Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,502	C→T	T128I (ACT→ATT)	crp →	DNA‑binding transcriptional dual regulator CRP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,502	0	C	T	100.0%	149.6 / NA	47	T128I (ACT→ATT)	crp	DNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/23);  total (24/23)

CAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCT‑GTTTGAAGAGGGCCAGGAA‑CGTAG‑CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGG  >  NC_000913/3486265‑3486742                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 agtgacTGAACAAAAACGAAGAGTGAAAAAAAAGGATCCTTTCCTATCGGAACCGGGGTAATTTTATTGGCGAACGGGGCCT‑TTTTGAAGGGGGCCAGAAA‑CGTAG‑CGCATGGGTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTg                                                                                                                                                                                                                                     <  2:36015/246‑1 (MQ=255)                 ggaggggggaaaaaaaaTGTTCCTCTCCTATCTAAATGAGGGGGTTTTTATTGGGGAA‑GGGGCCTTGTTGGAAGAGGGC‑AGGAAAGGTAG‑GGTAGGGGTAGTTGGAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTcc                                                                                                                                                                                                                     <  2:9185/237‑1 (MQ=255)                       gggTAAAGATATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCT‑GTTTGAAGAGGGCCAGGAA‑CGTAG‑CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACg                                                                                                                                                                                                               >  2:609495/1‑251 (MQ=255)                       gggTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCT‑GTTTGAAGAGGACCAGGAA‑CGTAG‑CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGCTGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGaaa                                                                                                                                                                                                                                        >  2:4855/1‑226 (MQ=255)                                 aTGATCCTCTCCTATCTGAATCAGGGTGCTTTTATTGGCGAACTGGGCCT‑GTTTGAAGAGGGCCAGGAA‑CGTAG‑CGCATGGGTACGGGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGCCATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCACGTCATTTCAGAGAAAGTGTGCAACATGGCGATCCTCGACGTGACGGGCCg                                                                                                                                                                                                     >  1:469026/1‑251 (MQ=255)                                     cccgattttATCGGAAGCAGGGTGTTTTTTTTGGCGAACGGGGCTT‑GTTGAAAGAGGGCCAGGAC‑CGTTG‑CGCATGGTTACGGGAAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCAtt                                                                                                                                                                                                 <  1:125667/243‑1 (MQ=255)                                      ccTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCT‑GTTTGAAGAGGGCCAGGAA‑CGTAG‑CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGCCGTGACGGGCCGCATTg                                                                                                                                                                                                >  1:664238/1‑251 (MQ=255)                                        ccccttcttggactcggggTTATTTTAGTGGCGGACGGGGCCT‑TGTGGAAGAGGGCCAGGAA‑TGAAG‑CGCATGTGTACGTTCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTGTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGca                                                                                                                                                                                              <  1:640920/235‑1 (MQ=255)                                         ctcCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCT‑GTTTGAAGAGGGCCAGGAA‑CGTAG‑CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGcac                                                                                                                                                                                             >  1:349007/1‑251 (MQ=255)                                             tATCTGAATCAGGGTGCTTTTATTGGCGAACTGGGCCT‑GTTTGAAGAGGGCCAGGAA‑CGTAG‑CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGCTTCAGGTAAGCCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGATAGTGGGCAACCTGACGTTCCTCGACGTGACGGGCAGCAtt                                                                                                                                                                                                 >  2:125667/1‑243 (MQ=255)                                               tCTGAAACAGGGAGATTTTTTTGGCGAAAAGGGCCT‑CTTTGACAAGGGCCAGGAA‑CGTAG‑CGCATGAGTACGTTCGAAAAACGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAAGTAACCCCGGACATTCTGATGCGTTTGTCTGCACAGATGACGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCCACCTGGCGTTCCTCGACGTGCCGGGTCTCATAGCACAGActc                                                                                                                                                                                       >  2:415495/1‑251 (MQ=255)                                                 gaaatgggggTGATTTTATGGGCAAACGGGGCCT‑TTTTGAAG‑GGGCCAAGGA‑CTTAGGCGAATGGTTCCGGGAAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTctg                                                                                                                                                                                     <  2:136315/244‑1 (MQ=255)                                                  gAATCGGGGTGTTTTTATGGGCGAACGGGGCCT‑TTTAGAAGGGGCCCAGGAC‑CGTAG‑CCGATGGGTACGTGAAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTctg                                                                                                                                                                                     <  1:318957/250‑1 (MQ=255)                                                    aTCAGGGTGATTTTATTGGCGAACTGGGCCT‑GTTTGAAGAGGGCCAGGAA‑CGTAG‑CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCCTTGCACAGACTctgctg                                                                                                                                                                                  >  2:159426/1‑251 (MQ=255)                                                               tttATTGGCGACCGGGGCTT‑TTTTGAAGAGGGCCAGAAA‑CGTAG‑CGCAGGGTTACGGGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTctg                                                                                                                                                                                     <  1:459653/237‑1 (MQ=255)                                                               tttATTGGCGAACTGGGCCT‑GTTTGAAGAGGGCCAGGAA‑CGTAG‑CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCAGTGCACAGACTctg                                                                                                                                                                                     >  2:459653/1‑237 (MQ=255)                                                               tgtagtggagaattggtccT‑GTTAGAAGAGGGCCAGGAA‑CGTAG‑CGCATGGGTACGTGAAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCaa                                                                                                                                                                        <  1:644632/233‑1 (MQ=255)                                                                     gtcGAACGGGGCGT‑GGTTGAAGAGGCAAAGGAA‑CGTAT‑CGTTTGGGTTCGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAAcc                                                                                                                                                                 <  1:472151/249‑1 (MQ=255)                                                                        cgacgggggctttttggAAGAGGGCAAGGAC‑CGTAG‑TGCAGGGGTCCGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAg                                                                                                                                                               <  1:441466/235‑1 (MQ=255)                                                                           cggggCCT‑GTTTGAAGAGGGCCAGGAA‑CGTAG‑CGCATGGGTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAgaga                                                                                                                                                                                                                                          <  1:29990/170‑1 (MQ=255)                                                                           cTGGGCCT‑GTTTGAAGAGGGCCAGGAA‑CGTAG‑CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCagagc                                                                                                                                                                                                                                          >  2:29990/1‑171 (MQ=255)                                                                                  t‑GTTTGAAGAGGGCCAGGAA‑CGTAG‑CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACt                                                                                                                                                    >  1:617011/1‑251 (MQ=255)                                                                                       ggAAGAGGGCCAGGAA‑CGTAG‑CGCATGGTTACGTGAAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAcc                                                                                                                                                <  1:187193/250‑1 (MQ=255)                                                                                                  aGGAA‑CGTAG‑CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAACTTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCACAGCAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGTCTCTGCTGAATCTGGCAAAACAACCAGACGCTATTACTCACCCGGACGGTATg                                                                                                                                     >  1:80108/1‑251 (MQ=255)                                                                                                                        gtttcaaatcccGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGTATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATAAAAATTCCCCGTCa                                                                                                                 <  1:245607/241‑1 (MQ=255)                                                                                                                            cGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAaaca                                                                                                                                                                    >  1:343085/1‑196 (MQ=255)                                                                                                                               aaaCCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAAtt                                                                                                          >  1:505212/1‑251 (MQ=255)                                                                                                                                   cGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAATCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCTTGCCTCGACGTGACGGGCCGCATTGCACAGACTCGGCTGAATCTGGCAAAACATCCAGACGCTATg                                                                                                                                                       <  2:414656/202‑1 (MQ=255)                                                                                                                                   cGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATg                                                                                                                                                       >  1:414656/1‑202 (MQ=255)                                                                                                                                                gCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTg                                                                                         >  1:82248/1‑251 (MQ=255)                                                                                                                                                              cAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCa                                                                                                                                   >  1:156028/1‑195 (MQ=255)                                                                                                                                                              cAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCa                                                                                                                                   <  2:156028/195‑1 (MQ=255)                                                                                                                                                                         gCCAATTGATTCAGGTAAACCCGGACATTCGTATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCAtt                                                                <  1:95721/251‑1 (MQ=255)                                                                                                                                                                           cAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCt                                                              <  1:8212/251‑1 (MQ=255)                                                                                                                                                                                gATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGa                                                         <  1:244500/251‑1 (MQ=255)                                                                                                                                                                                        tAAACCCGGACATTCTGATGCGTATGTCTGCACAGATGGCGCGTCGTCTGCAAGTCCTTTCAGCGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGAACGGACTCTGCTGACTCTGACAAAACAACCAGACGCTATGACTCACCCGGACGGGATGCAAATCTAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGCAGAATTCTTAAGATGCtcga                                                  >  2:478523/1‑247 (MQ=255)                                                                                                                                                                                           accaggACAGTCTGATGCGTTTGTCTGCACAGATGTCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTGCCTCGTCGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACATCCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGAt                                              <  1:32244/247‑1 (MQ=255)                                                                                                                                                                                           aCCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGa                                                  >  1:388885/1‑247 (MQ=255)                                                                                                                                                                                            cccGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGa                                                  >  1:383984/1‑246 (MQ=255)                                                                                                                                                                                             ccGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCa                                            <  2:193757/251‑1 (MQ=255)                                                                                                                                                                                                                tGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTGCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCacac                         >  1:540755/1‑251 (MQ=255)                                                                                                                                                                                                                tGTCTGCACAGATGGCGCGGCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCGCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGcaca                          <  1:584791/250‑1 (MQ=255)                                                                                                                                                                                                                       acaGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGCACCTGATCTCCGCACACGGTaaa                   >  2:33147/1‑250 (MQ=255)                                                                                                                                                                                                                        caGATGGCGCGGCGTCTGAAAGTCATTTCAGAGAAAGTGGGCAAACGGGCGGTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGGCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAAc                 <  2:383984/251‑1 (MQ=255)                                                                                                                                                                                                                                    cgtcTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTc                                                                                              <  2:37197/162‑1 (MQ=255)                                                                                                                                                                                                                                    cgtcTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTc                                                                                              >  1:37197/1‑162 (MQ=255)                                                                                                                                                                                                                                       cTGCAAGTCATTTAAGAGAAAGAGGGCAACCTGGCGTTCCTCGACGTGACGGGACGCATTGAACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGGGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGGAAAACCATAGTCGTCTACgg  >  2:335569/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 CAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCT‑GTTTGAAGAGGGCCAGGAA‑CGTAG‑CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGG  >  NC_000913/3486265‑3486742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑