Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,433,900	C→T	L71L (CTA→TTA)	def →	peptide deformylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,433,900	0	C	T	96.3%	155.7 / NA	56	L71L (CTA→TTA)	def	peptide deformylase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (27/25);  minor base G (1/1);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGCTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTATCTGTCACCGCTG  >  NC_000913/3433654‑3434139                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                aatCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAgt                                                                                                                                                                                                                                             <  1:93358/251‑1 (MQ=255) aatCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAgt                                                                                                                                                                                                                                             <  1:308671/251‑1 (MQ=255)           attccagAACACATCTCGGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGAATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTAGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGCCGAACTGTTAGGGTTaatcaat                                                                                                                                                                                                                                   >  2:616425/7‑251 (MQ=255)                 gAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGCCGAGCGGCTTCGCAAAGTTGCTAAACAGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCag                                                                                                                                                                                                                               >  1:625658/1‑249 (MQ=255)                         ctctGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAAACGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATCTGATCGAGACGATGTACGCAGAAGAAGGTATTGGCAGGGCGGCAACCCAGGTTGGAATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATAAATCCCGAGCtttt                                                                                                                                                                                                                        >  1:638452/1‑248 (MQ=255)                           ctGGAGATTTAAGTCAGTTTTGCAAATGATACATATTCCTGAAGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGCAGTGAATGCAGAAATTCAGCGTATCGTCGGTGATATGTTCGAGACGTTGTCCGCAGAAGAAGCTATTGGCCTGGCGGCAACAACGGAAGATCTCCATCAACATATCATTGTTATTGATGTTTCGGAACCACGTGACGAAAGGGTAGAGTTAATCACTCCAGAGCTTTTAGaa                                                                                                                                                                                                                    >  2:397511/1‑250 (MQ=255)                             ggAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTATTGTTAATCAATCCAAAGCTTTTAGaaaa                                                                                                                                                                                                                  >  1:320750/1‑250 (MQ=255)                                        tCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAAc                                                                                                                                                                                                      >  1:303428/1‑251 (MQ=255)                                            ttttGCAAGTGATACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGCATGCAGAAATTCAGCGGATCGTCGATGATATGTTCGAGTCGCTGTACGCAGAAGAAGGTATTGGCCTGGAGGCAACCCAGGTTGATCTCCATCAACGTATCATTGTTATTGATGTTTCGGAAACCCGTGACGAACGGCTAGTGTTAATAGCTCCAGAGCTTTTAAAAAAACGCGGCGAAACAGGc                                                                                                                                                                                                  >  2:108147/1‑251 (MQ=255)                                                gCAAGTGTTACATATTCCGGACGAGCTGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGGGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTTATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCCTTTAGAAAAAAGCGGCGAAACAGGCAttt                                                                                                                                                                                              <  2:473411/251‑2 (MQ=255)                                                      gtTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGaagaag                                                                                                                                                                                        <  1:283589/251‑1 (MQ=255)                                                              ttCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTg                                                                                                                                                                                >  1:414636/1‑251 (MQ=255)                                                                      gAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGCAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGAAccc                                                                                                                                                                        >  1:483065/1‑251 (MQ=255)                                                                          ggCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGATGATATCCCTCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTCGTGTTTATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGAATTGAAGAAGGTTGCCTGTCGATCCCTGa                                                                                                                                                                     >  2:204044/1‑250 (MQ=255)                                                                                gCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGt                                                                                                                                                              >  1:240004/1‑251 (MQ=255)                                                                                     gTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCttt                                                                                                                                                         >  1:505520/1‑251 (MQ=255)                                                                                              ccGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCcgcg                                                                                                                                                >  1:451794/1‑251 (MQ=255)                                                                                                    gaagaaGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCaga                                                                                                                                          <  1:95793/251‑1 (MQ=255)                                                                                                     aagacgTGAATTCATAAATTCATGGTATCTTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCCACCCAGTTTGATATCCTTCAACGTATCATTGTTATTGATGTTTCGGAAAACCGGTACGAGCGGTTAGTGTTAAGCAATCCAGAGCTTTTAGAAAAAAGCGGCGAATCAGGCATTTAAGCAGGTTGCCTGTCGATCCCTGAACATCGTGCTTTAGTGCCGCGCGCagag                                                                                                                                         <  2:173663/251‑1 (MQ=255)                                                                                                     aagaagTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCag                                                                                                                                           >  1:307938/1‑249 (MQ=255)                                                                                                        aagTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGAATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGCAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGATTTAGAGCCGCGCGCAGAGaa                                                                                                                                       >  1:73675/1‑250 (MQ=255)                                                                                                                   gAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTcg                                                                                                                           >  1:574287/1‑251 (MQ=255)                                                                                                                      aTTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAATGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTACCGCGCGCAGAGAAAGTTAACATTCgcgc                                                                                                                        >  1:121244/1‑251 (MQ=255)                                                                                                                                             gTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAATCCAt                                                                                                 <  2:481408/251‑1 (MQ=255)                                                                                                                                                cGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCAttt                                                                                               <  1:88040/250‑1 (MQ=255)                                                                                                                                                 gagaCGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCa                                                                                                  >  1:59/1‑246 (MQ=255)                                                                                                                                                 gagaCGATGTACGCAGAAGAAGGTATTGGCCGTGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGGTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCTCTTGACCGCGACGGTAAACCa                                                                                                  <  2:59/246‑1 (MQ=255)                                                                                                                                                    aCGATGTACGCAGAAGAAGGTATTGGCCTGGCGGTAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCGTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACt                                                                                          <  2:121244/251‑1 (MQ=255)                                                                                                                                                    aCGATGTACGCAGAAGAAGGTATTGGCCTGGAGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGACACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGCGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACt                                                                                          >  2:666612/1‑251 (MQ=255)                                                                                                                                                        tGTACGCAGAAGAAGGAATTGGCCTGGCGGCAACCCAGGTTGTTATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGATAGTGTTAATCACTCCAGAGCTTTTAGAAAAAAGCGGCGTAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACCACGAGCTTTAGTGCCGCGCGCAGGGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGAAACCCATTTGacgtgga                                                                                       >  2:124229/1‑244 (MQ=255)                                                                                                                                                         gTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGaa                                                                                      <  1:666612/250‑1 (MQ=255)                                                                                                                                                           aCGCAGAAGAAGGTATTGTCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCa                                                                                   <  1:368839/251‑1 (MQ=255)                                                                                                                                                                        tATTGGCCTGGCCGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGAGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAAACATTTGAACTGGAAGCAGACGGTCTGtt                                                                        >  1:473837/1‑249 (MQ=255)                                                                                                                                                                        tATTGGCCTGAAGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCAAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGGACAAAGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGAAACCCATTTGAACTGGAAGCAGCCGGGCTGTTa                                                                       >  2:435034/1‑250 (MQ=255)                                                                                                                                                                           tGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGcc                                                                    <  1:204044/250‑1 (MQ=255)                                                                                                                                                                            ggCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCGTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCAt                                                                  <  1:43503/251‑1 (MQ=255)                                                                                                                                                                            ggCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGGTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTGGAACTGGAAGCAGACGGTCTGTTAGCCa                                                                   <  1:152354/250‑1 (MQ=255)                                                                                                                                                                              ccTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTcgc                                                                                                                          >  1:6508/1‑193 (MQ=255)                                                                                                                                                                              ccTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCAGTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGGGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTcgc                                                                                                                          <  2:6508/193‑1 (MQ=255)                                                                                                                                                                                              ggtggtatCCATCAACGTAACATTGTGATTGAATTTGCGGAAAACCGTGACGAACGTGTAGTGGTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGGCGATCCCTGAACAACGTGCTTTAGTTCCGCGCTCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTAGTCAGCATGAGAt                                                <  2:564697/246‑1 (MQ=255)                                                                                                                                                                                                tGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGACCAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATgg                                              >  2:369517/1‑251 (MQ=255)                                                                                                                                                                                                 gATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTgg                                                                                        >  1:585960/1‑208 (MQ=255)                                                                                                                                                                                                 gATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCACTTGACCGCGACGGTAAACCATTTGAACTgg                                                                                        <  2:585960/208‑1 (MQ=255)                                                                                                                                                                                                 gATATCCATCAACGTATCATTGTTAATGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATgg                                              <  2:233249/250‑1 (MQ=255)                                                                                                                                                                                                    atCCATCAACGTATCATTGTTATTGACGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATAAGATGGATc                                           <  2:451794/250‑1 (MQ=255)                                                                                                                                                                                                      ccATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCAc                                         <  2:295565/250‑1 (MQ=255)                                                                                                                                                                                                       cATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCt                                       <  1:397511/251‑1 (MQ=255)                                                                                                                                                                                                          cAACGTATCATTGTTATTGATGTTTCGGCAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCAGCGCGCAGAGAAAGTTAAACTTCGCGCCCTTGACCGCGATGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTCGCCATCTGTATTAAGCATGAGTTGGATCGCCTGGt                                    >  2:162432/1‑251 (MQ=255)                                                                                                                                                                                                           aaCGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTc                                   >  1:8895/1‑251 (MQ=255)                                                                                                                                                                                                                attatttttATTGAGGTTGTGGAAAACCGTGACGACCGGTTAGTGTTAATCAAGCCAGACCTTTATGAAAAAAGAGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACCACGTGCTTTAGTGCCGCGCGCAGAGAACGTTAAAATTCGCGCTCTTGACCTCGACGTTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTTAGCATTAGATGGATTTCCTGGTCGGCa                               <  2:73675/243‑1 (MQ=255)                                                                                                                                                                                                                 tCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACAATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCaa                              >  1:224786/1‑250 (MQ=255)                                                                                                                                                                                                                  cATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCaaa                             <  1:431764/250‑1 (MQ=255)                                                                                                                                                                                                                        tATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGttt                       <  1:311329/250‑1 (MQ=255)                                                                                                                                                                                                                                         aCCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTATCTGTCACCg     <  1:479318/251‑1 (MQ=255)                                                                                                                                                                                                                                           cGTGACGAACGGTTAGAGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTAGTGATTTTCTGTCACCGCt   >  2:15393/1‑251 (MQ=255)                                                                                                                                                                                                                                            gTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGCGAAAGTTAAAATTCGCGCCCTTGACCGCGGCGGGAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTATCTGTCACCGCTg  >  1:389825/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                AATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGCTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTATCTGTCACCGCTG  >  NC_000913/3433654‑3434139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑