Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,388,753	G→A	G40D (GGT→GAT)	yfbO →	uncharacterized protein YfbO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,388,753	0	G	A	100.0%	88.6 / NA	30	G40D (GGT→GAT)	yfbO	uncharacterized protein YfbO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/16);  total (14/16)

ATTCATGTTATGATGCTCTTGAAACTAATAATACGTATGGAATTGTCACTATTTATCTGCTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGA  >  NC_000913/2388519‑2388990                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              aTTCATGTTATGATGCTCTTGAAACTAATAATACGTATGGAATTGTCACTATTTATCTGCTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAAt                                                                                                                                                                                                                               >  1:515138/1‑251 (MQ=255)               gCTCTTGAAACTAATAATACGTATGGAATTGTCACTATTTATCTGCTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACa                                                                                                                                                                                                                 <  1:480385/251‑1 (MQ=255)                    tGAAACTAATAATACGTATGGAATTGTCACTATTTATCTGCTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAAt                                                                                                                                                                                                               <  1:149065/248‑1 (MQ=255)                                            tGTCACTATTTATCTGCTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCAtttt                                                                                                                                                                                     <  1:550771/250‑1 (MQ=255)                                                             tAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCGTTAGAACGCATTTCCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTGGCAATGTGATTCCAGAACAATCTCCGCa                                                                                                                                                                                                        <  2:497318/214‑1 (MQ=255)                                                              aaTGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGt                                                                                                                                                                   <  1:660636/250‑1 (MQ=255)                                                              aaTGAATTAAAACAATGTCATTCGTTTGCTGAATATATTATTTCAGGGAATAATTATGAAACCTTTAGAACGCATTACACAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGTTTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCAATCACCATTTTCTGTAAATACGTGAGCGt                                                                                                                                                                   >  2:67046/1‑250 (MQ=255)                                                                      cAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGTAAATACGTGAGCGTAACAATGtt                                                                                                                                                          <  2:254593/251‑1 (MQ=255)                                                                                cATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGtt                                                                                                                                                 <  1:180625/250‑1 (MQ=255)                                                                                  ttCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTg                                                                                                                                              >  1:180474/1‑251 (MQ=255)                                                                                        tgatgaATATATTATTTCAGGGTATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGa                                                                                                                                         >  2:415235/1‑250 (MQ=255)                                                                                                 tattatTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACATTGTTAGTGATGTTTTGGTAGAAATTACGATg                                                                                                                               >  2:10066/1‑251 (MQ=255)                                                                                                  attattTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGGGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTGGGTAGAAGTTagtatgt                                                                                                                              >  1:380477/1‑245 (MQ=255)                                                                                                    tattTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGTTTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGCTGTTTTGGTAGCAATTACGATGTTc                                                                                                                            >  2:288429/1‑251 (MQ=255)                                                                                                       ttCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATAAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCAGCAAGCTATCTATCACCATTTTCTGAAAATCAGTTAGCGTAACAATGTTAGTGATGTTTTGGTAGTACTTAAGATGTTCGAt                                                                                                                         >  2:252186/1‑251 (MQ=255)                                                                                                            ggAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGAccc                                                                                                                    >  1:364138/1‑251 (MQ=255)                                                                                                                  atcaTGACACCTTTAGACCGCATTACCCAACTTGTAACTATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATACTATCCCTGATTCATTATGTTGCAATGTGATTCCGGAACAATCTCCGCAAGATATCTATCACCATTGTCTGAAACTACAAGAGAGTAACAATGTTATTGATGTTTTGGTAGAAATTACGATGTTCGTTGACCCAGACTg                                                                                                              >  2:257825/4‑251 (MQ=255)                                                                                                                     aTGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTTATACTCCCCGGCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATCATAGCCATGATTCAATATGTTGCAATGTGATTCCAGAACAAGCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCCGACTGGc                                                                                                            <  2:600647/250‑1 (MQ=255)                                                                                                                                  aatgCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTTATACTACTCGTCCAGTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGGCCCAGACTGGCCCTTTAGTGAAAGt                                                                                              <  2:552951/248‑1 (MQ=255)                                                                                                                                       aTTACCCCACTTGTTAATATCAATGGTGATGGTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATACTATCCATGATTCAATATGTTGCAATGTGATTCAAGAACAATCTCAGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAAAAATGTTAGCGATGTTTTGGTAGACATTACGATGTTCGATGACCCAGCCTGGCACTTTAGTGAAAGTAtttt                                                                                         >  2:662466/1‑251 (MQ=255)                                                                                                                                                  tGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACAATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATcac                                                                                <  1:102475/249‑1 (MQ=255)                                                                                                                                                         atCAATGGTGATTTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCtt                                                                        <  2:644728/250‑1 (MQ=255)                                                                                                                                                          tCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTTTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTc                                                                       <  2:515138/250‑1 (MQ=255)                                                                                                                                                           cAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCa                                                                      <  2:510483/250‑1 (MQ=255)                                                                                                                                                                                tgctACTCCTCGTCCATCGTTATCTCTGGAGGTTTCTTTTATTGACAATAATCTCCATGATTAAATATGTGGCAGTGTGATTCGAGAACAATCTCCGCAAGCTATCTATCACCATTTTTTGAGAATACGTGAGCGTAACAATGTTAGCGATGTTTTGGTAGCAATTACGATGCTCGATGACCCAAACTGGCCCTTCAGCGGAAGCATTTTAGTTATCACCAGTGCTTGACCAGACGAGGGTCAGAGCTGGt                                                >  2:129804/4‑251 (MQ=255)                                                                                                                                                                                    aCTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTGCACCAGAAGAAGTTCAGAGCTGGTTTg                                             <  1:455614/250‑1 (MQ=255)                                                                                                                                                                                                 tGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGc                                <  2:180474/250‑1 (MQ=255)                                                                                                                                                                                                       ctctTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCtgat                         >  1:1684/1‑251 (MQ=255)                                                                                                                                                                                                                          tgataataatATCCATGATTCAATATGTTGCAATGGTATTCCAGAACAATCTCCGCACGCTATCTATCACCAGTTTCTGAAAAGACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTAGTTTAGTTATCTCCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATgg       <  2:610516/246‑1 (MQ=255)                                                                                                                                                                                                                                 aataTCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCaga  >  1:339186/1‑248 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              ATTCATGTTATGATGCTCTTGAAACTAATAATACGTATGGAATTGTCACTATTTATCTGCTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGA  >  NC_000913/2388519‑2388990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑