Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,849,941	G→A	intergenic (‑92/+33)	hycB ← / ← hycA	formate hydrogenlyase subunit HycB/regulator of the transcriptional regulator FhlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,849,941	0	G	A	93.5%	249.9 / 2.7	95	intergenic (‑92/+33)	hycB/hycA	formate hydrogenlyase subunit HycB/regulator of the transcriptional regulator FhlA
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base A (39/47);  total (45/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.49e-01

TGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTT  >  NC_000913/2849695‑2850186                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      ggACAGAGCTGCGGCGTAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTa                                                                                                                                                                                                                                                   <  1:258220/250‑1 (MQ=255)   aCAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCTCGGCATTGATTGCAGGCCGTGCTGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAAc                                                                                                                                                                                                                                                 >  1:294109/1‑251 (MQ=255)   aCAGAGCGGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAAc                                                                                                                                                                                                                                                 <  1:399585/251‑1 (MQ=255)        gCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACCAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCCTCTTCGACACTCATCGACACGCCCATCCCCAAACAGCAGTCACGCCTg                                                                                                                                                                                                                                            >  2:48595/1‑251 (MQ=255)                           ttcattcaGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTc                                                                                                                                                                                                                          <  1:236803/250‑1 (MQ=255)                            ttattaaGCATCCCGCTCGGGCGAGGCATTAATTGCGGGCCGGGCTGGGGATG‑GTGTTCTGAACAGGTGGCTT‑CAAAAGGTTGGCAGCCGAT‑TCAGGGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACGCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCa                                                                                                                                                                                                                         <  2:313733/244‑1 (MQ=255)                             cattcaGCATCACTCTCAGGCGCGGCATTGATTGACGGCCGAGCTGGCGATGCGT‑CTCTGAAAAGGCGGCCT‑CGCAAGTGTGGCAGCCGAT‑ACAGAGCGAGGAGTCAGCAATTACAAAACGATTCACAAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCAGATGAAATGTCATTTTCGACACTCATCGACACGCACATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTctgc                                                                                                                                                                                                                       >  1:180699/1‑248 (MQ=255)                                      tCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCCAAGCCTCAGCTCAtgctgc                                                                                                                                                                                                              >  2:117929/1‑251 (MQ=255)                                       cACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGcc                                                                                                                                                                                                             <  1:238673/251‑1 (MQ=255)                                         ctctCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAACCCCCAGCTCATGCTGCCgg                                                                                                                                                                                                           >  2:487817/1‑251 (MQ=255)                                          tctcAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCggg                                                                                                                                                                                                          >  2:137789/1‑251 (MQ=255)                                          cctaagttggggcatgtatggCAGGCCGGTCTGGGGGTGCGT‑CTCTGAACAGGCG‑CCTGCCCAAGGGTGTCAGCCGAT‑ACAGGGCGTGGAGTCAGCAATTACAAAACGATTCCCCAGGC‑ATTCCTCAGGTGATTGCCATTTGTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGTGCAAAGCCTCAGCTCATGCTGACggg                                                                                                                                                                                                          <  2:473949/231‑1 (MQ=255)                                              aGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCttt                                                                                                                                                                                                      >  1:350041/1‑251 (MQ=255)                                                 cgcgGCATTGATTGCAGGCCGTGCTGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTc                                                                                                                                                                                                   >  1:340181/1‑251 (MQ=255)                                                    ggCATTGATTGCAGGCCGTGCTGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGAGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGACATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCt                                                                                                                                                                                                >  1:198530/1‑251 (MQ=255)                                                      cATTGATTGCAGGCCGTGCTGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGt                                                                                                                                                                                                    <  1:40750/245‑1 (MQ=255)                                                      cATTGATTGCAGGCCGTGCTGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGCGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGACGGGCTTTGtagat                                                                                                                                                                                                >  2:40750/1‑245 (MQ=255)                                                       attgattgCAGGCCGTGCTGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGACTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCttt                                                                                                                                                                                              >  1:528007/1‑250 (MQ=255)                                                           atggCAGGCCGTGCTGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAg                                                                                                                                                                                         <  1:301397/248‑1 (MQ=255)                                                             tgCAGGCCTTGCTGGCGAGGCGT‑CTCTGATCAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCATGTGATTGTCATTTTTGACGAAAACATGCCGATTAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGt                                                                                                                                                                                        <  1:315580/250‑1 (MQ=255)                                                                     gTGCTGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACCAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACCCTCATCGACACGCCCGTCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTaa                                                                                                                                                                                >  2:606687/1‑250 (MQ=255)                                                                         tGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCCGGTGATTGTCATTTTTGACGAAAACATGCCGATGAACTGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTc                                                                                                                                                                            >  2:162368/1‑250 (MQ=255)                                                                          cgggcatgcggccctGACCAGGCGGCCT‑ACCAAGTGTGGCTGCCGAT‑ACAGAGCGTGGATTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCCCAGGTGATTTTCATTTTTGAAGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCGTTGTCCCTTTACCAGTTGGCTTAAATTc                                                                                                                                                                            <  1:253982/237‑1 (MQ=255)                                                                            cgaggtgT‑CTCTGACCAGGGGGACG‑CACAAGTTTGGCATCCGAT‑ACAGTGCGTGGAGTCAGTATTGACAAACCTATTCAC‑AGGCGAGTCCTCAGGTGATTTTCTTTTTTGACGAAAACATGCCGATAAAATGTCATCTTCGACACTCATCGACACGCCCATCCCCAAACAGATGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGGCCCTTTACCAGTTGGATAAAATCCACCg                                                                                                                                                                        <  2:208886/245‑1 (MQ=255)                                                                            cGATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCg                                                                                                                                                                        <  1:123442/251‑1 (MQ=255)                                                                             gATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTCCCAGTTGGCTTAAATTCACCgg                                                                                                                                                                       >  2:281446/1‑251 (MQ=255)                                                                             gATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCCGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCgg                                                                                                                                                                       >  1:491179/1‑251 (MQ=255)                                                                                     cctcttaacacgctgctacacaAGTGTGGCAGCCGATTAC‑GAGAGTTGAGTCAGCTATTACAAAACGATTCAACAGGC‑AGTCCCCAGTTGATTGTCATTTTTGCCGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTATCGCCTGCAAAACGGGCAAAGCCTCATCTCATGCTGCCGGGCTTTGTCCCTTTACCTGTTTTCTTAAATTCACCGGCTCAtt                                                                                                                                                                 <  2:322591/233‑1 (MQ=255)                                                                                       cttaaCAGGCGGCCT‑CACAAGTGTGCCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACAtt                                                                                                                                                                 <  1:31055/245‑1 (MQ=255)                                                                                           aCAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCGCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCa                                                                                                                                                          <  1:337279/251‑1 (MQ=255)                                                                                             aGGCGGCCG‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGGTGAGTCAGCAATTACAAAACGATCCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAAt                                                                                                                                                        <  1:449938/251‑1 (MQ=255)                                                                                                gggcCC‑CACAAGGGTGTCAGCCTAT‑ACAGTGCGTGGAGTCAGCAATTACAAAAAGATGCTCCAGGC‑ATCCCCCAGGTTTTTGTCTTTTTTGACGTAAACATGCCGATGAAAGGTTATTTTCGACACTCATCTACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGTTCTTGCGGCCGGGCTTTGTCTCTTTACCAGGTGGCTTAAATTCACCTGCACATTTTTTTCATTAGc                                                                                                                                                     <  2:560689/250‑1 (MQ=255)                                                                                                              ggtggcagcatagaCAGAGCGTGGTGTCAGCATTTACAAAACGATTCACCATGC‑ATCCCTCAGGTTAGGGTCATTCTTGACGAAAACATGCCGATGAAATGGCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGTCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCGTTACCAGTTGGCTTAAATGCACCGGCACATTGTTTTCAATAGCGGTGGATAAGCt                                                                                                                                         <  2:343179/238‑1 (MQ=255)                                                                                                                      cGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAAc                                                                                                                                <  1:48595/251‑1 (MQ=255)                                                                                                                            aagagCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAg                                                                                                                           <  1:137789/250‑1 (MQ=255)                                                                                                                               agCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCg                                                                                                                         >  1:581836/1‑250 (MQ=255)                                                                                                                                 cGTGGAGTCATCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTTTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGAtt                                                                                                                      <  1:211729/251‑1 (MQ=255)                                                                                                                                  gTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAAAAGACGTAACGCCTGAAAAACGGGCAAAGCCTCAGCTAATGCGGCCGGGCTTTGTCCCTTTACCAGTGGGATTAAATTCACCGGCACATGGTTTTCAATAACGGGGTAGAAGCTGTCGTAAACACAAGCGAttt                                                                                                                     >  2:615578/1‑251 (MQ=255)                                                                                                                                      aGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTc                                                                                                                 <  1:52088/251‑1 (MQ=255)                                                                                                                                       gtcagaaatttaaaaaCTATTCACCAGGC‑ATTCCTTATGGGATTGTTATTTTTGACGAAAACATGTCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCAAGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCCATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCg                                                                                                                <  2:233404/239‑1 (MQ=255)                                                                                                                                        tCAGAAATTTCAAAACGATTCACCAGGC‑ATTCCACAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCAGCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAATCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTGGGCTTAAATTCACCGTCACAGTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCg                                                                                                                <  2:286000/250‑1 (MQ=255)                                                                                                                                         cAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGTCACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGGGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCggg                                                                                                              >  2:216657/1‑249 (MQ=255)                                                                                                                                              aTTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAgt                                                                                                         >  1:389156/1‑251 (MQ=255)                                                                                                                                                tACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGc                                                                                                                                                                                    <  1:483637/174‑1 (MQ=255)                                                                                                                                                tACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGc                                                                                                                                                                                    >  2:483637/1‑174 (MQ=255)                                                                                                                                                 aCAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGtt                                                                                                      >  1:160692/1‑251 (MQ=255)                                                                                                                                                  cAAAACGATTCACCAGGC‑ACTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTc                                                                                                     <  1:181759/251‑1 (MQ=255)                                                                                                                                                   aaaaCGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTcc                                                                                                    >  1:128019/1‑251 (MQ=255)                                                                                                                                                        gATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAAc                                                                                               >  1:60116/1‑251 (MQ=255)                                                                                                                                                        aaTTCAACAGGC‑ATTCCTCAGGGGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGCCGTAACGCCTGCAAAACGGGCAAAGCATCAGCTCATGCTGCCGTGCTTAGTCCCTTTACCAGTTGGATTAAATTCCCCGGCCCATTGTTTTCAATCACGGTGTATAAGCTGTCGTAAAAGCCAGCGATTTTTTCGCGATAGGGTTACAGaa                                                                                                >  2:445746/2‑250 (MQ=255)                                                                                                                                                         aTTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTGTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAAcc                                                                                              <  1:164203/251‑1 (MQ=255)                                                                                                                                                           tCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCt                                                                                             <  1:86619/250‑1 (MQ=255)                                                                                                                                                            cACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACgg                                                                                                                                                                                                                                    <  1:175354/114‑1 (MQ=255)                                                                                                                                                                aGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGCCGTACCGCCTGCAAAACGGGAAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCgg                                                                                                                                                                       >  2:77622/1‑171 (MQ=255)                                                                                                                                                                aGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTcgc                                                                                       <  1:48449/251‑1 (MQ=255)                                                                                                                                                                aGGC‑ATTCCCCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCgg                                                                                                                                                                       <  1:77622/171‑1 (MQ=255)                                                                                                                                                                     aTTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGGGTCTAAGCTTTCGTAATAGCCAGCGTTTTTTTCGAGATATTGTTCCAGAACCTGTTAGCGATc                                                                                   >  2:664456/1‑251 (MQ=255)                                                                                                                                                                       tCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCCACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATcg                                                                                  <  1:91321/250‑1 (MQ=255)                                                                                                                                                                         cTCAGGTGATTGTAATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCCTCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGACAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTAAACAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGGATATACGCTGTCGTAAACGCCAGCGATTTTTTCGAGTTAGTGTTCCAGCACCTGTTCGCGATCCCgg                                                                               >  2:473525/1‑251 (MQ=255)                                                                                                                                                                           cAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGAt                                                                             <  1:49368/251‑1 (MQ=255)                                                                                                                                                                                    gTCATTTTTGAAGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTGCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCtt                                                                    <  2:294109/251‑1 (MQ=255)                                                                                                                                                                                          tttGAAGAAAACATGCCGATGAAATGTAATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTAATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATTGCGGTGAATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGAGGCTGTTGACCTTGCCatc                                                              >  1:113837/1‑251 (MQ=255)                                                                                                                                                                                          ttggACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCat                                                               <  2:60116/247‑1 (MQ=255)                                                                                                                                                                                           ttGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCatc                                                              >  1:253893/1‑250 (MQ=255)                                                                                                                                                                                           ttGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCatc                                                              <  2:34754/250‑1 (MQ=255)                                                                                                                                                                                            tGACGAAAACATGCCGATGAAATGTCATTTTCGCCACTAATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTTAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGCTAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCatca                                                             >  2:287564/1‑250 (MQ=255)                                                                                                                                                                                             gACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCatcaa                                                            <  1:3223/250‑1 (MQ=255)                                                                                                                                                                                              aCGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCACTAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCatcaatc                                                          >  2:65021/1‑251 (MQ=255)                                                                                                                                                                                                  aaaCATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCAACCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGc                                                      <  2:581836/251‑1 (MQ=255)                                                                                                                                                                                                   aaCATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGcc                                                     >  1:441509/1‑251 (MQ=255)                                                                                                                                                                                                      aTGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTc                                                  >  1:57570/1‑251 (MQ=255)                                                                                                                                                                                                         cgtaagAAATGTCATTTTTGAAACTCATCCACACGACCATCCCCAAACAGACGTAGCTCCTGCAAAACGGGCAAAGCCGCAGCTCATGCTGCCGGGCGTTGTCCCTTTACCATGTGGCTCAAATTCACCGGCCCTTTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCTAGCGATTTTGTCGAGATAGTTTTCCAGAATCTGTTCGCGATCGCGTATGTTGATCTTGCCATCAATCATGCCGTCAAt                                               <  2:490026/246‑1 (MQ=255)                                                                                                                                                                                                          cGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGCCCTTGCCATCAATCATGCCGTCAATg                                              >  2:188972/1‑251 (MQ=255)                                                                                                                                                                                                            aagAAATGTCATTTTCGACACTCAGCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCt                                            <  2:328335/249‑1 (MQ=255)                                                                                                                                                                                                            aagAAATGCCATTTTCGACCCTCAACGACACGCCCATCCCCAAACAGACGTAACTCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCTGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATTGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTGGCCATCAATCATGCCGTCAATGCt                                            <  2:159499/249‑1 (MQ=255)                                                                                                                                                                                                            aTGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCt                                            >  2:38028/1‑251 (MQ=255)                                                                                                                                                                                                              gAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTc                                           >  1:275061/1‑250 (MQ=255)                                                                                                                                                                                                              gAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTc                                           >  2:263175/1‑250 (MQ=255)                                                                                                                                                                                                                 aTGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGtt                                       <  1:572714/251‑1 (MQ=255)                                                                                                                                                                                                                  tGTCATATTCGACACTCATCGACACGCCCATCACCACACAGACGTAAAGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTACCTATACCAGTTGGCTTACATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGGCGTAAACGCCAGCGATTTTTTCGAGTAAGTGTTCCAGAACCTGTGCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGcgcaattg                                      >  2:383262/1‑244 (MQ=255)                                                                                                                                                                                                                     cATTTTCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTgcgc                                   <  2:350041/251‑1 (MQ=255)                                                                                                                                                                                                                       ttttCGACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGAGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCCGCGATTTTTTCGAGATAGTGTGCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTTCCATCAATCATGCCGTCAATGCTCAGTTGCCCCt                                 >  1:403595/1‑251 (MQ=255)                                                                                                                                                                                                                          tCGACACTCATCGACACGCACATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCCCCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATCGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCCTGCCGTCACAGCTCAGTTGCGCCTgcg                              >  2:607779/1‑251 (MQ=255)                                                                                                                                                                                                                            gACACTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTgcgc                             >  1:548011/1‑250 (MQ=255)                                                                                                                                                                                                                                cTCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACTGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAAt                          <  1:49446/249‑1 (MQ=255)                                                                                                                                                                                                                                 tCATCGACACGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCg                       >  2:544777/1‑251 (MQ=255)                                                                                                                                                                                                                                      gACACGCCCATCCCCAAACAGATGTAACGCCTGCAAAAAGGGCAAAGCCTCAGCTCACGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGCTATTTTCGAGATAGTGTTCAAGAACCTGTTCACGATCGCGGATGTTGACCTTGCCATCAATCATGCCCTCAATACTCAGTTGCGCCTGCGCACTcctatttt                  >  2:102775/1‑245 (MQ=255)                                                                                                                                                                                                                                       acacGCCCATCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGttt                 <  1:216657/251‑1 (MQ=255)                                                                                                                                                                                                                                             ccATCCCCAAACAGACGTAACGCCTGCAAAACGGGCCAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATACGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTACCAGAACCTGTTCGCGATCGCGGATGTTGACCTGGCCCTCGATCATGCCGTCAATGCTCAGTTGCGCCTGCGCACTCCGCTGTTTGCCTTc           >  2:371221/1‑251 (MQ=255)                                                                                                                                                                                                                                               aTCCCCAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGTCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGAGGTTGTCCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGc         <  2:253464/251‑1 (MQ=255)                                                                                                                                                                                                                                                 ccccATACAGACGTAACGCCTGCAAAACGGGCATAGCCTCAGCTCATGCTGCCTGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGCCACATTGTTTTCAATAGCGTGGTAGAAGCTGTCGTAAACGCCAGCGATTTTCTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGTATGGTGACCTTGCCATCAAACATGCCGGCAATGCGCAGTTGCGCCTGCGCAAACCGCTGTTTGTCTTCGcc        <  2:71785/250‑1 (MQ=255)                                                                                                                                                                                                                                                   ccAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCATTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTTTTTGTCTTCGCCATc     <  2:198530/251‑1 (MQ=255)                                                                                                                                                                                                                                                   ccAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATc     <  1:615578/251‑1 (MQ=255)                                                                                                                                                                                                                                                    cAAACAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCt    <  1:607779/251‑1 (MQ=255)                                                                                                                                                                                                                                                      aaCAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCttt  >  1:244901/1‑251 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      TGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGT‑CTCTGAACAGGCGGCCT‑CACAAGTGTGGCAGCCGAT‑ACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGC‑ATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTT  >  NC_000913/2849695‑2850186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑