Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,055	C→T	T85T (ACC→ACT)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,055	0	C	T	97.1%	197.9 / ‑2.1	70	T85T (ACC→ACT)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (32/36);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.23e-01

CCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTG‑GGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCC  >  NC_000913/2817‑3297                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    ccctgtcTTCCAGTGCCAATATGAGCGTTGGGTTTGATGGGCTCGGTGAGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCATTCACAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTTCTGGGAGCGTATTTGACAGTAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGTAAAAGaa                                                                                                                                                                                                                                          <  2:40743/244‑1 (MQ=255)  cccGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGAGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAat                                                                                                                                                                                                                                         >  1:389979/1‑250 (MQ=255)       cTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGcc                                                                                                                                                                                                                                    >  1:185418/1‑250 (MQ=255)          ccAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATc                                                                                                                                                                                                                                <  1:412413/251‑1 (MQ=255)          ccAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATc                                                                                                                                                                                                                                <  1:331953/251‑1 (MQ=255)                          cGTCGGGTTTGGTGTGAGCGGGGCGGCGGTGACACCTGTGGATGGTGCATTGCTTGGAGATGTAGTCACGTTTGAGGCGGCAGAGACATTCAGTCTCAAAAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATAGCGTTTATCAGTGCTGGGAGCGTGTTTGCCAGGAGCTG‑GGCAAGCAAATTCCATTGGCGATGACTCTGGAAAAGAATTTGCCAATCGGTTCGGGCTTAGGCt                                                                                                                                                                                                                <  2:34952/251‑1 (MQ=255)                                       gtgCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTc                                                                                                                                                                                                   <  1:538490/251‑1 (MQ=255)                                            aggggCAGCGGAGACACATGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCCAAGACATTCCGTCTCCACAACCTCGGACGCTTTGCCGCTAAGCTGCCGTCAGGACCACGGGAAAATCTCGTTTATCAGTGATGGGCGCGGTTTTGACAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATTCATCTGGAAAAGAATATGCCAATAGGTTCGGGCTTAGACTCCAGCGCCTGTTCggagg                                                                                                                                                                                              >  2:449166/2‑248 (MQ=255)                                                      gTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGAAGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGACAATCGGTTCAGGCTTAGGCTCCAGCGCCTTTTCGGGGGGcgcgtggct                                                                                                                                                                                    >  2:598968/1‑246 (MQ=255)                                                          cacCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATg                                                                                                                                                                                <  1:180342/251‑1 (MQ=255)                                                          cacCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATg                                                                                                                                                                                >  1:74589/1‑251 (MQ=255)                                                            ccTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGc                                                                                                                                                                              <  1:598968/251‑1 (MQ=255)                                                             cTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGa                                                                                                                                                                                                                                                >  1:187712/1‑184 (MQ=255)                                                             cTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGa                                                                                                                                                                                                                                                <  2:187712/184‑1 (MQ=255)                                                                  gATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑TGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTACGGTGGTCGCGGCGCTGATGGCGatgaa                                                                                                                                                                        <  1:406982/251‑1 (MQ=255)                                                                      gggcagtGCTCGGAGATGTAGTCACGGTGGAGGCGGCAGAGTCATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTATTTGCCAGGAGCTT‑GGCAAGCAAATGCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGAGGTCGCGGCGCTGATGGCGAtgaatgaa                                                                                                                                                                    <  2:73643/245‑1 (MQ=255)                                                                        gCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACAGTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTACGGTGGTCGCGGCGCTGATGGCGATGAATGAaca                                                                                                                                                                  <  1:176281/251‑1 (MQ=255)                                                                                cGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGc                                                                                                                                                           >  1:595577/1‑250 (MQ=255)                                                                                 ggagatgacgacACGGTTGAGGCGGCAGAGACATTCAGTCTCAAAAACCTCGGAAGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCATTTATCAGTGCTGGGAGCGTTTTTGCCCGGAGCTT‑GGCAAGCAACTTCCAGTGGCGAGGACTCTGGACAAGAATATGCCAATCGGTTCGGGCTTCGGCTACAGCGCCCGTTCGGTGGTCGCGGGGCTGTGGGGGATGAATGAAAACTgtggaa                                                                                                                                                          >  2:70200/12‑245 (MQ=255)                                                                                      tGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGc                                                                                                                                                    >  1:498765/1‑251 (MQ=255)                                                                                        tAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCtt                                                                                                                                                  >  1:636959/1‑251 (MQ=255)                                                                                        gaGTCACGGTTGAGGCGGCAGAGACATTCAGTCTAACCAACCTCGTAGGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATAGCGTTTATCAGTGCGGGGAGCGTGTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTGCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTgtttcaggtcgctt                                                                                                                                                  <  2:617875/250‑14 (MQ=255)                                                                                         aggcACGGTTGAGGCGGCAGAGAAATTCAGGCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCTGCAGATCCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCtt                                                                                                                                                  <  2:428810/247‑1 (MQ=255)                                                                                         aGTCGCGGTTGAGGCGGCAGAGACCTTCAGTCTCATCAACCTTGGACGCTTTGCCGATAAGCTGCCGTTAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGGTCGGGCTTAGGCTCCAGCGACTGTTCGGTGGTCGCGGCGCTGATGGAGATGAATGAACACTGTGGCAAGCCGCtt                                                                                                                                                  <  2:500776/250‑1 (MQ=255)                                                                                              cGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGac                                                                                                                                            <  1:647014/251‑1 (MQ=255)                                                                                              cGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTG‑GGTAAGCAAATTCCAGTGGCGATGACCATGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGac                                                                                                                                            >  2:342319/1‑251 (MQ=255)                                                                                               ggTTGAGGCGGCCGAAACAATTAGACTCAACAACCTCGGACGCTTTGACGATAAGCAGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTATTTGCAAGGAGCTT‑GGCAAGCAAATTACAGTGGCGATGACTATGGAACAGTATATGCCAATCGGTTCGGGCTTAGGCTCCGGCGCCAGTTAGGTGGTCGCGGCGCTGATGGCGCTGAATGAaccgtgtggcaaagcgggtaatgac                                                                                                                                            >  2:29444/1‑227 (MQ=255)                                                                                               ggTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGacc                                                                                                                                           >  1:453374/1‑250 (MQ=255)                                                                                                  tGAGGCGGCAGAGACATTCAGTCGCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACt                                                                                                                                         <  1:301041/250‑1 (MQ=255)                                                                                                              gaCATTCAGTCTCAACAACCGCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCtt                                                                                                                            <  2:575287/251‑1 (MQ=255)                                                                                                                      gTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGCTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACCCTCGTTTGCTGGCTTTGATGGGc                                                                                                                    >  1:28355/1‑251 (MQ=255)                                                                                                                         ccaacaaCCTCGGACGCGTTGCCGAGAAGCTGCCGTCAGAACCACGGGAAAATAGCTTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTG‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTGTGATGGGC‑GAg                                                                                                                <  2:401258/250‑1 (MQ=255)                                                                                                                         acgctctgccgatccGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGTCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCGTTGATGGGC‑GAg                                                                                                                <  2:263210/237‑1 (MQ=255)                                                                                                                          caacagCCTCGGACGCTTTGCCGATAAGCGGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTGTGCTGGCTTTGATGGGC‑g                                                                                                                  <  2:74589/248‑1 (MQ=255)                                                                                                                            acaacCTCTGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCACGCAAATTCCAGTGGCGATGACTCTGGTAACGAATATGCCAATCGGTTCGGGCTTAGGCTCCCGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCACGCCGCTTAATGACACTCGTTTGCTGGCTTTTAAGGGC‑GATCTg                                                                                                             >  2:396453/1‑251 (MQ=255)                                                                                                                                   cGGACGTTTTGCCGTTAAGCTGCCGTCCGAACCACGGGAAAATATCGTTTAGCAGTTCTGGGAGCGTTTTTGCCAGGA‑GTGTGGCAAGCAAAGTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAGCACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAggac                                                                                                      <  2:631281/251‑3 (MQ=255)                                                                                                                                        gCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAg                                                                                                                >  1:263210/1‑236 (MQ=255)                                                                                                                                        gCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAggacgaatc                                                                                                 >  1:21446/1‑244 (MQ=255)                                                                                                                                           cacgctcgcccgatccttcaaaaCCACGGGTAAATAGCGTTTATCAGTGCGGGTAGCGTTTTTGCCAGGAGCTT‑GGCAATCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATTACACGCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTcc                                                                                              <  2:378012/230‑1 (MQ=255)                                                                                                                                               cGATAAGCTGCCGTCAGATCCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGgca                                                                                          <  1:475998/251‑1 (MQ=255)                                                                                                                                                  tAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGACAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGGTTAATGCCACTCGTTTTCTGGCTTTTATGGGCCGA‑CTGACAGGACGAATCTCCGgcagcc                                                                                       >  2:452553/1‑250 (MQ=255)                                                                                                                                                    aGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGcatt                                                                                     >  1:287581/1‑251 (MQ=255)                                                                                                                                                           gTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAAtctca                                                                                              >  1:378012/1‑234 (MQ=255)                                                                                                                                                           gTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACg                                                                              >  1:46403/1‑251 (MQ=255)                                                                                                                                                           gTCAGAACCACGGGAAAATATCGTTTAGCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAAGCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCGGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACg                                                                              <  1:618009/251‑1 (MQ=255)                                                                                                                                                                  ccACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGt                                                                       <  2:636959/251‑1 (MQ=255)                                                                                                                                                                  ccACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGt                                                                       >  1:65022/1‑251 (MQ=255)                                                                                                                                                                    aCGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATTGGC‑GAGCTGGAAGGACGAATCTCCAGCAGCATTCATTACGACAACGTgg                                                                     <  1:501287/251‑1 (MQ=255)                                                                                                                                                                      gggAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTG‑GGTAAGCAAATTCCAGTGGCGATGACCATGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCa                                                                   <  1:342319/251‑1 (MQ=255)                                                                                                                                                                           aaTATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCgtg                                                              <  1:137465/251‑1 (MQ=255)                                                                                                                                                                             tatCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCCAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCCATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGGACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGCAGGACGAATCTCCGGCAGCCTTCCTTACGACAACGTGGCACCGGGtt                                                            >  2:381404/1‑251 (MQ=255)                                                                                                                                                                                  tttATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAAt                                                                                                                                               <  1:675676/164‑1 (MQ=255)                                                                                                                                                                                           gcggggAGCGTTTGTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATg                                              <  2:287581/248‑1 (MQ=255)                                                                                                                                                                                           gCTGGGATCGTTTTTGCCCGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTTGAAAAGAATATGCCAATCGGTTCGGGCGTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGTGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATg                                              <  2:21446/251‑1 (MQ=255)                                                                                                                                                                                           gCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAACAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGACTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGacac                                                                                                                                          >  2:76827/1‑160 (MQ=255)                                                                                                                                                                                           gCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGacac                                                                                                                                          <  1:76827/160‑1 (MQ=255)                                                                                                                                                                                                ggaggttttgtgcaaggagcttggTAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGATTTAGGCTCCAGCTCCTGTTCGGTGGTCGCGGCTCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGt                                          <  2:486787/229‑1 (MQ=255)                                                                                                                                                                                                    gTTTTTGACAGGAACTT‑GGCAAGCAAATTCCCGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGGTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGAAGAATCTCCGGCAGCATTCATTACGACAGCGTGACACCTGGTTTTCTTGGTGGAATGCAGTtgatg                                     >  2:615022/1‑251 (MQ=255)                                                                                                                                                                                                      ttttGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTtgatga                                    <  1:591318/250‑1 (MQ=255)                                                                                                                                                                                                           ccAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGATGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCga                                >  1:434613/1‑249 (MQ=255)                                                                                                                                                                                                           ccAGGAACTT‑GGAAAGCAACTTCCAGTGGCGATGACTCTGGTAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGCATGAACAATGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTTGCAGGACGAATCTCCGGCAGCATTCATTACGCCAACGTGGCACCTTGTTTTCTTGGAGGTATGCAGTGTATGATCga                                >  2:248952/1‑249 (MQ=255)                                                                                                                                                                                                                        caaGCAAATGCCAGTGGCGATGACTCTGGAAAATAATATGCCAAGCGGTTCGGGCTTAGGCTCCAGCGCCTGTGCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCGTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGAcatca                  <  2:453374/250‑1 (MQ=255)                                                                                                                                                                                                                         aaGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTGGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGCTGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGAcatcat                 >  1:566781/1‑251 (MQ=255)                                                                                                                                                                                                                           gCAAATTCCAGTGGCGATGACTCTGGAGAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGCACACTGTGGCCAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGACTCTCCGGCAGCAGTCATTAGGACTACGTGGCACCGTGTTTTCTTGGAGGAATGCAGTTGATGATCGAAGAAAACGAcgacatc                >  2:387716/1‑244 (MQ=255)                                                                                                                                                                                                                                    aGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCa        >  1:209199/1‑249 (MQ=255)                                                                                                                                                                                                                                      tGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGCCGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGGGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCaaagg    >  2:137276/1‑248 (MQ=255)                                                                                                                                                                                                                                      tGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCaa       <  1:137276/248‑1 (MQ=255)                                                                                                                                                                                                                                       ggCGATGACTCTGGAAAAGACTATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGc   >  1:487224/1‑251 (MQ=255)                                                                                                                                                                                                                                       ggCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGc   <  1:449166/251‑1 (MQ=255)                                                                                                                                                                                                                                          gATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGAGCTGATGGCGATGAATGAAAACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GCGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAAAGTGGCACCGAGTTTTATTGGTGGTATGCAGTTGATGATCGAAGAAAAAGACATCATCAGCCAGAAAGTGcc  >  2:496142/1‑249 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    CCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTG‑GGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCC  >  NC_000913/2817‑3297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑