Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,405,758 G→A intergenic (‑7/‑221) mcaS ← / → smrA small regulatory RNA McaS/DNA endonuclease SmrA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,405,7580GA96.8% 90.3 / ‑4.1 31intergenic (‑7/‑221)mcaS/smrAsmall regulatory RNA McaS/DNA endonuclease SmrA
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (17/13);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

CGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTC‑TGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTT  >  NC_000913/1405525‑1406004
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      
cGGTTAGCTAATTTTTACCGCAATCGTCCTATTATTTGTTTCAGAGGAGTGATAGTCGCCATACACCCTTGTCCTCGTCAGTAATCTGGCAATTCCGTCGAGAATGCTGCTAACTGCAAAGTTAAAACTGCATAAAAAAAAAGATTC‑TGTCGTCATCCGCCATACACTACATTACTCACAGCATTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCAGTGACTGTGTGATATCGGTTTTatcc                                                                                                                                                                                                                                       <  2:405701/251‑4 (MQ=255)
                              attattTGATTCAGCGGCGTGAAAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCCATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTC‑TGTCTACATCCGCCCGACTCTACAGTACACACAGCAGTGCTTCCGCGTCTTAAATCCGGCATCGTCTCCTCTGCGCCGCCGACTGTATTATCACAGTTTTCACCTTTAGTTGCCAATTTTCTTCAGTGAc                                                                                                                                                                                                             >  1:400526/1‑247 (MQ=255)
                                 attggagtCAGCGGCGTGATTGTCGCCAGACACCTCTTACCGCGTCATTAATCACTCAATTCCGGCGATAATGCGGCTATCTTCAAAGTTAAATCTGTAGAAAAAGATATAGTC‑GGTCGACATCCGCCAGACTCGACATTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTTTCTCCTCTGCGCCGGGGACTGTATTATAGCGGTTTTAACCCTTAGTTTCCAATTTGCTTCAGTTACAGAttt                                                                                                                                                                                                       <  2:677928/243‑1 (MQ=255)
                                             ggcgTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTC‑TGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAAtata                                                                                                                                                                                           >  1:557956/1‑250 (MQ=255)
                                                gTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTC‑TGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGtt                                                                                                                                                                                       <  1:554030/251‑1 (MQ=255)
                                                  gATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTC‑TGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTAc                                                                                                                                                                                     >  1:66188/1‑251 (MQ=255)
                                                     aGTCGCCAGACCCCACTTACCCCGTACTTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTC‑TGGCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACatt                                                                                                                                                                                  <  2:610065/251‑1 (MQ=255)
                                                       cctccAGACACCACTGACCGCGTCATTAATCACGCAATACCGGCGAGAATGCGGCTATCTTCAAAGGTAAAACTGCAGAAAAAAATAGAGTC‑TGTCGAAATCCGCCAGACTCTACAGTACACACAGCAGGTCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTAGAACGGTTTTAACCTGTAGTTGCCAATTTTCTTCATTGACAGATTTCACGAATATATAAGTTACattat                                                                                                                                                                                <  2:669811/248‑1 (MQ=255)
                                                                                  aaTCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTC‑TGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACgg                                                                                                                                                                                                                                               >  1:382587/1‑161 (MQ=255)
                                                                                                      agtgccgtgaTCTGGAAAGTTAAAACTGCTTAAAAAAAT‑GGGTGGTGTCCACAGCCGCCAGACTCTACAGGGCTCACAGCAGTGCAACCGCGTCGTAAATCTGGCAGTGTCTCCGCTGCGCCGGGGACTGTAGTATAACGGTTTTAACCTTTAGTTGTCAAATTGCTTCAGTGACAGATTTCACGAAAATATAAGTAACATTATTAATATCGTGAAGGGATAATCATGCTTAAGTaagttgtataaaatat                                                                                                                                 <  2:300288/242‑16 (MQ=255)
                                                                                                      aaTGCGTCTATCGTCAAAGTCAAAACTGCATAAAAAAAGAGTGTC‑TGTCGATCTCCGCCAGACTCTACAGTTAACACAGCAGTGCATCCGCGTCATATATCCGGCATTGTCTCCTCTGCTCTGGTGAATGTAATATTACGGTCTTAACCTTTTGTTGCCAATTTTGTTCAGTGATATATTTCACGAAAATATAAGTTACATTATTAATTTCGTGCATGAATAATCATGCATAAGTATTTTGCTTAAAatat                                                                                                                                 <  2:23600/251‑1 (MQ=255)
                                                                                                                   gCAAAGATATAACTGTAAAAAAAAATAGAGTC‑TGTCGCCATTCGCCATGCACGTCAGTACACACCGCAGTCCAGCCGCGTCTTAAATCCGGTATTGTCTCCTCGGCGCCGGTGACTGTATTATTACTGATTTAACTTTTAGTTGCCAATTTTCTGCAGGGACATATTTCACGAAAATATAAGTTACATTATTAATAGCGTGAAGTAATAATCATGCTTAAGTATTTTGCGTAAAATATCGGCAatatgt                                                                                                                      <  2:400526/249‑3 (MQ=255)
                                                                                                                                   aaaaaaaatTAGAGTC‑TGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGACTTAAATCCTGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATCTCACGAAAATAGAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTGGCTTAAAATATCGGCAATATTTGGAACTTATGACTGGaaa                                                                                                    <  2:2501/249‑1 (MQ=255)
                                                                                                                                       aaaaaTAGAGTC‑TGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTg                                                                                                <  1:475701/251‑1 (MQ=255)
                                                                                                                                             agagTC‑TGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACa                                                                                                                                                                                                            <  1:113136/137‑1 (MQ=255)
                                                                                                                                             agagTC‑TGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACa                                                                                                                                                                                                            >  2:113136/1‑137 (MQ=255)
                                                                                                                                              gagTC‑TGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGAGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGAATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAAt                                                                                          >  2:581202/1‑250 (MQ=255)
                                                                                                                                               agTC‑TGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAGTCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTCTTAATCTCGTGAATGAATAATAATGCATAAGACTTTTGCTTAAAATATCGGCATTCTTTGGAACTTCTTCCTGAAACTTTGGGTAATAc                                                                                        >  2:305130/1‑251 (MQ=255)
                                                                                                                                                       cGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGttgttg                                                                                 >  1:131881/1‑251 (MQ=255)
                                                                                                                                                       cGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTGTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGttgttg                                                                                 <  1:586550/251‑1 (MQ=255)
                                                                                                                                                        gACATCCGCCAGAATCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTgg                                                                                >  1:529110/1‑251 (MQ=255)
                                                                                                                                                                                 cacaGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATc                                                       <  1:508353/251‑1 (MQ=255)
                                                                                                                                                                                 cacaGCAGTGCATCCGCGTCTTAAAACCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGAGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGACTGAATAATCATGAATAAGTATTTTGCTTAAACTATCGACAATATTTGGAACTTATTACTGCAAATTTGGTCAATACGTTGTTGGACCTAACCGGACTGGTTATCatat                                                        >  2:524797/1‑250 (MQ=255)
                                                                                                                                                                                    agcagTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCACTTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGa                                                                                                                   >  2:213714/1‑188 (MQ=255)
                                                                                                                                                                                    agcagTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGa                                                                                                                   <  1:213714/188‑1 (MQ=255)
                                                                                                                                                                                                   tCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGAATGTATTATAACGGTTTTACCCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCggg                                      >  2:391929/1‑250 (MQ=255)
                                                                                                                                                                                                                   gTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAAcc                     >  1:56353/1‑251 (MQ=255)
                                                                                                                                                                                                                               ccGGTGCCTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATAATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCAGGTCTGGTTATCATATCGCGCTTTTAATTGCGGGAGGATGTAACATGAACCTTgacaccaaat         >  2:198498/1‑244 (MQ=255)
                                                                                                                                                                                                                                cGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATc        >  1:69488/1‑251 (MQ=255)
                                                                                                                                                                                                                                     aCTGTATTATAACGAATTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTACAATGAACCTTGACGACAAATCGATGt   >  1:526786/1‑251 (MQ=255)
                                                                                                                                                                                                                                      cTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGtt  >  1:117592/1‑251 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      
CGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTC‑TGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTT  >  NC_000913/1405525‑1406004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑