Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,643,679	A→T	L209Q (CTG→CAG)	ydfU ←	protein YdfU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,643,679	0	A	T	100.0%	142.3 / NA	46	L209Q (CTG→CAG)	ydfU	protein YdfU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (24/22);  total (24/22)

AGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCA‑TGCTGTCAGATTTTGTTGAGCAAGCAGCTCCAGTGATTCCGATGTCTGG  >  NC_000913/1643439‑1643924                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       aGATGATGCGGATCGTCTGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTATCCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCTGCGTCATGTAAGATTCCGGTGACTCTGGATCAACTGTGATGCGTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCgg                                                                                                                                                                                                                                              <  2:490010/251‑1 (MQ=255)          ggATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTc                                                                                                                                                                                                                                     <  1:561176/251‑1 (MQ=255)                 cgGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTcg                                                                                                                                                                                                                              <  1:102815/251‑1 (MQ=255)                    cTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCgg                                                                                                                                                                                                                           >  1:129301/1‑251 (MQ=255)                     tGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGctct                                                                                                                                                                                                                                                   >  1:606043/1‑226 (MQ=255)                     tGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGGTGTGGCTTTCGCGGt                                                                                                                                                                                                                          >  2:543925/1‑251 (MQ=255)                        cATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTaca                                                                                                                                                                                                                       <  1:130267/251‑1 (MQ=255)                             cGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAg                                                                                                                                                                                                                  <  2:264445/251‑1 (MQ=255)                                           ggCTGTGTCTTAACCCATCGTGTGTATTTCTCCTTAGCCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTTGAGCAACGGTGATGCTTACCACCGTCTTTTCCTGGGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTGTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCGTTCGCGGTACACCGAGCAGATTTTTTCCGc                                                                                                                                                                                                    <  2:444588/251‑1 (MQ=255)                                                       aCCCATCGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAg                                                                                                                                                                                        <  2:176414/251‑1 (MQ=255)                                                        cccAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGc                                                                                                                                                                                       >  1:653375/1‑251 (MQ=255)                                                         ccAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCAACGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGGACGATGGCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCg                                                                                                                                                                                      >  2:155327/1‑251 (MQ=255)                                                                    aTTTCGCCTTAACCCAGCCGCGACGTTTAGGCCGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGAt                                                                                                                                                                           <  2:64301/251‑1 (MQ=255)                                                                          ccTTAACCCAGCGGCGACGTTTAGGCAGCTTCATTAAAGATTCCGTAGACTCTGGATCAACGGTGATGCTTACCCCCGTCTTTTCCTGTGGTGATTTATGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCTATACa                                                                                                                                                                        <  2:533994/248‑1 (MQ=255)                                                                               tttacgatcgcaCGTTTAGGCAGCTTCATGAAAGATTCCGGATACTTTGGGTCAATGGTGATGCTTACCACCGGCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGAGTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGTTTTCGCGGTACACCGAGCAGATGTTTCCCGCTGGTAATCCCTGCGAACGACGCGCTACAGTCTCa                                                                                                                                                                  <  2:96679/238‑1 (MQ=255)                                                                                cccAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTACGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGCGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGAGATACAGCCTCAGGt                                                                                                                                                               >  1:243338/1‑251 (MQ=255)                                                                                      ggcgACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGc                                                                                                                                                          >  1:548951/1‑250 (MQ=255)                                                                                         gACGGTTAGTCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCGTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCCACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGGCGCTTTCGCGGTACACCGCGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCa                                                                                                                                                                  >  1:96679/1‑239 (MQ=255)                                                                                               tAGGCAGCTTCATGAAAGTTTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGCCGCGATACAGCCTCAGGAAGTGCATCCaccacc                                                                                                                                                >  1:409392/1‑251 (MQ=255)                                                                                                                 aTTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGGGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACCGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCccac                                                                                                                              >  2:343105/1‑251 (MQ=255)                                                                                                                        agaCTCTGGATCAACGGTGAGGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACTAGGATa                                                                                                                       <  2:618529/251‑1 (MQ=255)                                                                                                                          aCTCTGGATCAACGGGGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAAt                                                                                                                     <  2:182248/251‑1 (MQ=255)                                                                                                                               ggATCAACGGTGATGCTTACCACCTTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTCCCCCGAGCAGATTTTTTCCTCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAg                                                                                                                 <  1:541231/250‑1 (MQ=255)                                                                                                                               ggATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCa                                                                                                                  <  1:62560/249‑1 (MQ=255)                                                                                                                               ggATCAACGGTGATGCTTACCACAGTCTTTTCCTGTGGTGCTTTTTGTTGCTGGTGGGCGTAAGGCCACGTTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTACCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGACAGCAGAGATACAGCCTCAGGTAGTGCATCAACCACCTGCTTGCAGACAGCCCCCCCGGATAATCCAGc                                                                                                                >  2:234089/1‑251 (MQ=255)                                                                                                                                  tCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGAGATTTATGTAGCTGGTGGGCGTACGGCAACGGTACAAGATTTTTTGTGCGTTGTTTCAATATGATGGTGGCGGTCTGCTCTCCCGGTACGCTGTCGCTTTCGCGGTACCCCGAGCAAATTTTTTCCGCTGGTAATCCCAGCGACCGACGCGATACAGCCTCAGATAGTGCATCCCACACCTGCGTGCAGACCCCCCACCAAGATAACTCAGCCa                                                                                                              >  2:671982/1‑250 (MQ=255)                                                                                                                                      cGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCa                                                                                                                                                                                         >  2:581887/1‑171 (MQ=255)                                                                                                                                      cGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCa                                                                                                                                                                                         <  1:581887/171‑1 (MQ=255)                                                                                                                                      acggggatgcgtcctccGTCTTTTCCTGTTGTGATTTTTGTGGCTGGTGGGCTTAAGGCAACGGTGCAAGATTTTTTGGGCGTTGTTTCAAGATGCGTGTTGCTGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTTCACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCCCCTGATTGCAGACCGCCCCCCAGGAGAATTCAGCCAAAGAt                                                                                                         <  2:555376/236‑1 (MQ=255)                                                                                                                                             gcttagcatctgcTTTTCCTGTGGTTATGTTTGGTGCTGGGGGGCGTAAGGTAGAGGTCCAAGACTTTTTGTGCGTGGTTTCAAGATGCTGGTGGCGGTCTGCTCTCCCGGTTCGATGTCGCTGTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACTACGCGATCGAGCTTCAGGTAGTGCAACCACCAGCTGATTGCAGACCGCACACCATGATAATTCAGCCACAGATGATTccc                                                                                                  <  2:208741/239‑1 (MQ=255)                                                                                                                                             gCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCCGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCCCCAGGCTAATTCAGCCAAAGATAATTccc                                                                                                  >  2:528008/1‑251 (MQ=255)                                                                                                                                                                    gtgATTTTTTTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCg                                                                           <  2:295542/251‑1 (MQ=255)                                                                                                                                                                       aTTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTg                                                                         >  1:406633/1‑250 (MQ=255)                                                                                                                                                                          tttGTTGGTGGTGGGCGTAAGGCAACGGTGCAAGATGTTTTGTGCGTTGTTTCAATAGGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTGCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACg                                                                      <  2:267241/250‑1 (MQ=255)                                                                                                                                                                                    gtggGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCa                                                            >  1:399384/1‑250 (MQ=255)                                                                                                                                                                                             aGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCA‑Tg                                                 >  1:219480/1‑251 (MQ=255)                                                                                                                                                                                                aaccGGTGCAAGATTTTTTGTGCGTTGTTCCAATATGCTGGTGGCGGTCTGCTTTCCCTGTGCGTGGTCGCTTTCTCGGTACACCGAGCAGATGTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCCCCCA‑TGCTg                                              <  2:536684/248‑1 (MQ=255)                                                                                                                                                                                                       gCAAGCTTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCCGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGCCGTCAATCACCCA‑GGTTGTCAGAtt                                       >  2:39322/1‑251 (MQ=255)                                                                                                                                                                                                           gATTTTTTGTGCGTTGGTTCAATATGCTGGTGGCGTTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCTCCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCA‑TGCTGTCAGATTttgt                                   <  1:83824/251‑1 (MQ=255)                                                                                                                                                                                                              tttttGTGCGTTGTTTCAATATGCTGGTGGCGGACTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGCGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGCTACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCACACCAGGCTAATTCAGCCACAGATAATTCCCGCTCCTGCGTACCGCTTATTTCGTGCCGGAGAACGTCAATC‑CCCACTGAAGTCAGATTttgttgt                                >  2:655857/1‑250 (MQ=255)                                                                                                                                                                                                                tttGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGTTTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCACTTATTGCGTGACGGATGACTTCAATCACCCA‑TGCTGTCAGATTttgttgggc                              >  2:124014/1‑248 (MQ=255)                                                                                                                                                                                                                           tttCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAAt                                                                                                      <  1:454758/169‑1 (MQ=255)                                                                                                                                                                                                                           tttCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGAGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGACTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCCCCAGGATAATTCAGCCAAAGATAAt                                                                                                      >  2:454758/1‑169 (MQ=255)                                                                                                                                                                                                                                  atGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCA‑TGCTGTCAGATTTTGTTGAGCAAGCAGCTCCAGTGATTc            >  1:180999/1‑251 (MQ=255)                                                                                                                                                                                                                                     cTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCA‑TGCTGTCAGATTTTGTTGAGCAAGCAGCTCCAGTGATTCCgc         >  1:1966/1‑250 (MQ=255)                                                                                                                                                                                                                                            cGGTCTGCTCTCCCGGAACGATGTCGCTTTCGCGGTACACCGCGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGCCGGATGACGTCAATCCCCCA‑TGCTGTCAGATTTTGTTGAGCCAGCAGCTCCCGTGCTTCCGATGACTgg  >  2:489320/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       AGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCA‑TGCTGTCAGATTTTGTTGAGCAAGCAGCTCCAGTGATTCCGATGTCTGG  >  NC_000913/1643439‑1643924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑