Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,893,647 C→T Q331* (CAG→TAG)  ygcN → putative oxidoreductase with FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,893,6470CT99.0% 293.6 / NA 102Q331* (CAG→TAG) ygcNputative oxidoreductase with FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (45/55);  total (46/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

GACTCGCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCCAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTG  >  NC_000913/2893403‑2893892
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      
gacccGCTGTAAAGGGCATCCGGCTGTTCGTCCGCTTATGAAAAACTCGGGAGCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑TCTTGCACAGTATGCCGGTGCAAGACGCCGGTTACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACAGGAATGTCCGTGCGCGGCATGGATATTGCGCT‑GACTTGCGCGCAGGCGGCGGCAAAAACGCTG‑ATAA‑GTGCCTGCTAGCAc                                                                                                                                                                                                                                                   <  2:403395/246‑1 (MQ=255)
gACTCGCCTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCAcc                                                                                                                                                                                                                                                  <  2:269666/251‑1 (MQ=255)
 aCTCGCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACcg                                                                                                                                                                                                                                                 >  1:56153/1‑251 (MQ=255)
    cGCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACcgcg                                                                                                                                                                                                                                               <  1:312982/250‑1 (MQ=255)
          aaaGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGCATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGTCTGCTGGTGGACGATGCGTTGCGCAGTTGCGACAATACCGGAATTTCCGTGAGCGGCATGGATGAGGAGCT‑GACTGGCGCGCAGGCGGCGGCACAAAACCTG‑ACAA‑GCGCCTGATAGGACCGCGAGcagcc                                                                                                                                                                                                                                        >  2:469859/1‑247 (MQ=255)
                cATCCGGCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCt                                                                                                                                                                                                                                  >  1:8876/1‑251 (MQ=255)
                    cGGCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGttt                                                                                                                                                                                                                              >  1:252261/1‑251 (MQ=255)
                     ggCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTc                                                                                                                                                                                                                             >  1:432159/1‑251 (MQ=255)
                     ggCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGCGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTc                                                                                                                                                                                                                             >  1:522803/1‑251 (MQ=255)
                       cagtcgccccGCTTATCAAAAACCCGGAATAACTGGAGTATGGTGCGCATCGGGTGCCAGGAGGGG‑TCTGGCACAGGTGGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACGGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑TCGCCTTCTAGCACCGCGAGCCGCAAAATCGGttttcg                                                                                                                                                                                                                           <  2:432937/244‑4 (MQ=255)
                       cagggcgcACGCTTATCAAAAACTCTGAATCACTGGAGTATGGTGCGCTTCGGGTGCCAGAAGTTG‑GCTTGCACAGTATGCCGGTGCAAAACGCCGGTAACGGCTGGCTGCGGGTGGGCGATGCGTTGCGCAGTCGCGTCAATACCGGAATGTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCTCCGCGAGCCGCAAAATCTGTTTCCg                                                                                                                                                                                                                           <  2:41473/247‑1 (MQ=255)
                            cgcCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCttt                                                                                                                                                                                                                       >  1:163982/1‑250 (MQ=255)
                              cccGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTatc                                                                                                                                                                                                                    <  1:165433/251‑1 (MQ=255)
                                cGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTatcat                                                                                                                                                                                                                  <  1:1531/251‑1 (MQ=255)
                                cGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTatcat                                                                                                                                                                                                                  >  1:249739/1‑251 (MQ=255)
                                 gcgatcaaaaaaCACGGAATCACTGGAGTATGGTGCGCATTTGGTGCCCGATGGGG‑GCTTGCAAAGTAGGCGGGGGCAATACGCCGGTAACGGCGGGCTGCTGGGGGGCGATGCTTTGCGCATTTGCGTCAATACCGGAATGTCCGGGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCTGCACAAACGCTG‑ATAA‑GCCCCTGCTAGCACCGAGAGCCGCAAAATCTGTTTCCGCTTTatcaga                                                                                                                                                                                                                 <  2:103056/244‑3 (MQ=255)
                                     aTCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATcaca                                                                                                                                                                                                              <  1:578405/250‑1 (MQ=255)
                                             cacGGATTCACGGGAGTAGGGTGCGCATCTGGTGCCAGAAGGTG‑GTGTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCGGGCTGCGGGTGGGCGATGCGTTGCGCAGTTGCGTCAAGACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCCCCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAgc                                                                                                                                                                                                     <  2:284161/251‑1 (MQ=255)
                                             cacGGAATCACTGGAGTATGGTGCGCATCTGGTGCAAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGTTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGAGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCCAAATCTGTTTCCGCTTTATCATCACAACGGAGAgc                                                                                                                                                                                                     >  1:381014/1‑251 (MQ=255)
                                               cGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGGGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGAGAGCCGCAAGATCTGTTTCCGCTTTATCCTAACAACGTAGAgcg                                                                                                                                                                                                    >  2:479114/1‑250 (MQ=255)
                                                 gAATCACGTGAGTATGGTGCGCCTCTGGTGCCAGAAGGTG‑GCTTGCAAAGTATGCCGGTGCAATACGCCGGTAACGGTTGGCTGCTGGTGGGCGTTGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAg                                                                                                                                                                                                 <  2:249739/251‑1 (MQ=255)
                                                     cACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCctg                                                                                                                                                                                             >  1:15709/1‑251 (MQ=255)
                                                     cACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GAATGGCGCGCAGGCGGCGGCCCAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCAAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCctg                                                                                                                                                                                             >  2:603782/1‑251 (MQ=255)
                                                       cgggAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCctgct                                                                                                                                                                                           <  1:376580/249‑1 (MQ=255)
                                                       cTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAACCGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCCCAACGTAGAGCGCAGCccgct                                                                                                                                                                                           >  2:304236/1‑251 (MQ=255)
                                                       cTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCctgct                                                                                                                                                                                           >  1:221758/1‑251 (MQ=255)
                                                        gggagtcgtggtcgcctCTGGTGCCAGAAGGTG‑GCTTGCCTAGTATGCCGGTGAAATACGCCGGTACCGGCTGGCTGCTGGTGGGCGATGCGTGTCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACTCTG‑ATAA‑GCGCCTGCTAGCCCCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCctgctg                                                                                                                                                                                          <  2:151649/235‑1 (MQ=255)
                                                          gAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCtgtg                                                                                                                                                                                        <  1:373022/251‑1 (MQ=255)
                                                           agtagggggcgcATCTGGTGACAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGTGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GATTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTgg                                                                                                                                                                                       <  1:607788/243‑1 (MQ=255)
                                                            gTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTggg                                                                                                                                                                                      <  1:491344/251‑1 (MQ=255)
                                                                ggcgcgcAGCTGGTGCCAGAGGGGG‑GCTTGCACATTATGCCGGTGCAATACGCCGGTAACAGCTGGCTGCTGGGGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGTCGGCACAAACGCTG‑AGAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATg                                                                                                                                                                                   <  2:388998/247‑1 (MQ=255)
                                                                ggTGCGCATCTGGTGCCAGAAGGAG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGGTATGGCGCT‑GCCTGGCGCGCCAGCGGCGGCACAAACGCTG‑GTAA‑GCGCCTGCTAGCACCGCGAGCCGCCAAATCTGTTTCCGCTTTATCATCCCAGCGTAGAGCGCAGCCTGCTGTGGGCTGt                                                                                                                                                                                  >  2:467010/1‑251 (MQ=255)
                                                                    cgcATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GCCTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAACCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGACCGCAGCCCGCTGTGGGATGTTCt                                                                                                                                                                               >  2:546114/1‑250 (MQ=255)
                                                                     gcATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAACCGCTG‑ATAA‑GCGCCTGCAAGCACCGCCACCCGAAAGATCTGTTTACGATTTATCATAACAACGTAGAGGGCCGCCTGCTGTGGGATGTTCTAc                                                                                                                                                                             >  2:213720/1‑251 (MQ=255)
                                                                           ggTGCCAGAAG‑TGTGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGGGGGCGATGCTTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGTCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGc                                                                                                                                                                          <  2:432159/248‑1 (MQ=255)
                                                                               gccggaagggtgagtggacaGTATGCCGGTGCGATACGCCGGTAACGGCTGGCTGCGGGTGGGCGAGGCGGTGCGCAGTTGCGTCAATACCGGAATTTCCGGGCGCGGCATGGATATGGCGCT‑GACTGGCGCGGAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAGCGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATc                                                                                                                                                                    <  2:221758/234‑1 (MQ=255)
                                                                                   aaGGTG‑GCTTGCCCAGTATGCCGGTGCAATACGCCGGTAACGGCTGACTGCTAGTGGGCGATGCGTTGCGCCGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGCTATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGAACCGCGAGCCGCAAAATCTGTTTCCGCTTTCTCATCACAACGTAGAGCGCAGCCCGCTGTGGGTTGGTCTACAGCGTTATCAGcct                                                                                                                                                               >  2:501325/1‑249 (MQ=255)
                                                                                    aGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGACGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCAtt                                                                                                                                                              >  2:650189/1‑250 (MQ=255)
                                                                                      gTG‑GCTGGCACAGGATGCCGGTGCAATCCGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTGGCGCAGTGGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGtt                                                                                                                                                            <  2:151582/251‑1 (MQ=255)
                                                                                       tG‑GCTTGCACCGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCCAAATCTGTTTCCGCTTTATCATCCCAACGTAGAGCGCAGCCTGCTGTGGGGTGTTCTACAGCGTTATCAGCATGTT‑c                                                                                                                                                          >  2:310431/1‑251 (MQ=255)
                                                                                       tG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGAGGGCGATGCGTTGCGCAGTTGCGTCCATACCGGAATTTCCGTGCGCGGCGTGGATATGGCGCT‑GGCTGGCGCGCAGGCGGCGGCCCAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCCCAACGTAGAGCGACGCCCGCTTGGGGCTGGTCTACAGCGTTATCAGCATGTT‑c                                                                                                                                                          >  2:536521/1‑251 (MQ=255)
                                                                                                  agtagtccgggccattACGCCGGTAACGGGTGGCTGCTGGTGGGCGTTGCGTGGCGCAGTTGGGTCAAGACCGGAATTTCCGTGCGCGGCATGGAAAGGGCGCG‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCCCCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCATCATGTT‑CCGGCGCtttt                                                                                                                                                <  2:287512/236‑1 (MQ=255)
                                                                                                           gTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGccc                                                                                                                                       >  2:578895/1‑251 (MQ=255)
                                                                                                            tGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGCGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCg                                                                                                                                      >  1:33980/1‑251 (MQ=255)
                                                                                                              cAATCCGCCGGTACCGGCGGGCGGCTGGTGGGCGAGGCGTTGCGCAGTTGCGCAAATACCGGAACTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGAACAAACGCTG‑ATAA‑TCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCTCggg                                                                                                                                    <  2:163982/251‑1 (MQ=255)
                                                                                                              cAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCggg                                                                                                                                    >  1:448342/1‑251 (MQ=255)
                                                                                                               ccccaccggacgcttttccgatcttggtggGCGTGGCGTTGGGCTGTTGCGGCAATACCTGAATTTCCGTGCGCGGCAGGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCTCAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCggg                                                                                                                                    <  2:148679/226‑1 (MQ=255)
                                                                                                                      cGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTAcc                                                                                                                            <  1:390541/251‑1 (MQ=255)
                                                                                                                       ggTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCg                                                                                                                           <  1:618190/251‑1 (MQ=255)
                                                                                                                        gTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGt                                                                                                                          >  1:7813/1‑251 (MQ=255)
                                                                                                                         tAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGt                                                                                                                          >  1:103789/1‑250 (MQ=255)
                                                                                                                          aactgcggtgtgctgGTGGGCTCTGCGTTGCGCAGTTGCGTCAATCCCGGAATTGCCGTGGGCGGCAGGGATAGGGCGCT‑GGTTGGCGCGGAGGCGGCGGCATAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTTCCGTAc                                                                                                                        <  2:522803/241‑1 (MQ=255)
                                                                                                                             ggctggctGCTGGTGGGCGATGCGGTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTg                                                                                                                     <  1:213720/251‑1 (MQ=255)
                                                                                                                              gctggctgCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTgg                                                                                                                    >  1:525953/1‑251 (MQ=255)
                                                                                                                                 ggctgCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATCCCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCt                                                                                                                 <  1:314524/251‑1 (MQ=255)
                                                                                                                                    tgctgGTGGGCGATGCGTTGCGCAGTTGAGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAAAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATTTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTCCGTGGCCTGCg                                                                                                              >  2:489029/1‑251 (MQ=255)
                                                                                                                                       tggtggGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCggg                                                                                                                                    >  1:148679/1‑226 (MQ=255)
                                                                                                                                           gggCGATGTGTTGCGGTGTTGCGTCAATTCCGGAATTTCCGTGCGCGGATTGGATAGGGCTCT‑GACTGCCGCGCAGGCGGCGGCACAATCGCTG‑ATAA‑TCGCCTGGTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGc                                                                                                       <  2:8827/251‑1 (MQ=255)
                                                                                                                                             gCGATGCGTTGGGCAGTTGCGGCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAg                                                                                                     <  1:673142/251‑1 (MQ=255)
                                                                                                                                               gATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGCTATACCGCT‑GACTGGCGCGCAGACGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGGGGCCTGCGTTAATGCAgg                                                                                                    >  1:311970/1‑250 (MQ=255)
                                                                                                                                                       gcgcAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTccc                                                                                           <  1:407784/251‑1 (MQ=255)
                                                                                                                                                            gTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGAt                                                                                      >  1:20241/1‑251 (MQ=255)
                                                                                                                                                             gtGCGGCAAGACCGGAATTTCCGTGCGGGTAAGGGATATGGCGCG‑GACTGGCGCGGAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGAt                                                                                      <  2:7813/249‑1 (MQ=255)
                                                                                                                                                               gCGTTAATACCGGAATTCCCGTGCGCGGCATGGATATGGCGCT‑GACGGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCTTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACATCGTAGAGCGTAGCCTGATGTGTGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTa                                                                                   <  1:100772/251‑1 (MQ=255)
                                                                                                                                                                 gTCAATACCGGAATTTCCTTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATg                                                                                 >  1:117528/1‑251 (MQ=255)
                                                                                                                                                                     aTACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTCAGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATggg                                                                               >  2:165431/1‑249 (MQ=255)
                                                                                                                                                                       aCCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATc                                                                            <  1:578895/250‑1 (MQ=255)
                                                                                                                                                                         gggaagtcccgggcgcGGCATGGATATGGC‑CTAGACTGGCGCGCAGGCGGCGGCACAACCGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAgg                                                                         <  2:15709/239‑1 (MQ=255)
                                                                                                                                                                          ggaattccctggcgcGGCTGGGAGATGGCGCT‑GACTGGCGCGCGGGCGGCGGCATATACGTGG‑ATAG‑GCGCATTCTCGCCCCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAggg                                                                        <  2:449219/240‑1 (MQ=255)
                                                                                                                                                                                   gTGCGCGGCATGGATATGGCGCT‑GACTGGCGAGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTATTGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAAcc                                                               >  2:297586/1‑251 (MQ=255)
                                                                                                                                                                                    ggcgcGGAATGGATATGGCGCT‑GACTGGCGCGCAGGCGGAGGCACAAACGCTG‑ATAA‑GCGCTTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCt                                                              <  1:536521/250‑1 (MQ=255)
                                                                                                                                                                                     gcgcGGGATGGAGGTGGCGCC‑GACTGGCGCGCAGGCGGCGGCTAAAACG‑TGTATAA‑GCGCCTGCTTTCAGCGCGCGCCGCACAATCTGTTTCCGCTTTACCATCACATCTTCGAGCGCAGCCTGCTGTGTGATGTTCTTCAGTGTTATCAGCATGTT‑CCGGCGCTTTTGTAACGCCCGTGATGGTACCGTACGTGGCCTGCGTGAATGCAGGATATTTTCCGCGATTTATGGGATCAGGGTGATAAACCt                                                              <  2:268218/250‑1 (MQ=255)
                                                                                                                                                                                       gcgGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGtt                                                           <  1:304236/251‑1 (MQ=255)
                                                                                                                                                                                        cgGCATGGATATGGCGCC‑GTCTGGCGCGCAGGCGGCGGCACAAACGTTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTc                                                          <  2:252261/251‑1 (MQ=255)
                                                                                                                                                                                          gCATGGATATGGGGCT‑GACTGGCGAGCAGGCGGCGGCACAAACGATG‑ATAA‑GCGCCTGCTAGCCCCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCa                                                        <  1:670317/251‑1 (MQ=255)
                                                                                                                                                                                          gCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTcc                                                         >  1:429993/1‑250 (MQ=255)
                                                                                                                                                                                           cATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGGTAAACCTGTTCCAc                                                       >  1:406696/1‑251 (MQ=255)
                                                                                                                                                                                             gggATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTGCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCg                                                     <  2:552633/250‑1 (MQ=255)
                                                                                                                                                                                                         t‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGTTCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGtt                                          >  2:282636/1‑250 (MQ=255)
                                                                                                                                                                                                               ggCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGCTATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTTCGCCAGTTATTCTg                                    >  2:140061/1‑251 (MQ=255)
                                                                                                                                                                                                               ggCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTg                                    >  1:48885/1‑251 (MQ=255)
                                                                                                                                                                                                                gggggacggcggtggcctaaaCGCTG‑‑TAACGCGCCTGCCAGCACGGCGTGCCGCAAAATCTGTTTCCGATTTATCACCACATCGTAGAGCGCAGCCTTCTTTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTAACGTACGGGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTgg                                   <  2:448342/233‑1 (MQ=255)
                                                                                                                                                                                                                 cgcgcAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCcgc                                                                                         >  1:501702/1‑196 (MQ=255)
                                                                                                                                                                                                                        gcggcggcACAAACGCTG‑ATAA‑GCTCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCAttt                           <  1:149072/251‑1 (MQ=255)
                                                                                                                                                                                                                           gcggcACAACCGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGAGGTTCTACAGCGTTATCAGCA‑GTTGCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATGTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTAcg                        <  2:77587/251‑1 (MQ=255)
                                                                                                                                                                                                                             ggcACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGccc                                                                                                                                       >  1:469558/1‑138 (MQ=255)
                                                                                                                                                                                                                             ggcACAAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGccc                                                                                                                                       <  2:469558/138‑1 (MQ=255)
                                                                                                                                                                                                                                acaAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGtt                                                                                                                                                            >  1:447245/1‑115 (MQ=255)
                                                                                                                                                                                                                                acaAACGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCGGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTTTGGGATGTTCTACAGCGTTAGCAGCATGtt                                                                                                                                                            <  2:447245/115‑1 (MQ=255)
                                                                                                                                                                                                                                  aaactcgg‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCAc                 <  2:117528/244‑1 (MQ=255)
                                                                                                                                                                                                                                   aaCGCTG‑ATAA‑GCGCCTGCTTGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACg                <  1:601315/251‑1 (MQ=255)
                                                                                                                                                                                                                                     cGCTG‑ATAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGc              >  1:427963/1‑251 (MQ=255)
                                                                                                                                                                                                                                      cgcgtaTAA‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGc              <  1:310431/246‑1 (MQ=255)
                                                                                                                                                                                                                                         g‑ATAC‑GCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCtg           <  1:469859/250‑1 (MQ=255)
                                                                                                                                                                                                                                                gcgcCTGCTAGCCCCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCAt     <  1:489029/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 cgcCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTCCAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCAc                                                       >  2:631054/1‑200 (MQ=255)
                                                                                                                                                                                                                                                 cgcCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCAc                                                       <  1:631054/200‑1 (MQ=255)
                                                                                                                                                                                                                                                 cgcCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATc    >  1:333028/1‑251 (MQ=255)
                                                                                                                                                                                                                                                   ccTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTg  <  1:501325/251‑1 (MQ=255)
                                                                                                                                                                                                                                                   ccTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTg  <  1:546114/251‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      
GACTCGCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTG‑GCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCT‑GACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAA‑GCGCCTGCCAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTT‑CCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTG  >  NC_000913/2893403‑2893892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑