| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,893,219 | C→T | S188F (TCC→TTC) | ygcN → | putative oxidoreductase with FAD/NAD(P)‑binding domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,893,219 | 0 | C | T | 95.7% | 197.2 / ‑0.8 | 71 | S188F (TCC→TTC) | ygcN | putative oxidoreductase with FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (38/28); total (39/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01 | |||||||||||
TGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACGCAAAGTCGTGTTCCGGCAAGCGAGCTGCTGACTCGCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACGGAATCAC > NC_000913/2892983‑2893458 | cgccgaagccgaaATATAAGGTGGCGAAGGTATCCCCGGAGCGACGTTGGATGCACTGTATGAAGAAAACGGCAGAGT‑CTGTGGCGTTATTTGTTGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGGCCTGTCTGAACGTCACGTGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACAGTCGCTATTgaagaa < 2:441238/249‑1 (MQ=255) cgaagccgaaTAAGAAGGTGTCGAAAGCATCCCCGGAGCGTCTGTGGATGCACTTGATGAGGAAAACGGCA‑TGTGCTGTGGCGTTATTTGTGGTG‑ACGATAGTCGCCTCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCATGTGTTAGTGACTCGTCCTGCTGGCGAAGCGATGTCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAGGAACGtt < 2:601665/251‑1 (MQ=255) gaagccgaaAAAGAAGGTGTCGAATGCATCACCGGAGCGACGGTGGATGCACTGAATGAAGAAAACGGCGGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTTTCCGCGCCCGTTATGTGGTGCTGGCAGACGGTGCCAACAGTGTCCTGGCTGAAGGTCACGGGTTCGTGACTCGTCCTGCCGGCGAAGAGATTGCGTTGGGGATCCAAGAAGTGCTGACGCTGGACACATTCGCTAGTGAGGAACGttt > 2:135119/1‑251 (MQ=255) aaaaaGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGCTGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTg > 1:362306/1‑251 (MQ=255) acagaagGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGGTCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAAAGTTTTCATCTggg > 2:527914/3‑250 (MQ=255) aagaatGTGTCGAAAGCAGCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCATAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGgag < 2:527962/251‑1 (MQ=255) agaagGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTgg > 1:210036/1‑248 (MQ=255) agaagGTGTATAATGCACTCCCGGTGCGATGGTGGATGCGCTGTATGAAGAAAACGGCAGAGT‑CTGTGGCGTGATTTGTGGTG‑ACGAGATTCTCCGTGCCCTTTATGTGGTGTTGGCAGAGGGTGCCAACAGCGTGCTGGCTGATCGGCACGGGTTAGTGACTCGTCCTGTTGGCTAAGCGATGGCGTTGGGGATTAAAGTAGTGCTGGCGCCGGAAACAGTCTTTATGGAAGAACGTGTTCATCTGgaga < 2:74308/251‑1 (MQ=255) aagGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAAt > 1:396695/1‑251 (MQ=255) gggggcGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATaa < 1:16376/246‑1 (MQ=255) ggTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATaa < 1:595207/251‑1 (MQ=255) aaTGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGT‑CTGTGGCGTTAGTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGGGCCAACAGCGTCCTGGCTGAACGGCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAATAACGTTTTCATCTGGAGAATAACGAAGgc < 1:452880/251‑1 (MQ=255) gCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGgcgc > 1:89157/1‑250 (MQ=255) ggAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGGACGTTTTCATCTGGAAAATAACGAAGGCGCAGCGTTGCt > 2:520667/1‑251 (MQ=255) gACGGTGGATGCACTGTATGAAGAAAACGGCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTc > 1:100675/1‑250 (MQ=255) ggggATTCACGGTATTAAGAAGACGGCAGAGT‑ATGTGGGTTTATTT‑TGGGGTACGATATTCTCCGAGTCCGTTCTGGGGTGCTGGCAGAAGGTTCCAACAGCGTCCTGGCTGAACGTCACGGGTGAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGGCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCgg < 2:353893/249‑1 (MQ=255) tGGATGCACTGTATGAAGAAAACGGCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGc > 1:40103/1‑251 (MQ=255) gATGCACGGTATGAAGAAAACGGCAGAGT‑CTTTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGTCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAg < 1:289256/251‑1 (MQ=255) gCACTGTATGAAGAAAACGGCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGAt > 1:119074/1‑251 (MQ=255) aCTGTATGAAGAAAACGGCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAACCATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCt < 1:202198/251‑1 (MQ=255) gaaAACGGCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACtt > 1:432937/1‑251 (MQ=255) aaaCGGCAGAGT‑CTGTGGCGGTATGTGTGGGT‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTAc < 2:502988/251‑1 (MQ=255) cGGCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGACGGCGCAGGGATGCTGGTCAGCGGCAGGATCTGTGATGACTTACCcg > 2:157255/1‑251 (MQ=255) ggCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCa < 1:336913/188‑1 (MQ=255) ggCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCcgg > 1:284161/1‑251 (MQ=255) ggCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGAGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCa > 2:336913/1‑188 (MQ=255) gCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCcggc > 1:541662/1‑251 (MQ=255) cAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCcggcg > 1:499880/1‑251 (MQ=255) gT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAACGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGgcgc > 1:651765/1‑251 (MQ=255) tgtgGTGTTATTTGTGGTG‑ATGTGATTCTCCGCTCCCGTTATGTGGGGCTGGCAGAAGTTGCCAACTGCGTCCGTGCGGAACTTCACCGGGTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATAAAAGAAGTGCGGTCGCTGGAAACATGCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGTTCGGTGATGACTTACCCGTCGGCGCAt < 2:590817/250‑1 (MQ=255) gtgGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCAtt < 1:384364/250‑1 (MQ=255) gtgGCGTTATTTGTGGTG‑AAGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCACCAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTTTGATGACTTACCcggcgggccattt > 2:99076/1‑244 (MQ=255) gttcGTTATTTGTGGTG‑CCTCGATTCTCCGCTCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGACCGTGCTGAACGTCACGGTTTAGTGTCTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCTCAGGAATCATTCGCTATTGAAGACCGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCTGGATCTGTGATTACTTACCctgcgtctcatgt < 2:56064/247‑9 (MQ=255) ttATTTGTGGTG‑CCGATATTCTACGCGCCCGTTATGTGATGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGAGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGCTGACTTACCCGGCGGCGCATTTCTTtat > 2:308322/1‑251 (MQ=255) aTTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATAc < 1:288544/251‑1 (MQ=255) g‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGAATTACCCGGCGGCGCATTTATTTATACTAATcaac > 2:491344/1‑250 (MQ=255) agatTCTCCGCGCCCGATATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCAGCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGAGCTGTGATGACTTACCCGGCGGCGCATTTCGTTATACTAATCAACAAACg < 2:337455/249‑1 (MQ=255) ccGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGGCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGAGCAAAGAAGTGCTGGCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGctct < 2:312724/248‑1 (MQ=255) cccGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAggg > 1:584877/1‑251 (MQ=255) cccGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAggg > 1:271363/1‑251 (MQ=255) cGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATAATAATCAACAAACGCTCTCGTTAgggtt > 1:412090/1‑249 (MQ=255) aTGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGtt < 1:205172/251‑1 (MQ=255) gCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCAGTTCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCg < 1:575894/250‑1 (MQ=255) gCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTATCGTTAGGGATTGTTTGCCCGctctt > 2:111399/1‑250 (MQ=255) gggCCAACAGCGTCCTGGCTGAACGTCACTGGATAGTGACGCGTGCTGCTGGCGAAGCGATGGCGTTGGGGAGCAAAGAAGTGCTGTCGCTTGAAACATTCGCTATTGAAGAACGGTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGTATCTGTGATGACTTACCCGGTGGCGCATTTCTTTATTCTAATCAACAAACGCTCTCGTTAGGGATTGTTCGCCCGCTCTCTTcccgt < 2:100675/249‑3 (MQ=255) cAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCCCTCGTTAGGGAGTGTTTGCCCGCTCTCTTCCCTTCCGc > 2:543941/1‑251 (MQ=255) ccTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACGCAAAGTCgtg > 1:268218/1‑251 (MQ=255) gAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACGCAAAGTCGTGTTCCGGc < 1:70644/251‑1 (MQ=255) gAACGACACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACAAATCAAACAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACCCAAAGGCGTGTTCCGGc > 2:578405/1‑251 (MQ=255) gTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACGCAAAGTCGTGTTCCGGCAAGc > 1:403395/1‑251 (MQ=255) gtggctgggctgtctggctagtcggtGGCGTTGGGGCTCAAAGAAGTGCTGTCGCTTGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAGCAAACGCTCTCGTTAGGGATTGGTTGCCCGCTCTCTTCCCTTACGCAATGTCGTTTTCCGGCAAGCGAGTTGCTGAc < 2:204393/226‑1 (MQ=255) ccTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGCAGGCGCAGCGTTGCTGTTCAGCGTCAGGCTCTGTGCTGACTTACCCGGCGACGCCTTCCTTTATACTAAACAACACACGCTCTCGTTAGGGCTTGTTTGCCCGACCTCTTCCCTTACGCAAAGTCGTGTTCCGGCAAGCGAGAAGCTGACTCg > 2:241930/2‑251 (MQ=255) gTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACGCAAAGTCGTGTTCCGGCAAGCGAGCTGCTGACTCGCttt > 1:103056/1‑251 (MQ=255) tCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACGCAAAGTCGTGTTCCGGCAAGCGAGCTGCTGACTCGCttt > 1:266267/1‑250 (MQ=255) tCCTGCTGGCGAAGCGATGGCGTGGGGGATCAATGAAGTGCTGTCTCTGGAAACATTCGCTAGTGAAGAACGGTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAGGTATTGTTTGCCCGCTCTCTGCCCTTACGCAAAGTCGTGTTCCGGCAAGCGAGCTGCTGACTCGCtt < 2:541662/249‑1 (MQ=255) ccTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGTAGCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACGCAAAGTCGTGTTCCGGCAAGCGAGCTGCTGACTCGCTTTa < 1:425212/250‑1 (MQ=255) ccTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACGCAACGTCGTGTTCCGGCAAGCGAGCTGCTGACTCGCTTTa > 1:650694/1‑250 (MQ=255) cGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTGCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTGCCCTTACGCAAAGTCGTGTTCCGGCAAGCGAGCTGCTGACTCGCTTTAAAGCGCATc < 2:669727/251‑1 (MQ=255) gAAGCGACGGCGTTGGGGATCAAAGAAGCGCTGTCGCAGGAAAAATTTGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATAACTTACGCGGCGGCGCATTTCTTTATACTCATCAACAAACGCCCTCGTTAGGGATTGTTTGCCCGATCTCCTCCCTAAACCAGAGTCGGGTTCCGGAAAGCGCACTGCTGGCTGGCTTTTCAGCGCaccc > 2:678138/1‑248 (MQ=255) aaGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACGCAAAGTCGTGTTCCGGCAAGCGAGCTGCTGACTCGCTTTAAAGCGCATCCg < 1:78441/251‑1 (MQ=255) gATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACGCAAAGTCGTGTTCCGGCAAGCGAGCTGCTGACTCGCTTTAAAGCGCATCCGGc > 1:269666/1‑249 (MQ=255) ggCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATTCGCTATGGAAGAACGTGTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACGCAAAGTCGTGTTCCGGCAAGCGAGCTGCTGACTCGCTTTAAAGCGCATCCGGCAGTgc < 2:89157/251‑1 (MQ=255) gggATCAAATAAGTGCTTTCGCTGGAAACATTCGCTATTGAAGAACGGTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACTAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACGCAAAGTCGTGTTCCGGCAAGCGAGCTGCTGACTCGCTTTAAAGCGCATCCGGCAGTGCGCCCGCt < 2:499574/251‑1 (MQ=255) gaagTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACGCAAAGTCGTGTTCCGGCAAGCGAGCTGCTGACTCGCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTAACaaaa > 2:514567/1‑250 (MQ=255) agTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACGCAAAGTCGTGTTCCGGCAAGCGAGCTGCTGACTCGCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAac > 1:151649/1‑250 (MQ=255) gTGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACGCAAAGTCGTGTTCCGGCAAGCGAGCTGCTGACTCGCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAacac > 1:679215/1‑251 (MQ=255) tGCTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACGCAAAGTCGTGTTCCGGCAAGCGAGCTGCTGACTCGCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACg > 1:207927/1‑251 (MQ=255) cTGTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACGCAAAGTCGTGTTCCGGCAAGCGAGCTGCTGACTCGCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACgg > 1:338745/1‑250 (MQ=255) gggcGCTGGAAACATTCGCTATTGAAGAACGTTGTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTGTAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTGATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCGCTCGCTTCCCTTACGCAAAGTCGTGTTCCGGCAAGCGAGCTGCTGACTCGCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAactcgtaa < 2:372433/248‑7 (MQ=255) gTCGCTGGAAACATTCGCTATTGAAGAACGTTTTCACTTGGAGAATAACGAAGGCGCAGCGTTGCTGGTCAGCGGCAGGATCTGTGATGTCTTACCCGGCGGAGCATTTCTTTATACTAATCAACAATCGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACGCAAAGGCGTGTGCCGGCAAGCGAGCTGCTGACTCGCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACGGaa < 2:372454/250‑1 (MQ=255) gcgggAAACATGCTCTATGGAAGAACGGTTTCATCTGGAGAATAAGGAAGTCGCAGCGTTGCTGTTCATCTGCAGGAGCTGGGAGTACTTACCCGGCGGCGCATTACTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCTCGCTCTCGTCCCTCACGCAAAGGCGTGTTCCGGCAAGCGAGCGAGTGACTCGTTTTAAAGCGCGGTCGGCAGTGCGCCCGCTTATGAAAAACACGGTATCAc < 2:651765/248‑1 (MQ=255) | TGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGT‑CTGTGGCGTTATTTGTGGTG‑ACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAGCGTTGCTGTTCAGCGGCAGGATCTGTGATGACTTACCCGGCGGCGCATTTCTTTATACTAATCAACAAACGCTCTCGTTAGGGATTGTTTGCCCGCTCTCTTCCCTTACGCAAAGTCGTGTTCCGGCAAGCGAGCTGCTGACTCGCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACGGAATCAC > NC_000913/2892983‑2893458 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |