Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,934,092	G→A	intergenic (‑404/‑143)	fucA ← / → fucP	L‑fuculose‑phosphate aldolase/L‑fucose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,934,092	0	G	A	87.5%	33.9 / ‑2.0	16	intergenic (‑404/‑143)	fucA/fucP	L‑fuculose‑phosphate aldolase/L‑fucose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (8/6);  total (9/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

TAACCCCATTAAAAATGACCGCTCTTAAAAATATTTATCAAAACGGTCATTTTTCTATTCCTCCAAGCCCGGAATGACCGTTTTCGGCACAAACAATTAATACGGTCATCTGATTTGTGTTTTTTATGATTTATTTTCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAGAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCT  >  NC_000913/2933864‑2934326                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           tccctccacgccgccttcccgatctTAAAAATATTTAAAAAAACGGATATTTTTCTATTCCCCCAAGCCTGGAATGCCTGTTTTCGGCACAAACAATTAATACGGTAATCTGATTTGTGTTTTTTATTATTTATTTTCTGAAACGGTCTTGAAATGTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAGAGCGTTTTTTATTTGAAAATg                                                                                                                                                                                                                       <  2:161117/228‑1 (MQ=255)                 gACCGCTCTTAACCATATTTATCACAACGGTCATTTTCCTATTCCTCCAAGCCCGGAATGACCGTTTTCGGCACAAACAATTAATACGGTCATCTGATTTGTGTTTTTTATGATTTATTTTCTGAAACGGGCATGAAATTTCGATTATTAAAGTGCTGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTAATATAACAAAGAGTTTTTTATTTGAAAATGATTCCGTGAGTTCAttt                                                                                                                                                                                                      >  2:448219/1‑251 (MQ=255)                          tAAAAATATTTATCAAAACGGTCATTTTTCTATTCCTCCAAGCCCGGAATGACCGTTTTCGGCACAAACAATTAATACGGTCATCTGATTTGTGTTTTTTATGATTTATTTTCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAGAGCGTTTTTTATTTGAAAATg                                                                                                                                                                                                                       >  1:161117/1‑225 (MQ=255)                                                   ttttCTATTCCCCCAAGCCCGGAATGACCTGTTTCGTCACAAACAATTAATACGGTCATCTGATTTGTGTTTTTTATTATTTATTTTCTGAAACGGGCATGAAATTCCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCg                                                                                                                                                                     <  2:578932/250‑1 (MQ=255)                                                      tCTATTCCTCCAAGCCCGGAATGACCGTTTTCGGCACAAACAATTAATACGGTCATCTGATTTGTGTTTTTTATGATTTATTTTCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACt                                                                                                                                                                 >  1:350924/1‑251 (MQ=255)                                                                                                           cATCTGATTTGTGTTTTTTATGATTTATTTTCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGATTCTGa                                                                                                             >  2:582916/1‑250 (MQ=255)                                                                                                                                       tttCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGTATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATCACCGATTACGTGCTTAAGCTTCTGCATCTAAGAGGATGATATGGGAAACACATc                                                                                >  2:143716/1‑251 (MQ=255)                                                                                                                                                 gggCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGACCCTAAGAGGATGCTATGGGAAAAACATCAATACAAACg                                                                      >  2:527759/1‑251 (MQ=255)                                                                                                                                                 gggCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACg                                                                      >  1:629308/1‑251 (MQ=255)                                                                                                                                                  ggCATGTAATTGTGATTATTAAAGTTTTGTTAGCAACATAAATGCCCCTTCTTGTTAATAATTTAGACCGCCGTCATATTACAAAGCGTTTATTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCTCTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATATAAACGc                                                                     <  2:433950/251‑1 (MQ=255)                                                                                                                                                                   attaAAGTGATGGTAGTCACAAAAAGTCACCTTCTAGCTAATAAGTGTTACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGt                                                     <  2:350924/250‑1 (MQ=255)                                                                                                                                                                               gtattCACATAAATTCCCCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCa                                        <  1:446216/247‑1 (MQ=255)                                                                                                                                                                                          aaGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCAATTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAAAGCAGAGTTACCGTGCGGAAGCTAAGGATGAAGGGCAAAga                               >  1:125385/1‑248 (MQ=255)                                                                                                                                                                                                    cTAGCTAATAAGTGTGAACGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGCGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGATATGCACTTTTGAAAACATAACCGATTAAGAGCTTAATCTTCTGAACCTAAGAGGATGCTATGGGAAACACATAAATACAAACGCAGAGTTACCGTGCGGAAGATAAAGATGCATGGCAAAGCGGAAGTTACatt                   >  2:240207/1‑251 (MQ=255)                                                                                                                                                                                                                   gACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCgctg    <  1:448219/251‑1 (MQ=255)                                                                                                                                                                                                                     ccgccgTTATATTACAAAGCGATTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCgctgca  <  2:523501/251‑2 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           TAACCCCATTAAAAATGACCGCTCTTAAAAATATTTATCAAAACGGTCATTTTTCTATTCCTCCAAGCCCGGAATGACCGTTTTCGGCACAAACAATTAATACGGTCATCTGATTTGTGTTTTTTATGATTTATTTTCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAGAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCT  >  NC_000913/2933864‑2934326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑