Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,073	G→A	P91P (CCG→CCA)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,073	0	G	A	97.0%	191.9 / ‑2.4	68	P91P (CCG→CCA)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (29/36);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.43e-01

CCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTG‑GGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGG  >  NC_000913/2826‑3319                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                        ccAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATc                                                                                                                                                                                                                                                      <  1:412413/251‑1 (MQ=255) ccAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATc                                                                                                                                                                                                                                                      <  1:331953/251‑1 (MQ=255)                 cGTCGGGTTTGGTGTGAGCGGGGCGGCGGTGACACCTGTGGATGGTGCATTGCTTGGAGATGTAGTCACGTTTGAGGCGGCAGAGACATTCAGTCTCAAAAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATAGCGTTTATCAGTGCTGGGAGCGTGTTTGCCAGGAGCTG‑GGCAAGCAAATTCCATTGGCGATGACTCTGGAAAAGAATTTGCCAATCGGTTCGGGCTTAGGCt                                                                                                                                                                                                                                      <  2:34952/251‑1 (MQ=255)                              gtgCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTc                                                                                                                                                                                                                         <  1:538490/251‑1 (MQ=255)                                   aggggCAGCGGAGACACATGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCCAAGACATTCCGTCTCCACAACCTCGGACGCTTTGCCGCTAAGCTGCCGTCAGGACCACGGGAAAATCTCGTTTATCAGTGATGGGCGCGGTTTTGACAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATTCATCTGGAAAAGAATATGCCAATAGGTTCGGGCTTAGACTCCAGCGCCTGTTCggagg                                                                                                                                                                                                                    >  2:449166/2‑248 (MQ=255)                                             gTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGAAGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGACAATCGGTTCAGGCTTAGGCTCCAGCGCCTTTTCGGGGGGcgcgtggct                                                                                                                                                                                                          >  2:598968/1‑246 (MQ=255)                                                 cacCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATg                                                                                                                                                                                                      <  1:180342/251‑1 (MQ=255)                                                 cacCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATg                                                                                                                                                                                                      >  1:74589/1‑251 (MQ=255)                                                   ccTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGc                                                                                                                                                                                                    <  1:598968/251‑1 (MQ=255)                                                         gATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑TGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTACGGTGGTCGCGGCGCTGATGGCGatgaa                                                                                                                                                                                              <  1:406982/251‑1 (MQ=255)                                                             gggcagtGCTCGGAGATGTAGTCACGGTGGAGGCGGCAGAGTCATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTATTTGCCAGGAGCTT‑GGCAAGCAAATGCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGAGGTCGCGGCGCTGATGGCGAtgaatgaa                                                                                                                                                                                          <  2:73643/245‑1 (MQ=255)                                                               gCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACAGTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTACGGTGGTCGCGGCGCTGATGGCGATGAATGAaca                                                                                                                                                                                        <  1:176281/251‑1 (MQ=255)                                                                       cGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGc                                                                                                                                                                                 >  1:595577/1‑250 (MQ=255)                                                                        ggagatgacgacACGGTTGAGGCGGCAGAGACATTCAGTCTCAAAAACCTCGGAAGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCATTTATCAGTGCTGGGAGCGTTTTTGCCCGGAGCTT‑GGCAAGCAACTTCCAGTGGCGAGGACTCTGGACAAGAATATGCCAATCGGTTCGGGCTTCGGCTACAGCGCCCGTTCGGTGGTCGCGGGGCTGTGGGGGATGAATGAAAACTgtggaa                                                                                                                                                                                >  2:70200/12‑245 (MQ=255)                                                                             tGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGc                                                                                                                                                                          >  1:498765/1‑251 (MQ=255)                                                                               tAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCtt                                                                                                                                                                        >  1:636959/1‑251 (MQ=255)                                                                               gaGTCACGGTTGAGGCGGCAGAGACATTCAGTCTAACCAACCTCGTAGGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATAGCGTTTATCAGTGCGGGGAGCGTGTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTGCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTgtttcaggtcgctt                                                                                                                                                                        <  2:617875/250‑14 (MQ=255)                                                                                aggcACGGTTGAGGCGGCAGAGAAATTCAGGCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCTGCAGATCCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCtt                                                                                                                                                                        <  2:428810/247‑1 (MQ=255)                                                                                aGTCGCGGTTGAGGCGGCAGAGACCTTCAGTCTCATCAACCTTGGACGCTTTGCCGATAAGCTGCCGTTAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGGTCGGGCTTAGGCTCCAGCGACTGTTCGGTGGTCGCGGCGCTGATGGAGATGAATGAACACTGTGGCAAGCCGCtt                                                                                                                                                                        <  2:500776/250‑1 (MQ=255)                                                                                     cGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGac                                                                                                                                                                  <  1:647014/251‑1 (MQ=255)                                                                                     cGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTG‑GGTAAGCAAATTCCAGTGGCGATGACCATGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGac                                                                                                                                                                  >  2:342319/1‑251 (MQ=255)                                                                                      ggTTGAGGCGGCCGAAACAATTAGACTCAACAACCTCGGACGCTTTGACGATAAGCAGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTATTTGCAAGGAGCTT‑GGCAAGCAAATTACAGTGGCGATGACTATGGAACAGTATATGCCAATCGGTTCGGGCTTAGGCTCCGGCGCCAGTTAGGTGGTCGCGGCGCTGATGGCGCTGAATGAaccgtgtggcaaagcgggtaatgac                                                                                                                                                                  >  2:29444/1‑227 (MQ=255)                                                                                      ggTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGacc                                                                                                                                                                 >  1:453374/1‑250 (MQ=255)                                                                                         tGAGGCGGCAGAGACATTCAGTCGCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACt                                                                                                                                                               <  1:301041/250‑1 (MQ=255)                                                                                                     gaCATTCAGTCTCAACAACCGCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCtt                                                                                                                                                  <  2:575287/251‑1 (MQ=255)                                                                                                             gTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGCTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACCCTCGTTTGCTGGCTTTGATGGGc                                                                                                                                          >  1:28355/1‑251 (MQ=255)                                                                                                                ccaacaaCCTCGGACGCGTTGCCGAGAAGCTGCCGTCAGAACCACGGGAAAATAGCTTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTG‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTGTGATGGGC‑GAg                                                                                                                                      <  2:401258/250‑1 (MQ=255)                                                                                                                acgctctgccgatccGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGTCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCGTTGATGGGC‑GAg                                                                                                                                      <  2:263210/237‑1 (MQ=255)                                                                                                                 caacagCCTCGGACGCTTTGCCGATAAGCGGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTGTGCTGGCTTTGATGGGC‑g                                                                                                                                        <  2:74589/248‑1 (MQ=255)                                                                                                                   acaacCTCTGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCACGCAAATTCCAGTGGCGATGACTCTGGTAACGAATATGCCAATCGGTTCGGGCTTAGGCTCCCGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCACGCCGCTTAATGACACTCGTTTGCTGGCTTTTAAGGGC‑GATCTg                                                                                                                                   >  2:396453/1‑251 (MQ=255)                                                                                                                          cGGACGTTTTGCCGTTAAGCTGCCGTCCGAACCACGGGAAAATATCGTTTAGCAGTTCTGGGAGCGTTTTTGCCAGGA‑GTGTGGCAAGCAAAGTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAGCACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAggac                                                                                                                            <  2:631281/251‑3 (MQ=255)                                                                                                                               gCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAg                                                                                                                                      >  1:263210/1‑236 (MQ=255)                                                                                                                               gCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAggacgaatc                                                                                                                       >  1:21446/1‑244 (MQ=255)                                                                                                                                  cacgctcgcccgatccttcaaaaCCACGGGTAAATAGCGTTTATCAGTGCGGGTAGCGTTTTTGCCAGGAGCTT‑GGCAATCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATTACACGCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTcc                                                                                                                    <  2:378012/230‑1 (MQ=255)                                                                                                                                      cGATAAGCTGCCGTCAGATCCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGgca                                                                                                                <  1:475998/251‑1 (MQ=255)                                                                                                                                         tAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGACAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGGTTAATGCCACTCGTTTTCTGGCTTTTATGGGCCGA‑CTGACAGGACGAATCTCCGgcagcc                                                                                                             >  2:452553/1‑250 (MQ=255)                                                                                                                                           aGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGcatt                                                                                                           >  1:287581/1‑251 (MQ=255)                                                                                                                                                  gTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAAtctca                                                                                                                    >  1:378012/1‑234 (MQ=255)                                                                                                                                                  gTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACg                                                                                                    >  1:46403/1‑251 (MQ=255)                                                                                                                                                  gTCAGAACCACGGGAAAATATCGTTTAGCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAAGCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCGGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACg                                                                                                    <  1:618009/251‑1 (MQ=255)                                                                                                                                                         ccACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGt                                                                                             >  1:65022/1‑251 (MQ=255)                                                                                                                                                         ccACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGt                                                                                             <  2:636959/251‑1 (MQ=255)                                                                                                                                                           aCGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATTGGC‑GAGCTGGAAGGACGAATCTCCAGCAGCATTCATTACGACAACGTgg                                                                                           <  1:501287/251‑1 (MQ=255)                                                                                                                                                             gggAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTG‑GGTAAGCAAATTCCAGTGGCGATGACCATGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCa                                                                                         <  1:342319/251‑1 (MQ=255)                                                                                                                                                                  aaTATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCgtg                                                                                    <  1:137465/251‑1 (MQ=255)                                                                                                                                                                    tatCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCCAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCCATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGGACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGCAGGACGAATCTCCGGCAGCCTTCCTTACGACAACGTGGCACCGGGtt                                                                                  >  2:381404/1‑251 (MQ=255)                                                                                                                                                                         tttATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAAt                                                                                                                                                                     <  1:675676/164‑1 (MQ=255)                                                                                                                                                                                  gcggggAGCGTTTGTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATg                                                                    <  2:287581/248‑1 (MQ=255)                                                                                                                                                                                  gCTGGGATCGTTTTTGCCCGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTTGAAAAGAATATGCCAATCGGTTCGGGCGTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGTGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATg                                                                    <  2:21446/251‑1 (MQ=255)                                                                                                                                                                                  gCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAACAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGACTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGacac                                                                                                                                                                >  2:76827/1‑160 (MQ=255)                                                                                                                                                                                  gCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGacac                                                                                                                                                                <  1:76827/160‑1 (MQ=255)                                                                                                                                                                                       ggaggttttgtgcaaggagcttggTAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGATTTAGGCTCCAGCTCCTGTTCGGTGGTCGCGGCTCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGt                                                                <  2:486787/229‑1 (MQ=255)                                                                                                                                                                                           gTTTTTGACAGGAACTT‑GGCAAGCAAATTCCCGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGGTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGAAGAATCTCCGGCAGCATTCATTACGACAGCGTGACACCTGGTTTTCTTGGTGGAATGCAGTtgatg                                                           >  2:615022/1‑251 (MQ=255)                                                                                                                                                                                             ttttGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTtgatga                                                          <  1:591318/250‑1 (MQ=255)                                                                                                                                                                                                  ccAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGATGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCga                                                      >  1:434613/1‑249 (MQ=255)                                                                                                                                                                                                  ccAGGAACTT‑GGAAAGCAACTTCCAGTGGCGATGACTCTGGTAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGCATGAACAATGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTTGCAGGACGAATCTCCGGCAGCATTCATTACGCCAACGTGGCACCTTGTTTTCTTGGAGGTATGCAGTGTATGATCga                                                      >  2:248952/1‑249 (MQ=255)                                                                                                                                                                                                               caaGCAAATGCCAGTGGCGATGACTCTGGAAAATAATATGCCAAGCGGTTCGGGCTTAGGCTCCAGCGCCTGTGCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCGTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGAcatca                                        <  2:453374/250‑1 (MQ=255)                                                                                                                                                                                                                aaGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTGGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGCTGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGAcatcat                                       >  1:566781/1‑251 (MQ=255)                                                                                                                                                                                                                  gCAAATTCCAGTGGCGATGACTCTGGAGAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGCACACTGTGGCCAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGACTCTCCGGCAGCAGTCATTAGGACTACGTGGCACCGTGTTTTCTTGGAGGAATGCAGTTGATGATCGAAGAAAACGAcgacatc                                      >  2:387716/1‑244 (MQ=255)                                                                                                                                                                                                                           aGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCa                              >  1:209199/1‑249 (MQ=255)                                                                                                                                                                                                                             tGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGCCGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGGGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCaaagg                          >  2:137276/1‑248 (MQ=255)                                                                                                                                                                                                                             tGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCaa                             <  1:137276/248‑1 (MQ=255)                                                                                                                                                                                                                              ggCGATGACTCTGGAAAAGACTATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGc                         >  1:487224/1‑251 (MQ=255)                                                                                                                                                                                                                              ggCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGc                         <  1:449166/251‑1 (MQ=255)                                                                                                                                                                                                                                 gATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGAGCTGATGGCGATGAATGAAAACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GCGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAAAGTGGCACCGAGTTTTATTGGTGGTATGCAGTTGATGATCGAAGAAAAAGACATCATCAGCCAGAAAGTGcc                        >  2:496142/1‑249 (MQ=255)                                                                                                                                                                                                                                          ggAAAAGAATATGCCAATCGGGTCGGGCTTAGGCTCCAGCGGCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTtga               <  2:321868/249‑1 (MQ=255)                                                                                                                                                                                                                                           gAAAAGAATGTGCCAATCGGTTCGGGCTTAGGCCCCAGCGCTTTGTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACGCTGTGGCAAGCCTCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GTGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCTAGTGCCAGGGTTtgatga            <  2:434613/251‑1 (MQ=255)                                                                                                                                                                                                                                                     tGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTgg  >  1:611675/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                        CCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTG‑GGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGG  >  NC_000913/2826‑3319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑