Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,097	T→C	S99S (AGT→AGC)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,097	0	T	C	97.3%	222.8 / ‑2.8	75	S99S (AGT→AGC)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (37/36);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01

GTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTG‑GGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTA  >  NC_000913/2855‑3341                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      gtgCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTc                                                                                                                                                                                                                                               <  1:538490/251‑1 (MQ=255)      aggggCAGCGGAGACACATGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCCAAGACATTCCGTCTCCACAACCTCGGACGCTTTGCCGCTAAGCTGCCGTCAGGACCACGGGAAAATCTCGTTTATCAGTGATGGGCGCGGTTTTGACAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATTCATCTGGAAAAGAATATGCCAATAGGTTCGGGCTTAGACTCCAGCGCCTGTTCggagg                                                                                                                                                                                                                                          >  2:449166/2‑248 (MQ=255)                gTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGAAGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGACAATCGGTTCAGGCTTAGGCTCCAGCGCCTTTTCGGGGGGcgcgtggct                                                                                                                                                                                                                                >  2:598968/1‑246 (MQ=255)                    cacCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATg                                                                                                                                                                                                                            <  1:180342/251‑1 (MQ=255)                    cacCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATg                                                                                                                                                                                                                            >  1:74589/1‑251 (MQ=255)                      ccTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGc                                                                                                                                                                                                                          <  1:598968/251‑1 (MQ=255)                            gATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑TGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTACGGTGGTCGCGGCGCTGATGGCGatgaa                                                                                                                                                                                                                    <  1:406982/251‑1 (MQ=255)                                gggcagtGCTCGGAGATGTAGTCACGGTGGAGGCGGCAGAGTCATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTATTTGCCAGGAGCTT‑GGCAAGCAAATGCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGAGGTCGCGGCGCTGATGGCGAtgaatgaa                                                                                                                                                                                                                <  2:73643/245‑1 (MQ=255)                                  gCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACAGTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTACGGTGGTCGCGGCGCTGATGGCGATGAATGAaca                                                                                                                                                                                                              <  1:176281/251‑1 (MQ=255)                                          cGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGc                                                                                                                                                                                                       >  1:595577/1‑250 (MQ=255)                                           ggagatgacgacACGGTTGAGGCGGCAGAGACATTCAGTCTCAAAAACCTCGGAAGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCATTTATCAGTGCTGGGAGCGTTTTTGCCCGGAGCTT‑GGCAAGCAACTTCCAGTGGCGAGGACTCTGGACAAGAATATGCCAATCGGTTCGGGCTTCGGCTACAGCGCCCGTTCGGTGGTCGCGGGGCTGTGGGGGATGAATGAAAACTgtggaa                                                                                                                                                                                                      >  2:70200/12‑245 (MQ=255)                                                tGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGc                                                                                                                                                                                                >  1:498765/1‑251 (MQ=255)                                                  tAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCtt                                                                                                                                                                                              >  1:636959/1‑251 (MQ=255)                                                  gaGTCACGGTTGAGGCGGCAGAGACATTCAGTCTAACCAACCTCGTAGGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATAGCGTTTATCAGTGCGGGGAGCGTGTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTGCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTgtttcaggtcgctt                                                                                                                                                                                              <  2:617875/250‑14 (MQ=255)                                                   aggcACGGTTGAGGCGGCAGAGAAATTCAGGCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCTGCAGATCCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCtt                                                                                                                                                                                              <  2:428810/247‑1 (MQ=255)                                                   aGTCGCGGTTGAGGCGGCAGAGACCTTCAGTCTCATCAACCTTGGACGCTTTGCCGATAAGCTGCCGTTAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGGTCGGGCTTAGGCTCCAGCGACTGTTCGGTGGTCGCGGCGCTGATGGAGATGAATGAACACTGTGGCAAGCCGCtt                                                                                                                                                                                              <  2:500776/250‑1 (MQ=255)                                                        cGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGac                                                                                                                                                                                        <  1:647014/251‑1 (MQ=255)                                                        cGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTG‑GGTAAGCAAATTCCAGTGGCGATGACCATGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGac                                                                                                                                                                                        >  2:342319/1‑251 (MQ=255)                                                         ggTTGAGGCGGCCGAAACAATTAGACTCAACAACCTCGGACGCTTTGACGATAAGCAGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTATTTGCAAGGAGCTT‑GGCAAGCAAATTACAGTGGCGATGACTATGGAACAGTATATGCCAATCGGTTCGGGCTTAGGCTCCGGCGCCAGTTAGGTGGTCGCGGCGCTGATGGCGCTGAATGAaccgtgtggcaaagcgggtaatgac                                                                                                                                                                                        >  2:29444/1‑227 (MQ=255)                                                         ggTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGacc                                                                                                                                                                                       >  1:453374/1‑250 (MQ=255)                                                            tGAGGCGGCAGAGACATTCAGTCGCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACt                                                                                                                                                                                     <  1:301041/250‑1 (MQ=255)                                                                        gaCATTCAGTCTCAACAACCGCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCtt                                                                                                                                                                        <  2:575287/251‑1 (MQ=255)                                                                                gTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGCTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACCCTCGTTTGCTGGCTTTGATGGGc                                                                                                                                                                >  1:28355/1‑251 (MQ=255)                                                                                   ccaacaaCCTCGGACGCGTTGCCGAGAAGCTGCCGTCAGAACCACGGGAAAATAGCTTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTG‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTGTGATGGGC‑GAg                                                                                                                                                            <  2:401258/250‑1 (MQ=255)                                                                                   acgctctgccgatccGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGTCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCGTTGATGGGC‑GAg                                                                                                                                                            <  2:263210/237‑1 (MQ=255)                                                                                    caacagCCTCGGACGCTTTGCCGATAAGCGGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTGTGCTGGCTTTGATGGGC‑g                                                                                                                                                              <  2:74589/248‑1 (MQ=255)                                                                                      acaacCTCTGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCACGCAAATTCCAGTGGCGATGACTCTGGTAACGAATATGCCAATCGGTTCGGGCTTAGGCTCCCGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCACGCCGCTTAATGACACTCGTTTGCTGGCTTTTAAGGGC‑GATCTg                                                                                                                                                         >  2:396453/1‑251 (MQ=255)                                                                                             cGGACGTTTTGCCGTTAAGCTGCCGTCCGAACCACGGGAAAATATCGTTTAGCAGTTCTGGGAGCGTTTTTGCCAGGA‑GTGTGGCAAGCAAAGTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAGCACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAggac                                                                                                                                                  <  2:631281/251‑3 (MQ=255)                                                                                                  gCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAg                                                                                                                                                            >  1:263210/1‑236 (MQ=255)                                                                                                  gCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAggacgaatc                                                                                                                                             >  1:21446/1‑244 (MQ=255)                                                                                                     cacgctcgcccgatccttcaaaaCCACGGGTAAATAGCGTTTATCAGTGCGGGTAGCGTTTTTGCCAGGAGCTT‑GGCAATCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATTACACGCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTcc                                                                                                                                          <  2:378012/230‑1 (MQ=255)                                                                                                         cGATAAGCTGCCGTCAGATCCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGgca                                                                                                                                      <  1:475998/251‑1 (MQ=255)                                                                                                            tAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGACAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGGTTAATGCCACTCGTTTTCTGGCTTTTATGGGCCGA‑CTGACAGGACGAATCTCCGgcagcc                                                                                                                                   >  2:452553/1‑250 (MQ=255)                                                                                                              aGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGcatt                                                                                                                                 >  1:287581/1‑251 (MQ=255)                                                                                                                     gTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAAtctca                                                                                                                                          >  1:378012/1‑234 (MQ=255)                                                                                                                     gTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACg                                                                                                                          >  1:46403/1‑251 (MQ=255)                                                                                                                     gTCAGAACCACGGGAAAATATCGTTTAGCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAAGCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCGGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACg                                                                                                                          <  1:618009/251‑1 (MQ=255)                                                                                                                            ccACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGt                                                                                                                   >  1:65022/1‑251 (MQ=255)                                                                                                                            ccACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGt                                                                                                                   <  2:636959/251‑1 (MQ=255)                                                                                                                              aCGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATTGGC‑GAGCTGGAAGGACGAATCTCCAGCAGCATTCATTACGACAACGTgg                                                                                                                 <  1:501287/251‑1 (MQ=255)                                                                                                                                gggAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTG‑GGTAAGCAAATTCCAGTGGCGATGACCATGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCa                                                                                                               <  1:342319/251‑1 (MQ=255)                                                                                                                                     aaTATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCgtg                                                                                                          <  1:137465/251‑1 (MQ=255)                                                                                                                                       tatCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCCAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCCATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGGACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGCAGGACGAATCTCCGGCAGCCTTCCTTACGACAACGTGGCACCGGGtt                                                                                                        >  2:381404/1‑251 (MQ=255)                                                                                                                                            tttATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAAt                                                                                                                                                                                           <  1:675676/164‑1 (MQ=255)                                                                                                                                                     gcggggAGCGTTTGTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATg                                                                                          <  2:287581/248‑1 (MQ=255)                                                                                                                                                     gCTGGGATCGTTTTTGCCCGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTTGAAAAGAATATGCCAATCGGTTCGGGCGTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGTGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATg                                                                                          <  2:21446/251‑1 (MQ=255)                                                                                                                                                     gCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAACAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGACTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGacac                                                                                                                                                                                      >  2:76827/1‑160 (MQ=255)                                                                                                                                                     gCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGacac                                                                                                                                                                                      <  1:76827/160‑1 (MQ=255)                                                                                                                                                          ggaggttttgtgcaaggagcttggTAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGATTTAGGCTCCAGCTCCTGTTCGGTGGTCGCGGCTCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGt                                                                                      <  2:486787/229‑1 (MQ=255)                                                                                                                                                              gTTTTTGACAGGAACTT‑GGCAAGCAAATTCCCGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGGTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGAAGAATCTCCGGCAGCATTCATTACGACAGCGTGACACCTGGTTTTCTTGGTGGAATGCAGTtgatg                                                                                 >  2:615022/1‑251 (MQ=255)                                                                                                                                                                ttttGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTtgatga                                                                                <  1:591318/250‑1 (MQ=255)                                                                                                                                                                     ccAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGATGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCga                                                                            >  1:434613/1‑249 (MQ=255)                                                                                                                                                                     ccAGGAACTT‑GGAAAGCAACTTCCAGTGGCGATGACTCTGGTAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGCATGAACAATGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTTGCAGGACGAATCTCCGGCAGCATTCATTACGCCAACGTGGCACCTTGTTTTCTTGGAGGTATGCAGTGTATGATCga                                                                            >  2:248952/1‑249 (MQ=255)                                                                                                                                                                                  caaGCAAATGCCAGTGGCGATGACTCTGGAAAATAATATGCCAAGCGGTTCGGGCTTAGGCTCCAGCGCCTGTGCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCGTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGAcatca                                                              <  2:453374/250‑1 (MQ=255)                                                                                                                                                                                   aaGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTGGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGCTGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGAcatcat                                                             >  1:566781/1‑251 (MQ=255)                                                                                                                                                                                     gCAAATTCCAGTGGCGATGACTCTGGAGAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGCACACTGTGGCCAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGACTCTCCGGCAGCAGTCATTAGGACTACGTGGCACCGTGTTTTCTTGGAGGAATGCAGTTGATGATCGAAGAAAACGAcgacatc                                                            >  2:387716/1‑244 (MQ=255)                                                                                                                                                                                              aGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCa                                                    >  1:209199/1‑249 (MQ=255)                                                                                                                                                                                                tGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGCCGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGGGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCaaagg                                                >  2:137276/1‑248 (MQ=255)                                                                                                                                                                                                tGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCaa                                                   <  1:137276/248‑1 (MQ=255)                                                                                                                                                                                                 ggCGATGACTCTGGAAAAGACTATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGc                                               >  1:487224/1‑251 (MQ=255)                                                                                                                                                                                                 ggCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGc                                               <  1:449166/251‑1 (MQ=255)                                                                                                                                                                                                    gATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGAGCTGATGGCGATGAATGAAAACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GCGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAAAGTGGCACCGAGTTTTATTGGTGGTATGCAGTTGATGATCGAAGAAAAAGACATCATCAGCCAGAAAGTGcc                                              >  2:496142/1‑249 (MQ=255)                                                                                                                                                                                                             ggAAAAGAATATGCCAATCGGGTCGGGCTTAGGCTCCAGCGGCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTtga                                     <  2:321868/249‑1 (MQ=255)                                                                                                                                                                                                              gAAAAGAATGTGCCAATCGGTTCGGGCTTAGGCCCCAGCGCTTTGTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACGCTGTGGCAAGCCTCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GTGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCTAGTGCCAGGGTTtgatga                                  <  2:434613/251‑1 (MQ=255)                                                                                                                                                                                                                        tGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTgg                        >  1:611675/1‑251 (MQ=255)                                                                                                                                                                                                                          ccactCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTgggg                      >  2:199385/5‑250 (MQ=255)                                                                                                                                                                                                                                  ttCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGgcag                                                                                                                                     >  2:127663/1‑132 (MQ=255)                                                                                                                                                                                                                                  ttCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGgcag                                                                                                                                     <  1:127663/132‑1 (MQ=255)                                                                                                                                                                                                                                    cGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTAt            >  1:292297/1‑251 (MQ=255)                                                                                                                                                                                                                                    cGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGAC‑GAGCTGGAAGGACGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGAGGCTGTGGGTGCTGGCGTAt            >  1:117678/1‑251 (MQ=255)                                                                                                                                                                                                                                      ggCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATc                                                                              >  2:121763/1‑183 (MQ=255)                                                                                                                                                                                                                                      ggCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATc                                                                              <  1:121763/183‑1 (MQ=255)                                                                                                                                                                                                                                        cTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCgg        >  1:536996/1‑251 (MQ=255)                                                                                                                                                                                                                                           aGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCgggg      <  1:381404/250‑1 (MQ=255)                                                                                                                                                                                                                                               tCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGGGTGGCTGTGGGTGCTGGCGTATCCGGGGATTa  >  1:281530/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      GTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTG‑GGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTA  >  NC_000913/2855‑3341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑