGCTGGGCCAGCTTCTGGGCCAACGAt                                                                                                                                                                   <  1:501145/250‑1 (MQ=255)                                                               cGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGAt                                                                                                                                                                   <  1:131813/250‑1 (MQ=255)                                                                           aaCCGTCGGCTCAGGTATTTTTGTATCTGTGGGTGAAGGGGCAAAAGCAGCGGGCCAGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCTCCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGt                                                                                                                                                      <  1:413928/251‑1 (MQ=255)                                                                                 cGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCGCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGAtt                                                                                                                                                 <  1:240836/250‑1 (MQ=255)                                                                                             ttttGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGGTGATTTTGATTCCCCCAATGTGCGGCTTTGCGGAACTTTCCCCCCCTTATCCGGAAAATAGCGCAGGTTATTTTTTTCTTAAAAATACCGGGAGCCGACCCCCGGGTTTCCTCTCCCGCTGGGCCCGCATCTGGGCCAACGAAGCGCCCTCATTGtgggtttttgggctggcgg                                                                                                                                    >  1:498829/1‑233 (MQ=255)                                                                                                                                 ccctccGTGGCTTACTGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGCCTATGCGTAACTATCCACCCCTTATCCGGAAAATAGCTCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGTCCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCAGTGTCGATTATGGCGCTGGCGACTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATc                                                                                                <  1:329045/247‑1 (MQ=255)                                                                                                                                                                    gCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGattaatt                                                             >  1:181740/1‑251 (MQ=255)                                                                                                                                                                      gggTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTAt                                                           <  1:109698/251‑1 (MQ=255)                                                                                                                                                                        gTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCg                                                         <  1:384900/251‑1 (MQ=255)                                                                                                                                                                        gTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCg                                                         <  1:385321/251‑1 (MQ=255)                                                                                                                                                                             ttGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTc                                                                                                                               <  1:50605/176‑1 (MQ=255)                                                                                                                                                                             ttGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTc                                                                                                                               >  2:50605/1‑176 (MQ=255)                                                                                                                                                                                 gATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGCTTAATTATCGCCTTTATGt                                                >  2:478883/1‑251 (MQ=255)                                                                                                                                                                                  aTCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGtt                                               <  1:295743/251‑1 (MQ=255)                                                                                                                                                                                      cGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTa                                           <  1:18606/251‑1 (MQ=255)                                                                                                                                                                                                               aTCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGgcggcg                  >  1:670227/1‑251 (MQ=255)                                                                                                                                                                                                                       cTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGGCGGCGCAGCGttt          >  1:184862/1‑251 (MQ=255)                                                                                                                                                                                                                         tATCCGGAAAATAGCGAAGATTATGATTATCTAATAAATACCGGAAGCCGACAGATGGCTTTCATCTCCGGCTCGGCAAGCTTCTGGGCGAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCATCAATATTGGCTTTTTAACGCTTATCGTTCCGTTGCTCGGTAAATTTATCGCCGACGGATTAATTATCGACTTTAAGTTGATAAACCTGCGCTCAGTTGAAGGCGGCGCAGTGTTTc         >  2:479496/1‑250 (MQ=255)                                                                                                                                                                                                                            ccGGAAAATGGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCg                                                         <  2:45096/199‑1 (MQ=255)                                                                                                                                                                                                                            ccGGAAAATGGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCg                                                         >  1:45096/1‑199 (MQ=255)                                                                                                                                                                                                                               gAAAATAGCGAAGCTTCTGTTTATCTGAAAAATGCCGGAAGCCGCCCGCTGGATTTCCTCTACGGCTGAGCCAGATTCTGGGCCAACGCTGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGACTTTATGTTGCTACACCTGCGCTCCGTTGAAGGCGGAGCAGCGTTTCCGACGcc  >  2:643858/1‑250 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     GGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGGCGGCGCAGCGTTTCAGACGCT  >  NC_000913/3499901‑3500373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑