BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,818,331	G→A	intergenic (‑57/+142)	argY ← / ← argV	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,331	0	G	A	96.7%	79.6 / NA	30	intergenic (‑57/+142)	argY/argV	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (17/12); minor base C (1/0); total (18/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGC  >  NC_000913/2818118‑2818564

cAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGc                                                                                                                                                                                                      >  1:407051/1‑251 (MQ=255)

 aggttaGCTACGGATCCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCg                                                                                                                                                                                                     <  1:127795/246‑1 (MQ=255)

             gATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGAGATG‑TGTCATCCGGGGGGTTTACTCGCCTTGCGGCTCGCCCTTCg                                                                                                                                                                                         >  1:112993/1‑251 (MQ=255)

                 cATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGTATGCCTCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTCCTAAAGCCGTATCAACTCAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGccccttgcgtc                                                                                                                                                                                     >  2:312461/1‑243 (MQ=21)

                          atttatttTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCACATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGCACCGCCCCTCGGGCCGTTGCTaa                                                                                                                                                                             >  1:362473/1‑250 (MQ=255)

                             tatttTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCAGCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGAGATGGTg                                                                                                                                                                                                                                <  1:207099/197‑1 (MQ=255)

                                 ttACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGc                                                                                                                                                                                                           >  1:370942/1‑213 (MQ=255)

                                 ttACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGACGAGTACTCTATCAAGCTGAGCTACGGATGCATCGGGCAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGCGATGGAG‑AATCCGGGAGGATTACTCGCCTTGCGACTCGCCCCTCGGGCCGTGGCTAAAGCacctt                                                                                                                                                                     >  1:320278/1‑247 (MQ=21)

                                       ctgATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGGCCTTCGGGCCGTTGGTAAAGCacacgtatcct                                                                                                                                                               >  1:441682/1‑241 (MQ=255)

                                               gagtccGCTACCAAAGCCAGTTCATGTAATAGAGGGTGCAGCGGGTCGGATACGAACCTCCGTCCTCTCGGTTCGTTGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAGCGGGAAACTGATTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTAGCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGt                                                                                                                                                        <  2:351297/246‑1 (MQ=14)

                                               gATACCGCTCATAAAGCCATGTCAAGTAAGAGATGGGGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTg                                                                                                                                                       <  1:141492/251‑1 (MQ=255)

                                                 tACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTACGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACAGCTACTAAAGCAGTATCAAATAAGAGATGGCG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCGTCGGGACGTTGCTAAAGCAACGTTATCCTCCCttgggtc                                                                                                                                                     >  1:392290/1‑245 (MQ=18)

                                                             cgCCATATCAAGTAAGAGTTGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTAc                                                                                                                                         <  1:232242/250‑1 (MQ=255)

                                                                  tatCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCATCGGGCCGTTGCTTAAGCAACGGTATCCTCCCCGGTGCCCGCTGGTATTAcccccc                                                                                                                                    >  1:249221/1‑247 (MQ=17)

                                                                                    gCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGATGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCGGGCCCTTGCTAAAGCAAAGTTTTCCTCCCTGGTGCCTGCGGTTATAACCCCCACCTCTTCCATACCTTCAt                                                                                                                  >  1:656292/1‑251 (MQ=18)

                                                                                        ccgggagggcttgAACCTCCGACCGCGATGTTTGTAGCCTAGAACTCTAGCCCATTGAGCTACGGATGCATCGGGAATAGTACTTTACGCCCGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCt                                                                                                              <  2:456013/239‑1 (MQ=17)

                                                                                                   cGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTa                                                                                                   <  1:312461/251‑1 (MQ=255)

                                                                                                    gAACCTCCGACCGCTCGGTTCGTAGCCTAGTACACTATCCAGCTGAGCTACGGATTCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAg                                                                                                  <  1:677408/251‑1 (MQ=255)

                                                                                                             acctcccgcttCGTAGCCGAGTACCCTATCCAGCTGAGCTACGGATGCTTCGGGAAACTTACTTTTCTGCAGTTTTTTTGATACCTCTACGAGAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCCCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATgg                                                                                           <  2:203171/240‑1 (MQ=21)

                                                                                                                gCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCAt                                                                                      <  1:365059/251‑1 (MQ=255)

                                                                                                                                               taaccTACGTATCCATCGTGAAACTTACTTTACTGCGGATTTTTTGATCCCGCTCTTAAATCCTTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTGCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCgg                                                        <  2:441682/246‑1 (MQ=18)

                                                                                                                                               tGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGCCCGCTCGGt                                                       >  1:381531/1‑251 (MQ=255)

                                                                                                                                                     aCGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGGATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGGTCGTa                                                  >  1:387493/1‑250 (MQ=255)

                                                                                                                                                     aCGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGt                                                   <  1:459393/249‑1 (MQ=255)

                                                                                                                                                                           tttactGCAGATTTTTTGATACCGCTACTAAAGCAGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGGTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGAAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGCGGACTCTATCCAGCTg                             >  1:536159/1‑249 (MQ=255)

                                                                                                                                                                             tactGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGGGCCTGCGGTTATTACCGCCAACTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCAACCGGGAGGATTCGAAACCCCGACCCATCGGGTCGTAACCGAGTACTCTATCCAGCTGAGcc                         >  1:244952/1‑250 (MQ=18)

                                                                                                                                                                               ctGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCACCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTCCGGTTATTACCGCCACCTCTTCCATTCCTTCATCGCTGAAAGTACGTAGAATATGGGGCATCCGGGGGGATTCGAACCTCCGACCGCCCGGTTCGTAGCCGCGTACTCTTTCCCGCTggcctcc                       >  2:79562/1‑245 (MQ=18)

                                                                                                                                                                                                ccGCTACTAAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGGTATTACCGCCCCCTCTTCCATACCTTCATCGCTGAAAGTACGTAGTATATGGTGCATCCGGGGGGATTCGAACCTCCGACCGCCCGGTTCCTAGCCGGGTACTCTATACAGCTGAGCTAAGGATGCAAATGGCgggg      >  2:140874/1‑249 (MQ=17)

                                                                                                                                                                                                  gCTACTAAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTTTCCTCCCTGGTGCCCGCGGGTATTACCGCCACCTCTTCCATAACTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGAtt                                                                           >  1:99320/1‑180 (MQ=18)

                                                                                                                                                                                                    tactaAAGCCGTATCAAATAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGGGCCTTCGGTTATTACCGCCCCCTCTTCCATACCTTCATCGCTGAAAGGACGTAGAATATGGGGCATCCGGGAGGCTTCGAAACTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAggg  >  1:540844/1‑250 (MQ=18)

CAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTG‑CATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGC  >  NC_000913/2818118‑2818564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`