Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,199	T→A	R133R (CGT→CGA)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,199	0	T	A	97.1%	193.9 / ‑3.3	68	R133R (CGT→CGA)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (37/29);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.22e-01

TTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTT  >  NC_000913/2955‑3429                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       cacgctcgcccgatccttcaaaaCCACGGGTAAATAGCGTTTATCAGTGCGGGTAGCGTTTTTGCCAGGAGCTTGGCAATCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATTACACGCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTcc                                                                                                                                                                                                                                  <  2:378012/230‑1 (MQ=255)     cGATAAGCTGCCGTCAGATCCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGgca                                                                                                                                                                                                                              <  1:475998/251‑1 (MQ=255)        tAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGACAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGGTTAATGCCACTCGTTTTCTGGCTTTTATGGGCCGA‑CTGACAGGACGAATCTCCGgcagcc                                                                                                                                                                                                                           >  2:452553/1‑250 (MQ=255)          aGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGcatt                                                                                                                                                                                                                         >  1:287581/1‑251 (MQ=255)                 gTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAAtctca                                                                                                                                                                                                                                  >  1:378012/1‑234 (MQ=255)                 gTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACg                                                                                                                                                                                                                  >  1:46403/1‑251 (MQ=255)                 gTCAGAACCACGGGAAAATATCGTTTAGCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAAGCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCGGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACg                                                                                                                                                                                                                  <  1:618009/251‑1 (MQ=255)                        ccACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGt                                                                                                                                                                                                           >  1:65022/1‑251 (MQ=255)                        ccACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGt                                                                                                                                                                                                           <  2:636959/251‑1 (MQ=255)                          aCGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATTGGC‑GAGCTGGAAGGACGAATCTCCAGCAGCATTCATTACGACAACGTgg                                                                                                                                                                                                         <  1:501287/251‑1 (MQ=255)                            gggAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCATGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCa                                                                                                                                                                                                       <  1:342319/251‑1 (MQ=255)                                 aaTATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCgtg                                                                                                                                                                                                  <  1:137465/251‑1 (MQ=255)                                   tatCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCCAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCCATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGGACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGCAGGACGAATCTCCGGCAGCCTTCCTTACGACAACGTGGCACCGGGtt                                                                                                                                                                                                >  2:381404/1‑251 (MQ=255)                                                 gcggggAGCGTTTGTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATg                                                                                                                                                                                  <  2:287581/248‑1 (MQ=255)                                                 gCTGGGATCGTTTTTGCCCGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTTGAAAAGAATATGCCAATCGGTTCGGGCGTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGTGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATg                                                                                                                                                                                  <  2:21446/251‑1 (MQ=255)                                                     ggaggttttgtgcaaggagcttggTAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGATTTAGGCTCCAGCTCCTGTTCGGTGGTCGCGGCTCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGt                                                                                                                                                                              <  2:486787/229‑1 (MQ=255)                                                          gTTTTTGACAGGAACTTGGCAAGCAAATTCCCGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGGTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGAAGAATCTCCGGCAGCATTCATTACGACAGCGTGACACCTGGTTTTCTTGGTGGAATGCAGTtgatg                                                                                                                                                                         >  2:615022/1‑251 (MQ=255)                                                            ttttGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTtgatga                                                                                                                                                                        <  1:591318/250‑1 (MQ=255)                                                                 ccAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGATGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCga                                                                                                                                                                    >  1:434613/1‑249 (MQ=255)                                                                 ccAGGAACTTGGAAAGCAACTTCCAGTGGCGATGACTCTGGTAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGCATGAACAATGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTTGCAGGACGAATCTCCGGCAGCATTCATTACGCCAACGTGGCACCTTGTTTTCTTGGAGGTATGCAGTGTATGATCga                                                                                                                                                                    >  2:248952/1‑249 (MQ=255)                                                                             caaGCAAATGCCAGTGGCGATGACTCTGGAAAATAATATGCCAAGCGGTTCGGGCTTAGGCTCCAGCGCCTGTGCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCGTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGAcatca                                                                                                                                                      <  2:453374/250‑1 (MQ=255)                                                                              aaGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTGGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGCTGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGAcatcat                                                                                                                                                     >  1:566781/1‑251 (MQ=255)                                                                                gCAAATTCCAGTGGCGATGACTCTGGAGAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGCACACTGTGGCCAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGACTCTCCGGCAGCAGTCATTAGGACTACGTGGCACCGTGTTTTCTTGGAGGAATGCAGTTGATGATCGAAGAAAACGAcgacatc                                                                                                                                                    >  2:387716/1‑244 (MQ=255)                                                                                         aGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCa                                                                                                                                            >  1:209199/1‑249 (MQ=255)                                                                                           tGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGCCGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGGGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCaaagg                                                                                                                                        >  2:137276/1‑248 (MQ=255)                                                                                           tGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCaa                                                                                                                                           <  1:137276/248‑1 (MQ=255)                                                                                            ggCGATGACTCTGGAAAAGACTATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGc                                                                                                                                       >  1:487224/1‑251 (MQ=255)                                                                                            ggCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGc                                                                                                                                       <  1:449166/251‑1 (MQ=255)                                                                                               gATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGAGCTGATGGCGATGAATGAAAACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GCGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAAAGTGGCACCGAGTTTTATTGGTGGTATGCAGTTGATGATCGAAGAAAAAGACATCATCAGCCAGAAAGTGcc                                                                                                                                      >  2:496142/1‑249 (MQ=255)                                                                                                        ggAAAAGAATATGCCAATCGGGTCGGGCTTAGGCTCCAGCGGCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTtga                                                                                                                             <  2:321868/249‑1 (MQ=255)                                                                                                         gAAAAGAATGTGCCAATCGGTTCGGGCTTAGGCCCCAGCGCTTTGTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACGCTGTGGCAAGCCTCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GTGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCTAGTGCCAGGGTTtgatga                                                                                                                          <  2:434613/251‑1 (MQ=255)                                                                                                                   tGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTgg                                                                                                                >  1:611675/1‑251 (MQ=255)                                                                                                                     ccactCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTgggg                                                                                                              >  2:199385/5‑250 (MQ=255)                                                                                                                             ttCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGgcag                                                                                                                                                                                                                             <  1:127663/132‑1 (MQ=255)                                                                                                                             ttCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGgcag                                                                                                                                                                                                                             >  2:127663/1‑132 (MQ=255)                                                                                                                               cGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTAt                                                                                                    >  1:292297/1‑251 (MQ=255)                                                                                                                               cGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGAC‑GAGCTGGAAGGACGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGAGGCTGTGGGTGCTGGCGTAt                                                                                                    >  1:117678/1‑251 (MQ=255)                                                                                                                                 ggCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATc                                                                                                                                                                      >  2:121763/1‑183 (MQ=255)                                                                                                                                 ggCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATc                                                                                                                                                                      <  1:121763/183‑1 (MQ=255)                                                                                                                                   cTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCgg                                                                                                >  1:536996/1‑251 (MQ=255)                                                                                                                                      aGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCgggg                                                                                              <  1:381404/250‑1 (MQ=255)                                                                                                                                          tCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGGGTGGCTGTGGGTGCTGGCGTATCCGGGGATTa                                                                                          >  1:281530/1‑250 (MQ=255)                                                                                                                                                gCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGtctc                                                                                   <  1:70200/251‑1 (MQ=255)                                                                                                                                                    gTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGAGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACg                                                                               >  1:100356/1‑251 (MQ=255)                                                                                                                                                         gtggtCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGGTTAAAGTCTCGACGGc                                                                             >  2:139006/1‑248 (MQ=255)                                                                                                                                                         gggggcgcgGCGCTGAGGGCGAATAAAGAACTCTGTGGCAAGCCGCTGAAGGACACGCGTTTGCTGGCTTTGATGGGC‑GTGCTGGAAGGACTAATCTCCGGCAGCAGTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGa                                                                          <  2:566781/246‑1 (MQ=255)                                                                                                                                                           ggtCGCGGCGCTGAGGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGTAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGaa                                                                         <  1:396453/250‑1 (MQ=255)                                                                                                                                                            acccgtcgctcgtccgcccttaatgaaCACTGGGGCAAGCCGCTGAAGGACACTCGTTTGCTGGCTTGGATTGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATGCATTACGACAACGGGGCTCCGGGTTTTCGTGGTGGTATGCAGGTGAGGATCGAGGAAAACGACATCATCAGCCAGCAAGGGCCAGGGTTGGATGAGTGGATGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGc                                                                       <  2:498031/230‑1 (MQ=255)                                                                                                                                                              cgcgGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCa                                                                     <  1:615022/251‑1 (MQ=255)                                                                                                                                                                cggcgCTGATGGCGCTGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGGTGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAACGTCTCGACGGTAGacaccagg                                                                   >  2:633784/1‑244 (MQ=255)                                                                                                                                                                   cgcTGATGGCAACGAATGAACCCTGAGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGCTGGGC‑GAGCAGGAAGGACGAATCTCCGGCAGCATTCATTACGACAGCGTGGCACCGTGTGTTCTTGGCGGGATGAGGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGGGCAAGGGTTTGATGAGTGGCTGGGGGTGCTGGAGTATCCAGGGATTAACGTCGCAACGGCAGACGCCGGGGCt                                                                >  2:439399/1‑251 (MQ=255)                                                                                                                                                                      tGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTAtt                                                             >  1:237184/1‑251 (MQ=255)                                                                                                                                                                       gagggCGATGAATGAACACTGTTGCAAGCCGCTTAATGACACCTGTTTTCGGTCTTTGATGGGC‑GAGCGTGAAGGACGAATCTCCGGCAGCATTCATTCCGACAACGTTGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTAt                                                              <  2:46403/246‑1 (MQ=255)                                                                                                                                                                        atgtcGATGAATGAACACTGTGGCAAGCCGCTTAAGGACACTCGTTTGCTGGCTTTTATGGGC‑GAGATGGAAGGACGAATCTCCGGCAGCATTCATTACTACAACGTGGCAACGTGTTTTCTTGGTGTTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTAgttt                                                           <  2:100356/247‑1 (MQ=255)                                                                                                                                                                                gaatgaaCACTGTGGCAAGCCGCTTAATGACCCTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGc                                                                       >  1:498031/1‑231 (MQ=255)                                                                                                                                                                                        acTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATcgc                                           >  1:557738/1‑251 (MQ=255)                                                                                                                                                                                                   ccGCTTAATGACCCTCGGTTGCTGGCTTTGATGGGC‑TAGCTGGAAGGACGATTCTCCGGCAGTAGTCATTACGACAACGTGGCACCGTGTTGTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGACAGGattg                                <  2:487224/251‑1 (MQ=255)                                                                                                                                                                                                         aaTGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGattgcattgc                          >  1:345240/1‑251 (MQ=255)                                                                                                                                                                                                          aTGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTgcg                         >  1:580161/1‑251 (MQ=255)                                                                                                                                                                                                            gACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCAAGGCTTGCATTgcgc                        >  2:259432/1‑250 (MQ=255)                                                                                                                                                                                                               acTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTCTCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTTCGCACgg                    >  2:382460/1‑251 (MQ=255)                                                                                                                                                                                                                        cTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTAATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCt           >  1:573873/1‑251 (MQ=255)                                                                                                                                                                                                                        cTGGCTTTGAAGGTC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTAGGAGAACGAGGCACCGTGTTTTCTTGGTGGTATGCAGTTGGTGATCGCAGACAACGACATCATCAGCCAGCGAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGAGTCCGGGGAATAACGTCTCGACGGCAGAGGCCAGGGCTATTTTACCGGCGTAGTATCGACGCCAGGAATGCAGTGCGCACGGGCGGCATCt           >  2:330807/1‑251 (MQ=255)                                                                                                                                                                                                                          ggCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCAGTACGACAACGTGGCACCGTGTGTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCATGGCTAGTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCt           <  1:34332/249‑1 (MQ=255)                                                                                                                                                                                                                            cattGATGGGC‑GAGCTGGAAGGACGAATATCCGGAAGCATTCATTACGACAACGTGGCACCGTGGTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGATTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGAATCGCCGCCAGGATTGCATTGCGAACGGGCGACATCTGGct       >  1:170117/3‑250 (MQ=255)                                                                                                                                                                                                                                gATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACATCGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGct       <  1:343255/247‑2 (MQ=255)                                                                                                                                                                                                                                gATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCCCGACGGCAGCAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGACTGCATTGCCCACGGGCCATATCTGGcttgat   >  2:343255/1‑246 (MQ=255)                                                                                                                                                                                                                                 aTGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCtt  <  1:259432/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       TTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTT  >  NC_000913/2955‑3429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑