Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,894,839	T→C	L12P (CTC→CCC)	pabB →	aminodeoxychorismate synthase subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,894,839	0	T	C	100.0%	139.9 / NA	43	L12P (CTC→CCC)	pabB	aminodeoxychorismate synthase subunit 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/17);  total (26/17)

GGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTGGATCGCGCAGACAT  >  NC_000913/1894599‑1895079                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 ggTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCa                                                                                                                                                                                                                                         <  1:681320/250‑1 (MQ=255)                         ccaGGGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTcc                                                                                                                                                                                                                <  2:215926/251‑1 (MQ=255)                              gcgATTGCCTGGCCGCTGCTCATCCCTTGGGCCATCAGGTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGAAAACATTGTTAACTCCTGCTAAATTGTTGGCGATAATTATTTCATGCTACCCGGCACATAGCCAGTAGATTC‑AGAACTGGTGAAGAAGTTATTTCCCGCTGTGCTTACTTTACCCTGGCGTCAGACCGCCGCTAACTTTTCTTTCTCCCGCtt                                                                                                                                                                                                           >  2:550379/1‑251 (MQ=255)                                          ccGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACGCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGcc                                                                                                                                                                                               <  2:263939/251‑1 (MQ=255)                                                cccATCCCTTGGGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCGGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGc                                                                                                                                                                                         <  2:549665/249‑1 (MQ=255)                                                         tgtgCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGCCGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCtttt                                                                                                                                                                                >  1:143016/1‑251 (MQ=255)                                                                       cTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTACTGCTCAATTGTTGGCGCTAATTATTTCATGCTACACGGAACATAACAAGCAGAGTC‑AGGACTGATGAAGTCGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGCCGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATg                                                                                                                                                                  >  1:624938/1‑251 (MQ=255)                                                                           aTCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGAC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCg                                                                                                                                                               >  1:516707/1‑250 (MQ=255)                                                                                cTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAAttt                                                                                                                                                                                                                         <  2:486697/187‑1 (MQ=255)                                                                                cTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAAttt                                                                                                                                                                                                                         >  1:486697/1‑187 (MQ=255)                                                                                        cTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGc                                                                                                                                                 >  1:625938/1‑251 (MQ=255)                                                                                          ttttGCTGCTTTGCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTCCCCGGCACATAGCCATTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGTTGAATTTTAGTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGcc                                                                                                                                                <  2:175740/250‑1 (MQ=255)                                                                                             tgctgcTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCtt                                                                                                                                            >  1:59367/1‑251 (MQ=255)                                                                                                       cATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATtgt                                                                                                                                   <  1:221507/250‑1 (MQ=255)                                                                                                             tgagtgaAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCgcc                                                                                                                            >  1:550283/1‑251 (MQ=255)                                                                                                                gtgaAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATGGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAGGACGTTATCTCCCGCTGTGATTACTTTGCCCTGGCGTCAGGACGCCGCTGAATTTTATTTCCCCCGCTTAAGCCCCCTGCCGTGGGCGATGCTTTTACTCTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCgccgag                                                                                                                         <  1:318918/251‑1 (MQ=255)                                                                                                                  gaAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGCTTACTTTACCCTGCCGTCCGGACGCCGCTGCGTTTTATTTCTCCCGCTTAAGCCACCAGCCGTGGGCGATGCTTTTACCCTCCGGCTATGCCCATCATCCGTATAGCCGCTTTGATATTTTGGTCgccgaccc                                                                                                                       >  2:155110/1‑248 (MQ=255)                                                                                                                               cacaCATTGTTAACTACGGCTAGATTGTTGTCGCTAATTATTTCATCCTACCCGGCACCTAGACAGAAGCGCC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGAGTCAGGACGCCGCTGAATTTTATTTCTCCAGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGTCCATTCCGTTCATCCGTAAATCCGCTTTGATATTGTGGTCGCCGAGACGATTTGccattt                                                                                                           >  2:406457/4‑245 (MQ=255)                                                                                                                               cAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACttt                                                                                                           >  2:666474/1‑250 (MQ=255)                                                                                                                                 aaCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAAc                                                                                                        >  1:168693/1‑251 (MQ=255)                                                                                                                                          aaacccTGATAAATTGTTGGCTCTAATTTTTTCATGCTACCCGGCCCATAGCCATGAGAGTC‑AGGACTGATGAAGACGTTATCCCCCGCTGTGATTACTTTTCCCTGGCTTCAGGACGCCTCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGAGGCTTTTACACTCCGGCTATGCCGATCAGCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACGTTAACCTCTTTCgg                                                                                               <  2:617334/247‑1 (MQ=255)                                                                                                                                                     aaTTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTAGGTAAAGAAACCg                                                                                    >  1:615211/1‑251 (MQ=255)                                                                                                                                                             gcgttAATTATTTCATGCTACCCTGCACAGAGCCAGTAGAGTC‑AGGACTGAGGAAGACGTTATCTCCCGCTGTGATTACTTTACCCGGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGTGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGtat                                                                                                                                                    <  2:90647/175‑1 (MQ=255)                                                                                                                                                             gcgcTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGtat                                                                                                                                                    >  1:90647/1‑179 (MQ=255)                                                                                                                                                                 tAATTGTTTCACGCTACCCGGCACAAAGCGAGTATA‑TCTAGGATGGATAAAGACTTAATCCCCCTCTATTATTACTTTACCCGGGCGTTGGGCCTCCGCTATATTTTATTTCTCCCGTGTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTAGGACGTTCATCCGTATAGCCGCTTGGATATGGTGTTCGCAGAGCCGATTTGCACGTTAACCACTTTCGGGAAAGAAACCGCTGTGAGTGaa                                                                         <  2:111426/250‑1 (MQ=255)                                                                                                                                                                  aaTTATTTCATTCTACCCGCCACATAGCCTGTAGATTC‑AGGACTGATTAACACGTTATCTCCCGCTGTGATTTCTTTACCCTGGCGTCAGGACGCCTCTGATTTTTATTTCTCCCGCTTATGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCCCTTTCGGTAAAGAAACCGTTGTTAGTGaa                                                                         <  1:468982/249‑1 (MQ=255)                                                                                                                                                                     tattTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTCAAGAAACCGTTGTTAGTGAAAg                                                                       >  2:484702/1‑248 (MQ=255)                                                                                                                                                                          cATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAAc                                                               >  1:337825/1‑251 (MQ=255)                                                                                                                                                                           aTGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGCAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCAACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTc                                                                                                 >  2:321002/1‑216 (MQ=255)                                                                                                                                                                           aTGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCGTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTc                                                                                                 <  1:321002/216‑1 (MQ=255)                                                                                                                                                                           aTGCTACCCGGCAAATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTGCCCCGGGCGCCAGGACGCCGCTGAATTTTATTTCCCCCGCTTAAGCCCCCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACg                                                              <  1:484702/251‑1 (MQ=255)                                                                                                                                                                                 cccGGCACATAGCCAGTAGAGTC‑AGGACTGATGACGCCGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGCCGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTCCACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAACACGCACAAc                                                        >  2:338823/1‑251 (MQ=255)                                                                                                                                                                                  ccGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACg                                                       >  1:302450/1‑251 (MQ=255)                                                                                                                                                                                     gCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGAcc                                                    <  1:217713/251‑1 (MQ=255)                                                                                                                                                                                         aTAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTCACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACtg                                                >  2:85225/1‑251 (MQ=255)                                                                                                                                                                                           aGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACtgat                                              >  1:218114/1‑251 (MQ=255)                                                                                                                                                                                                         aGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCg                                                                     >  1:207675/1‑215 (MQ=255)                                                                                                                                                                                                           gACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTg                               <  1:184968/251‑1 (MQ=255)                                                                                                                                                                                                                 tgaAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTcc                           >  1:466103/1‑249 (MQ=255)                                                                                                                                                                                                                     gACGTTATCTCCCGCTGTGATTACTTCCCCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAgg                     <  1:85225/251‑1 (MQ=255)                                                                                                                                                                                                                         ttATCTCCCGCCGTGATTACTTTACCCTGGCGTCCAGACGCCGCTGAATTTTCTTTCTCCCGCTTAAGCCAACTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGCTCATCCGTATAGCCGCTTTGATATTATGGTCGCCGAGCCGCTTTGCGCTTTCACCACTTTCGGTAAAGAAACCGTTGTTAGAGCAAGCGAAAAACGCACCACGACCACTGATGACCAGCCACAAGGGCTACAGCAGGTgac                 >  2:329396/1‑249 (MQ=255)                                                                                                                                                                                                                                    tgtgATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGTTGCTCCAGCAGGCGCTGGATCgcgc        >  2:111296/1‑249 (MQ=255)                                                                                                                                                                                                                                        aTTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTGGATCGCGCAGACAt  >  1:490599/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 GGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTC‑AGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTGGATCGCGCAGACAT  >  NC_000913/1894599‑1895079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑