Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,323,798	G→A	K193K (AAG→AAA)	rnm →	RNase AM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,323,798	0	G	A	100.0%	192.9 / NA	61	K193K (AAG→AAA)	rnm	RNase AM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/32);  total (29/32)

GCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAGGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTT  >  NC_000913/1323555‑1324044                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        gCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGc                                                                                                                                                                                                                                                  <  1:62768/251‑1 (MQ=255)    gATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACACCGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCt                                                                                                                                                                                                                                                 >  1:409665/1‑249 (MQ=255)     aTTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGc                                                                                                                                                                                                                                              >  1:197808/1‑251 (MQ=255)          cGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATc                                                                                                                                                                                                                                         <  1:528752/251‑1 (MQ=255)             aaGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTg                                                                                                                                                                                                                                      <  1:135479/251‑1 (MQ=255)                        aaGCGCAGATACGTGGGGCGCCGGTATGCGTGCATCGACTGGCGCAGGGCTGCTCTGTGACGCGCGGTCATGTTTCGGTTTTCCTTGTTGATTGAGGCAAAGCTCGTCCATTTTCTGATGTTTTTAAAAAGGATTTTGCGCGTGGGAAAATTGGATACTTTTCGCCACAGTGGTGTACAATAGAACAAGCTATTGATTTCATTCATCATTCGGGCGGTAAATCGGTGCTAGCCCATCCTGGGCGGTACAAt                                                                                                                                                                                                                           <  2:679830/251‑1 (MQ=255)                           cgcAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGGGCTAGCTCATCCTGGGCGGTACAATCtt                                                                                                                                                                                                                        >  1:172562/1‑251 (MQ=255)                                   ccTGGGGCGCTGGAAGGCGCACATCGATTGGCGCAGGGCGGCGCAGGGACGCGCTGTCATTTTGCGCGTTTCCTTGTTGAGTGCGTCAAAGCCAGTTCCATGGCGGATGGCTTTAAAAAGTATCTGGCGCGCGGTAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTATTGCTaa                                                                                                                                                                                                                <  2:563313/251‑1 (MQ=255)                                      ggggCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTTCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTaaa                                                                                                                                                                                                               <  1:563363/249‑1 (MQ=255)                                      ggggCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATg                                                                                                                                                                                                             >  1:90187/1‑251 (MQ=255)                                            cTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGAGGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGAGGTGTACAATAGAACAAGCTATTGATGGCATTCATCATTCTGTCGGTAAAACGGTGGTAGCTCATCCTGGGCGGGACAATCTTAGTGCTAAATGGGTGa                                                                                                                                                                                                        >  2:200688/1‑250 (MQ=255)                                                            gACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCAt                                                                                                                                                                                       >  1:155611/1‑251 (MQ=255)                                                                     aGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGGGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTTGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTACCGa                                                                                                                                                                               >  2:625646/1‑250 (MQ=255)                                                                              cAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACggg                                                                                                                                                                    >  1:12106/1‑250 (MQ=255)                                                                                gTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGGGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCCTCGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTg                                                                                                                                                                   >  2:177728/1‑250 (MQ=255)                                                                                 ggACGCGCGTTCATTTTGCGCGTTTCCTTGGTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGAc                                                                                                                                                                 <  2:172562/250‑1 (MQ=255)                                                                                  gACGCGCGGTCTTTTTGTGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGAcg                                                                                                                                                                <  2:90187/251‑1 (MQ=255)                                                                                            cATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGt                                                                                                                                                      >  1:295046/1‑251 (MQ=255)                                                                                            cATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGAGCTAGCTCAACCTGGGCGGTACAATCTTAGTGCTAAATGGCTGGAAAGATTGGGTGCGCATTTTGCCGACCAC‑CACGGGGACGCGCTgg                                                                                                                                                          >  2:163537/1‑247 (MQ=255)                                                                                            cATTTTGCGCGTTTCCTTGGTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCAGCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGt                                                                                                                                                      <  2:80077/251‑1 (MQ=255)                                                                                                 tGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCCCAGTGGTGTACAATAGACCAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAACGGGGCCAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGGTGGAAAGATTGGTTGGGCATTTTGCCGAACAC‑CACGGTGACGCGATGGagaacacgc                                                                                                                                                 >  2:560975/1‑243 (MQ=255)                                                                                                                tgAGTGCGGCAAATCCAGTTCAATGGCGGATGTTTTTAAAAAGTATCTGGCTCGCGGGAAAACCGGATACGGTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGGGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCg                                                                                                                                   <  2:618267/250‑1 (MQ=255)                                                                                                                              ccAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTAcc                                                                                                                    <  1:242923/251‑1 (MQ=255)                                                                                                                                        ggCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTggcg                                                                                                           <  1:299721/250‑1 (MQ=255)                                                                                                                                          cGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAAGCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGcc                                                                                                        >  2:523591/1‑251 (MQ=255)                                                                                                                                           ggATGTCTTTAAAACGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCCTTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGccc                                                                                                       >  1:457798/1‑251 (MQ=255)                                                                                                                                             aTGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCtt                                                                                                     <  1:625646/251‑1 (MQ=255)                                                                                                                                                           tATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGcatc                                                                                        >  1:335744/1‑250 (MQ=255)                                                                                                                                                             tCTGGCGCGCGGGAAAACCGGTTCCGGTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCTCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGcatcat                                                                                      <  2:197808/250‑1 (MQ=255)                                                                                                                                                              ccgccgacatggAAAACCGGTTACGTCCCGCCCCTCGGGTGTACCATAGTACAAGCTACTGCAGTCATTCATCATCCTGGCGGTAAAGCGTTGCTAGCACATCCTTGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGAGTGGTTGCCCACTTTGCCGAACAC‑CACGGTGCAGCGATGGAAGTCGCGCAGTGCCAGCAATCTCCCAATTAACGTACCCAGCTGGCTGTCCTTTCCCGTCAGcatcttt                                                                                     <  2:497103/240‑4 (MQ=255)                                                                                                                                                                ggCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTa                                                                                   <  1:144580/250‑1 (MQ=255)                                                                                                                                                                    cgcgGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGAGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCCTTTATGGGc                                                                              >  2:98496/1‑251 (MQ=255)                                                                                                                                                                      ggggAAAACCGGATACGGTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCAt                                                                            <  2:41581/250‑1 (MQ=255)                                                                                                                                                                       ggtaaaaCCGGATACGTTCCGCCACAGTGGTGTACAATATAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGTTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCATTGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCAGCATTTATGGGCATc                                                                           <  2:447420/248‑1 (MQ=255)                                                                                                                                                                        ggAAAACCGGATCCGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGAGTGGTTGCGCATTTCGCCGAACAC‑CTCGTTGAc                                                                                                                                                                 <  2:60014/164‑1 (MQ=255)                                                                                                                                                                        ggAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACCATCTTAGTGCTAACTGGCTGAAAAGATTGGTTGCGCATTTTGCCGCACAC‑CACGGTGAc                                                                                                                                                                 >  1:60014/1‑164 (MQ=255)                                                                                                                                                                          aaaaCCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGCCGCGATGGAAATCGCGCAGTGCCAGCAATCGCCAAATGAACGAACCCAGCTGGGAGCCCTTGCGCGTCAGCATCATTTATGGGCaaccc                                                                         >  2:103271/1‑246 (MQ=255)                                                                                                                                                                          aaaaCCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATcac                                                                         >  1:490378/1‑250 (MQ=255)                                                                                                                                                                             acctgATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCTCAAgg                                                                     <  2:120815/247‑1 (MQ=255)                                                                                                                                                                             aCCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAgg                                                                     <  1:275927/251‑1 (MQ=255)                                                                                                                                                                               cGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGAt                                                                   >  1:341138/1‑251 (MQ=255)                                                                                                                                                                                ggATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGATGCGCTGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATc                                                                  >  1:355626/1‑251 (MQ=255)                                                                                                                                                                                      gTTCCGCCCCAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCATCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGAttt                                                            <  2:433855/251‑1 (MQ=255)                                                                                                                                                                                        tCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGAtttt                                                           <  1:98496/250‑1 (MQ=255)                                                                                                                                                                                          cGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGTGCATCACAAGGATCTGATTTtcat                                                        <  1:68921/251‑1 (MQ=255)                                                                                                                                                                                            ccACAGTGGTTTACAATAGAACAAGCTATTGATGTCATTCATCATGCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCAGCATTTATGGGCATCACAAGGATCTGACTTtcatca                                                      <  2:341138/251‑1 (MQ=255)                                                                                                                                                                                              acaGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGc                                                    <  1:202283/251‑1 (MQ=255)                                                                                                                                                                                                 gtggtgTACAATAGTACAAGCTATTTAATTCATTCATCATTCTGGCGGTAAAGCGGTGATAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCTCACAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCAt                                                 <  2:464868/251‑1 (MQ=255)                                                                                                                                                                                                  tggtgTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATg                                                >  1:306150/1‑251 (MQ=255)                                                                                                                                                                                                    gtgtACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCCTCATTTATGGGCATCCCAAGGATCTGATTTTCATCAGCCAtacc                                              >  2:264976/1‑248 (MQ=255)                                                                                                                                                                                                     tgtACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGccc                                             >  1:624691/1‑251 (MQ=255)                                                                                                                                                                                                        aCAATAGAACAAGCTATTGATGTGATTCATCATTGATGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGTAAAGATTGGTTGCGCAGTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCTCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCAACAGCCATGCCCGTg                                          <  2:233178/251‑1 (MQ=255)                                                                                                                                                                                                        aCAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTg                                          <  1:163537/251‑1 (MQ=255)                                                                                                                                                                                                          actAGAACAAGCTATTGATGTAATTCATCATTCTGGCGGTAAAACGGAGCTAGCTCATCCTGGGCTGGACAATCTTAGTGCTAAATGGCTGAAAAGAGTGGTTGCGCATTTTGCAGAA‑ACACACGGTTACGCGCTGGAAGTCGCGCATGGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGTCCCTGCGCGTCAGCATAATTTATGGGCATCAAGAGGATCTGCTTTtcatacgtcactcccatgg                                         >  2:199366/3‑235 (MQ=255)                                                                                                                                                                                                             aGAACAAGCTATTGATTTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATc                                      <  2:306150/250‑1 (MQ=255)                                                                                                                                                                                                                 cAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACt                                 >  1:386306/1‑251 (MQ=255)                                                                                                                                                                                                                      tATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTACGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTc                            >  1:315186/1‑251 (MQ=255)                                                                                                                                                                                                                        tttaTGTCATTCATCATGCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCGTAAAAGATTGGTTGCGCATTTTGCCGAGCAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCTCCCAATGAACGTACCCAGCTGGCGGCCCTGGCGCGTCAGCATCATTTATGGGCGTCACAAGGATCGGATTTGCATCAGCTATTCCCTTGGATCGAACTTGGTCg                           <  2:637150/247‑1 (MQ=255)                                                                                                                                                                                                                         tGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGt                          <  1:177728/250‑1 (MQ=255)                                                                                                                                                                                                                              tcattcatCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAActc                    >  1:595432/1‑251 (MQ=255)                                                                                                                                                                                                                                               ggTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCT‑GCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGtt  >  1:165076/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        GCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAGGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACAC‑CACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTT  >  NC_000913/1323555‑1324044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑