| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,652,331 | T→C | intergenic (+2346/+397) | ydfD → / ← ynfP | lysis protein/protein YnfP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,652,331 | 0 | T | C | 96.4% | 79.4 / ‑5.8 | 28 | intergenic (+2346/+397) | ydfD/ynfP | lysis protein/protein YnfP |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (14/13); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
GTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGG‑TCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGC‑AC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGC > NC_000913/1652092‑1652557 | gtttttttGTTTATACTGGAGTGCGCCCCGGTGAGATGTGGTCACGTGGATTGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGG‑GTATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTAt < 2:521493/244‑1 (MQ=255) tGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAATCGGG‑GCATTCATCTTATTAAGCCAGCAATCGAAGCTCTCCGGAGGCAG‑ATGACATTAAAGAGACTGAGCAAAGAGAAAATCATTGTTG‑TTCACCTCCGAGAGGATGGcaga > 1:11584/1‑250 (MQ=255) ggAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGG‑TCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGaaaaa < 1:208548/249‑1 (MQ=255) cGCCCTTGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGTAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGG‑TCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAAt < 1:518725/250‑1 (MQ=255) ggTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGG‑TCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGC‑AC‑Ctt < 1:424977/251‑1 (MQ=255) gggAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAGAAGACCGTTTCACGGTACCAAAAACACAGGCGGTAAGAAACAGGG‑GCATACATCTTATTTAGCCAGCAAACACCACCATCCGGCGTCAGGATGACAT‑AAAGAGACTTAGCAAAGAACATATCATTTGTGATACACTCCA‑CGAGTAAGGCAGAAAAGAAAAACAAAAAAGCCAC‑ATTT‑TTTTTCAACCttcatggta > 2:567364/1‑242 (MQ=255) aaTGGAACAATGATGATCATGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGGCCATTC‑TCTTAATAAGCAAACAATCGGCGCCCTCCGGGGGAAG‑ATGACATTAACGGGACTGGGGGAAACGGATATCAATGGTG‑TTCACCTCAGGGAGGATGGGAGAAAAGAAAAACAAAAATGG‑CC‑CTTTGTTTTTTAACCCGAAGTGTCAGAGAGGGGAAAAAAtttatg > 1:306307/1‑247 (MQ=255) cAGGATAAATTTAGCAAAAGACCTGTTCACTGTACCAAAAACACAGGTGGGAACCATTCTGG‑TCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGCATGGCAGAACAGAAAAACAAAAATGC‑AC‑CGTTGTTTTTCAACCTGAAGTTGCAGCGAGAGTACAAAATTATGGTGACCAGGTTACCGTTg < 2:591149/251‑1 (MQ=255) cAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCCAACGGG‑GCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAG‑ATGACATTAAAGAGACTGAGCAAAAAGCATATCATTGATG‑TTAAACTCCGAGAATAAGGCAGAAAAGCAAAACAAAAATG‑‑ACACTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGAAAAAAATTATGGGGACCATTTTACCggtg > 1:657799/1‑248 (MQ=255) aTTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGG‑TCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGC‑AC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACt < 2:417371/246‑1 (MQ=255) aTTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGG‑GCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGC‑AC‑CTTTGTTTTTCAACCTGAAGGGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACt > 1:417371/1‑246 (MQ=255) aCCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGG‑TCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCCCCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGC‑AC‑CTTTGtt < 2:494911/176‑1 (MQ=255) cACGGTACCAAAAACACAGGCGGGAACCAATCGGG‑TCATTCATCTTATTAAGCCAGCAATCGACGCGCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCGCAGAGAGTATGGCAGAACAGAAAAACAAAAATGC‑AC‑CTTTGTTTTTCAACCTGAAGTGTCAGCgaga < 2:438759/193‑1 (MQ=255) cACGGTACCAAAAACACAGGCGGGAACCAATCGGG‑GCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGGATGGCAGAACAGAAAAAAAAAAATGC‑AC‑CTTTGTTTTTCAACCTGAAGTGTCAGCgaga > 1:438759/1‑193 (MQ=255) cGGG‑TCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGAATGGCAGAACAGAAAAACAAAAATGC‑AC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGCGACCTTTTTACCGTTGACTAAATAAGGCAGATGTGGGACTAAGCGATAAAACGTGCCGGACTCCGACATCGaa > 2:15053/1‑250 (MQ=255) gg‑TCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGC‑AC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAAt > 1:146623/1‑251 (MQ=255) g‑TCATTCATCTTAATAAGCCAGCAATCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGC‑AC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTTCCGGACTCCGCCATCGAAAATc < 2:84725/251‑1 (MQ=255) attcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCTGAGAGTAt < 2:378190/99‑1 (MQ=255) attcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTAt > 1:378190/1‑99 (MQ=255) tattaAGCCTGCCATCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTTAGCAAAGAGCATTACATTGATG‑TTCCCCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGC‑GC‑CTTTGTGTTTCACCCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGTCCATTTTACCGTTGACTCAATAAGGCAGATGTGGTACGCAGCGATAAAACGTGCCGGACTCCGCTATCTAAAATCATATCAGt < 2:601091/248‑1 (MQ=255) tattaAGCCAGCAATCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGC‑AC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGAGTCCGCCATCGAAAATCATATAAGTCg > 2:460601/1‑250 (MQ=255) aaTCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGC‑AC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATCTCAGTAGAGAACTACTTATg > 2:658967/1‑251 (MQ=255) aaTCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGC‑AC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATg > 1:264907/1‑251 (MQ=255) g‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGC‑AC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACa < 1:583614/222‑1 (MQ=255) gAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGC‑AC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCAttt < 1:658967/250‑1 (MQ=255) cAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGC‑AC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTtat > 1:593737/1‑249 (MQ=255) aaaGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGC‑AC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAACTCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGc > 1:231691/1‑251 (MQ=255) aaaGAGAATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGC‑AC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATAAGGGACGAAGCGATAAAACGTGCCGGACTCCGCCCTCACAAATCCTATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGCCACCTGGTGCTAACCCGGCCGTTATAGc > 2:221643/1‑251 (MQ=255) | GTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGG‑TCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAG‑ATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGC‑AC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGC > NC_000913/1652092‑1652557 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |