Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,433,665	C→T	intergenic (‑105/‑25)	smf ← / → def	protein Smf/peptide deformylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,433,665	0	C	T	100.0%	144.0 / NA	46	intergenic (‑105/‑25)	smf/def	protein Smf/peptide deformylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/22);  total (24/22)

AAGCCCTGTTTGCTGCAATA‑CAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGCTAGT  >  NC_000913/3433429‑3433904                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                atgCCCTGTTTGCT‑CAATAACACCCGCATCATTTTGCGACTGTGTTGCCACCAATGGAGCGATACGGACATTATCATCGCCGTAAAAGCTGCGGATCCTCATTAAACGCAGCCAAATTTCTGTATCGCCCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGTGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACAtct                                                                                                                                                                                                                                   <  2:218986/249‑1 (MQ=255)        gTTTGCTGCAATA‑CAACCGCATCAATATGAGTCTGGTTTGCCGCCCAGTGATCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCCCCCTGCCATAATCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGgag                                                                                                                                                                                                                             <  1:198459/250‑1 (MQ=255)                    a‑CAACCGCACCAATATGCGACTTTTTTGCCACCCAGTGAGCAATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGt                                                                                                                                                                                                                 <  2:145518/250‑1 (MQ=255)                          tgCATCAATATGCGACTGTTTTGCCACCAAGTGAGCGATACGGACCATATCATCGCAGTACAAGATGCTGATACTCATTAAACGCAGCCAACTTTCTGTATCGACCATCCTTATCTCCCTGCCATAGACAGCCTTCAAACTCTTTGCG‑TTAGTCCAGTGTGGCTGTCAATCGGGGGGGGATTTGTCTAGAATAGAAGAAGTAATATTTCTATTTCCTGAACACATCTTTGGGGTTTTATTTCAGtttaga                                                                                                                                                                                                            >  2:43503/2‑247 (MQ=255)                                aaTATGCGACTGTTTTGCCACCCAGTGAGCGATACTGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCATTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAACTAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGtt                                                                                                                                                                                                     >  1:586503/1‑251 (MQ=255)                                   ttGCGACTGGTTTGCCGCCAAGTGAGCGATTCGGACCATATCACCGCCGTCAAATCTGCTGCTCCCCAGTAAACGCAGCCAAATTTCTGTATCTACCAGCCTTACCTCCCTGCCATAAGCAGCCGTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGATGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTAc                                                                                                                                                                                                   <  2:382684/249‑1 (MQ=255)                                   atGCGACTGTTTTGCCCCCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACa                                                                                                                                                                                                  <  1:491392/251‑1 (MQ=255)                                      cGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACatat                                                                                                                                                                                               >  1:484305/1‑251 (MQ=255)                                                 ccaaccAGTAAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAATGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCt                                                                                                                                                                                                                                             >  2:448886/5‑194 (MQ=255)                                                 ccACCCAGTGAGCGATCCGGACCATATCATCTCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCt                                                                                                                                                                                                                                             <  1:448886/194‑1 (MQ=255)                                                  cACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCg                                                                                                                                                                                   >  1:236078/1‑251 (MQ=255)                                                        gTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCg                                                                                                                                                                             <  1:508510/251‑1 (MQ=255)                                                                   gaaCCATATCATCGCCGGACAAGCTGCTGTTTCTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTAATCCCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCGGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTGTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATACCGGACGAGCGGCTTCGCAAAGTTGCTa                                                                                                                                                                  <  2:676262/249‑1 (MQ=255)                                                                      ccATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCACAGtttataaac                                                                                                                                                               >  2:311329/1‑244 (MQ=255)                                                                        atatCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCg                                                                                                                                                             >  1:564697/1‑251 (MQ=255)                                                                                    tACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTg                                                                                                                                                 >  1:358638/1‑251 (MQ=255)                                                                                     aaaaGCTGCTGATACTCATTAAACGCAGACAACTTTCTGAATCGAACATACTTCTCTCACTGACATAAGCAGCATTAGCAATCTTTGCGATTGGTC‑AGAGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGTAGAAATAATCTTTCTAATTCCTGAACACATCTCTGAAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGATCGGATTAGCAAAGTTGATAAACAGGATGAAGAAGTg                                                                                                                                                 >  1:170011/3‑250 (MQ=255)                                                                                          ctgctgATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTGCCTGCAATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTAAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACAtc                                                                                                                                                                                                                                    >  1:403345/1‑162 (MQ=255)                                                                                               gATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAAt                                                                                                                                      <  1:421129/251‑1 (MQ=255)                                                                                                       ttAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGt                                                                                                                                                  <  1:511460/231‑1 (MQ=255)                                                                                                       ttAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGAAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGt                                                                                                                               >  1:173663/1‑250 (MQ=255)                                                                                                       ttAAACGAAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTTCCATAAGCAGCCTTAGCAATATTTGCGATTGGTA‑AGTGATGCTGTCAATCAGAGGGGGATTTGTATAGAATAGAAGAAATAATCTTTCTAATTCATGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGaagaaggagatcgaaagggcgtcgggt                                                                                                                              >  2:511460/1‑230 (MQ=255)                                                                                                        tAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTAt                                                                                                                             >  1:481408/1‑251 (MQ=255)                                                                                                           aCGCAGCCAAATTTCTGTATCGACCATCCTTAACTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTAtcgt                                                                                                                          <  1:258190/251‑1 (MQ=255)                                                                                                                           ttatcgccaccctttacctcCCTGCCATAAGCAGTCTTATCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGGCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGACGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCga                                                                                                           <  2:517022/233‑1 (MQ=255)                                                                                                                             accGACCATCCTTAGCCCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGGC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCC‑GAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGAc                                                                                                        <  1:44318/248‑1 (MQ=255)                                                                                                                                 ccctcctttATCCCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGTCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAAGTCAGCGTATCGTCGATGATATGTTCTAGTCGATg                                                                                                    <  2:229657/244‑1 (MQ=255)                                                                                                                                    aTCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTAc                                                                                                 <  1:506193/251‑1 (MQ=255)                                                                                                                                                           agcCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGACGTACGCAGAAGAAGGTATTGGCCTggc                                                                          >  2:471890/1‑251 (MQ=255)                                                                                                                                                                        tttGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGCTGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAggct                                                             >  1:237216/1‑249 (MQ=255)                                                                                                                                                                                  gTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATc                                                   <  1:556609/251‑1 (MQ=255)                                                                                                                                                                                       gTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACg                                               <  1:471890/251‑1 (MQ=255)                                                                                                                                                                                          aTGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACACAGGTTGATATCCATCGACGTa                                             >  1:324964/1‑250 (MQ=255)                                                                                                                                                                                           ggCTGTCAATCAGAGGGGGCTTTGTCTAGAATAGAAGAAATAATCTTTCTAATGCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGGCGAGCGGCTTCGCAAAGTTGCTAAATCTGTAGAAGAAGTGAATGCAGAAATTCAGCGTAGCGTCGATGATATGTTCGAGACGATGTATGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATc                                           <  1:403440/250‑1 (MQ=255)                                                                                                                                                                                               gTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTg                                       >  2:431764/1‑251 (MQ=255)                                                                                                                                                                                                     cAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTg                                 >  1:295565/1‑251 (MQ=255)                                                                                                                                                                                                     cAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTg                                 >  1:233249/1‑251 (MQ=255)                                                                                                                                                                                                        agGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATg                              <  1:285888/251‑1 (MQ=255)                                                                                                                                                                                                         gggggATTTGTCTAGAATATAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTTCACGTGTTACATATTCCGGACGAGCTGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGATCGAGACGTTGTACGCAAAAGAAGGTATTGGCCTTGCGTCAACCCAGGTTGATATCCATCAACATATCATTGTTATTGAttt                             >  2:152354/1‑249 (MQ=255)                                                                                                                                                                                                               tttGTATAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACTTATTCCGGACGAGCGGCGTCGCACAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATAATATGTTGGAGACGATGTACGCAGAAGAAGGTATTGGCCGGGCGGCAACCCAGGTTGATATCCATCAAAGTATCATTGTTATTGATGTTTCgg                        >  2:134418/1‑250 (MQ=255)                                                                                                                                                                                                                    cTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGt                                                                                     >  1:116868/1‑184 (MQ=255)                                                                                                                                                                                                                    cTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACAGCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGt                                                                                     <  2:116868/184‑1 (MQ=255)                                                                                                                                                                                                                       gaatagaaGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTg               >  1:581409/1‑251 (MQ=255)                                                                                                                                                                                                                            gaagaaATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGAGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATAGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAAcagttccga           >  2:479318/1‑242 (MQ=255)                                                                                                                                                                                                                                    aatCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAgt  <  1:93358/251‑1 (MQ=255)                                                                                                                                                                                                                                    aatCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAgt  <  1:308671/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                AAGCCCTGTTTGCTGCAATA‑CAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC‑AGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGCTAGT  >  NC_000913/3433429‑3433904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑