Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,204,395	T→A	intergenic (+106/‑201)	yehK → / → yehL	uncharacterized protein YehK/putative AAA(+) MoxR family ATPase YehL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,204,395	0	T	A	100.0%	76.3 / NA	26	intergenic (+106/‑201)	yehK/yehL	uncharacterized protein YehK/putative AAA(+) MoxR family ATPase YehL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (17/9);  total (17/9)

ATGATGCGTTGATTTATATAAGAGATGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAAC‑TAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAGGGATGAATATATGTCACCGCAGAATAATCATCTGCAGCG  >  NC_000913/2204162‑2204624                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       atgatgCGTTGATTTATATAAGAGATGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACg                                                                                                                                                                                                                       >  1:251122/1‑251 (MQ=255)        gTTGATTTATATAAGAGATGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGc                                                                                                                                                                                                                >  1:275471/1‑251 (MQ=255)                                   tgatgaGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAAt                                                                                                                                                                                     >  1:330047/1‑251 (MQ=255)                                           ggAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGtaaata                                                                                                                                                                             <  1:493945/251‑1 (MQ=255)                                              aaTTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGtaaataaa                                                                                                                                                                           >  1:417844/1‑250 (MQ=255)                                                        tGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCt                                                                                                                                                                >  1:49481/1‑251 (MQ=255)                                                         gAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAATAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCt                                                                                                                                                                <  1:659149/250‑1 (MQ=255)                                                              aCCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACAAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGAACCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCAAGCCGTGGTTATAAATCGTTTGTTGGAACACGTTGTTGCACAGGCTGTGGGGTTAGGCCTGAAAATAGTAACTAAAAGACCCTGCCTCAcacg                                                                                                                                                          >  2:412819/1‑248 (MQ=255)                                                                    aaCTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGCACCTGCCTCACCAGCAGGtt                                                                                                                                                    >  1:214626/1‑251 (MQ=255)                                                                    aaCTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGtt                                                                                                                                                    <  1:437142/251‑1 (MQ=255)                                                                                                   cAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCACAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGACTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCCGGTTTTTTTATTTACTGGGATCTGCTTTCCAgct                                                                                                                      >  2:160731/1‑248 (MQ=255)                                                                                                   cAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCAGCGCAACGTACCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGGGGTTATAAATCGTTCGTTGTAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACAAGCGGGTTTTTTTATTTACTGTGATCTGCTTTCCAGat                                                                                                                      >  2:526505/1‑250 (MQ=255)                                                                                                                  taaGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGACCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCTGGGTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAaac                                                                                                       >  2:136363/2‑250 (MQ=255)                                                                                                                  cAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTgtggt                                                                                                                                                                                                    <  1:416205/157‑1 (MQ=255)                                                                                                                  cAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTgtggt                                                                                                                                                                                                    >  2:416205/1‑157 (MQ=255)                                                                                                                                       ctAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAAC‑TAATGCGCCAAACAttt                                                                                  <  1:446001/249‑1 (MQ=255)                                                                                                                                                    aTTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTACCTCACCAGCAGGTTTTTTTATTTACTTAGATCTGCTTTCCAGATATTTTTCGCTCAAACAAC‑TAATTCGCCAAACATTTATTGACCGTAAAatt                                                                   >  2:596054/1‑250 (MQ=255)                                                                                                                                                         tccgataaggCGCAACGTGCAAATGGCCTGGATTATATATCTCATTATCTTAATTGCAACGGGGTCCAGACGTGGTTATAAATCGTTCGTTGGAAAACGATGATGCACGGGCTACGGCGTTAGGCCTGAAAATAGTAAATAAAAGACCCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATATGCTTTCCGTCAATTTTTCGTTCAA‑CAACCTAATGCGCACAACATTTAATGCGCGTAAATTATCGtt                                                              >  2:193178/9‑251 (MQ=255)                                                                                                                                                           cGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTc                                                                                                                                                               >  1:246827/1‑153 (MQ=255)                                                                                                                                                           cGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTc                                                                                                                                                               <  2:246827/153‑1 (MQ=255)                                                                                                                                                                         gTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCACCGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAAC‑TAATGCGCCATACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACAt                                              >  1:591722/1‑251 (MQ=255)                                                                                                                                                                                            aaaacctcATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGGTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAAC‑TAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATGTCATTAATACATTTCAGGGATGAATATATGt                           <  2:337942/246‑1 (MQ=255)                                                                                                                                                                                                        tCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTTCCTCACcag                                                                                                                                                          <  2:391849/113‑1 (MQ=255)                                                                                                                                                                                                        tCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACcag                                                                                                                                                          >  1:391849/1‑113 (MQ=255)                                                                                                                                                                                                            aaTTGCAACGGGGGCCAGCCGTGGTTATAAATCGTTCGTGGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAAC‑TAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAGGGATGAATATATGTCACCGCAGAATAatca           <  2:443692/251‑1 (MQ=255)                                                                                                                                                                                                                     ggggTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGATGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAAC‑TAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAGGGATGAATATATGTCACAGCAGAATAATCATCTGCAGCg  >  1:282579/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       ATGATGCGTTGATTTATATAAGAGATGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAAC‑TAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAGGGATGAATATATGTCACCGCAGAATAATCATCTGCAGCG  >  NC_000913/2204162‑2204624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑