Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,481,131	G→A	L11L (CTG→TTG)	kefG ←	glutathione‑regulated potassium‑efflux system ancillary protein KefG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,481,131	0	G	A	97.7%	132.0 / NA	45	L11L (CTG→TTG)	kefG	glutathione‑regulated potassium‑efflux system ancillary protein KefG
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (21/22);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCTGGGCAGA  >  NC_000913/3480906‑3481373                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   ggTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGAc                                                                                                                                                                                                                           <  1:279105/251‑1 (MQ=255) ggTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGAc                                                                                                                                                                                                                           <  1:511984/251‑1 (MQ=255)    ccagccacgtgttCAGTAGCGCCTGGCAGCTATAGGTATAAAGAGGATGTAGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCTACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGATGAGAcatc                                                                                                                                                                                                                        <  1:31228/240‑1 (MQ=255)                tCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGAAGCTGAAATAAAAGTACCTCGTGCTCGCCCTGTAATGCCTGCTCACGGTGGATATCAATAAAAAAAGCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACAGTGCTGAGCTGCGTTGGCGGTTTAAGCAGTACCCGGGTTGCCACCGAGTCCTGAGATTCCGGATGGGTATACAACAGCAAAACTATCTCTGGCTGAGACATCATCCCCCTccc                                                                                                                                                                                                             <  2:654672/250‑1 (MQ=255)                  aGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAACGACAATCACCTCGTACTCGCGCAGGAATGCATGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTACGCGTCAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCCTACAACAGCAAAACTTTCGCTGGCTGAGACATCCTCCACCTCCCgg                                                                                                                                                                                                           >  2:427756/1‑250 (MQ=255)                     cgcgcCGGGCAGCTAGAGGTATAAATAGGATGCTGAAAGACAATCACCTCGTTCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATg                                                                                                                                                                                                       <  2:571134/250‑1 (MQ=255)                         ccGGGCAGCTATAGGTAGAAAGTTGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGGAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGttt                                                                                                                                                                                                   <  2:343063/251‑1 (MQ=255)                                         ataAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTg                                                                                                                                                                                   >  1:429615/1‑251 (MQ=255)                                           aaaGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGtt                                                                                                                                                                                 >  1:236442/1‑251 (MQ=255)                                            aaGAGGAGTCTGGAAGACCATCACCTCGTGCTCGCGTAATAATGCCTGCTCACGGGGGATATCAATAAATAAATCGGGATAGGGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGTCGGTTTAATCAGTACCCGGTTTGACTCCGAGTCCGGAGATTCCGGATGGTCATCCAACAGCAATTCTTTCGCTGGCTGTGACATCATCCCCCGCCCGGTATGGTTTTTGTCTATAGTCGCTggtt                                                                                                                                                                                <  2:17832/251‑4 (MQ=255)                                                        aaaGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCAtt                                                                                                                                                                    >  1:219590/1‑251 (MQ=255)                                                        aaaGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCAtt                                                                                                                                                                    >  1:551357/1‑251 (MQ=255)                                                                           cgagctaGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTc                                                                                                                                                 <  2:272247/245‑1 (MQ=255)                                                                                 aGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTc                                                                                                                                                 >  1:272247/1‑245 (MQ=255)                                                                                   tAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCAtac                                                                                                                                         >  1:218852/1‑251 (MQ=255)                                                                                       gCCTGCTCACGGGTGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCCTTGCGCCCGGTGCGGCGGCTCGCCCAtccatt                                                                                                                                      >  2:8427/1‑250 (MQ=255)                                                                                        ccTGCTAACGGGGGATATCAATAAAAAAATCGGGATAGTGATCGTAAAGGTCGTGCACGGTAAAATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGACACCGCGTCATGAGATTCCGGATGGGCATACAACCGCAAACCTTTCGCTGGCTGAGACATCATCCCCCACCCGGTATGGATTTTGTGTATAGTCGCTGTTTTGGTCTACCATTGCGCCCGGTGCGGCAGCTCGCCCAtaacatac                                                                                                                                    >  2:672532/1‑245 (MQ=255)                                                                                            cTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATacattacatt                                                                                                                                 >  1:199506/1‑250 (MQ=255)                                                                                              cACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCGGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTAt                                                                                                                               <  2:219590/250‑1 (MQ=255)                                                                                               aCGGGGGATATCAATAAAAAAATCGGGATAAAGCGCGTAAAGGTCGTGCACGGTAACATTGCAGAGCTGCGTGGCAGGTTTAAGCAGTACCCGTTTTGCCACCGAGTCCTGAGATTCCGGATGGACATACAACAGCAAAACTTTCGCTGACTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATc                                                                                                                              >  1:388894/1‑250 (MQ=255)                                                                                                   gggATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGGGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAAt                                                                                                                         <  2:217434/251‑1 (MQ=255)                                                                                                     gATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGCAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATg                                                                                                                        >  1:70665/1‑250 (MQ=255)                                                                                                      atatCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGAt                                                                                                                      >  1:537650/1‑251 (MQ=255)                                                                                                            aTAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACCTTATCATAATGATAAGtt                                                                                                                 >  1:606345/1‑250 (MQ=255)                                                                                                                 aaaaTCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATa                                                                                                           <  1:414148/251‑1 (MQ=255)                                                                                                                  aaaTCTGGATATTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCGTCGTGGCCGGTTTAAGCAGTACCCGGTGTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAATCTTTCGCTGGCTGATACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAg                                                                                                          <  1:431328/251‑1 (MQ=255)                                                                                                                    aTCGGGTTAGTGGGCGTTAAGGTAGTGCACGTTAACATTGCTGAGCCGCGGGGCCGGTTGAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATGCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGGATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTATAATAGTc                                                                                                        <  2:534651/251‑1 (MQ=255)                                                                                                                    aTCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTc                                                                                                        >  2:156524/1‑251 (MQ=255)                                                                                                                      cGGGATAGGTCGCGTAAAGGTCGGGCACGGTAACATTTCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATTCAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGTCCGGTGCGGCAGCTCGCCCATAAATTACATTTTCATAATGATAAGTTAACATAGTCt                                                                                                       <  2:389241/250‑1 (MQ=255)                                                                                                                      cGGGAGAGGGCGCGTAAAGGTCGGTCCCGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGGTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATGATCATAATGATAAGTTAACATAGTCTg                                                                                                      <  2:316699/251‑1 (MQ=255)                                                                                                                           tAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACAt                                                                                                 >  1:408190/1‑251 (MQ=255)                                                                                                                               gcgcGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCTCTGTTTTGGGCTACCATTGCGCCCGGTGCGGTAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACgg                                                                                             <  1:316246/251‑1 (MQ=255)                                                                                                                                               cACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGttt                                                                             <  1:484956/251‑1 (MQ=255)                                                                                                                                                ccGGTAACAATGCCGAGCTGCGTGGCCGGTTTAAGCAGTCCCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGATGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTggtt                                                                             <  2:575604/249‑4 (MQ=255)                                                                                                                                                      actgtGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTCTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGTGACATCATCCCCCTCTCGGTATGGTTTTTGTTTATAGTCGCTGTTTTGGGCTACCATTGCGCCCTGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACTGCACCTTATTATTGTTTTctcctc                                                                      <  1:387394/247‑1 (MQ=255)                                                                                                                                                        aTTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGt                                                                    >  1:612824/1‑251 (MQ=255)                                                                                                                                                               aGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGAt                                                                                  >  2:500442/1‑230 (MQ=255)                                                                                                                                                               aGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGAt                                                                                  <  1:500442/230‑1 (MQ=255)                                                                                                                                                                    cGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATtcgtc                                                        <  1:552342/251‑1 (MQ=255)                                                                                                                                                                    cGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATtcgtc                                                        >  1:168855/1‑251 (MQ=255)                                                                                                                                                                    cGTGGCCGGTTTAAACAGTAACCGGTTTACCACCGAGTCCTGAGATTCCGGATGGGCATACACCAGCACAACTTTCGCTGGCTGAGACATCATCACCCTCCCGGTCTGGTTTTTGTGTATTGTCGCTGTTTTGGGCTACCATTGCGCCCGGAGCGGCAGCTCGCCCATACATTACATTCTCCTAATGCTAAGTTAACATCGTCTGAACATACGGCAACTTATGATTGTTTTCTCATCGTTACAACTtcatc                                                        >  2:66435/1‑248 (MQ=255)                                                                                                                                                                                aaGCAGTACCCGGATTGCCAACGAGTCCTGAGATTACGTATGGGCATCCAGCAGCAAAACCTTCTATGGCTTATACATCATCCCCCTCCTGGTATGGTTTTTGTCTATAGTTGTTGTTTTTGGCTACCATTGCGCCCGGTGCTTCAGCTCGCCCATACATTTCATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTACGATTGTTTGCTCCTCGTTACAAATCCTTCGCGGCGTgcgct                                            <  2:72856/251‑2 (MQ=255)                                                                                                                                                                                                    ccGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATgccacc                        >  1:135216/1‑251 (MQ=255)                                                                                                                                                                                                        gTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATgccaccgcc                     >  1:247446/1‑250 (MQ=255)                                                                                                                                                                                                                          ggaaTACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATTATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCGCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCTGGGCAGa  <  1:285194/248‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   GGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCTGGGCAGA  >  NC_000913/3480906‑3481373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑