Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,296,481 G→A V25M (GTG→ATG)  dolP → division and outer membrane stress‑associated lipid‑binding lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,296,4810GA98.0% 311.2 / ‑4.2 101V25M (GTG→ATG) dolPdivision and outer membrane stress‑associated lipid‑binding lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (55/44);  total (57/44)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 101 total aligned reads displayed.

CCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAGTGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACG  >  NC_000913/3296236‑3296724
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
ccGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTTCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAC‑GGTT‑GTGTTGCCGCTGCCGTAATGGGt                                                                                                                                                                                                                                                   >  2:677436/1‑251 (MQ=255)
 cGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTa                                                                                                                                                                                                                                                  <  1:181670/251‑1 (MQ=255)
 cGGCGATGACGGCGGAGAACTTGCATGTTTGTTAGTGCCACAGGATGGGGAGATCCGCATTCCGTCGCATCGTAGTGCTCGCAGTCAGGAAAGGCATATGCTGACTGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATGATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTa                                                                                                                                                                                                                                                  <  2:352255/251‑1 (MQ=255)
  ggtgaTGACGGCGGCGAACTTTCAGGTTTGGTAGGGCCACAGGAGGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAAGTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GGGTTGCCGCTGCCGTAATGGGTAc                                                                                                                                                                                                                                                 <  2:274076/247‑1 (MQ=255)
    cTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCg                                                                                                                                                                                                                                               >  2:675188/1‑251 (MQ=255)
     tATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCGTATGCTGACGGTAAATTGCCGGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAGGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTAGCAA‑GGTT‑GTGTGGCCGCTGCCGTAATGGGTACCgc                                                                                                                                                                                                                                              >  2:146084/1‑251 (MQ=255)
      aTGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCgct                                                                                                                                                                                                                                             >  1:17282/1‑251 (MQ=255)
       tGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGTTGTGGAGATCCGCATGCCTTCGCATCGTAGTGCGCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCTCCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCTCTGCTGTTGCAA‑GGTT‑GTTTTGCCGCTGCCGTAATTGGTACCgct                                                                                                                                                                                                                                             <  2:619814/250‑1 (MQ=255)
           ggcggcGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑tgt                                                                                                                                                                                                                                       >  1:589571/1‑251 (MQ=255)
            gcgggtaatgtGCAGGTTTGTTAGGGCCAAAGGATGTGGAGATCGGCAGTCCTGCGCATCTTAGGTCCCCCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGTTCTGATCGATAACGCGCTTTGCCCCCACCAGGATGATTAAGTAGAATACATGAAGGCATAACCGCCAATCGCAGGCCTTAGTTCTGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCTGTAACGGGTAACgctgc‑tttg                                                                                                                                                                                                                                      <  2:579773/241‑4 (MQ=255)
            gcggcgAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑tgtg                                                                                                                                                                                                                                      <  1:17702/251‑1 (MQ=255)
              ggcgAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTCCCGCTGC‑TGTggg                                                                                                                                                                                                                                    >  2:205985/1‑251 (MQ=255)
                 gAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTAcc                                                                                                                                                                                                                                                <  1:599202/237‑1 (MQ=255)
                 gAACTTGCAGGTTTGGTAGGGCCACAGGATGTGGAGCTCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGCTGATTAAGGAGAATACATGACGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTAcc                                                                                                                                                                                                                                                >  2:599202/1‑237 (MQ=255)
                     ttGCAGGTTTGTTAGGGCCACAGGATGAGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCac                                                                                                                                                                                                                              >  1:366110/1‑249 (MQ=255)
                      tgctggTTTGTTTGGGCACCAGGTTGTGGAGATCCGCATTCCTGCGCATCGTTGTGCTCGTATTCAGGAAATGCATATGCTGACGGTAAATTGCCGGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTGCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCaa                                                                                                                                                                                                                              <  2:480308/245‑1 (MQ=255)
                        cAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGcc                                                                                                                                                                                                                          >  1:257653/1‑251 (MQ=255)
                                  aGGGCCACAGGATGTTGAGATCAGCATTCCTTCGTATCGTAGTGCTAGCATTCAGGAAATGCATAGGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTAGCGCCAATCGCAGTCCTTATTGCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACTAAAGCCGCAACTGAc                                                                                                                                                                                                                 <  2:332143/250‑1 (MQ=255)
                                          aGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAgt                                                                                                                                                                                                        >  1:538967/1‑251 (MQ=255)
                                               ttgGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGTAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCg                                                                                                                                                                                                    <  2:40083/249‑1 (MQ=255)
                                                tgGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGAAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGTGTACCGCTGC‑TGTGGGTACCAAAGCCGCCACTGACCCACGCAGTGTCGGc                                                                                                                                                                                                  >  1:367011/1‑251 (MQ=255)
                                                           aTTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGCTCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGGGACTACATGAAGTCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGATACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTggc                                                                                                                                                                                       >  1:92563/1‑250 (MQ=255)
                                                             tCCTTCGCATCGTTGTGCTCGCAGTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTGTCCCTCACCAGGATGTTTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGGTGCGGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTCCCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGAc                                                                                                                                                                                      <  2:160661/250‑1 (MQ=255)
                                                               cTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGAt                                                                                                                                                                                   >  1:591408/1‑251 (MQ=255)
                                                                  cGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACTCGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCAGTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGt                                                                                                                                                                                <  1:567603/251‑1 (MQ=255)
                                                                  cGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGACTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATgg                                                                                                                                                                                 <  1:200550/250‑1 (MQ=255)
                                                                     aTCGTAGTGATCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGTGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTAcc                                                                                                                                                                             >  2:376700/1‑251 (MQ=255)
                                                                              cTCGCATACAGGAAATGCATATGCTGACGGAAAATTGCCAGTGCGATCTGATCGATAACACGATTTTCCCTCACCAGGATGATTAAGGAGAATCCATGACGGCATTATCGCCAATCGCAGTCCTTACTTCAGCGATGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCG‑TGCCTGTGGGTACCAAAGCAGCAACTGACCCCCGCAAGGTCGGCGCCAAGGTGGaagaggcttccacggacatt                                                                                                                                                                    >  2:66608/1‑232 (MQ=255)
                                                                               tCGCATTCAGGCAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGCGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTAACAAAGCAGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTg                                                                                                                                                                    >  2:428314/1‑250 (MQ=255)
                                                                                     tCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTg                                                                                                                                                              <  1:120801/250‑1 (MQ=255)
                                                                                       ccgatcggCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAGTACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAAc                                                                                                                                                           <  2:420182/244‑1 (MQ=255)
                                                                                             tGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCACTCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGAAACTGACCCACGCAGTGTCGGCACCCAGGAGGCCGATGGTACCCTGGATGGGCGCGTGaaaggcgcc                                                                                                                                                     >  2:614990/1‑244 (MQ=255)
                                                                                              gCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGATTACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAAc                                                                                                                                                           >  1:420182/1‑244 (MQ=255)
                                                                                              gCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCa                                                                                                                                                     <  1:376700/250‑1 (MQ=255)
                                                                                                atatGCTGTCGGTAAATTGCCTTGGCGATCTGATCGGAAACACGCTTTTCCCTCACCAGGATGATTAAGGGGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGATGTGCGCGTGAACAgcgaatgg                                                                                                                                                  <  2:110220/251‑6 (MQ=255)
                                                                                                  atGCTGACGGTAAATTGCCTTGGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGACCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTc                                                                                                                                                <  2:538967/251‑1 (MQ=255)
                                                                                                  atGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTc                                                                                                                                                <  1:219529/251‑1 (MQ=255)
                                                                                                        aCGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGaa                                                                                                                                             >  1:564652/1‑248 (MQ=255)
                                                                                                           gTAAATTGCCTGTGCGATCTGATCGATAACACGATTTTCCCTAAAAAGGATGATAAAGGCGAATACATGAAGGCATTATCGCCAATAGAAGTCCTTATTTCCGCGCTGCTGTTGAAA‑GGTT‑GTGTTGCTGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACACACGCAGTGTCGGAACCCAGGGGGACGATGGTACCCTGGACGTGCGCGTGAACAGCGCATTGTCGAAAGACg                                                                                                                                        >  1:603329/1‑250 (MQ=255)
                                                                                                           gTAAATTGACTGTGCGATCTGATCGATCACACGCTTTTCCCTCACCAGGATGATTACGGAGACTAACTGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGTTGCTGTTGCAC‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGTGTATCAAAGCCCCCAATGACCCACGCCGTGCCGCGACCCAGGGAGACGATGGTACCCAGGTAGTGAGCGtcaaaacaaccttgacgagaga                                                                                                                                          >  2:317898/1‑227 (MQ=255)
                                                                                                             aaaTTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGaa                                                                                                                                      <  1:566790/250‑1 (MQ=255)
                                                                                                                  gCCTGTGCGATCTGATCGATACCACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAACCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAAc                                                                                                                                                           <  2:211400/224‑1 (MQ=255)
                                                                                                                  gCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAAc                                                                                                                                                           >  1:211400/1‑224 (MQ=255)
                                                                                                                      gtgCTATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTaaga                                                                                                                            <  2:589049/251‑1 (MQ=255)
                                                                                                                        gCGATCTGATCGATAACACGCTTGTCCCTCACCGGGATGATTAAGGAGAATACAGGAAGGCATTATCGCCAAGCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGGG‑GTTTTGCCGCTGCCGTAATGGTTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTaagaaa                                                                                                                          <  2:43593/251‑1 (MQ=255)
                                                                                                                          gATCTGATCGATAACACGATTACCCCTCACCAGGATGATTAAGGCGCATACATGAAGGCATTATAGCCAATCGCCGACCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGACGCTGCCGCAATGGGTAACGCTGC‑TGTGGGTACCAAAGACGCAACTGCCCCACGCAGTGTCGGCACCCAGGCGGACGATGGTCACTTGGAAGTACGCGGTAACAGGGCATTGTCGGAAGACGAACGGAttgaagacg                                                                                                                         >  2:323361/1‑243 (MQ=255)
                                                                                                                            tCTGATCGATAACACGCTTTTCCCTCACCAGGCTGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGAAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCACATTGTCGAACGACGACCAGATAaagaaaga                                                                                                                        >  2:500114/1‑249 (MQ=255)
                                                                                                                               gATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAAGCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAgc                                                                                                                     <  2:308347/249‑1 (MQ=255)
                                                                                                                                aTCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAgcgcg                                                                                                                  >  1:11911/1‑251 (MQ=255)
                                                                                                                                aTCGATAACACGCTTTGCCCTTCCCAGGATGATTAAGAAGTATACATGAAGGCATTATCGCCAATCGCCGTCCTTATTTGCGCGCTGCGGTTGCA‑‑GGTTATTGCTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTATCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGTATTGACTAAAGACGACCAGATTAAGAAATAAgcgag                                                                                                                  <  2:32688/251‑3 (MQ=255)
                                                                                                                                   gATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCAt                                                                                                               >  1:675829/1‑251 (MQ=255)
                                                                                                                                   gATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCAt                                                                                                               >  1:560031/1‑251 (MQ=255)
                                                                                                                                    aTAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCCACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGCCGAACAGATTAAGAAAGAAGCGCGCAtt                                                                                                              >  2:626427/1‑251 (MQ=255)
                                                                                                                                                 cccTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTc                                                                                                                                                >  2:572719/1‑204 (MQ=255)
                                                                                                                                                 cccTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTc                                                                                                                                                <  1:572719/204‑1 (MQ=255)
                                                                                                                                                  ccTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTa                                                                                                <  1:339059/251‑1 (MQ=255)
                                                                                                                                                      aCCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATc                                                                                              >  1:193760/1‑249 (MQ=255)
                                                                                                                                                        cAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAggg                                                                                          >  1:471837/1‑251 (MQ=255)
                                                                                                                                                        cAGGATGAATAAGGAGAAGATATGAAGGCATTATCGCCAATCGCAGTCATTAGTTCCGCGCGTCTGTGGCAA‑GGTT‑GTGTGGCCGCTGCCGTAAGGGGTACCGCTGC‑TGTGGGTTCCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGTCTATCAggg                                                                                          <  2:255993/251‑1 (MQ=255)
                                                                                                                                                              gatTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGt                                                                                    >  1:119056/1‑251 (MQ=255)
                                                                                                                                                               atTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGCCGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGtg                                                                                   >  1:512088/1‑251 (MQ=255)
                                                                                                                                                               atTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGAGCTGCTGTTGAAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGCGGGTACCAACGCCGCAACTGACCCACGCAGTGTCGGCACACAGGCGGACGTTGGTACCCTGGAAGTGCGCGTGAAAAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAg                                                                                     >  2:600732/1‑249 (MQ=255)
                                                                                                                                                               atTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTGCCGCGCGGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGtg                                                                                   <  2:679424/251‑1 (MQ=255)
                                                                                                                                                                        aaTACATGAAGGCATTTTCGCCAATCGCAGGCCTTATTTCCGCGCTGCTGTTTCAA‑GGTT‑GTGTTGCTGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCATCTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGGGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCGGCTGGtt                                                                          <  2:248811/251‑1 (MQ=255)
                                                                                                                                                                            cATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCt                                                                                                 >  1:129164/1‑224 (MQ=255)
                                                                                                                                                                            cATGAAGGCAGTATCGCCAATCGCAGTCCTTAGTTCCGCGCGGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAAGGTAACGGCCt                                                                                                 <  2:129164/224‑1 (MQ=255)
                                                                                                                                                                             aTGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGc                                                                      >  1:452280/1‑250 (MQ=255)
                                                                                                                                                                               gAAGGCATTATCGCCAATCGCAGTCCTTATGTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑Gt                                                                  <  1:511054/251‑1 (MQ=255)
                                                                                                                                                                                aaGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGCACCAACGCCGAAACTGACCCACGCAGTGTCGGCACCCAGGAGAACGAGGGAACCCTGGCAGGGCGCGAGAACAGCGCAATATCGACAGACGAACAGATTCAAAAAGAAGAGCGAATTACTGTAACGGCCAATCAGGACAAAGTGCTGAAGGATGGGCA‑GTc                                                                 >  2:495021/1‑251 (MQ=255)
                                                                                                                                                                                  ggCATTATCGCCAACCGCATCCCTAATTTCCGCGCTGCGGTTGAAA‑GGTT‑GTGTTGCCGCTGCCGGAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCATGGCAAAGTGCTGCTGGTTGGGCA‑GTCAc                                                               <  2:71369/251‑1 (MQ=255)
                                                                                                                                                                                    cATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGAGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAc‑c                                                             >  2:498373/1‑250 (MQ=255)
                                                                                                                                                                                     aTTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑Ca                                                            >  1:542735/1‑250 (MQ=255)
                                                                                                                                                                                        aTCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATg                                                        <  1:477904/251‑1 (MQ=255)
                                                                                                                                                                                            ccAATCGCAGTCCTTATTTCAGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGCAATGGGTACCGCTGC‑TGTGGGTCCCCCCGCCGCCCCTGACCCACGCAGTGTCGGCCCCCAGGCGGACGATGGTACCCTGGACGTGCGCGTGAACACCGCATTGTCGAAAGACGCCCAGATTAAGAAAGACGCGCGCATTAATGTAACGGCATATCCGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTg                                                     >  1:284383/1‑250 (MQ=255)
                                                                                                                                                                                             cAATCGTAGTCCTTATTTCCGCGCTGCTGGTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGaa                                                   <  2:114650/251‑1 (MQ=255)
                                                                                                                                                                                                 cGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGACCttt                                               >  2:206704/1‑251 (MQ=255)
                                                                                                                                                                                                 cGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGAACttt                                               <  1:428314/251‑1 (MQ=255)
                                                                                                                                                                                                  gCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGAACTTTc                                              <  1:81592/251‑1 (MQ=255)
                                                                                                                                                                                                   cAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCTGAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGAACTGTCg                                             <  1:424549/251‑1 (MQ=255)
                                                                                                                                                                                                    aGTCCTTATTTACGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTAGAAAGACGCACAGATTAAGACAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGAACTTTCgg                                            >  2:647804/1‑251 (MQ=255)
                                                                                                                                                                                                       ccTTATTTCCGCGCTGCTGTTGCAA‑GGAT‑GTGTTGACGCTGCCGTTGCGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACCGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGCACTTTCGGCTc                                         >  1:236977/1‑251 (MQ=255)
                                                                                                                                                                                                                cgcgCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGAACTTTCGGCTCGCGCCAAAc                                >  1:248927/1‑251 (MQ=255)
                                                                                                                                                                                                                cgcgCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGAACTTTCGGCTCGCGCCAAAc                                >  1:395987/1‑251 (MQ=255)
                                                                                                                                                                                                                 gcgcTGCAGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTCGGTACCAAAGCCGCCACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGACCAGCGCATTGTCGAATGCCGAACAGATTACGACAGACGCGAGCCTTAATGTAACGTCCTATCCTGGCAAAGAGCTGCTGGTTGG‑CACGTCAC‑CAACTGCTGAACTTTCGGCTCCCGCCacac                                >  2:520609/1‑247 (MQ=255)
                                                                                                                                                                                                                   gctgctGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAGTGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCCGTGTCGGCACCCAGGTGGACGATGGTACCCTGGACGTGCGCGTGTACAGCGCATTGTCGAAAGACGAACAGATAAAGAAAGAAGCGCGCAATAAAGTAACGGCCTATAACGGAAAAGTGCTGATGGGTGGGAA‑GT‑ACACAAATGACGCACTTTCTGCTCGCGCAAAAcgt                              >  2:187448/1‑248 (MQ=255)
                                                                                                                                                                                                                   gcgctgttgtttagagggtgttttGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGGGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGAACTTTCGGCTCGCGCCAAACa                               <  2:127241/229‑1 (MQ=255)
                                                                                                                                                                                                                     tgctgTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGAACTTTCGGCTCGCGCCAAACAGAtt                           >  1:108257/1‑251 (MQ=255)
                                                                                                                                                                                                                      gctgTTGCCA‑GAGT‑GTGTTGCCACTGCCGTAACGAGTACCGCTGC‑TGTGGGCACCAAAGCCGCAACTGACCCACGCAGTGTCGGCCCCCAAGTGGACGATGGAACACTGGAAGCACGCGTGACAAGCGCATTGACGAAAGACGAACAGATTACGCAAGAAGCGCCCATTACTGGAACGGCCTAGCGAGGCAAAGTGCTGCTGGCTGGGCC‑GCCAC‑CAAATGCTGAAATTTCGGCTCgcgcgagaacgaat                           >  2:109402/1‑240 (MQ=255)
                                                                                                                                                                                                                        tgTTGCAC‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGGGGCCGATGGTACCATGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATTGAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGAACTTTCGGCTCGCGCCAAACAGaatggt                        >  2:623671/1‑246 (MQ=255)
                                                                                                                                                                                                                         gtagCAA‑GGGT‑GTGTTGCCGCTGACGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGGACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGAGAGCATTAATGTAACGGCCTATCATGGCAAAGAGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGAACTTTAGACTCGCGCCACACAGATGGCtt                       >  2:479890/4‑250 (MQ=255)
                                                                                                                                                                                                                         gTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCAACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGATGGTTGGGCA‑GTCAC‑CAAATGCTGACATTTCGGCTCGCGCCAAACAGATTg                          >  2:456778/1‑248 (MQ=255)
                                                                                                                                                                                                                          ttGCAATGGTG‑GTGTTGCCGTTTCCGTAATGGGTACCTCGGC‑TGTGGGGACCAATGCCGCAACTGACCCCCGCAGTGTCGGCACCCAGGTGGACGATGGTACCCAGGAAGTACGCGTGAACATCGCATTGGCGAAAGACGAACAGATTAAGAAAGAAGCTCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTTGGCA‑GTCTC‑CAAATGCTCAACTTTCGGCTCTCGCCAAACAGATTGCt                        <  2:242671/250‑1 (MQ=255)
                                                                                                                                                                                                                            gCAA‑GGTT‑GTGTTTCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAATTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGaa                                                                                                                                      <  2:540729/139‑1 (MQ=255)
                                                                                                                                                                                                                            gCAA‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGaa                                                                                                                                      >  1:540729/1‑139 (MQ=255)
                                                                                                                                                                                                                             cAA‑GGTT‑GTGTTGCCGCGGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATggg                   >  2:16401/1‑251 (MQ=255)
                                                                                                                                                                                                                              aa‑GGTT‑GTGTTGCCGCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGc                  >  1:286767/1‑251 (MQ=255)
                                                                                                                                                                                                                                   tt‑GTGTTGCCGCTGCCGTAATGGGTACCACTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTAGAAAGTCGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTAGCAGGGCAAAGTGCTGCTGGTTGAGCA‑GTCAC‑CAAATGATGACATTTCGGCTCGCGCCATACAGATTGCTATggggga                >  1:428251/1‑246 (MQ=255)
                                                                                                                                                                                                                                      ggtctGCCGCTGCCGTAATGGTTGCCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCTAGGTTGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGAACTTTCGGCTCGCGCCAAACAGATCTCTATGGGCGTAGa             <  2:547249/246‑1 (MQ=255)
                                                                                                                                                                                                                                              gCTGCCGTAATGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCaa    <  2:318625/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 gCCGTAATGGGGACCGCTGC‑TGGGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACg  <  2:542735/250‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
CCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAA‑GGTT‑GTGTTGCCGCTGCCGTAGTGGGTACCGCTGC‑TGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCA‑GTCAC‑CAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACG  >  NC_000913/3296236‑3296724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑