Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,667,460	G→A	Q369* (CAG→TAG)	mlc ←	DNA‑binding transcriptional repressor Mlc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,667,460	0	G	A	95.1%	119.9 / ‑3.4	42	Q369* (CAG→TAG)	mlc	DNA‑binding transcriptional repressor Mlc
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (21/18);  total (23/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

CAGCATCACTAACTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGG  >  NC_000913/1667214‑1667678                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                           cAGCATCACTAACTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGATCCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACta                                                                                                                                                                                                                        <  1:358697/251‑1 (MQ=255) cAGCATCACTAACTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCAATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACt                                                                                                                                                                                                                         >  2:597579/1‑250 (MQ=255)           aaCTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCTTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGaa                                                                                                                                                                                                              <  1:657508/251‑1 (MQ=255)           aaCTCCAACGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGa                                                                                                                                                                                                               <  2:228571/250‑1 (MQ=255)                         gCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACgg                                                                                                                                                                                                <  1:552695/251‑1 (MQ=255)                             cacaAATATAAACCAGGAAAATAATTAACCTTGATAGTCTAAGTTATGATTTCATGGCCCACATTGAGATAGCGCAACTTTTGGTAGAACAGTTAAAAAATGTTAAACCTGCAAAAGACGCATCAACACAAAAACGTGATACATCGAGTCTTTTACCAATGCAGCGCCTGCCATCGAGCCCTGGTTAGAAAACTCAGTAATCTCACCGCTGATGGGCTCAAGATACGCAGGACGGGCCTGATGACGGATGc                                                                                                                                                                                            >  1:634080/1‑251 (MQ=255)                                     taAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCctgct                                                                                                                                                                                                     <  1:136387/234‑1 (MQ=255)                                     taAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCctgct                                                                                                                                                                                                     >  2:136387/1‑234 (MQ=255)                                     taAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTg                                                                                                                                                                                      >  1:530022/1‑249 (MQ=255)                                           aGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGAcc                                                                                                                                                                              >  1:211898/1‑251 (MQ=255)                                           aGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGAcc                                                                                                                                                                              >  1:265147/1‑251 (MQ=255)                                              aaaaTAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGCCCggg                                                                                                                                                                           >  1:215487/1‑251 (MQ=255)                                                     ttAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAgg                                                                                                                                                                     >  1:244576/1‑250 (MQ=255)                                                             gAAAGTCTAAGGTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGcc                                                                                                                                                            <  2:414658/251‑1 (MQ=255)                                                                             cTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTACAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGAGCCTGCTGACGGATGCTGTCTGAGATGACCGGGCAGAGGATATCGGCCGCTTTACTTAACGGTg                                                                                                                                            >  2:341403/1‑251 (MQ=255)                                                                              tttCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGATTTACTTAACGGTgg                                                                                                                                           >  1:372748/1‑250 (MQ=255)                                                                               ttCCTGGCCAAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGCAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCCGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTg                                                                                                                                            >  2:86823/1‑249 (MQ=255)                                                                                      cccAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCa                                                                                                                                   <  1:511775/251‑1 (MQ=255)                                                                                               agaTAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAtttttt                                                                                                                          <  1:288833/251‑1 (MQ=255)                                                                                                   aGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTTCTCTCAACGCTGATGGGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTgg                                                                                                                      <  2:530022/251‑1 (MQ=255)                                                                                                    gcgcAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTGTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGTTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTggt                                                                                                                     <  1:217831/251‑2 (MQ=255)                                                                                                          aTTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACTAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaat                                                                                                               <  1:41471/251‑1 (MQ=255)                                                                                                              tGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGatat                                                                                                                                                                 >  1:191150/1‑197 (MQ=255)                                                                                                              tGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGatat                                                                                                                                                                 <  2:191150/197‑1 (MQ=255)                                                                                                              tGGTAGAACAGTTAAAAAATGGTAAACCTGCAACAGACGAATCAACAAAGACCCGTTATACAGCGCGGCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGGCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaataaa                                                                                                            <  1:25943/250‑1 (MQ=255)                                                                                                                          tAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGACGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTTTGGGTTAAATAAATTCACCATGATgg                                                                                               >  2:553833/1‑251 (MQ=255)                                                                                                                                    tAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATgc                                                                                      <  1:86823/250‑1 (MQ=255)                                                                                                                                     aaCCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATgcg                                                                                     <  1:315997/250‑1 (MQ=255)                                                                                                                                            cAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACAt                                                                             <  1:366351/251‑1 (MQ=255)                                                                                                                                              aCAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATgc                                                                           >  1:119224/1‑251 (MQ=255)                                                                                                                                                 gACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATgcgcg                                                                        >  1:484333/1‑251 (MQ=255)                                                                                                                                                    gAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCACTGGTTAGAAAACTGAGTACTCTAAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGCAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGGATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACGTGAGCGccc                                                                     >  2:521528/1‑251 (MQ=255)                                                                                                                                                             aaGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGAGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGACGAGGATATCTGCCGCTTTACTTAACGGTGAGCCACTCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCAccacgttc                                                            >  2:406472/1‑245 (MQ=255)                                                                                                                                                                  tcGTTATACATCGCGTATTTTACCAGTGCAGCGACTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGAGCCTGCTGACGGATGCTGTCTGAGATGAACGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCAAATCCGAATTATTTGTGGGTTAAATAAATTCACCATGATGGAAATAATGCGACCGACATGCGCGCCAACCCCGGTAatgat                                                       >  2:217834/2‑251 (MQ=255)                                                                                                                                                                       ataCATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCtt                                                  >  1:273115/1‑251 (MQ=255)                                                                                                                                                                       ataCATAGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTAGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGATGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCACTCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCCAGAATGCTCCCGACATGCGCCACCACCCCGGTAATGATGGCtt                                                  >  2:630397/1‑251 (MQ=255)                                                                                                                                                                                                         aTCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATTGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGaca                <  1:597579/251‑1 (MQ=255)                                                                                                                                                                                                               cccTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATg           >  1:236697/1‑250 (MQ=255)                                                                                                                                                                                                                ccTGGGTAGAAAACTGATTATTAGCAACTCTGATGTGCGGATGATAAGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTAATTAACGGTGAGCCAAGCAGTATTTTTTTTGGGTTAAATAAATTCCCCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTTGATCGCTGCGCAATGCCGCGTGACGCAATGAg         <  2:325743/251‑1 (MQ=255)                                                                                                                                                                                                                 cTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAAGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGATAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCAACCCCGGCAATGATGTCTTTTGCCAGTCGATCTCCGCGCAATGCCGCCTGACACAAGGAGt        >  2:360181/1‑251 (MQ=255)                                                                                                                                                                                                                   ggTTAGAAAACTGAGTACTCACAACGCGGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTc       <  2:265147/250‑1 (MQ=255)                                                                                                                                                                                                                       aGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGAAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCCCgg  >  1:439472/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                           CAGCATCACTAACTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGG  >  NC_000913/1667214‑1667678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑