| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,851,821 | G→A | G274D (GGC→GAC) | hypB → | hydrogenase isoenzymes nickel incorporation protein HypB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,851,821 | 0 | G | A | 98.3% | 182.1 / NA | 60 | G274D (GGC→GAC) | hypB | hydrogenase isoenzymes nickel incorporation protein HypB |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (27/32); total (27/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGAC > NC_000913/2851576‑2852061 | cGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTTTCGAAGGGGAAGACAAACCACCGAAATATCCGCATATGTTTTCCGCCCCCTCGCTGATGCTGCTCAACAAAGATGACCTTTTGCCGTATCTCCACTTT‑GACGTTGAAAAAAGCATCGCCTGC‑GCCCGCGAAGTCAATCCCGAAATTGAAATCATACTTATTTCCGCCACCAGcgacga > 1:553735/1‑249 (MQ=255) gCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTg < 1:109819/251‑1 (MQ=255) cGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTTCGTTACCGGAGGGGAAGAAAAAACACTGAAATATCCCCCTATGTTTTCCCCCGCCCCCCTGGTGCTGCTCCACCAAGATGACCTGTTGCCGTATCTCCACTTTTGA‑GTTGAAAAATTCCTCCCCTCC‑GCCCCCGAAGTCCATCCCGAAAATGAAATCATCCTTTTTTTCGCCCCCCCCGACGAAGGGATGGACCCGTGGCTGAAc > 1:644192/1‑251 (MQ=255) gCCATCTTCGATCTCGGTGAAAAACACAAAGAGGCGGTGCTTTCCGTGACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCGCGCTGATGCTGCTCAACAAAGTTGACTTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCTTCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAActg < 1:449601/251‑1 (MQ=255) gATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGt < 1:365545/232‑1 (MQ=255) catcGGTGAACAACACAAAGGGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTCTCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTACCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCTACCAGCGACGAAGGGATGGACCAGTGGCTGAACGGGCTGGAGacac < 2:258282/249‑1 (MQ=255) cGGTGAAAAACACAAAGTGTCGGTGCTTTTCTGTACCGAAGTTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGTCCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGaca < 2:547994/247‑1 (MQ=255) aaaaaCACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCCCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGAtgt < 1:679509/251‑1 (MQ=255) ggCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTAGTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTc < 1:260714/250‑1 (MQ=255) gCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTGccc < 1:457122/251‑1 (MQ=255) gTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGc > 1:601573/1‑251 (MQ=255) cGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCAt > 1:502508/1‑251 (MQ=255) aCAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAAcc > 1:335629/1‑251 (MQ=255) aCAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAAcc < 1:595640/251‑1 (MQ=255) aacccACGGAAATAGCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAg < 2:33321/248‑1 (MQ=255) ccACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTAGCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCTCAAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCg < 1:628215/251‑1 (MQ=255) cACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGACATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACCGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGCCGGCCACCAgg > 2:352217/1‑248 (MQ=255) aaaTATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGAGAAAgcc > 2:242531/1‑249 (MQ=255) tCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGt > 1:401948/1‑251 (MQ=255) ccGCATATGTTTGCCGCCGCCGCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCATGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGt < 2:644192/250‑1 (MQ=255) atatGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCg > 1:104840/1‑251 (MQ=255) tatGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCgg > 1:476248/1‑251 (MQ=255) atGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGc > 1:388066/1‑251 (MQ=255) tttGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGACGACGTCTGCGGCAtt > 1:534282/1‑251 (MQ=255) gctgccgccTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCAGTGACGGCAACCAGGCGAAAGTCGCCGTCTGCGGCATTCa < 2:403061/247‑1 (MQ=255) ccgccgccTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAgc < 1:374114/251‑1 (MQ=255) ccgctcatcTGACGCTTCTCAACAAAGTTAACCTGTGGCCGTAGCTCTACATT‑TACTTTGAAAAGTTCATCGCCTGC‑GCCCGAGATGTCAATCCAGAAATGGAAATCATCCTTATTTCCGCCACCAGCGATGAAAGGATGGACTAGTGGCTAAACTGGCTGGAGACACAGCGATGTGCAGAGGCGTTCCCGGCTAGCTCCGCTCCAGTGACGGCAACCAGTCGAAAGTCGACGTCTGCGGCATTCAGcgcg < 2:74810/243‑1 (MQ=255) gcctctcggATGCTGCTCAACAAAGTTGTCCTTTCGCTGTATCTCAACTTT‑GACGTGGAAAAGTGCATTGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCTTTTAGCGCGAt < 2:581985/243‑1 (MQ=255) ccTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATg > 1:650770/1‑251 (MQ=255) gATGCTGCTCAACAAAGTTGCCCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTGCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGAGCTATGTCGAttt < 2:551257/251‑1 (MQ=255) gATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGAttt > 1:188331/1‑251 (MQ=255) ctgctCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCg < 1:391673/187‑1 (MQ=255) gctCAAAAAAGGTGACCTGATGCAGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGAGAAGTCACTCCAGAAATTGAAATCATCCTTATTTCCTCCACCAGCGACGAAGGGATGGACCAGTGGCTGCACTGGCAGGAGACCAAGAGCTGGGCATAGGCGTTCCCGGACAGATCCGCACCATTGACGGCGACCAGGCGAAAGGAGACGTCTGAGGCATTCAGCGCGCTGTAGATTGAACggt > 2:546289/1‑249 (MQ=255) aaaGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGc > 1:138797/1‑251 (MQ=255) gTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCCTCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGTCTGACCTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGTCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGAt < 1:499899/249‑1 (MQ=255) gTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGAt < 1:352217/249‑1 (MQ=255) ccAACTTT‑GACGTTGAAAAGTTCATCGC‑TGTTGCCCGCGAAGGCAATCCAGAAAGTGAAATCATCCTTATTCCCGCCACCCGCGACGATGGGATGGACCAGTGGCTTAACTGGCTGGAGACCCAGCGGTGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGCCGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGACAACGGTCAGc < 2:406508/250‑1 (MQ=255) cAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTc < 1:138641/247‑1 (MQ=255) ttt‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCcgcgc > 1:66676/1‑251 (MQ=255) actttGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTgg < 1:313239/248‑1 (MQ=255) aCGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCCGATCCGCACCATTGACGGCAACCAGGCGAAAGACGACGTCTGCGGCCTTCAGCGCGAAGTCGATTTAACGTTAGTCGGCAGATGCGATGCAAACGGTCAGCCGCGCTTgg > 2:151055/1‑251 (MQ=255) cGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTggg < 1:584572/251‑1 (MQ=255) ttGAAAAGGGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCCTATTTCCGCCACCAGCGGCGCATGGATGGACCAGTGGATGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGGCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGc < 2:661715/250‑1 (MQ=255) aaaaaGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGGGGCGGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAg < 2:502508/250‑1 (MQ=255) aTCGCCGGC‑GCCCGCGAAGTTAATCCAGAAAGTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTg < 1:304162/251‑1 (MQ=255) gCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCCATGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGCTCCGCACCATTGACGGCAACCAGGCGACAGTCGCCGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTCGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTAGGtcttggt > 2:635699/1‑244 (MQ=255) ccGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTgg > 1:104262/1‑251 (MQ=255) gAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTGGCttt < 1:242531/251‑1 (MQ=255) aGTCAATCCAGAAATGGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACGGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGc < 2:650770/251‑1 (MQ=255) aaTCCAGAAATTGAAATCATCCTTATTTACGCCACCAGCGACGAAGGGATGAACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGAAACCAGGCGACAGGCGACGTCTGCGGACTTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTAAACGTTGGCTTTGCCATg > 2:240307/1‑251 (MQ=255) aGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTTAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGt > 1:129349/1‑251 (MQ=255) tGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGCTGTGCATAGGCGTTCTCGTCCAGATCCGCACCATTGACGGCAACCAGGCGACAGTCGACGTCTGCGGCATTCAGCGAGATGTCGCTTTACCGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCTGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGtaatta > 1:656368/1‑250 (MQ=255) ttATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGTAATTAATGAAGCCGa > 1:29236/1‑249 (MQ=255) ttATTTACGCCACCAGCGACGAAGGGATGGCACAGTGGCAGAACTGGCTGGAGACACAGCGATGTGAAGAGGCGTTCCCGGCCAAATCCGCACCATTGACGGCAACAAGGCGAACGTCGACGTCTGCGGAATTCAGCGCGATGGCGATTTAACATTAGACGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGGGGGTACAGGAACACGGTGGCTTGGCCATTGGCGTAATTAATGAAGcctaa > 2:538625/1‑247 (MQ=255) tATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATAGTCGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTa < 1:454182/210‑1 (MQ=255) aTTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCa < 1:549402/251‑1 (MQ=255) ttCCGCCCCCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCAc < 1:350559/250‑1 (MQ=255) ttCCGCCACCAGCGACGAAGGGATGGCCCAGTGGCTGAACTGGCTGGAGACACAGCGATGTTCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCGGCTGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCAc < 2:388066/250‑1 (MQ=255) tCCGCCACCCGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCCGGCGAACGTCGACGTCGGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCTGCAGCTGCGATGACAACTGTCAGCCGCGCGTGGGCCAGTGGGTAATGGTACACGTTGGCTTTTCCATGAGCGTAATTACGTACGCCGAAGCAcgc > 2:279403/1‑251 (MQ=255) gCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGac > 1:494639/1‑251 (MQ=255) ccaccaGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGac > 1:152223/1‑250 (MQ=255) | CGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTT‑GACGTTGAAAAGTGCATCGCCTGC‑GCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGAC > NC_000913/2851576‑2852061 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |